liquid chromatography mass spectrometry lc ms  (Hitachi Ltd)

 
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    Structured Review

    Hitachi Ltd liquid chromatography mass spectrometry lc ms
    Liquid Chromatography Mass Spectrometry Lc Ms, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    liquid chromatography mass spectrometry lc ms - by Bioz Stars, 2020-05
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    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals
    Article Snippet: .. The CO2 gas produced as a result of the reaction between phosphoric acid and purified calcite was analyzed quantitatively using liquid chromatography-mass spectrometry (LC/MS) (Hitachi RMI-2, Tokyo, Japan) to determine the ratio of 13 C atoms to total carbon (12 C plus 13 C) atoms (atom%) in the calcites ( ). .. A purified calcite formed peripheral to the parent colony was placed on a glass slide and dissolved in 2 μl of 1 N HCl ( ).

    Article Title: Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2
    Article Snippet: .. Chemical structures of intermediates were explored by Liquid Chromatography Mass Spectrometry (LC-MS) using a Hitachi Elite LaChrom L-2130 liquid chromatograph coupled to a Bruker Esquire 6000 MS. .. The MS detector is equipped with an electro spray ionization in positive and negative mode and a quadrupole analyzer.

    Chromatography:

    Article Title: Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals
    Article Snippet: .. The CO2 gas produced as a result of the reaction between phosphoric acid and purified calcite was analyzed quantitatively using liquid chromatography-mass spectrometry (LC/MS) (Hitachi RMI-2, Tokyo, Japan) to determine the ratio of 13 C atoms to total carbon (12 C plus 13 C) atoms (atom%) in the calcites ( ). .. A purified calcite formed peripheral to the parent colony was placed on a glass slide and dissolved in 2 μl of 1 N HCl ( ).

    Purification:

    Article Title: Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals
    Article Snippet: .. The CO2 gas produced as a result of the reaction between phosphoric acid and purified calcite was analyzed quantitatively using liquid chromatography-mass spectrometry (LC/MS) (Hitachi RMI-2, Tokyo, Japan) to determine the ratio of 13 C atoms to total carbon (12 C plus 13 C) atoms (atom%) in the calcites ( ). .. A purified calcite formed peripheral to the parent colony was placed on a glass slide and dissolved in 2 μl of 1 N HCl ( ).

    Produced:

    Article Title: Geobacillus thermoglucosidasius Endospores Function as Nuclei for the Formation of Single Calcite Crystals
    Article Snippet: .. The CO2 gas produced as a result of the reaction between phosphoric acid and purified calcite was analyzed quantitatively using liquid chromatography-mass spectrometry (LC/MS) (Hitachi RMI-2, Tokyo, Japan) to determine the ratio of 13 C atoms to total carbon (12 C plus 13 C) atoms (atom%) in the calcites ( ). .. A purified calcite formed peripheral to the parent colony was placed on a glass slide and dissolved in 2 μl of 1 N HCl ( ).

    Mass Spectrometry:

    Article Title: Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2
    Article Snippet: .. Chemical structures of intermediates were explored by Liquid Chromatography Mass Spectrometry (LC-MS) using a Hitachi Elite LaChrom L-2130 liquid chromatograph coupled to a Bruker Esquire 6000 MS. .. The MS detector is equipped with an electro spray ionization in positive and negative mode and a quadrupole analyzer.

    Liquid Chromatography:

    Article Title: Biochemical, Transcriptional and Translational Evidences of the Phenol-meta-Degradation Pathway by the Hyperthermophilic Sulfolobus solfataricus 98/2
    Article Snippet: .. Chemical structures of intermediates were explored by Liquid Chromatography Mass Spectrometry (LC-MS) using a Hitachi Elite LaChrom L-2130 liquid chromatograph coupled to a Bruker Esquire 6000 MS. .. The MS detector is equipped with an electro spray ionization in positive and negative mode and a quadrupole analyzer.

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    Hitachi Ltd hϵdguo analysis lc apci ms analyses
    Amount of <t>HϵdGuo</t> in CESS cells. NT , no treatment; CA , treated with 1.0 μ m A23187; CA+VC , treated with 1.0 μ m A23187 and 1.0 m m vitamin C; CA+MK , treated with 1.0 μ m A23187 and 1.0 μ m MK886; CA+ASP , treated with 1.0 μ m A23187 and 200.0 μ m aspirin. Analyses were performed by stable isotope dilution <t>LC-APCI/MRM/MS</t> of PFB derivatives. Determinations were conducted in triplicate (means ± S.D.). **, p
    Hϵdguo Analysis Lc Apci Ms Analyses, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd hplc esi qtof conditions lc ms data
    Bar charts of normalised peak areas of six intermediates of arginine biosynthesis after <t>HPLC-ESI-qTOF</t> analysis. C. glutamicum ATCC 13032 (WT) and seven double deletion mutants were cultivated with l -arginine until exponential phase. Then l -arginine was removed and cells were further incubated to accumulate intracellular metabolites. The boxes in each diagram indicate the respective intracellular compound and its mass-to-charge ratio. Peak detection and integration was performed on base peak chromatograms (BPC) of m/z-values of [M + H] + ions. Values that are significantly different from the wildtype level (Student’s T-test p
    Hplc Esi Qtof Conditions Lc Ms Data, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd liquid chromatography lc mrm mass spectrometry ms
    Amounts of intracellular <t>TOG</t> in R15LO cells with increasing concentrations of arachidonic acid in the media. Analyses were conducted in duplicates by stable isotope dilution LC- electrospray <t>ionization/MRM/MS</t> method. (Mean ± SD, n = 2).
    Liquid Chromatography Lc Mrm Mass Spectrometry Ms, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hitachi Ltd lc uv ms
    Amounts of intracellular <t>TOG</t> in R15LO cells with increasing concentrations of arachidonic acid in the media. Analyses were conducted in duplicates by stable isotope dilution LC- electrospray <t>ionization/MRM/MS</t> method. (Mean ± SD, n = 2).
    Lc Uv Ms, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amount of HϵdGuo in CESS cells. NT , no treatment; CA , treated with 1.0 μ m A23187; CA+VC , treated with 1.0 μ m A23187 and 1.0 m m vitamin C; CA+MK , treated with 1.0 μ m A23187 and 1.0 μ m MK886; CA+ASP , treated with 1.0 μ m A23187 and 200.0 μ m aspirin. Analyses were performed by stable isotope dilution LC-APCI/MRM/MS of PFB derivatives. Determinations were conducted in triplicate (means ± S.D.). **, p

    Journal: The Journal of Biological Chemistry

    Article Title: 5-Lipoxygenase-mediated Endogenous DNA Damage *

    doi: 10.1074/jbc.M109.011841

    Figure Lengend Snippet: Amount of HϵdGuo in CESS cells. NT , no treatment; CA , treated with 1.0 μ m A23187; CA+VC , treated with 1.0 μ m A23187 and 1.0 m m vitamin C; CA+MK , treated with 1.0 μ m A23187 and 1.0 μ m MK886; CA+ASP , treated with 1.0 μ m A23187 and 200.0 μ m aspirin. Analyses were performed by stable isotope dilution LC-APCI/MRM/MS of PFB derivatives. Determinations were conducted in triplicate (means ± S.D.). **, p

    Article Snippet: LC Conditions for HϵdGuo Analysis LC-APCI/MS analyses were conducted using gradient system 3 on Hitachi l -2200 Autosampler equipped with Hitachi l -2130 Pump (Hitachi, San Jose, CA).

    Techniques: Isotope Dilution, Mass Spectrometry

    Representative of LC-APCI/MRM/MS chromatograms of HϵdGuo-PFB ( m / z 584 → m / z 468) and 15 N 5 -HϵdGuo-PFB ( m / z 589 → m / z 473). A , scheme of derivatization of HϵdGuo-PFB. B , chromatograms from unstimulated CESS cells. C , chromatograms from CESS cells treated with 1.0 μ m A23187 . D , chromatograms from CESS cells treated with 1.0 μ m A23187 and 1.0 m m vitamin C . E , chromatograms from CESS cells treated with 1.0 μ m A23187 and 1.0 μ m MK886 . F , chromatograms from CESS cells treated with 1.0 μ m A23187 and 200.0 μ m aspirin.

    Journal: The Journal of Biological Chemistry

    Article Title: 5-Lipoxygenase-mediated Endogenous DNA Damage *

    doi: 10.1074/jbc.M109.011841

    Figure Lengend Snippet: Representative of LC-APCI/MRM/MS chromatograms of HϵdGuo-PFB ( m / z 584 → m / z 468) and 15 N 5 -HϵdGuo-PFB ( m / z 589 → m / z 473). A , scheme of derivatization of HϵdGuo-PFB. B , chromatograms from unstimulated CESS cells. C , chromatograms from CESS cells treated with 1.0 μ m A23187 . D , chromatograms from CESS cells treated with 1.0 μ m A23187 and 1.0 m m vitamin C . E , chromatograms from CESS cells treated with 1.0 μ m A23187 and 1.0 μ m MK886 . F , chromatograms from CESS cells treated with 1.0 μ m A23187 and 200.0 μ m aspirin.

    Article Snippet: LC Conditions for HϵdGuo Analysis LC-APCI/MS analyses were conducted using gradient system 3 on Hitachi l -2200 Autosampler equipped with Hitachi l -2130 Pump (Hitachi, San Jose, CA).

    Techniques: Mass Spectrometry

    Bar charts of normalised peak areas of six intermediates of arginine biosynthesis after HPLC-ESI-qTOF analysis. C. glutamicum ATCC 13032 (WT) and seven double deletion mutants were cultivated with l -arginine until exponential phase. Then l -arginine was removed and cells were further incubated to accumulate intracellular metabolites. The boxes in each diagram indicate the respective intracellular compound and its mass-to-charge ratio. Peak detection and integration was performed on base peak chromatograms (BPC) of m/z-values of [M + H] + ions. Values that are significantly different from the wildtype level (Student’s T-test p

    Journal: BMC Genomics

    Article Title: A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum

    doi: 10.1186/1471-2164-14-713

    Figure Lengend Snippet: Bar charts of normalised peak areas of six intermediates of arginine biosynthesis after HPLC-ESI-qTOF analysis. C. glutamicum ATCC 13032 (WT) and seven double deletion mutants were cultivated with l -arginine until exponential phase. Then l -arginine was removed and cells were further incubated to accumulate intracellular metabolites. The boxes in each diagram indicate the respective intracellular compound and its mass-to-charge ratio. Peak detection and integration was performed on base peak chromatograms (BPC) of m/z-values of [M + H] + ions. Values that are significantly different from the wildtype level (Student’s T-test p

    Article Snippet: HPLC-ESI-QTOF conditions LC-MS data were obtained using a LaChromUltra (Hitachi Europe, UK) HPLC system coupled to a microTOF-Q hybrid quadrupole/time-of-flight mass spectrometer (Bruker Daltonics, Bremen, Germany), equipped with an electrospray ionisation (ESI) source.

    Techniques: High Performance Liquid Chromatography, Incubation

    Amounts of intracellular TOG in R15LO cells with increasing concentrations of arachidonic acid in the media. Analyses were conducted in duplicates by stable isotope dilution LC- electrospray ionization/MRM/MS method. (Mean ± SD, n = 2).

    Journal:

    Article Title: A 4-Oxo-2(E)-Nonenal-Derived Glutathione-Adduct from 15-Lipoxygenase-1-Mediated Oxidation of Cytosolic and Esterified Arachidonic Acid §

    doi: 10.1016/j.freeradbiomed.2009.06.035

    Figure Lengend Snippet: Amounts of intracellular TOG in R15LO cells with increasing concentrations of arachidonic acid in the media. Analyses were conducted in duplicates by stable isotope dilution LC- electrospray ionization/MRM/MS method. (Mean ± SD, n = 2).

    Article Snippet: Liquid chromatography (LC)-MRM/mass spectrometry (MS) for quantitative TOG analysis was conducted using gradient systems 1 and 2 on a Hitachi L-2130 Intelligent Pump (Hitachi, San Jose, CA).

    Techniques: Isotope Dilution, Mass Spectrometry

    LC- electrospray ionization/MRM/MS analysis of TOG and the [ 13 C9]-TOG in R15LO cells with 20 μM [ 13 C 20 ]-arachidonic acid treatment. (A) TOG, (B) [ 2 H 3 ]-TOG, (C) [ 13 C 9 ]-TOG. Ion chromatograms for TOG ( m/z 426 → m/z 280), [ 2 H 3 ]-TOG ( m/z 429

    Journal:

    Article Title: A 4-Oxo-2(E)-Nonenal-Derived Glutathione-Adduct from 15-Lipoxygenase-1-Mediated Oxidation of Cytosolic and Esterified Arachidonic Acid §

    doi: 10.1016/j.freeradbiomed.2009.06.035

    Figure Lengend Snippet: LC- electrospray ionization/MRM/MS analysis of TOG and the [ 13 C9]-TOG in R15LO cells with 20 μM [ 13 C 20 ]-arachidonic acid treatment. (A) TOG, (B) [ 2 H 3 ]-TOG, (C) [ 13 C 9 ]-TOG. Ion chromatograms for TOG ( m/z 426 → m/z 280), [ 2 H 3 ]-TOG ( m/z 429

    Article Snippet: Liquid chromatography (LC)-MRM/mass spectrometry (MS) for quantitative TOG analysis was conducted using gradient systems 1 and 2 on a Hitachi L-2130 Intelligent Pump (Hitachi, San Jose, CA).

    Techniques: Mass Spectrometry

    LC-electrospray ionization/MRM/MS analysis of TOG and its corresponding internal standard [ 2 H 3 ]-TOG in R15LO cells with arachidonic acid treatment in the presence or absence of LO inhibitor CDC. (A) no treatment (

    Journal:

    Article Title: A 4-Oxo-2(E)-Nonenal-Derived Glutathione-Adduct from 15-Lipoxygenase-1-Mediated Oxidation of Cytosolic and Esterified Arachidonic Acid §

    doi: 10.1016/j.freeradbiomed.2009.06.035

    Figure Lengend Snippet: LC-electrospray ionization/MRM/MS analysis of TOG and its corresponding internal standard [ 2 H 3 ]-TOG in R15LO cells with arachidonic acid treatment in the presence or absence of LO inhibitor CDC. (A) no treatment (

    Article Snippet: Liquid chromatography (LC)-MRM/mass spectrometry (MS) for quantitative TOG analysis was conducted using gradient systems 1 and 2 on a Hitachi L-2130 Intelligent Pump (Hitachi, San Jose, CA).

    Techniques: Mass Spectrometry