Structured Review

Hewlett-Packard liquid chromatography mass spectrometry lc ms
Liquid Chromatography Mass Spectrometry Lc Ms, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid chromatography mass spectrometry lc ms/product/Hewlett-Packard
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
liquid chromatography mass spectrometry lc ms - by Bioz Stars, 2020-05
91/100 stars

Images

Related Articles

High Performance Liquid Chromatography:

Article Title: Heme-Dependent Rubber Oxygenase RoxA of Xanthomonas sp. Cleaves the Carbon Backbone of Poly(cis-1,4-Isoprene) by a Dioxygenase Mechanism
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) was run on an HP1100 HPLC system (Hewlett Packard, Waldbronn, Germany) comprising an HP1100 autosampler, an HP1100 gradient pump, an HP1100 column thermoregulator, and an HP1100 diode array detector module coupled to a Micromass VG Platform II quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) equipped with an electrospray ionization (ESI) interface. .. ESI+ -MS parameters were as follows: source temperature, 120°C; capillary, 3.5 V; HV lens, 0.5 kV; cone, 30 V; full scan range, m / z 120 to 450.

Generated:

Article Title: Taxol biosynthesis: Molecular cloning of a benzoyl- CoA:taxane 2?-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli
Article Snippet: .. The product generated by large-scale preparation of the putative pKW18/TAX2 benzoyltransferase (≈50 μg) was analyzed by combined liquid chromatography–mass spectrometry (LC-MS) using a Hewlett-Packard Series 1100 MSD in the APCI mode. .. The sample, dissolved in acetonitrile (200 μl), was loaded (5 μl) onto a Phenomenex (Torrance, CA) Curosil-G column (5 μm, 250 × 4.6 mm) that was eluted at 1 ml/min with 30:70 CH3 CN/H2 O for 5 min, increased linearly to 80:20 CH3 CN/H2 O over 55 min, and then held for 5 min before return to initial conditions (R. E. B. Ketchum and R.C., unpublished work).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Taxol biosynthesis: Molecular cloning of a benzoyl- CoA:taxane 2?-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli
Article Snippet: .. The product generated by large-scale preparation of the putative pKW18/TAX2 benzoyltransferase (≈50 μg) was analyzed by combined liquid chromatography–mass spectrometry (LC-MS) using a Hewlett-Packard Series 1100 MSD in the APCI mode. .. The sample, dissolved in acetonitrile (200 μl), was loaded (5 μl) onto a Phenomenex (Torrance, CA) Curosil-G column (5 μm, 250 × 4.6 mm) that was eluted at 1 ml/min with 30:70 CH3 CN/H2 O for 5 min, increased linearly to 80:20 CH3 CN/H2 O over 55 min, and then held for 5 min before return to initial conditions (R. E. B. Ketchum and R.C., unpublished work).

Article Title: Heme-Dependent Rubber Oxygenase RoxA of Xanthomonas sp. Cleaves the Carbon Backbone of Poly(cis-1,4-Isoprene) by a Dioxygenase Mechanism
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) was run on an HP1100 HPLC system (Hewlett Packard, Waldbronn, Germany) comprising an HP1100 autosampler, an HP1100 gradient pump, an HP1100 column thermoregulator, and an HP1100 diode array detector module coupled to a Micromass VG Platform II quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) equipped with an electrospray ionization (ESI) interface. .. ESI+ -MS parameters were as follows: source temperature, 120°C; capillary, 3.5 V; HV lens, 0.5 kV; cone, 30 V; full scan range, m / z 120 to 450.

Mass Spectrometry:

Article Title: Heme-Dependent Rubber Oxygenase RoxA of Xanthomonas sp. Cleaves the Carbon Backbone of Poly(cis-1,4-Isoprene) by a Dioxygenase Mechanism
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) was run on an HP1100 HPLC system (Hewlett Packard, Waldbronn, Germany) comprising an HP1100 autosampler, an HP1100 gradient pump, an HP1100 column thermoregulator, and an HP1100 diode array detector module coupled to a Micromass VG Platform II quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) equipped with an electrospray ionization (ESI) interface. .. ESI+ -MS parameters were as follows: source temperature, 120°C; capillary, 3.5 V; HV lens, 0.5 kV; cone, 30 V; full scan range, m / z 120 to 450.

Chromatography:

Article Title: Taxol biosynthesis: Molecular cloning of a benzoyl- CoA:taxane 2?-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli
Article Snippet: .. The product generated by large-scale preparation of the putative pKW18/TAX2 benzoyltransferase (≈50 μg) was analyzed by combined liquid chromatography–mass spectrometry (LC-MS) using a Hewlett-Packard Series 1100 MSD in the APCI mode. .. The sample, dissolved in acetonitrile (200 μl), was loaded (5 μl) onto a Phenomenex (Torrance, CA) Curosil-G column (5 μm, 250 × 4.6 mm) that was eluted at 1 ml/min with 30:70 CH3 CN/H2 O for 5 min, increased linearly to 80:20 CH3 CN/H2 O over 55 min, and then held for 5 min before return to initial conditions (R. E. B. Ketchum and R.C., unpublished work).

Article Title: Heme-Dependent Rubber Oxygenase RoxA of Xanthomonas sp. Cleaves the Carbon Backbone of Poly(cis-1,4-Isoprene) by a Dioxygenase Mechanism
Article Snippet: .. Liquid chromatography-mass spectrometry (LC-MS) was run on an HP1100 HPLC system (Hewlett Packard, Waldbronn, Germany) comprising an HP1100 autosampler, an HP1100 gradient pump, an HP1100 column thermoregulator, and an HP1100 diode array detector module coupled to a Micromass VG Platform II quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) equipped with an electrospray ionization (ESI) interface. .. ESI+ -MS parameters were as follows: source temperature, 120°C; capillary, 3.5 V; HV lens, 0.5 kV; cone, 30 V; full scan range, m / z 120 to 450.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Hewlett-Packard chromatography mass spectrometry lc ms
    Final HPLC purification profile and antiviral activity of Bmlipase-1. (A) Purification profile of Bmlipase-1. Bmlipase-1 was eluted with 49.5% acetonitrile-0.05% TFA by reverse-phase HPLC. The arrow indicates the Bmlipase-1 position. The inset shows the <t>LC-MS</t> spectrum with molecular <t>mass</t> of Bmlipase-1. B. mori larvae of the Daizo race were reared on mulberry leaves, whereas larvae of the Tokai × Asahi race were fed an artificial diet (Nihonnosanko). The silkworms were maintained in the silkworm rearing room under controlled environmental conditions at 27°C. The digestive juice was collected from B. mori (Daizo race) larvae in an ice-cold tube by mild electric shock, and ammonium sulfate was added to give a 40% saturation. The precipitate was suspended in 40 mM phosphate buffer (pH 7.4) and treated with 50% n -butanol (final concentration) overnight at 4°C, and the lower aqueous layer was collected after centrifugation at 10,000 × g for 30 min. Half-volume ice-cold acetone was added to this solution to precipitate proteins in the aqueous layer. The precipitated proteins were collected by centrifugation at 10,000 × g for 30 min, dried, and dissolved in 40 mM phosphate buffer (pH 7.4). The solution equilibrated with the same buffer was applied to a Superdex 200 HR 10/30 column attached to a fast-performance liquid <t>chromatography</t> system (Pharmacia). Each fraction was tested for antiviral activity. Antiviral fractions were further purified through a reverse-phase column, Sephasil C8 SC 2.1/10, attached to a SMART system (Pharmacia) with a linear gradient of acetonitrile-0.05% TFA. (B) Antiviral activity against BmNPV-ODV. Infectivity was expressed as relative light units/10 μl of hemolymph. The data shown are means ± standard deviations of results from five experiments. The infectivity of BmNPV treated with 2.2 μg of Bmlipase-1 per larva (indicated as ODV + Bmlipase-1) was compared to that of nontreated BmNPV (indicated as ODV). As a negative control, the same amount of hemolymph from nontreated B. mori ). The ODV (22.5 ng/larva) suspension was mixed with the purified protein (2.2 μg/larva) and dissolved in 40 mM phosphate buffer (pH 8.0). This mixture was incubated at 30°C for 1 h. Fifth-instar larvae just after ecdysis were orally inoculated with 5 μl of ODV mixture. Thirty larvae were used for each treatment. The hemolymph was collected at various hpi and assayed for luciferase activity. Ten microliters of hemolymph was added to 50 μl of luciferase assay buffer (Promega), and luciferase activity was measured using a Luminocounter 700 (Microtech-Nition).
    Chromatography Mass Spectrometry Lc Ms, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatography mass spectrometry lc ms/product/Hewlett-Packard
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    chromatography mass spectrometry lc ms - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    85
    Hewlett-Packard hp 1090 binary pump
    Identification of AMK and AFMK produced from melatonin by MyelPO Melatonin (1 mM) was incubated with 0.84 μg of MyelPO and 50 μM H 2 O 2 . LC separations for the LC/MS experiments were performed using an <t>HP</t> 1090 binary pump equipped with an Xterra RP 18 column. The samples were passed through a 20 μl injection loop at 1 ml/min and the flow was split to 250 μl/min into the mass spectrometer. The eluents were: A, 0.01% TFA in deionized water; B, 0.01% TFA in acetonitrile; the gradient sequence was from 0% to 80% A in 32 min. Electrospray ionization mass spectra were recorded on a Q-TOF2 instrument. ( A ) Reconstructed ionic current for m / z 178 theoretical, the most abundant fragment ion in the mass spectrum of AMK (retention time 10.6 min), and AFMK (retention time 15.1 min). ( B ) Total ionic current ( m / z 20 to 900 theoretical) showing mainly melatonin at a retention time of 17.1 min.
    Hp 1090 Binary Pump, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hp 1090 binary pump/product/Hewlett-Packard
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hp 1090 binary pump - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    88
    Hewlett-Packard quattro ii triple quadrupole mass spectrometer
    Identification of AMK and AFMK produced from melatonin by MyelPO Melatonin (1 mM) was incubated with 0.84 μg of MyelPO and 50 μM H 2 O 2 . LC separations for the LC/MS experiments were performed using an <t>HP</t> 1090 binary pump equipped with an Xterra RP 18 column. The samples were passed through a 20 μl injection loop at 1 ml/min and the flow was split to 250 μl/min into the mass spectrometer. The eluents were: A, 0.01% TFA in deionized water; B, 0.01% TFA in acetonitrile; the gradient sequence was from 0% to 80% A in 32 min. Electrospray ionization mass spectra were recorded on a Q-TOF2 instrument. ( A ) Reconstructed ionic current for m / z 178 theoretical, the most abundant fragment ion in the mass spectrum of AMK (retention time 10.6 min), and AFMK (retention time 15.1 min). ( B ) Total ionic current ( m / z 20 to 900 theoretical) showing mainly melatonin at a retention time of 17.1 min.
    Quattro Ii Triple Quadrupole Mass Spectrometer, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quattro ii triple quadrupole mass spectrometer/product/Hewlett-Packard
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quattro ii triple quadrupole mass spectrometer - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Hewlett-Packard 1100 lc ms chemstation
    Identification of AMK and AFMK produced from melatonin by MyelPO Melatonin (1 mM) was incubated with 0.84 μg of MyelPO and 50 μM H 2 O 2 . LC separations for the LC/MS experiments were performed using an <t>HP</t> 1090 binary pump equipped with an Xterra RP 18 column. The samples were passed through a 20 μl injection loop at 1 ml/min and the flow was split to 250 μl/min into the mass spectrometer. The eluents were: A, 0.01% TFA in deionized water; B, 0.01% TFA in acetonitrile; the gradient sequence was from 0% to 80% A in 32 min. Electrospray ionization mass spectra were recorded on a Q-TOF2 instrument. ( A ) Reconstructed ionic current for m / z 178 theoretical, the most abundant fragment ion in the mass spectrum of AMK (retention time 10.6 min), and AFMK (retention time 15.1 min). ( B ) Total ionic current ( m / z 20 to 900 theoretical) showing mainly melatonin at a retention time of 17.1 min.
    1100 Lc Ms Chemstation, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1100 lc ms chemstation/product/Hewlett-Packard
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    1100 lc ms chemstation - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    Final HPLC purification profile and antiviral activity of Bmlipase-1. (A) Purification profile of Bmlipase-1. Bmlipase-1 was eluted with 49.5% acetonitrile-0.05% TFA by reverse-phase HPLC. The arrow indicates the Bmlipase-1 position. The inset shows the LC-MS spectrum with molecular mass of Bmlipase-1. B. mori larvae of the Daizo race were reared on mulberry leaves, whereas larvae of the Tokai × Asahi race were fed an artificial diet (Nihonnosanko). The silkworms were maintained in the silkworm rearing room under controlled environmental conditions at 27°C. The digestive juice was collected from B. mori (Daizo race) larvae in an ice-cold tube by mild electric shock, and ammonium sulfate was added to give a 40% saturation. The precipitate was suspended in 40 mM phosphate buffer (pH 7.4) and treated with 50% n -butanol (final concentration) overnight at 4°C, and the lower aqueous layer was collected after centrifugation at 10,000 × g for 30 min. Half-volume ice-cold acetone was added to this solution to precipitate proteins in the aqueous layer. The precipitated proteins were collected by centrifugation at 10,000 × g for 30 min, dried, and dissolved in 40 mM phosphate buffer (pH 7.4). The solution equilibrated with the same buffer was applied to a Superdex 200 HR 10/30 column attached to a fast-performance liquid chromatography system (Pharmacia). Each fraction was tested for antiviral activity. Antiviral fractions were further purified through a reverse-phase column, Sephasil C8 SC 2.1/10, attached to a SMART system (Pharmacia) with a linear gradient of acetonitrile-0.05% TFA. (B) Antiviral activity against BmNPV-ODV. Infectivity was expressed as relative light units/10 μl of hemolymph. The data shown are means ± standard deviations of results from five experiments. The infectivity of BmNPV treated with 2.2 μg of Bmlipase-1 per larva (indicated as ODV + Bmlipase-1) was compared to that of nontreated BmNPV (indicated as ODV). As a negative control, the same amount of hemolymph from nontreated B. mori ). The ODV (22.5 ng/larva) suspension was mixed with the purified protein (2.2 μg/larva) and dissolved in 40 mM phosphate buffer (pH 8.0). This mixture was incubated at 30°C for 1 h. Fifth-instar larvae just after ecdysis were orally inoculated with 5 μl of ODV mixture. Thirty larvae were used for each treatment. The hemolymph was collected at various hpi and assayed for luciferase activity. Ten microliters of hemolymph was added to 50 μl of luciferase assay buffer (Promega), and luciferase activity was measured using a Luminocounter 700 (Microtech-Nition).

    Journal: Journal of Virology

    Article Title: A Lipase Isolated from the Silkworm Bombyx mori Shows Antiviral Activity against Nucleopolyhedrovirus

    doi: 10.1128/JVI.77.19.10725-10729.2003

    Figure Lengend Snippet: Final HPLC purification profile and antiviral activity of Bmlipase-1. (A) Purification profile of Bmlipase-1. Bmlipase-1 was eluted with 49.5% acetonitrile-0.05% TFA by reverse-phase HPLC. The arrow indicates the Bmlipase-1 position. The inset shows the LC-MS spectrum with molecular mass of Bmlipase-1. B. mori larvae of the Daizo race were reared on mulberry leaves, whereas larvae of the Tokai × Asahi race were fed an artificial diet (Nihonnosanko). The silkworms were maintained in the silkworm rearing room under controlled environmental conditions at 27°C. The digestive juice was collected from B. mori (Daizo race) larvae in an ice-cold tube by mild electric shock, and ammonium sulfate was added to give a 40% saturation. The precipitate was suspended in 40 mM phosphate buffer (pH 7.4) and treated with 50% n -butanol (final concentration) overnight at 4°C, and the lower aqueous layer was collected after centrifugation at 10,000 × g for 30 min. Half-volume ice-cold acetone was added to this solution to precipitate proteins in the aqueous layer. The precipitated proteins were collected by centrifugation at 10,000 × g for 30 min, dried, and dissolved in 40 mM phosphate buffer (pH 7.4). The solution equilibrated with the same buffer was applied to a Superdex 200 HR 10/30 column attached to a fast-performance liquid chromatography system (Pharmacia). Each fraction was tested for antiviral activity. Antiviral fractions were further purified through a reverse-phase column, Sephasil C8 SC 2.1/10, attached to a SMART system (Pharmacia) with a linear gradient of acetonitrile-0.05% TFA. (B) Antiviral activity against BmNPV-ODV. Infectivity was expressed as relative light units/10 μl of hemolymph. The data shown are means ± standard deviations of results from five experiments. The infectivity of BmNPV treated with 2.2 μg of Bmlipase-1 per larva (indicated as ODV + Bmlipase-1) was compared to that of nontreated BmNPV (indicated as ODV). As a negative control, the same amount of hemolymph from nontreated B. mori ). The ODV (22.5 ng/larva) suspension was mixed with the purified protein (2.2 μg/larva) and dissolved in 40 mM phosphate buffer (pH 8.0). This mixture was incubated at 30°C for 1 h. Fifth-instar larvae just after ecdysis were orally inoculated with 5 μl of ODV mixture. Thirty larvae were used for each treatment. The hemolymph was collected at various hpi and assayed for luciferase activity. Ten microliters of hemolymph was added to 50 μl of luciferase assay buffer (Promega), and luciferase activity was measured using a Luminocounter 700 (Microtech-Nition).

    Article Snippet: The homogeneity of the antiviral peak fraction was examined both by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using a Voyager RP biospectrometry system (PerSeptive Biosystems) and by liquid chromatography-mass spectrometry (LC-MS) using a Series 1100MSD (Hewlett-Packard).

    Techniques: High Performance Liquid Chromatography, Purification, Activity Assay, Liquid Chromatography with Mass Spectroscopy, Concentration Assay, Centrifugation, Liquid Chromatography, Infection, Negative Control, Incubation, Luciferase

    Identification of AMK and AFMK produced from melatonin by MyelPO Melatonin (1 mM) was incubated with 0.84 μg of MyelPO and 50 μM H 2 O 2 . LC separations for the LC/MS experiments were performed using an HP 1090 binary pump equipped with an Xterra RP 18 column. The samples were passed through a 20 μl injection loop at 1 ml/min and the flow was split to 250 μl/min into the mass spectrometer. The eluents were: A, 0.01% TFA in deionized water; B, 0.01% TFA in acetonitrile; the gradient sequence was from 0% to 80% A in 32 min. Electrospray ionization mass spectra were recorded on a Q-TOF2 instrument. ( A ) Reconstructed ionic current for m / z 178 theoretical, the most abundant fragment ion in the mass spectrum of AMK (retention time 10.6 min), and AFMK (retention time 15.1 min). ( B ) Total ionic current ( m / z 20 to 900 theoretical) showing mainly melatonin at a retention time of 17.1 min.

    Journal: Biochemical Journal

    Article Title: Molecular evidence that melatonin is enzymatically oxidized in a different manner than tryptophan: investigations with both indoleamine 2,3-dioxygenase and myeloperoxidase

    doi: 10.1042/BJ20042075

    Figure Lengend Snippet: Identification of AMK and AFMK produced from melatonin by MyelPO Melatonin (1 mM) was incubated with 0.84 μg of MyelPO and 50 μM H 2 O 2 . LC separations for the LC/MS experiments were performed using an HP 1090 binary pump equipped with an Xterra RP 18 column. The samples were passed through a 20 μl injection loop at 1 ml/min and the flow was split to 250 μl/min into the mass spectrometer. The eluents were: A, 0.01% TFA in deionized water; B, 0.01% TFA in acetonitrile; the gradient sequence was from 0% to 80% A in 32 min. Electrospray ionization mass spectra were recorded on a Q-TOF2 instrument. ( A ) Reconstructed ionic current for m / z 178 theoretical, the most abundant fragment ion in the mass spectrum of AMK (retention time 10.6 min), and AFMK (retention time 15.1 min). ( B ) Total ionic current ( m / z 20 to 900 theoretical) showing mainly melatonin at a retention time of 17.1 min.

    Article Snippet: LC separations for the LC/MS experiments were performed using an HP 1090 binary pump equipped with a UV detector (Hewlett Packard, Palo Alto, CA, U.S.A.).

    Techniques: Produced, Incubation, Liquid Chromatography with Mass Spectroscopy, Injection, Flow Cytometry, Mass Spectrometry, Sequencing