liquid chromatography mass spectrometry lc ms  (SCIEX)

 
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    Structured Review

    SCIEX liquid chromatography mass spectrometry lc ms
    Liquid Chromatography Mass Spectrometry Lc Ms, supplied by SCIEX, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 7 article reviews
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    liquid chromatography mass spectrometry lc ms - by Bioz Stars, 2020-05
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    Liquid Chromatography with Mass Spectroscopy:

    Article Title: In Vitro and In Vivo Efficacy Studies of Lavender angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer
    Article Snippet: .. Liquid Chromatography/Mass Spectrometry (LC/MS) The presence of linalool and linalyl acetate in the essential oil was assessed by LC (Ekspert Ultra LC 100, Eksigent part of AB SCIEX)/MS (3200 Q TRAP LC/MS/MS system, AB SCIEX) with a reverse-phase column (Thermo Accucore C18). .. The chromatography conditions were a mobile phase A of ddH2 O with 0.1% formic acid and B of acetonitrile with 0.1% formic acid.

    Article Title: Urinary Metabolomics Identifies a Molecular Correlate of Interstitial Cystitis/Bladder Pain Syndrome in a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network Cohort
    Article Snippet: .. 2.4 Liquid Chromatography–Mass Spectrometry (LC–MS) The mass spectrometer used for this study was an AB Sciex API 4000 QTrap (AB Sciex, Foster City, CA) operated in the negative ion electrospray ionization (ESI) mode using the Turbo V ESI ion source. .. The mass spectrometer was coupled to a Shimadzu UFLC (Kyoto, Japan).

    Article Title: Soil-Applied Imidacloprid Translocates to Ornamental Flowers and Reduces Survival of Adult Coleomegilla maculata, Harmonia axyridis, and Hippodamia convergens Lady Beetles, and Larval Danaus plexippus and Vanessa cardui Butterflies
    Article Snippet: .. The samples were then analyzed by Liquid Chromatography-Mass Spectrometry LC/MS (PE Sciex API 3200 or 4000 Q-trap system) with variant solvent delivery system and Agilent Automatic Sample Injector. .. The operating conditions were a YMC-ODS-AM column, 5 μm particle size, 40°C, mobile phase A 0.1% acetic acid in water and mobile phase B 0.1% acetic acid in acetonitrile, flow rate 0.5 ml/min, and injection volume 15 μl.

    Article Title: Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway
    Article Snippet: .. Liquid Chromatography-Mass Spectrometry (LC-MS) The nonvolatile compounds in the ethanolic extract of A. sessilis 's stem were identified using the AB Sciex 5500 QTrap LC-MS/MS with Agilent Technologies 1290 series UHPLC. .. The separation was performed using the column Phenomenex Synergi Fusion 100 mm × 2.1 mm × 3 μ m held at 25°C with the enhanced MS (EMS) parameters as follows: nebulizer gas (N2) temperature 500°C, flow rate 10 L/min, pressure 40 psi, and capillary voltage 4500 V, with the scanning range of 100–1000 m /z for full scan and 50–1000 m /z for MS/MS scan.

    Article Title: Transcriptome profiling reveals the response process of tomato carrying Cf-19 and Cladosporium fulvum interaction
    Article Snippet: .. The SA and JA concentrations were subsequently measured using liquid chromatography-mass spectrometry (LC-MS) according to the manufacturer’s instructions (AB Sciex QTRAP 5500, USA). ..

    Article Title: Reversible Cysteine Protease Inhibitors Show Promise for a Chagas Disease Cure
    Article Snippet: .. All compounds were analyzed by quantitative liquid chromatography-mass spectrometry (LC-MS) by using a SCIEX 2000 apparatus (Toronto, Canada) with positive ion electron spray ionization. .. LC separation was achieved by using a Luna C18 3-μm (50- by 2-mm) LC column (Phenomenex) with a mobile-phase gradient consisting of acetonitrile and 0.1% formic acid.

    Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation
    Article Snippet: .. Electrospray ionization mass spectrometry (ES-MS) and liquid chromatography mass spectrometry (LC-MS) were conducted on a Perkin-Elmer-Sciex API 3000 mass spectrometer (Applied Biosystems/MDS Sciex, Toronto, Canada). .. Solvents used for ES-MS and LC-MS consisted of solvent A: 0.1% acetic acid (AcOH) in water and solvent B: 90% ACN/10% water/0.1% AcOH.

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. To detect whether TPSA was methylated by Rv0558 or Rv0560c, methylation reaction was incubated at 37°C for 14 h. The methylated product was detected using liquid chromatography-mass spectrometry (LC-MS) (AB Sciex 5500 Q-trap). ..

    Chromatography:

    Article Title: In Vitro and In Vivo Efficacy Studies of Lavender angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer
    Article Snippet: .. Liquid Chromatography/Mass Spectrometry (LC/MS) The presence of linalool and linalyl acetate in the essential oil was assessed by LC (Ekspert Ultra LC 100, Eksigent part of AB SCIEX)/MS (3200 Q TRAP LC/MS/MS system, AB SCIEX) with a reverse-phase column (Thermo Accucore C18). .. The chromatography conditions were a mobile phase A of ddH2 O with 0.1% formic acid and B of acetonitrile with 0.1% formic acid.

    Article Title: Urinary Metabolomics Identifies a Molecular Correlate of Interstitial Cystitis/Bladder Pain Syndrome in a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network Cohort
    Article Snippet: .. 2.4 Liquid Chromatography–Mass Spectrometry (LC–MS) The mass spectrometer used for this study was an AB Sciex API 4000 QTrap (AB Sciex, Foster City, CA) operated in the negative ion electrospray ionization (ESI) mode using the Turbo V ESI ion source. .. The mass spectrometer was coupled to a Shimadzu UFLC (Kyoto, Japan).

    Article Title: Soil-Applied Imidacloprid Translocates to Ornamental Flowers and Reduces Survival of Adult Coleomegilla maculata, Harmonia axyridis, and Hippodamia convergens Lady Beetles, and Larval Danaus plexippus and Vanessa cardui Butterflies
    Article Snippet: .. The samples were then analyzed by Liquid Chromatography-Mass Spectrometry LC/MS (PE Sciex API 3200 or 4000 Q-trap system) with variant solvent delivery system and Agilent Automatic Sample Injector. .. The operating conditions were a YMC-ODS-AM column, 5 μm particle size, 40°C, mobile phase A 0.1% acetic acid in water and mobile phase B 0.1% acetic acid in acetonitrile, flow rate 0.5 ml/min, and injection volume 15 μl.

    Article Title: Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway
    Article Snippet: .. Liquid Chromatography-Mass Spectrometry (LC-MS) The nonvolatile compounds in the ethanolic extract of A. sessilis 's stem were identified using the AB Sciex 5500 QTrap LC-MS/MS with Agilent Technologies 1290 series UHPLC. .. The separation was performed using the column Phenomenex Synergi Fusion 100 mm × 2.1 mm × 3 μ m held at 25°C with the enhanced MS (EMS) parameters as follows: nebulizer gas (N2) temperature 500°C, flow rate 10 L/min, pressure 40 psi, and capillary voltage 4500 V, with the scanning range of 100–1000 m /z for full scan and 50–1000 m /z for MS/MS scan.

    Article Title: Transcriptome profiling reveals the response process of tomato carrying Cf-19 and Cladosporium fulvum interaction
    Article Snippet: .. The SA and JA concentrations were subsequently measured using liquid chromatography-mass spectrometry (LC-MS) according to the manufacturer’s instructions (AB Sciex QTRAP 5500, USA). ..

    Article Title: Reversible Cysteine Protease Inhibitors Show Promise for a Chagas Disease Cure
    Article Snippet: .. All compounds were analyzed by quantitative liquid chromatography-mass spectrometry (LC-MS) by using a SCIEX 2000 apparatus (Toronto, Canada) with positive ion electron spray ionization. .. LC separation was achieved by using a Luna C18 3-μm (50- by 2-mm) LC column (Phenomenex) with a mobile-phase gradient consisting of acetonitrile and 0.1% formic acid.

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. To detect whether TPSA was methylated by Rv0558 or Rv0560c, methylation reaction was incubated at 37°C for 14 h. The methylated product was detected using liquid chromatography-mass spectrometry (LC-MS) (AB Sciex 5500 Q-trap). ..

    Methylation:

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. To detect whether TPSA was methylated by Rv0558 or Rv0560c, methylation reaction was incubated at 37°C for 14 h. The methylated product was detected using liquid chromatography-mass spectrometry (LC-MS) (AB Sciex 5500 Q-trap). ..

    Incubation:

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. To detect whether TPSA was methylated by Rv0558 or Rv0560c, methylation reaction was incubated at 37°C for 14 h. The methylated product was detected using liquid chromatography-mass spectrometry (LC-MS) (AB Sciex 5500 Q-trap). ..

    Mass Spectrometry:

    Article Title: Urinary Metabolomics Identifies a Molecular Correlate of Interstitial Cystitis/Bladder Pain Syndrome in a Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network Cohort
    Article Snippet: .. 2.4 Liquid Chromatography–Mass Spectrometry (LC–MS) The mass spectrometer used for this study was an AB Sciex API 4000 QTrap (AB Sciex, Foster City, CA) operated in the negative ion electrospray ionization (ESI) mode using the Turbo V ESI ion source. .. The mass spectrometer was coupled to a Shimadzu UFLC (Kyoto, Japan).

    Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation
    Article Snippet: .. Electrospray ionization mass spectrometry (ES-MS) and liquid chromatography mass spectrometry (LC-MS) were conducted on a Perkin-Elmer-Sciex API 3000 mass spectrometer (Applied Biosystems/MDS Sciex, Toronto, Canada). .. Solvents used for ES-MS and LC-MS consisted of solvent A: 0.1% acetic acid (AcOH) in water and solvent B: 90% ACN/10% water/0.1% AcOH.

    Variant Assay:

    Article Title: Soil-Applied Imidacloprid Translocates to Ornamental Flowers and Reduces Survival of Adult Coleomegilla maculata, Harmonia axyridis, and Hippodamia convergens Lady Beetles, and Larval Danaus plexippus and Vanessa cardui Butterflies
    Article Snippet: .. The samples were then analyzed by Liquid Chromatography-Mass Spectrometry LC/MS (PE Sciex API 3200 or 4000 Q-trap system) with variant solvent delivery system and Agilent Automatic Sample Injector. .. The operating conditions were a YMC-ODS-AM column, 5 μm particle size, 40°C, mobile phase A 0.1% acetic acid in water and mobile phase B 0.1% acetic acid in acetonitrile, flow rate 0.5 ml/min, and injection volume 15 μl.

    Liquid Chromatography:

    Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation
    Article Snippet: .. Electrospray ionization mass spectrometry (ES-MS) and liquid chromatography mass spectrometry (LC-MS) were conducted on a Perkin-Elmer-Sciex API 3000 mass spectrometer (Applied Biosystems/MDS Sciex, Toronto, Canada). .. Solvents used for ES-MS and LC-MS consisted of solvent A: 0.1% acetic acid (AcOH) in water and solvent B: 90% ACN/10% water/0.1% AcOH.

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    SCIEX quantitative lc ms ms results
    Correlation of <t>results</t> obtained by both MNP-bsELISA and <t>LC-MS/MS</t> for zearalenone detection in natural cereal and feed samples.
    Quantitative Lc Ms Ms Results, supplied by SCIEX, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 2 article reviews
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    SCIEX normal phase lc ms ms
    Hepatic TG composition in genetically modified PNPLA3 mice. A , the number of carbons ( left ) and number of double bonds ( right ) in hepatic TGs of IM-ki ( red ), SA-ki ( blue ), and KO ( black ) mice relative to their WT counterparts. Male mice were fed a HSD for 4 weeks ( n = 6–8/group, 14 weeks old) and then sacrificed after 4 h of refeeding. Hepatic TGs were analyzed by direct infusion lipidomics. TGs were grouped based on number of FA carbons ( left ) or double bonds ( right ), as determined by the <t>MS/MS</t> signal from untargeted lipidomics analysis. Values were normalized to the total lipid signal and expressed relative to the corresponding value in WT mice. The data are plotted on a <t>log-normal</t> scale. B , selected TGs were measured by <t>LC-MS/MS</t> analysis. Shown are representative examples of abundant TGs ( top ) and vLCPUFA-containing TGs ( bottom ) in livers of PNPLA3 genetically modified mice. Mass pairs for identification of abundant TG were 876.7 and 577.6, 848.7 and 577.6, and 874.7 and 577.6 Da for the TGs shown left to right , respectively, and 898.8 and 577.7, 924.8 and 577.7, and 922.8 and 577.7 for the vLCPUFA-containing TGs shown left to right , respectively. Underlined FAs indicate the neutral loss of the measured species. Values were normalized to the sum of all measured TG signals. Liver TG analysis was repeated twice with similar results. Error bars , S.D. *, p
    Normal Phase Lc Ms Ms, supplied by SCIEX, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal phase lc ms ms/product/SCIEX
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    SCIEX lc ms ms based lipidomics
    Dietary DHA regulates tissue levels and PMN production of the eicosanoid SPM LXA 4 in healthy mice. Endogenous LXA 4 and LTB 4 tissue levels in plasma, cornea, lacrimal gland and draining lymph nodes of healthy mice (males females) on a DHA-enriched or DHA-deficient diet was quantified by LC/MS/MS-based <t>lipidomics.</t> A) LXA 4 and LTB 4 plasma levels (n=4). B) LXA 4 tissue levels (n=6) in corneas, lacrimal glands and draining lymph nodes. C) PMN from draining lymph nodes were isolated from healthy males and females on the DHA-enriched or DHA-deficient diets. PMN were activated with calcium ionophore at 37 ° C for 15 minutes and endogenous LXA 4 and LTB 4 formation quantified by LC/MS/MS-based lipidomics (n=5, pg/1×10 5 PMN). D) qPCR analysis of 5-LOX and 15-LOX RNA expression in lymph node PMN (n=4). (A-D) Values represent the mean ± SEM. * P
    Lc Ms Ms Based Lipidomics, supplied by SCIEX, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms ms based lipidomics/product/SCIEX
    Average 93 stars, based on 4 article reviews
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    86
    SCIEX lc ms ms amplifex diene
    Correlation of the <t>Amplifex</t> diene method of derivatization with the RIA (circles) and PTAD (triangles) methods of derivatization. The Amplifex diene method correlated well with both methods, but less so at the low concentration standards where the PTAD method was not as sensitive at the Amplifex diene method.
    Lc Ms Ms Amplifex Diene, supplied by SCIEX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Correlation of results obtained by both MNP-bsELISA and LC-MS/MS for zearalenone detection in natural cereal and feed samples.

    Journal: Toxins

    Article Title: A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples

    doi: 10.3390/toxins7104216

    Figure Lengend Snippet: Correlation of results obtained by both MNP-bsELISA and LC-MS/MS for zearalenone detection in natural cereal and feed samples.

    Article Snippet: Quantitative LC-MS/MS results were analyzed using Analyst software (AB SCIEX, Framingham, MA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Hepatic TG composition in genetically modified PNPLA3 mice. A , the number of carbons ( left ) and number of double bonds ( right ) in hepatic TGs of IM-ki ( red ), SA-ki ( blue ), and KO ( black ) mice relative to their WT counterparts. Male mice were fed a HSD for 4 weeks ( n = 6–8/group, 14 weeks old) and then sacrificed after 4 h of refeeding. Hepatic TGs were analyzed by direct infusion lipidomics. TGs were grouped based on number of FA carbons ( left ) or double bonds ( right ), as determined by the MS/MS signal from untargeted lipidomics analysis. Values were normalized to the total lipid signal and expressed relative to the corresponding value in WT mice. The data are plotted on a log-normal scale. B , selected TGs were measured by LC-MS/MS analysis. Shown are representative examples of abundant TGs ( top ) and vLCPUFA-containing TGs ( bottom ) in livers of PNPLA3 genetically modified mice. Mass pairs for identification of abundant TG were 876.7 and 577.6, 848.7 and 577.6, and 874.7 and 577.6 Da for the TGs shown left to right , respectively, and 898.8 and 577.7, 924.8 and 577.7, and 922.8 and 577.7 for the vLCPUFA-containing TGs shown left to right , respectively. Underlined FAs indicate the neutral loss of the measured species. Values were normalized to the sum of all measured TG signals. Liver TG analysis was repeated twice with similar results. Error bars , S.D. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Patatin-like phospholipase domain–containing protein 3 promotes transfers of essential fatty acids from triglycerides to phospholipids in hepatic lipid droplets

    doi: 10.1074/jbc.RA118.002333

    Figure Lengend Snippet: Hepatic TG composition in genetically modified PNPLA3 mice. A , the number of carbons ( left ) and number of double bonds ( right ) in hepatic TGs of IM-ki ( red ), SA-ki ( blue ), and KO ( black ) mice relative to their WT counterparts. Male mice were fed a HSD for 4 weeks ( n = 6–8/group, 14 weeks old) and then sacrificed after 4 h of refeeding. Hepatic TGs were analyzed by direct infusion lipidomics. TGs were grouped based on number of FA carbons ( left ) or double bonds ( right ), as determined by the MS/MS signal from untargeted lipidomics analysis. Values were normalized to the total lipid signal and expressed relative to the corresponding value in WT mice. The data are plotted on a log-normal scale. B , selected TGs were measured by LC-MS/MS analysis. Shown are representative examples of abundant TGs ( top ) and vLCPUFA-containing TGs ( bottom ) in livers of PNPLA3 genetically modified mice. Mass pairs for identification of abundant TG were 876.7 and 577.6, 848.7 and 577.6, and 874.7 and 577.6 Da for the TGs shown left to right , respectively, and 898.8 and 577.7, 924.8 and 577.7, and 922.8 and 577.7 for the vLCPUFA-containing TGs shown left to right , respectively. Underlined FAs indicate the neutral loss of the measured species. Values were normalized to the sum of all measured TG signals. Liver TG analysis was repeated twice with similar results. Error bars , S.D. *, p

    Article Snippet: Targeted analysis of PC, PE, PS, PG, and BMPs was performed by normal-phase LC-MS/MS, adapted from Hutchins et al. , with a Sciex 5000 triple quadrapole MS in line with a Shimadzu LC20 HPLC.

    Techniques: Genetically Modified, Mouse Assay, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Dietary DHA regulates tissue levels and PMN production of the eicosanoid SPM LXA 4 in healthy mice. Endogenous LXA 4 and LTB 4 tissue levels in plasma, cornea, lacrimal gland and draining lymph nodes of healthy mice (males females) on a DHA-enriched or DHA-deficient diet was quantified by LC/MS/MS-based lipidomics. A) LXA 4 and LTB 4 plasma levels (n=4). B) LXA 4 tissue levels (n=6) in corneas, lacrimal glands and draining lymph nodes. C) PMN from draining lymph nodes were isolated from healthy males and females on the DHA-enriched or DHA-deficient diets. PMN were activated with calcium ionophore at 37 ° C for 15 minutes and endogenous LXA 4 and LTB 4 formation quantified by LC/MS/MS-based lipidomics (n=5, pg/1×10 5 PMN). D) qPCR analysis of 5-LOX and 15-LOX RNA expression in lymph node PMN (n=4). (A-D) Values represent the mean ± SEM. * P

    Journal: Mucosal immunology

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease

    doi: 10.1038/s41385-018-0070-z

    Figure Lengend Snippet: Dietary DHA regulates tissue levels and PMN production of the eicosanoid SPM LXA 4 in healthy mice. Endogenous LXA 4 and LTB 4 tissue levels in plasma, cornea, lacrimal gland and draining lymph nodes of healthy mice (males females) on a DHA-enriched or DHA-deficient diet was quantified by LC/MS/MS-based lipidomics. A) LXA 4 and LTB 4 plasma levels (n=4). B) LXA 4 tissue levels (n=6) in corneas, lacrimal glands and draining lymph nodes. C) PMN from draining lymph nodes were isolated from healthy males and females on the DHA-enriched or DHA-deficient diets. PMN were activated with calcium ionophore at 37 ° C for 15 minutes and endogenous LXA 4 and LTB 4 formation quantified by LC/MS/MS-based lipidomics (n=5, pg/1×10 5 PMN). D) qPCR analysis of 5-LOX and 15-LOX RNA expression in lymph node PMN (n=4). (A-D) Values represent the mean ± SEM. * P

    Article Snippet: Lipid mediators were identified and quantified by LC/MS/MS-based lipidomics using a AB Sciex QTRAP 3200 MS as previously described , .

    Techniques: Mouse Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Isolation, Real-time Polymerase Chain Reaction, RNA Expression

    Acute DHA treatment rescues females from amplified T cell responses and amplifies endogenous LXA 4 formation in tissues. Female mice on the DHA-deficient diet (4 weeks) were treated locally and systemically with DHA or saline alone starting at Day 1 of desiccating stress. After 10 days of desiccating stress draining lymph nodes were collected for analysis by flow cytometry. A) Bar graph PMN (CD45 high Ly6g high ) in draining lymph nodes (n=3, with pooled cervical lymph nodes from 1 mouse for each group). B) Frequencies of T reg cells (CD4 + Foxp3 + ),T H 1 cells (CD4 + IFNγ + ) and T H 17 (CD4 + IL17 + ) cells among absolute number of lymphocytes. C) LXA 4 levels were measured by LC/MS/MS-based lipidomics in plasma (n=4), cornea (n=5), lacrimal gland (n=5) and draining lymph nodes (n=5). D) qPCR analysis of 5-LOX, 15-LOX and LXA 4 receptor (ALX) RNA expression in draining lymph nodes. Values represent the mean ± SEM. * P

    Journal: Mucosal immunology

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease

    doi: 10.1038/s41385-018-0070-z

    Figure Lengend Snippet: Acute DHA treatment rescues females from amplified T cell responses and amplifies endogenous LXA 4 formation in tissues. Female mice on the DHA-deficient diet (4 weeks) were treated locally and systemically with DHA or saline alone starting at Day 1 of desiccating stress. After 10 days of desiccating stress draining lymph nodes were collected for analysis by flow cytometry. A) Bar graph PMN (CD45 high Ly6g high ) in draining lymph nodes (n=3, with pooled cervical lymph nodes from 1 mouse for each group). B) Frequencies of T reg cells (CD4 + Foxp3 + ),T H 1 cells (CD4 + IFNγ + ) and T H 17 (CD4 + IL17 + ) cells among absolute number of lymphocytes. C) LXA 4 levels were measured by LC/MS/MS-based lipidomics in plasma (n=4), cornea (n=5), lacrimal gland (n=5) and draining lymph nodes (n=5). D) qPCR analysis of 5-LOX, 15-LOX and LXA 4 receptor (ALX) RNA expression in draining lymph nodes. Values represent the mean ± SEM. * P

    Article Snippet: Lipid mediators were identified and quantified by LC/MS/MS-based lipidomics using a AB Sciex QTRAP 3200 MS as previously described , .

    Techniques: Amplification, Mouse Assay, Flow Cytometry, Cytometry, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Real-time Polymerase Chain Reaction, RNA Expression

    Correlation of the Amplifex diene method of derivatization with the RIA (circles) and PTAD (triangles) methods of derivatization. The Amplifex diene method correlated well with both methods, but less so at the low concentration standards where the PTAD method was not as sensitive at the Amplifex diene method.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Development of a Sensitive LC/MS/MS Method for Vitamin D Metabolites: 1,25 Dihydroxyvitamin D23 Measurement Using a Novel Derivatization Agent

    doi: 10.1016/j.jchromb.2014.01.045

    Figure Lengend Snippet: Correlation of the Amplifex diene method of derivatization with the RIA (circles) and PTAD (triangles) methods of derivatization. The Amplifex diene method correlated well with both methods, but less so at the low concentration standards where the PTAD method was not as sensitive at the Amplifex diene method.

    Article Snippet: Recently, a new reagent for derivatizing 1,25-(OH)2 -D2 and -D3 was developed specifically for LC/MS/MS: Amplifex diene (AB SCIEX).

    Techniques: Concentration Assay

    Chemical structure of Amplifex diene reagent used to derivatize 1,25-(OH) 2 -D 3 and -D 2 . Amplifex improves ionization and enhances sensitivity for molecules that have a cis-diene system.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Development of a Sensitive LC/MS/MS Method for Vitamin D Metabolites: 1,25 Dihydroxyvitamin D23 Measurement Using a Novel Derivatization Agent

    doi: 10.1016/j.jchromb.2014.01.045

    Figure Lengend Snippet: Chemical structure of Amplifex diene reagent used to derivatize 1,25-(OH) 2 -D 3 and -D 2 . Amplifex improves ionization and enhances sensitivity for molecules that have a cis-diene system.

    Article Snippet: Recently, a new reagent for derivatizing 1,25-(OH)2 -D2 and -D3 was developed specifically for LC/MS/MS: Amplifex diene (AB SCIEX).

    Techniques: