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Shimadzu Corporation liquid chromatography mass spectrometry
Liquid Chromatography Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid chromatography mass spectrometry/product/Shimadzu Corporation
Average 93 stars, based on 44 article reviews
Price from $9.99 to $1999.99
liquid chromatography mass spectrometry - by Bioz Stars, 2020-09
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High Performance Liquid Chromatography:

Article Title: Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Article Snippet: .. The purity of the peptides was determined using HPLC (Shimadzu, Kyoto, Japan) and liquid chromatography-mass spectrometry (LC-MS, Shimadzu, Kyoto, Japan). ..

Mass Spectrometry:

Article Title: Development and Phytochemical Characterization of High Polyphenol Red Lettuce with Anti-Diabetic Properties
Article Snippet: .. Liquid Chromatography Mass Spectrometry (LCMS) Methanolic extract samples were separated and analyzed with a Shimadzu LCMS-2010A high performance liquid chromatography/electrospray ionization/single quadrupole mass spectrometer. .. The LCMS is equipped with two pumps (LC-10ADvp), controller (SCL-10Avp), auto-injector (SIL-10ADvp), column oven (CTO-10ACvp), photodiode array detector (SPD-M10Avp), and a single quadrupole analyzer.

Article Title: Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea
Article Snippet: .. Liquid Chromatography Mass Spectrometry The analyzes were conducted using a Shimadzu Prominence UFLC system (Shimadzu, Kyoto, Japan). ..

Article Title: New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
Article Snippet: .. Chemical Characterization of TPC and the Novel TPC–Peptide Conjugates Verification of the relative atomic mass of the conjugates was carried out using Liquid Chromatography Mass Spectrometry (LCMS) on a LCMS-2010 EV (Shimadzu Corporation, Marne-la-Vallée, Paris, France) equipped with a diode array detector SPD-M20A, a column oven CT0-20AC and a DGU-20A3degasser.1 H NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer (Bruker, Wissenburg, Germany). .. All samples were prepared in deuterated dimethyl sulfoxide (DMSO-d 6 ) with a concentration of about 10 mM.

Chromatography:

Article Title: Development and Phytochemical Characterization of High Polyphenol Red Lettuce with Anti-Diabetic Properties
Article Snippet: .. Liquid Chromatography Mass Spectrometry (LCMS) Methanolic extract samples were separated and analyzed with a Shimadzu LCMS-2010A high performance liquid chromatography/electrospray ionization/single quadrupole mass spectrometer. .. The LCMS is equipped with two pumps (LC-10ADvp), controller (SCL-10Avp), auto-injector (SIL-10ADvp), column oven (CTO-10ACvp), photodiode array detector (SPD-M10Avp), and a single quadrupole analyzer.

Article Title: Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Article Snippet: .. The purity of the peptides was determined using HPLC (Shimadzu, Kyoto, Japan) and liquid chromatography-mass spectrometry (LC-MS, Shimadzu, Kyoto, Japan). ..

Article Title: Dynamic Phosphorylation of the C Terminus of Hsp70 Regulates the Mitochondrial Import of SOD2 and Redox Balance
Article Snippet: .. Mitochondrial O2 and H2 O2 were measured using liquid chromatography-mass spectrometry (LC-MS/MS, Shimadzu Nexera UHPLC system). .. For mitochondrial O2 measurement, samples from lungs, distal PAs and cells were incubated with mitoSOX (5 μM in fresh culture medium at 37°C for 30 min).

Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
Article Snippet: .. Reactions were monitored by thin-layer chromatography (TLC) using Merck 60F254 silica gel plates or by Liquid Chromatography-Mass Spectrometry on Shimadzu LCMS-2020 with either Waters, XSelect HSS CYANO 3.6 µm column or Agilent XBD-C18 3.5 μm column; dimensions 2.1 × 20 mm and UV detector at 254 nM. .. Chromatography purification was performed on Biotage Isolera Four instrument using pre-filled KP-Sil (normal phase) and KP-C18-HS (reverse phase) SNAP cartridges with UV detection at 254 and 280 nm.

Article Title: Non‐nucleoside reverse transcriptase inhibitor levels among HIV‐exposed uninfected infants at the time of HIV PCR testing – findings from a tertiary healthcare facility in Pretoria, South AfricaNon‐nucleoside reverse transcriptase inhibitor levels among HIV‐exposed uninfected infants at the time of HIV PCR testing – findings from a tertiary healthcare facility in Pretoria, South Africa
Article Snippet: .. 2.3 Laboratory methods to assess ARV levels Quantitative plasma EFV and NVP drug level testing was performed on all samples, irrespective of maternal cART regimen, using liquid chromatography‐mass spectrometry (Shimadzu 8060). .. Matrix matched standards and controls (Chromsystems, Munich, DE) were used to create a 7‐point standard curve.

Concentration Assay:

Article Title: Robustness of anaerobes exposed to cyanuric acid contaminated wastewater and achieving efficient removal via optimized co-digestion scheme
Article Snippet: .. CA concentration was determined using liquid chromatography-mass spectrometry (LCMS-2020, Shimadzu) with an APCI interface on negative polarity. .. The measurement conditions are as follows; i.e., shim-pack XR-ODS II column with a size of 3 × 100 mm was used; the mobile phase was 90% acetonitrile and 10% of 0.1 formic acid solution at a flow rate of 0.4 mL/min; the oven temperature was 30 °C, and the retention time was 5 min. Scanning electron microscopy (SEM) analysis for sludge samples was performed to examine the surface morphology change after long-term exposure to CA (Hitachi S-4500, Japan).

Nuclear Magnetic Resonance:

Article Title: New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
Article Snippet: .. Chemical Characterization of TPC and the Novel TPC–Peptide Conjugates Verification of the relative atomic mass of the conjugates was carried out using Liquid Chromatography Mass Spectrometry (LCMS) on a LCMS-2010 EV (Shimadzu Corporation, Marne-la-Vallée, Paris, France) equipped with a diode array detector SPD-M20A, a column oven CT0-20AC and a DGU-20A3degasser.1 H NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer (Bruker, Wissenburg, Germany). .. All samples were prepared in deuterated dimethyl sulfoxide (DMSO-d 6 ) with a concentration of about 10 mM.

Thin Layer Chromatography:

Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
Article Snippet: .. Reactions were monitored by thin-layer chromatography (TLC) using Merck 60F254 silica gel plates or by Liquid Chromatography-Mass Spectrometry on Shimadzu LCMS-2020 with either Waters, XSelect HSS CYANO 3.6 µm column or Agilent XBD-C18 3.5 μm column; dimensions 2.1 × 20 mm and UV detector at 254 nM. .. Chromatography purification was performed on Biotage Isolera Four instrument using pre-filled KP-Sil (normal phase) and KP-C18-HS (reverse phase) SNAP cartridges with UV detection at 254 and 280 nm.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Development and Phytochemical Characterization of High Polyphenol Red Lettuce with Anti-Diabetic Properties
Article Snippet: .. Liquid Chromatography Mass Spectrometry (LCMS) Methanolic extract samples were separated and analyzed with a Shimadzu LCMS-2010A high performance liquid chromatography/electrospray ionization/single quadrupole mass spectrometer. .. The LCMS is equipped with two pumps (LC-10ADvp), controller (SCL-10Avp), auto-injector (SIL-10ADvp), column oven (CTO-10ACvp), photodiode array detector (SPD-M10Avp), and a single quadrupole analyzer.

Article Title: Synthetic Human β Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Article Snippet: .. The purity of the peptides was determined using HPLC (Shimadzu, Kyoto, Japan) and liquid chromatography-mass spectrometry (LC-MS, Shimadzu, Kyoto, Japan). ..

Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
Article Snippet: .. Reactions were monitored by thin-layer chromatography (TLC) using Merck 60F254 silica gel plates or by Liquid Chromatography-Mass Spectrometry on Shimadzu LCMS-2020 with either Waters, XSelect HSS CYANO 3.6 µm column or Agilent XBD-C18 3.5 μm column; dimensions 2.1 × 20 mm and UV detector at 254 nM. .. Chromatography purification was performed on Biotage Isolera Four instrument using pre-filled KP-Sil (normal phase) and KP-C18-HS (reverse phase) SNAP cartridges with UV detection at 254 and 280 nm.

Article Title: New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
Article Snippet: .. Chemical Characterization of TPC and the Novel TPC–Peptide Conjugates Verification of the relative atomic mass of the conjugates was carried out using Liquid Chromatography Mass Spectrometry (LCMS) on a LCMS-2010 EV (Shimadzu Corporation, Marne-la-Vallée, Paris, France) equipped with a diode array detector SPD-M20A, a column oven CT0-20AC and a DGU-20A3degasser.1 H NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer (Bruker, Wissenburg, Germany). .. All samples were prepared in deuterated dimethyl sulfoxide (DMSO-d 6 ) with a concentration of about 10 mM.

Article Title: Robustness of anaerobes exposed to cyanuric acid contaminated wastewater and achieving efficient removal via optimized co-digestion scheme
Article Snippet: .. CA concentration was determined using liquid chromatography-mass spectrometry (LCMS-2020, Shimadzu) with an APCI interface on negative polarity. .. The measurement conditions are as follows; i.e., shim-pack XR-ODS II column with a size of 3 × 100 mm was used; the mobile phase was 90% acetonitrile and 10% of 0.1 formic acid solution at a flow rate of 0.4 mL/min; the oven temperature was 30 °C, and the retention time was 5 min. Scanning electron microscopy (SEM) analysis for sludge samples was performed to examine the surface morphology change after long-term exposure to CA (Hitachi S-4500, Japan).

Liquid Chromatography:

Article Title: Development and Phytochemical Characterization of High Polyphenol Red Lettuce with Anti-Diabetic Properties
Article Snippet: .. Liquid Chromatography Mass Spectrometry (LCMS) Methanolic extract samples were separated and analyzed with a Shimadzu LCMS-2010A high performance liquid chromatography/electrospray ionization/single quadrupole mass spectrometer. .. The LCMS is equipped with two pumps (LC-10ADvp), controller (SCL-10Avp), auto-injector (SIL-10ADvp), column oven (CTO-10ACvp), photodiode array detector (SPD-M10Avp), and a single quadrupole analyzer.

Article Title: Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea
Article Snippet: .. Liquid Chromatography Mass Spectrometry The analyzes were conducted using a Shimadzu Prominence UFLC system (Shimadzu, Kyoto, Japan). ..

Article Title: New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
Article Snippet: .. Chemical Characterization of TPC and the Novel TPC–Peptide Conjugates Verification of the relative atomic mass of the conjugates was carried out using Liquid Chromatography Mass Spectrometry (LCMS) on a LCMS-2010 EV (Shimadzu Corporation, Marne-la-Vallée, Paris, France) equipped with a diode array detector SPD-M20A, a column oven CT0-20AC and a DGU-20A3degasser.1 H NMR spectra were recorded on a Bruker Avance 300 MHz spectrometer (Bruker, Wissenburg, Germany). .. All samples were prepared in deuterated dimethyl sulfoxide (DMSO-d 6 ) with a concentration of about 10 mM.

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    Shimadzu Corporation lc it tof ms
    <t>LC-IT-TOF</t> MS chromatograms of <t>Acori</t> Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).
    Lc It Tof Ms, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 3 article reviews
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    85
    Shimadzu Corporation mito chmac
    Intracellular accumulation of <t>Mito-ChM</t> in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM <t>Mito-ChMAc.</t> ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.
    Mito Chmac, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mito chmac/product/Shimadzu Corporation
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    Shimadzu Corporation direct lc ms ms analysis
    Enhanced product ion <t>(EPI)-MS</t> spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from <t>LC-MS</t> <t>analysis</t> of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.
    Direct Lc Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    direct lc ms ms analysis - by Bioz Stars, 2020-09
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    Shimadzu Corporation uplc pad analysis hplc pad analysis
    The <t>UPLC-PAD</t> chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).
    Uplc Pad Analysis Hplc Pad Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uplc pad analysis hplc pad analysis/product/Shimadzu Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uplc pad analysis hplc pad analysis - by Bioz Stars, 2020-09
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    Image Search Results


    LC-IT-TOF MS chromatograms of Acori Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Water Extract of Acori Graminei Rhizoma Attenuates Features of Rheumatoid Arthritis in DBA/1 Mice

    doi: 10.1155/2019/3637453

    Figure Lengend Snippet: LC-IT-TOF MS chromatograms of Acori Graminei Rhizoma. LC chromatogram (a) and total ion chromatogram in positive ion mode (b). β -asarone (1).

    Article Snippet: LC-IT-TOF-MS Conditions of Acori Graminei Rhizoma Extract WAG was analyzed by ESI-mass spectra which were obtained on a LC-IT-TOF mass spectrometer (Shimadzu, Japan).

    Techniques: Mass Spectrometry

    Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Intracellular accumulation of Mito-ChM in MCF-7, MDA-MB-231 and MCF-10A cells. ( A ) HPLC-EC chromatograms (dominant channels) of the mixture of standards (100 μM) of α-tocopherol and Mito-ChM, and of extracts from cells treated for 4 h with 10 μM Mito-ChM ( left panel ). Quantitative data on intracellular concentration of Mito-ChM after normalization to protein content ( right panel ). ( B ) Same as in panel A , but after a 4 h treatment with 10 μM Mito-ChM, medium was changed and cells incubated further for another 24 h in culture medium in the absence of Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (10 μM each). ( C ) Same as in panel A , but cells were treated for 48 h with 1 μM Mito-ChM. Chromatogram of standards represents a mixture of α-tocopherol and Mito-ChM (1 μM each). ( D ) Same as in panel A , but cells were treated for 4 h with 10 μM Mito-ChMAc. ( E ) HPLC-MS/MS chromatograms [MRM transitions: 679.1 → 515.0 for Mito-ChM ( upper traces ) and 721.1 → 415.0 for Mito-ChMAc ( lower traces )] of the mixture of standards (100 μM) of Mito-ChM and Mito-ChMAc, and of extracts from cells treated for 4 h with 10 μM Mito-ChMAc ( left panel ). Quantitative data on intracellular concentrations of Mito-ChM and MitoChMAc after normalization to protein content ( right panel ). ( F ) Intracellular levels of Mito-ChM in MDA-MB-231 cells incubated for 2 and 4 h with 10 μM Mito-ChM or Mito-ChMAc ( left panel ). Right panel shows similar data, but after 4 h incubation with the compounds, cells were incubated for 24 h in culture medium alone.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Multiple Displacement Amplification, High Performance Liquid Chromatography, Concentration Assay, Incubation, Mass Spectrometry

    Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Journal: BMC Cancer

    Article Title: Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death

    doi: 10.1186/1471-2407-13-285

    Figure Lengend Snippet: Synergistic cytotoxicity and anti-proliferative effects of Mito-ChM and 2-DG. ( A ) Representative pictures of the colonies formed. MCF-7 and MCF-10A cells were treated with 2-DG only ( upper ) or 2-DG in the presence of Mito-ChM (1 μM) ( lower ) for 4 h and the colonies formed were counted. ( B ) The survival fraction was calculated under the same conditions as in ( A ). ( C , D ) Cytotoxic effects of the combination of Mito-ChM and 2-DG. MCF-7 (main panels) and MCF-10A (inserts) cells were treated with 2-DG alone (at the indicated concentrations), 2-DG in the presence of 1 μM Mito-ChM ( C ) or 1 μM Mito-ChMAc ( D ) for 24 h and cell death was monitored in real time by Sytox Green staining. Data shown are the mean ± SEM. n = 4-6.

    Article Snippet: The simultaneous quantification of Mito-ChM and Mito-ChMAc in the extracts was performed using the UHPLC system (Shimadzu Nexera) coupled to an MS/MS detector (Shimadzu 8030).

    Techniques: Staining

    Enhanced product ion (EPI)-MS spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from LC-MS analysis of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.

    Journal: PLoS ONE

    Article Title: Broccoli Consumption Interacts with GSTM1 to Perturb Oncogenic Signalling Pathways in the Prostate

    doi: 10.1371/journal.pone.0002568

    Figure Lengend Snippet: Enhanced product ion (EPI)-MS spectra of the two insulin-SF conjugates. MS 2 product ion spectra of (A) 6.46 min and (B) 7.08 min retention time peaks from LC-MS analysis of human plasma incubated with bovine insulin and 50 µM SF for 4 h at 37°C. In (A) and (B) m/z 1183.9 corresponds to insulin-SF MH 5 5+ and in (A) m/z 235.0 corresponds to Gly-SF, the N-terminal amino acid of insulin A chain and in (B) m/z 325.2 corresponds to Phe-SF, the N-terminal amino acid of insulin B chain.

    Article Snippet: Direct LC-MS/MS analysis of peptides incubated with isothiocyanates The LC system used was a Shimadzu series 10AD VP (Shimadzu, http://www.shimadzu.com/ ).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Incubation

    The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

    doi: 10.1155/2015/264612

    Figure Lengend Snippet: The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients by UPLC-PAD analysis. The UPLC-PAD chromatograms of the Yejuhua capsule and its three ingredients. SFEC: supercritical-carbon dioxide fluid extraction of Chrysanthemi Indici Flos (a), PO: Patchouli Oil (b), ZTO: Zedoary Turmeric Oil (c), and YJH: Yejuhua capsule (d). The structures of authentic standard compounds used in the UPLC-PAD analysis (e).

    Article Snippet: Apparatus and UPLC-PAD Analysis HPLC-PAD analysis was carried out on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AT pump, a SIL-20AC automatic sampler, a CTO-20A thermostatic column compartment, and a Shimadzu LC-20A software (Shimadzu, Kyoto, Japan).

    Techniques:

    The GC-MS and UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule. The GC-MS fingerprinting chromatograms of 10 batches of Yejuhua capsule (a) and the UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule (b). YJH131-YJH1310: batch of Yejuhua capsule sample from number 1 to number 10.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Standardized Traditional Chinese Medicine Preparation Named Yejuhua Capsule Ameliorates Lipopolysaccharide-Induced Acute Lung Injury in Mice via Downregulating Toll-Like Receptor 4/Nuclear Factor-κB

    doi: 10.1155/2015/264612

    Figure Lengend Snippet: The GC-MS and UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule. The GC-MS fingerprinting chromatograms of 10 batches of Yejuhua capsule (a) and the UPLC-PAD fingerprinting chromatograms of 10 batches of Yejuhua capsule (b). YJH131-YJH1310: batch of Yejuhua capsule sample from number 1 to number 10.

    Article Snippet: Apparatus and UPLC-PAD Analysis HPLC-PAD analysis was carried out on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AT pump, a SIL-20AC automatic sampler, a CTO-20A thermostatic column compartment, and a Shimadzu LC-20A software (Shimadzu, Kyoto, Japan).

    Techniques: Gas Chromatography-Mass Spectrometry