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Shimadzu Corporation liquid chromatography system
Liquid Chromatography System, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid chromatography system/product/Shimadzu Corporation
Average 92 stars, based on 109 article reviews
Price from $9.99 to $1999.99
liquid chromatography system - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Anthocyanin Accumulation in Black Kernel Mutant Rice and its Contribution to ROS Detoxification in Response to High Temperature at the Filling Stage
Article Snippet: .. HPLC analysis was performed using a liquid chromatography system (SCL-10A, Shimadzu, Kyoto, Japan) equipped with two pumps (LC-10A), a control system (SCL-10A), a diode array detector (SPD-20A), a CTA-20A column oven and a column COSMOSIL 5C18-AR-II 4.66250 mm i.e., 5 mm (Nacalaitesque, Kyoto, Japan). ..

Article Title: Genetic mapping of ovary colour and quantitative trait loci for carotenoid content in the fruit of Cucurbita maxima Duchesne
Article Snippet: .. The samples were homogenised in liquid nitrogen and 100 mg of the homogenate was used for carotenoid extraction and HPLC analysis with the use of the Shimadzu Liquid Chromatography System (Shimadzu, Japan). .. The pigments were separated on a Synergi 4u MAX-RP 80A 250 × 4.6 column (Phenomenex, USA) at 30 °C.

Article Title: Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs
Article Snippet: .. HPLC purification of exosomes The instrument setup consisted of a liquid chromatography system with a binary pump, an auto injector, a thermostated column oven and a UV-visible detector operated by the Class VP software from Shimadzu Corporation (Kyoto, Japan). .. The Chromatography columns used were TSK Guard column SWXL, 6 × 40 mm and TSK gel G4000 SWXL, 7.8 × 300 mm from Tosoh Corporation (Tokyo, Japan).

Flow Cytometry:

Article Title: Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity
Article Snippet: .. Chromatography was performed on a Shimadzu liquid chromatography system using a Thermo Aquasil C-18 column (150 × 4.6 mm, 5 μ m particle size) at a flow rate of 0.25 mL minute−1 . .. The system was coupled to the Shimadzu ion trap-time of flight (IT-TOF) mass spectrometer with an electrospray (ESI) source and consisted of two LC-20AD pumps with UFLC-XR upgrade, SIL-20ACHT autosampler, CTO-20A column oven, DGU-20A3 degasser, and CBM-20A communications module.

Filtration:

Article Title: Atomic view of the energy landscape in the allosteric regulation of Abl kinase
Article Snippet: .. MALS Multiangle light scattering was measured using a DAWN HELEOS-II (Wyatt Technology Corporation) downstream of a Shimadzu liquid chromatography system connected to a Superdex 200 10/300 GL (GE Healthcare) gel filtration column. ..

Chromatography:

Article Title: Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity
Article Snippet: .. Chromatography was performed on a Shimadzu liquid chromatography system using a Thermo Aquasil C-18 column (150 × 4.6 mm, 5 μ m particle size) at a flow rate of 0.25 mL minute−1 . .. The system was coupled to the Shimadzu ion trap-time of flight (IT-TOF) mass spectrometer with an electrospray (ESI) source and consisted of two LC-20AD pumps with UFLC-XR upgrade, SIL-20ACHT autosampler, CTO-20A column oven, DGU-20A3 degasser, and CBM-20A communications module.

Purification:

Article Title: Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs
Article Snippet: .. HPLC purification of exosomes The instrument setup consisted of a liquid chromatography system with a binary pump, an auto injector, a thermostated column oven and a UV-visible detector operated by the Class VP software from Shimadzu Corporation (Kyoto, Japan). .. The Chromatography columns used were TSK Guard column SWXL, 6 × 40 mm and TSK gel G4000 SWXL, 7.8 × 300 mm from Tosoh Corporation (Tokyo, Japan).

Concentration Assay:

Article Title: Vitamin D Status at Birth and Future Risk of Attention Deficit/Hyperactivity Disorder (ADHD)
Article Snippet: .. Analysis of vitamin D concentration Quantitative analyses of cord sera were conducted using a triple quadrupole linear ion trap mass spectrometer (QTRAP 5500; AB Sciex, Foster City, CA, USA) coupled to a liquid chromatography system (UFLCXR, Shimadzu Corporation, Kyoto, Japan; LC/MS/MS). .. Vitamin D concentrations were determined by a modified method for analysis of perfluorinated compounds [ ].

Mass Spectrometry:

Article Title: Vitamin D Status at Birth and Future Risk of Attention Deficit/Hyperactivity Disorder (ADHD)
Article Snippet: .. Analysis of vitamin D concentration Quantitative analyses of cord sera were conducted using a triple quadrupole linear ion trap mass spectrometer (QTRAP 5500; AB Sciex, Foster City, CA, USA) coupled to a liquid chromatography system (UFLCXR, Shimadzu Corporation, Kyoto, Japan; LC/MS/MS). .. Vitamin D concentrations were determined by a modified method for analysis of perfluorinated compounds [ ].

Article Title: The Effect of CM082, an Oral Tyrosine Kinase Inhibitor, on Experimental Choroidal Neovascularization in Rats
Article Snippet: .. An API-4000 triple-quadrupole mass spectrometer (AB Sciex, Framingham, MA, USA) coupled with a Shimadzu liquid chromatography system (Shimadzu Cooperation, Kyoto, Japan) was used for the analysis. ..

Article Title: Topical, Aqueous, Clear Cyclosporine Formulation Design for Anterior and Posterior Ocular Delivery
Article Snippet: .. The LC-MS/MS comprised a triple-quadrupole mass spectrometer with SCIEX API 4000 (API 4000; Applied Biosystems/MDI SCIEX, Foster City, CA) coupled to a liquid chromatography system (LC-10 AD; Shimadzu Scientific Instruments) and reversed-phase ACE 5 phenyl column, 50 × 2.1 mm × 5 μm (Advanced Chromatography Technologies, Ltd., Aberdeen Scotland, UK), and a guard column (ACE 5 phenyl, 10 × 2.1 mm, 5 μm; Thermo-Hypersil Keystone, City, ST). .. Column temperature was maintained at 70°C (Eppendorf CH-30 column heater; Sigma Aldrich, St. Louis, MO).

Liquid Chromatography:

Article Title: Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity
Article Snippet: .. Chromatography was performed on a Shimadzu liquid chromatography system using a Thermo Aquasil C-18 column (150 × 4.6 mm, 5 μ m particle size) at a flow rate of 0.25 mL minute−1 . .. The system was coupled to the Shimadzu ion trap-time of flight (IT-TOF) mass spectrometer with an electrospray (ESI) source and consisted of two LC-20AD pumps with UFLC-XR upgrade, SIL-20ACHT autosampler, CTO-20A column oven, DGU-20A3 degasser, and CBM-20A communications module.

Article Title: Vitamin D Status at Birth and Future Risk of Attention Deficit/Hyperactivity Disorder (ADHD)
Article Snippet: .. Analysis of vitamin D concentration Quantitative analyses of cord sera were conducted using a triple quadrupole linear ion trap mass spectrometer (QTRAP 5500; AB Sciex, Foster City, CA, USA) coupled to a liquid chromatography system (UFLCXR, Shimadzu Corporation, Kyoto, Japan; LC/MS/MS). .. Vitamin D concentrations were determined by a modified method for analysis of perfluorinated compounds [ ].

Article Title: Anthocyanin Accumulation in Black Kernel Mutant Rice and its Contribution to ROS Detoxification in Response to High Temperature at the Filling Stage
Article Snippet: .. HPLC analysis was performed using a liquid chromatography system (SCL-10A, Shimadzu, Kyoto, Japan) equipped with two pumps (LC-10A), a control system (SCL-10A), a diode array detector (SPD-20A), a CTA-20A column oven and a column COSMOSIL 5C18-AR-II 4.66250 mm i.e., 5 mm (Nacalaitesque, Kyoto, Japan). ..

Article Title: The Effect of CM082, an Oral Tyrosine Kinase Inhibitor, on Experimental Choroidal Neovascularization in Rats
Article Snippet: .. An API-4000 triple-quadrupole mass spectrometer (AB Sciex, Framingham, MA, USA) coupled with a Shimadzu liquid chromatography system (Shimadzu Cooperation, Kyoto, Japan) was used for the analysis. ..

Article Title: Genetic mapping of ovary colour and quantitative trait loci for carotenoid content in the fruit of Cucurbita maxima Duchesne
Article Snippet: .. The samples were homogenised in liquid nitrogen and 100 mg of the homogenate was used for carotenoid extraction and HPLC analysis with the use of the Shimadzu Liquid Chromatography System (Shimadzu, Japan). .. The pigments were separated on a Synergi 4u MAX-RP 80A 250 × 4.6 column (Phenomenex, USA) at 30 °C.

Article Title: Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs
Article Snippet: .. HPLC purification of exosomes The instrument setup consisted of a liquid chromatography system with a binary pump, an auto injector, a thermostated column oven and a UV-visible detector operated by the Class VP software from Shimadzu Corporation (Kyoto, Japan). .. The Chromatography columns used were TSK Guard column SWXL, 6 × 40 mm and TSK gel G4000 SWXL, 7.8 × 300 mm from Tosoh Corporation (Tokyo, Japan).

Article Title: Atomic view of the energy landscape in the allosteric regulation of Abl kinase
Article Snippet: .. MALS Multiangle light scattering was measured using a DAWN HELEOS-II (Wyatt Technology Corporation) downstream of a Shimadzu liquid chromatography system connected to a Superdex 200 10/300 GL (GE Healthcare) gel filtration column. ..

Article Title: Topical, Aqueous, Clear Cyclosporine Formulation Design for Anterior and Posterior Ocular Delivery
Article Snippet: .. The LC-MS/MS comprised a triple-quadrupole mass spectrometer with SCIEX API 4000 (API 4000; Applied Biosystems/MDI SCIEX, Foster City, CA) coupled to a liquid chromatography system (LC-10 AD; Shimadzu Scientific Instruments) and reversed-phase ACE 5 phenyl column, 50 × 2.1 mm × 5 μm (Advanced Chromatography Technologies, Ltd., Aberdeen Scotland, UK), and a guard column (ACE 5 phenyl, 10 × 2.1 mm, 5 μm; Thermo-Hypersil Keystone, City, ST). .. Column temperature was maintained at 70°C (Eppendorf CH-30 column heater; Sigma Aldrich, St. Louis, MO).

Software:

Article Title: Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs
Article Snippet: .. HPLC purification of exosomes The instrument setup consisted of a liquid chromatography system with a binary pump, an auto injector, a thermostated column oven and a UV-visible detector operated by the Class VP software from Shimadzu Corporation (Kyoto, Japan). .. The Chromatography columns used were TSK Guard column SWXL, 6 × 40 mm and TSK gel G4000 SWXL, 7.8 × 300 mm from Tosoh Corporation (Tokyo, Japan).

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  • 88
    Shimadzu Corporation hplc elsd analysis hplc analysis
    <t>HPLC–ELSD</t> chromatograms of ( a ) 0 h control incubation with ADS-I; ( b ) 72 h control incubation without ADS-I; ( c ) 24 h incubation with ADS-I; ( d ) 72 h incubation with ADS-I.
    Hplc Elsd Analysis Hplc Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc elsd analysis hplc analysis/product/Shimadzu Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc elsd analysis hplc analysis - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    93
    Shimadzu Corporation sy0916
    Content of <t>SY0916,</t> D1, and D2 in buffer solutions (pH 3.0∼5.0) and water over 24 h at room temperature.
    Sy0916, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sy0916/product/Shimadzu Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sy0916 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    85
    Shimadzu Corporation mito chmac
    Intracellular accumulation of <t>Mito-ChM</t> in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM <t>Mito-ChMAc.</t> ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.
    Mito Chmac, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mito chmac/product/Shimadzu Corporation
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mito chmac - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Shimadzu Corporation protein sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page
    <t>SDS-PAGE</t> results of rhGH encapsulated in various microspheres. Lane 1: Standard protein markers, Lane 2: Standard rhGH, Lane 3: Standard rhGH complexed with zinc (1:100 molar ratio), Lanes 4-7: F, G, H and I microspheres, respectively, fabricated by method II, Lane 8: E microspheres fabricated by method I
    Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page/product/Shimadzu Corporation
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    protein sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page - by Bioz Stars, 2020-09
    85/100 stars
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    HPLC–ELSD chromatograms of ( a ) 0 h control incubation with ADS-I; ( b ) 72 h control incubation without ADS-I; ( c ) 24 h incubation with ADS-I; ( d ) 72 h incubation with ADS-I.

    Journal: Molecules

    Article Title: Ardipusilloside-I Metabolites from Human Intestinal Bacteria and Their Antitumor Activity

    doi: 10.3390/molecules201119719

    Figure Lengend Snippet: HPLC–ELSD chromatograms of ( a ) 0 h control incubation with ADS-I; ( b ) 72 h control incubation without ADS-I; ( c ) 24 h incubation with ADS-I; ( d ) 72 h incubation with ADS-I.

    Article Snippet: HPLC–ELSD Analysis HPLC analysis of ADS-I metabolites were carried out using an LC-20A high performance liquid chromatograph (Shimadzu Corporation, Kyoto, Japan) equipped with a Alltech type 3300 evaporative light-scattering detector (Alltech Associates, Deerfield, MA, USA).

    Techniques: High Performance Liquid Chromatography, Incubation

    Content of SY0916, D1, and D2 in buffer solutions (pH 3.0∼5.0) and water over 24 h at room temperature.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Degradation Characteristics of a Novel PAF Receptor Antagonist, SY0916, in Aqueous Solution

    doi: 10.1155/2019/8789470

    Figure Lengend Snippet: Content of SY0916, D1, and D2 in buffer solutions (pH 3.0∼5.0) and water over 24 h at room temperature.

    Article Snippet: HPLC Analysis The separation of SY0916 and its DPs was achieved using a Shimadzu LC-20AD liquid chromatography system with an autoinjector, binary solvent manager, and photodiode array (PDA) detector (Shimadzu, Tokyo, Japan).

    Techniques:

    Structures of (a) SY0916, (b) D1, and (c) D2.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Degradation Characteristics of a Novel PAF Receptor Antagonist, SY0916, in Aqueous Solution

    doi: 10.1155/2019/8789470

    Figure Lengend Snippet: Structures of (a) SY0916, (b) D1, and (c) D2.

    Article Snippet: HPLC Analysis The separation of SY0916 and its DPs was achieved using a Shimadzu LC-20AD liquid chromatography system with an autoinjector, binary solvent manager, and photodiode array (PDA) detector (Shimadzu, Tokyo, Japan).

    Techniques:

    Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Multiple Displacement Amplification, High Performance Liquid Chromatography, Concentration Assay, Incubation, Mass Spectrometry

    Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Staining

    SDS-PAGE results of rhGH encapsulated in various microspheres. Lane 1: Standard protein markers, Lane 2: Standard rhGH, Lane 3: Standard rhGH complexed with zinc (1:100 molar ratio), Lanes 4-7: F, G, H and I microspheres, respectively, fabricated by method II, Lane 8: E microspheres fabricated by method I

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: An Approach to the Design of a Particulate System for Oral Protein Delivery .II. Preparation and Stability Study of rhGH-Loaded Microspheres in Simulated Gastrointestinal Fluids

    doi:

    Figure Lengend Snippet: SDS-PAGE results of rhGH encapsulated in various microspheres. Lane 1: Standard protein markers, Lane 2: Standard rhGH, Lane 3: Standard rhGH complexed with zinc (1:100 molar ratio), Lanes 4-7: F, G, H and I microspheres, respectively, fabricated by method II, Lane 8: E microspheres fabricated by method I

    Article Snippet: Determination of chemical stability and aggregation of protein Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using a VEU-7709 vertical tank and EPS-7602 power supply (Payapajoohesh Co., Iran) and reversed-phase high performance liquid chromatography (RP-HPLC) was carried out using a computer-controlled Shimadzu HPLC system (Japan) equipped with two pumps (LC-10 ADvp), a system controller (SCL-10Avp), a degasser ((DGU-14A) and a diode array detector (SPD-M10vp).

    Techniques: SDS Page