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Agilent technologies liquid chromatography mass spectrometry lc ms
Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by <t>liquid</t> <t>chromatography-mass</t> <t>spectrometry</t> and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P
Liquid Chromatography Mass Spectrometry Lc Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats"

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats

Journal: Pharmacognosy Magazine

doi: 10.4103/pm.pm_340_17

Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P
Figure Legend Snippet: Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

Techniques Used: Liquid Chromatography, Mass Spectrometry, Concentration Assay, Standard Deviation

2) Product Images from "Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry"

Article Title: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

Journal: BMC Biotechnology

doi: 10.1186/1472-6750-12-51

Reconstructed ion chromatograms of the hydrolyzed collagen samples. Samples were separated by reversed-phase liquid chromatography and detected by ESI mass spectrometry. (A) Bovine collagen III, as received, (B) Bovine collagen III extracted from SDS-PAGE, and (C) Recombinant human collagen III. Rows: Top, IS = internal standard; Middle, HYP = hydroxyproline; Bottom, PRO = proline.
Figure Legend Snippet: Reconstructed ion chromatograms of the hydrolyzed collagen samples. Samples were separated by reversed-phase liquid chromatography and detected by ESI mass spectrometry. (A) Bovine collagen III, as received, (B) Bovine collagen III extracted from SDS-PAGE, and (C) Recombinant human collagen III. Rows: Top, IS = internal standard; Middle, HYP = hydroxyproline; Bottom, PRO = proline.

Techniques Used: Liquid Chromatography, Mass Spectrometry, SDS Page, Recombinant

3) Product Images from "Rapid, low-cost fluorescent assay of [... formula ...]-lactamase-derived antibiotic resistance and related antibiotic susceptibility"

Article Title: Rapid, low-cost fluorescent assay of [... formula ...]-lactamase-derived antibiotic resistance and related antibiotic susceptibility

Journal: Journal of Biomedical Optics

doi: 10.1117/1.JBO.19.10.105007

Liquid chromatography mass spectrometry (LC-MS) analysis of the \u03b2-LEAF Bodipy-FL probe.
Figure Legend Snippet: Liquid chromatography mass spectrometry (LC-MS) analysis of the \u03b2-LEAF Bodipy-FL probe.

Techniques Used: Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

4) Product Images from "Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats"

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats

Journal: Pharmacognosy Magazine

doi: 10.4103/pm.pm_340_17

Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P
Figure Legend Snippet: Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

Techniques Used: Liquid Chromatography, Mass Spectrometry, Concentration Assay, Standard Deviation

5) Product Images from "Metabolomic Profiling Reveals a Role for Androgen in Activating Amino Acid Metabolism and Methylation in Prostate Cancer Cells"

Article Title: Metabolomic Profiling Reveals a Role for Androgen in Activating Amino Acid Metabolism and Methylation in Prostate Cancer Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0021417

Metabolome of prostate cancer cell lines. A ) Heat map representation of hierarchical clustering of 1,553 metabolites across 5 prostate cell lines. Sample classes are indicated by the colored bars [benign = green, androgen non-responsive PCa (ARI): yellow and androgen responsive PCa (ARD): red bar]. Columns represent individual cell lines and rows refer to distinct metabolites. Shades of yellow represent elevation of a metabolite and shades of blue represent decrease of a metabolite relative to the median metabolite levels (see color scale). B ) Venn diagram representing the distribution of 1,553 metabolites measured across benign (RWPE), ARI (DU145 and PC3) and ARD (LNCaP and VCaP) cell lines using liquid chromatography coupled mass spectrometry. C ) same as in ( A ), but for 72 named compounds D ) Dendrogram representing hierarchical clustering of the prostate-related cell lines described in B, using compounds with significant differential expression.
Figure Legend Snippet: Metabolome of prostate cancer cell lines. A ) Heat map representation of hierarchical clustering of 1,553 metabolites across 5 prostate cell lines. Sample classes are indicated by the colored bars [benign = green, androgen non-responsive PCa (ARI): yellow and androgen responsive PCa (ARD): red bar]. Columns represent individual cell lines and rows refer to distinct metabolites. Shades of yellow represent elevation of a metabolite and shades of blue represent decrease of a metabolite relative to the median metabolite levels (see color scale). B ) Venn diagram representing the distribution of 1,553 metabolites measured across benign (RWPE), ARI (DU145 and PC3) and ARD (LNCaP and VCaP) cell lines using liquid chromatography coupled mass spectrometry. C ) same as in ( A ), but for 72 named compounds D ) Dendrogram representing hierarchical clustering of the prostate-related cell lines described in B, using compounds with significant differential expression.

Techniques Used: Liquid Chromatography, Mass Spectrometry, Expressing

Related Articles

Chromatography:

Article Title: The Phage Lytic Proteins from the Staphylococcus aureus Bacteriophage vB_SauS-phiIPLA88 Display Multiple Active Catalytic Domains and Do Not Trigger Staphylococcal Resistance
Article Snippet: .. Peaks were then analysed by Liquid Chromatography-Mass Spectrometry (LC-MS) (Agilent Technologies, Waldbronn, Germany). .. Determination of Bacterial Resistance to Lytic Proteins Resistant development was tested using repeated exposures in both the plate lysis and the minimal inhibitory concentration (MIC) assays.

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

Article Title: SnPKS19 Encodes the Polyketide Synthase for Alternariol Mycotoxin Biosynthesis in the Wheat Pathogen Parastagonospora nodorum
Article Snippet: .. For the detection of AOH and AME in the P. nodorum SN15 extracts from various growth conditions, liquid chromatography-mass spectrometry (LC-MS) was performed on an Agilent 1200 LC system (Agilent, Santa Clara, CA, USA) coupled to an Agilent 6520 quadrupole time of flight (QToF) system with a Jetstream electrospray ionization (ESI) source to achieve higher sensitivity (see the methods in the supplemental material). ..

Article Title: Metabolomic Profiling Reveals a Role for Androgen in Activating Amino Acid Metabolism and Methylation in Prostate Cancer Cells
Article Snippet: .. Liquid Chromatography/Mass Spectrometry (LC/MS) The LC/MS portion of the unbiased profiling platform is based on a 1200 SL Rapid resolution LC and a 6520 Quadrapole Time Of Flight (Q-TOF) mass spectrometer (Agilent Technologies, Santa Clara, CA). ..

Article Title: Lysophosphatidic Acid Signaling May Initiate Fetal Hydrocephalus
Article Snippet: .. The extracts were subjected to liquid chromatography-mass spectrometry (LC-MS) for LPA measurement at the TSRI Mass Spectrometry Core using an Agilent 6410 triple quad mass spectrometer coupled to an Agilent 1200lc stack. ..

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. Equipments All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

Liquid Chromatography:

Article Title: Rapid, low-cost fluorescent assay of [... formula ...]-lactamase-derived antibiotic resistance and related antibiotic susceptibility
Article Snippet: .. Liquid chromatography mass spectrometry (LC-MS) was obtained using Agilent 6430 Triple Quad LC/MS system (Santa Clara, California). .. High-performance liquid chromatography (HPLC) was performed with reverse phase Alltima C18 column using a Shimadzu SCL-10AVP controller with a SPD-M10AVP photodiode array detector (Kyoto, Japan).

Article Title: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry
Article Snippet: .. Liquid Chromatography – Mass Spectrometry (LC-MS) method The LC-MS consisted of an Agilent 1100 instrument and a Waters LCT Classic mass spectrometer in an open access user facility. .. The liquid chromatography separations used a solvent system of 2% acetonitrile and 0.2% acetic acid in water (solvent A) and 0.2% acetic acid in acetonitrile (solvent B), with a 45-minute solvent program that reached 25% B at 25 min followed by a rapid ramp to 95% B to remove unwanted compounds from the column.

Spectrophotometry:

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. Equipments All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

Mass Spectrometry:

Article Title: Rapid, low-cost fluorescent assay of [... formula ...]-lactamase-derived antibiotic resistance and related antibiotic susceptibility
Article Snippet: .. Liquid chromatography mass spectrometry (LC-MS) was obtained using Agilent 6430 Triple Quad LC/MS system (Santa Clara, California). .. High-performance liquid chromatography (HPLC) was performed with reverse phase Alltima C18 column using a Shimadzu SCL-10AVP controller with a SPD-M10AVP photodiode array detector (Kyoto, Japan).

Article Title: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry
Article Snippet: .. Liquid Chromatography – Mass Spectrometry (LC-MS) method The LC-MS consisted of an Agilent 1100 instrument and a Waters LCT Classic mass spectrometer in an open access user facility. .. The liquid chromatography separations used a solvent system of 2% acetonitrile and 0.2% acetic acid in water (solvent A) and 0.2% acetic acid in acetonitrile (solvent B), with a 45-minute solvent program that reached 25% B at 25 min followed by a rapid ramp to 95% B to remove unwanted compounds from the column.

Article Title: Metabolomic Profiling Reveals a Role for Androgen in Activating Amino Acid Metabolism and Methylation in Prostate Cancer Cells
Article Snippet: .. Liquid Chromatography/Mass Spectrometry (LC/MS) The LC/MS portion of the unbiased profiling platform is based on a 1200 SL Rapid resolution LC and a 6520 Quadrapole Time Of Flight (Q-TOF) mass spectrometer (Agilent Technologies, Santa Clara, CA). ..

Article Title: Lysophosphatidic Acid Signaling May Initiate Fetal Hydrocephalus
Article Snippet: .. The extracts were subjected to liquid chromatography-mass spectrometry (LC-MS) for LPA measurement at the TSRI Mass Spectrometry Core using an Agilent 6410 triple quad mass spectrometer coupled to an Agilent 1200lc stack. ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: The Phage Lytic Proteins from the Staphylococcus aureus Bacteriophage vB_SauS-phiIPLA88 Display Multiple Active Catalytic Domains and Do Not Trigger Staphylococcal Resistance
Article Snippet: .. Peaks were then analysed by Liquid Chromatography-Mass Spectrometry (LC-MS) (Agilent Technologies, Waldbronn, Germany). .. Determination of Bacterial Resistance to Lytic Proteins Resistant development was tested using repeated exposures in both the plate lysis and the minimal inhibitory concentration (MIC) assays.

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

Article Title: Rapid, low-cost fluorescent assay of [... formula ...]-lactamase-derived antibiotic resistance and related antibiotic susceptibility
Article Snippet: .. Liquid chromatography mass spectrometry (LC-MS) was obtained using Agilent 6430 Triple Quad LC/MS system (Santa Clara, California). .. High-performance liquid chromatography (HPLC) was performed with reverse phase Alltima C18 column using a Shimadzu SCL-10AVP controller with a SPD-M10AVP photodiode array detector (Kyoto, Japan).

Article Title: SnPKS19 Encodes the Polyketide Synthase for Alternariol Mycotoxin Biosynthesis in the Wheat Pathogen Parastagonospora nodorum
Article Snippet: .. For the detection of AOH and AME in the P. nodorum SN15 extracts from various growth conditions, liquid chromatography-mass spectrometry (LC-MS) was performed on an Agilent 1200 LC system (Agilent, Santa Clara, CA, USA) coupled to an Agilent 6520 quadrupole time of flight (QToF) system with a Jetstream electrospray ionization (ESI) source to achieve higher sensitivity (see the methods in the supplemental material). ..

Article Title: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry
Article Snippet: .. Liquid Chromatography – Mass Spectrometry (LC-MS) method The LC-MS consisted of an Agilent 1100 instrument and a Waters LCT Classic mass spectrometer in an open access user facility. .. The liquid chromatography separations used a solvent system of 2% acetonitrile and 0.2% acetic acid in water (solvent A) and 0.2% acetic acid in acetonitrile (solvent B), with a 45-minute solvent program that reached 25% B at 25 min followed by a rapid ramp to 95% B to remove unwanted compounds from the column.

Article Title: Metabolomic Profiling Reveals a Role for Androgen in Activating Amino Acid Metabolism and Methylation in Prostate Cancer Cells
Article Snippet: .. Liquid Chromatography/Mass Spectrometry (LC/MS) The LC/MS portion of the unbiased profiling platform is based on a 1200 SL Rapid resolution LC and a 6520 Quadrapole Time Of Flight (Q-TOF) mass spectrometer (Agilent Technologies, Santa Clara, CA). ..

Article Title: Lysophosphatidic Acid Signaling May Initiate Fetal Hydrocephalus
Article Snippet: .. The extracts were subjected to liquid chromatography-mass spectrometry (LC-MS) for LPA measurement at the TSRI Mass Spectrometry Core using an Agilent 6410 triple quad mass spectrometer coupled to an Agilent 1200lc stack. ..

Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats
Article Snippet: .. Equipments All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly. ..

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    Agilent technologies lc uv ms ms based reverse phase chiral analysis
    <t>LC-UV-MS-MS</t> <t>based</t> <t>chiral</t> <t>analysis</t> shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.
    Lc Uv Ms Ms Based Reverse Phase Chiral Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies triple quadrupole mass spectrometer detector
    Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a <t>triple</t> <t>quadrupole</t> <t>mass</t> <t>spectrometer</t> as reported in Methods section.
    Triple Quadrupole Mass Spectrometer Detector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies lc ims cid ms data
    Addition of the <t>IMS</t> dimension enables better quantitation of MS1 data. Calibration curves for 10 of the <t>BSA</t> target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.
    Lc Ims Cid Ms Data, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies mass spectrometry nano lc chip q tof
    Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by <t>nano-LC</t> Chip <t>Q-ToF</t> MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p
    Mass Spectrometry Nano Lc Chip Q Tof, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    LC-UV-MS-MS based chiral analysis shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.

    Journal: Current Protocols in Immunology

    Article Title: Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated By Phagocytes

    doi: 10.1002/0471142735.im1426s95

    Figure Lengend Snippet: LC-UV-MS-MS based chiral analysis shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.

    Article Snippet: LC-UV-MS-MS based reverse phase chiral analysis is carried out using ABI 3200 Qtrap mass spectrometer coupled with an Agilent 1100 series HPLC with a ChiralPak AD-RH reverse phase chiral column (Chiral Technology, West Chester, PA) (15).

    Techniques: Mass Spectrometry, Incubation, Generated, Diagnostic Assay

    LC-UV-MS-MS based chiral analysis of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture. Chiralities are determined by marching retention time with optical pure compounds. Total Ion Chromatogram (TIC) of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture is shown in brown color. Chromatogram of precursor/product ion pair m/z 343 > 101 for racemic 4-HDHA is shown in blue, m/z 343 > 141 for 7-HDHA in orange, and m/z 343 > 205 for 14-HDHA in green.

    Journal: Current Protocols in Immunology

    Article Title: Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated By Phagocytes

    doi: 10.1002/0471142735.im1426s95

    Figure Lengend Snippet: LC-UV-MS-MS based chiral analysis of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture. Chiralities are determined by marching retention time with optical pure compounds. Total Ion Chromatogram (TIC) of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture is shown in brown color. Chromatogram of precursor/product ion pair m/z 343 > 101 for racemic 4-HDHA is shown in blue, m/z 343 > 141 for 7-HDHA in orange, and m/z 343 > 205 for 14-HDHA in green.

    Article Snippet: LC-UV-MS-MS based reverse phase chiral analysis is carried out using ABI 3200 Qtrap mass spectrometer coupled with an Agilent 1100 series HPLC with a ChiralPak AD-RH reverse phase chiral column (Chiral Technology, West Chester, PA) (15).

    Techniques: Mass Spectrometry

    Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a triple quadrupole mass spectrometer as reported in Methods section.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

    doi: 10.1155/2015/318125

    Figure Lengend Snippet: Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a triple quadrupole mass spectrometer as reported in Methods section.

    Article Snippet: For the structural elucidation and the detection of other compounds such as hibiscus acid, a typical compound present in HSE, the HPLC system was coupled online to a triple quadrupole mass spectrometer detector (QqQ, 6420 Triple Quad/LC MS, Agilent Technologies) equipped with a TurboIonSpray source operating in negative-ion mode.

    Techniques: Ion Chromatography, Liquid Chromatography, Mass Spectrometry

    Addition of the IMS dimension enables better quantitation of MS1 data. Calibration curves for 10 of the BSA target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Addition of the IMS dimension enables better quantitation of MS1 data. Calibration curves for 10 of the BSA target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Quantitation Assay

    Interferences increase at lower concentrations in the all-ions fragmentation spectra when IMS filtering is not utilized. LC-CID-MS spectra for the transition ions of SLHTLFGDELC[+57]K 2+ are shown at 100 pM A ) without and B ) with IMS filtering. The plots on the right of each XIC show two ions co-eluting in the LC dimension, but being separated by IMS. The transition of interest occurs at 29 ms, while the interfering ion is at 33 ms, causing noise around the transitions if IMS filtering is not utilized.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Interferences increase at lower concentrations in the all-ions fragmentation spectra when IMS filtering is not utilized. LC-CID-MS spectra for the transition ions of SLHTLFGDELC[+57]K 2+ are shown at 100 pM A ) without and B ) with IMS filtering. The plots on the right of each XIC show two ions co-eluting in the LC dimension, but being separated by IMS. The transition of interest occurs at 29 ms, while the interfering ion is at 33 ms, causing noise around the transitions if IMS filtering is not utilized.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Mass Spectrometry

    Precursor and fragment ions have the same IMS drift times in the IMS-MS and IMS-CID-MS spectra. Plots for 5 nM BSA spiked into yeast illustrating the A ) IMS-MS and B ) IMS-CID-MS spectra taken consecutively at 17.74 and 17.75 min. The fragment ions in B ) can be observed at the same drift times as their precursors in A ) due to IMS separation followed by CID. Two different regions are highlighted illustrated the precursors and corresponding fragments.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Precursor and fragment ions have the same IMS drift times in the IMS-MS and IMS-CID-MS spectra. Plots for 5 nM BSA spiked into yeast illustrating the A ) IMS-MS and B ) IMS-CID-MS spectra taken consecutively at 17.74 and 17.75 min. The fragment ions in B ) can be observed at the same drift times as their precursors in A ) due to IMS separation followed by CID. Two different regions are highlighted illustrated the precursors and corresponding fragments.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Mass Spectrometry

    Addition of the IMS dimension enables better quantitation in fragmentation data. Calibration curves for 10 BSA target peptides spiked into yeast and extracted in the A ) LC-CID-MS and B ) LC-IMS-CID-MS all-ions fragmentation spectra. Like the MS1 spectra, IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise. A specific example of interference is illustrated in the bottom XICs for the baseline area around the transitions of YiC[+57]DNQDTISSK + in the 10 nM BSA/yeast sample.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Addition of the IMS dimension enables better quantitation in fragmentation data. Calibration curves for 10 BSA target peptides spiked into yeast and extracted in the A ) LC-CID-MS and B ) LC-IMS-CID-MS all-ions fragmentation spectra. Like the MS1 spectra, IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise. A specific example of interference is illustrated in the bottom XICs for the baseline area around the transitions of YiC[+57]DNQDTISSK + in the 10 nM BSA/yeast sample.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Quantitation Assay, Mass Spectrometry

    Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by nano-LC Chip Q-ToF MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p

    Journal: Catalysts (Basel, Switzerland)

    Article Title: Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans

    doi: 10.3390/catal8070278

    Figure Lengend Snippet: Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by nano-LC Chip Q-ToF MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p

    Article Snippet: N-glycan Analysis by Mass Spectrometry Nano-LC-Chip Q ToF Samples were transferred to polypropylene vials and 6 μL were injected into an Agilent 6520 nano-LC-Chip quadrupole time-of-flight mass spectrometer (Q-ToF MS, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Chromatin Immunoprecipitation, Mass Spectrometry, Standard Deviation