liquid chromatography mass spectrometry lc ms system  (Agilent technologies)

 
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    Agilent technologies liquid chromatography mass spectrometry lc ms system
    Liquid Chromatography Mass Spectrometry Lc Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography mass spectrometry lc ms system/product/Agilent technologies
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography mass spectrometry lc ms system - by Bioz Stars, 2020-05
    92/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Generation and Characterization of a Novel Cyp2a(4/5)bgs-Null Mouse Model
    Article Snippet: .. Testosterone metabolites were determined using a liquid chromatography-mass spectrometry (LC-MS) system consisting of an Agilent 1200 Series HPLC and an ABI 4000 Q-Trap mass spectrometer (Applied Biosystem, Foster City, CA), fitted with a 1.8-µm XDB-C18 column (4.6 × 50 mm; Agilent Technologies, Santa Clara, CA). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Bioassay for monitoring the anti-aging effect of cord blood treatment
    Article Snippet: .. The blood samples were analyzed using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent). .. We converted LC/MS files into a standard mzXML format (ProteoWizard) and then processed them using MAIT (metabolite automatic identification toolkit) for feature detection, statistical analysis, and metabolite identification.

    Article Title: An Evolutionarily Conserved Mechanism for Intrinsic and Transferable Polymyxin Resistance
    Article Snippet: .. LC-MS. As in our recently conducted experiments , the identities of both the substrate of NBD-glycerol-3-PEA and the product of NBD-glycerol (from the MCR-1-catalyzed half reaction) were determined by using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent Technologies 6460 triple-quadrupole LC-MS) ( ). .. The electrospray ionization (ESI) source was connected with the mass spectrometer, and the neutral loss ion (m/z , 141) mode was set to scan the positive ion.

    Article Title: Generation and Characterization of a Novel Cyp2a(4/5)bgs-Null Mouse Model
    Article Snippet: .. Testosterone metabolites were determined using a liquid chromatography-mass spectrometry (LC-MS) system consisting of an Agilent 1200 Series HPLC and an ABI 4000 Q-Trap mass spectrometer (Applied Biosystem, Foster City, CA), fitted with a 1.8-µm XDB-C18 column (4.6 × 50 mm; Agilent Technologies, Santa Clara, CA). ..

    Article Title: An Evolutionarily Conserved Mechanism for Intrinsic and Transferable Polymyxin Resistance
    Article Snippet: .. As in our recently conducted experiments , the identities of both the substrate of NBD-glycerol-3-PEA and the product of NBD-glycerol (from the MCR-1-catalyzed half reaction) were determined by using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent Technologies 6460 triple-quadrupole LC-MS) ( ). .. The electrospray ionization (ESI) source was connected with the mass spectrometer, and the neutral loss ion ( m/z , 141) mode was set to scan the positive ion.

    Mass Spectrometry:

    Article Title: Generation and Characterization of a Novel Cyp2a(4/5)bgs-Null Mouse Model
    Article Snippet: .. Testosterone metabolites were determined using a liquid chromatography-mass spectrometry (LC-MS) system consisting of an Agilent 1200 Series HPLC and an ABI 4000 Q-Trap mass spectrometer (Applied Biosystem, Foster City, CA), fitted with a 1.8-µm XDB-C18 column (4.6 × 50 mm; Agilent Technologies, Santa Clara, CA). ..

    Chromatography:

    Article Title: Bioassay for monitoring the anti-aging effect of cord blood treatment
    Article Snippet: .. The blood samples were analyzed using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent). .. We converted LC/MS files into a standard mzXML format (ProteoWizard) and then processed them using MAIT (metabolite automatic identification toolkit) for feature detection, statistical analysis, and metabolite identification.

    Article Title: An Evolutionarily Conserved Mechanism for Intrinsic and Transferable Polymyxin Resistance
    Article Snippet: .. LC-MS. As in our recently conducted experiments , the identities of both the substrate of NBD-glycerol-3-PEA and the product of NBD-glycerol (from the MCR-1-catalyzed half reaction) were determined by using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent Technologies 6460 triple-quadrupole LC-MS) ( ). .. The electrospray ionization (ESI) source was connected with the mass spectrometer, and the neutral loss ion (m/z , 141) mode was set to scan the positive ion.

    Article Title: Generation and Characterization of a Novel Cyp2a(4/5)bgs-Null Mouse Model
    Article Snippet: .. Testosterone metabolites were determined using a liquid chromatography-mass spectrometry (LC-MS) system consisting of an Agilent 1200 Series HPLC and an ABI 4000 Q-Trap mass spectrometer (Applied Biosystem, Foster City, CA), fitted with a 1.8-µm XDB-C18 column (4.6 × 50 mm; Agilent Technologies, Santa Clara, CA). ..

    Article Title: An Evolutionarily Conserved Mechanism for Intrinsic and Transferable Polymyxin Resistance
    Article Snippet: .. As in our recently conducted experiments , the identities of both the substrate of NBD-glycerol-3-PEA and the product of NBD-glycerol (from the MCR-1-catalyzed half reaction) were determined by using a liquid chromatography-mass spectrometry (LC-MS) system (Agilent Technologies 6460 triple-quadrupole LC-MS) ( ). .. The electrospray ionization (ESI) source was connected with the mass spectrometer, and the neutral loss ion ( m/z , 141) mode was set to scan the positive ion.

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    Agilent technologies lc uv ms ms based reverse phase chiral analysis
    <t>LC-UV-MS-MS</t> <t>based</t> <t>chiral</t> <t>analysis</t> shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.
    Lc Uv Ms Ms Based Reverse Phase Chiral Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc uv ms ms based reverse phase chiral analysis/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Agilent technologies triple quadrupole mass spectrometer detector
    Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a <t>triple</t> <t>quadrupole</t> <t>mass</t> <t>spectrometer</t> as reported in Methods section.
    Triple Quadrupole Mass Spectrometer Detector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies lc ims cid ms data
    Addition of the <t>IMS</t> dimension enables better quantitation of MS1 data. Calibration curves for 10 of the <t>BSA</t> target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.
    Lc Ims Cid Ms Data, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies mass spectrometry nano lc chip q tof
    Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by <t>nano-LC</t> Chip <t>Q-ToF</t> MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p
    Mass Spectrometry Nano Lc Chip Q Tof, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mass spectrometry nano lc chip q tof/product/Agilent technologies
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    LC-UV-MS-MS based chiral analysis shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.

    Journal: Current Protocols in Immunology

    Article Title: Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated By Phagocytes

    doi: 10.1002/0471142735.im1426s95

    Figure Lengend Snippet: LC-UV-MS-MS based chiral analysis shows incubation of porcine 12-LOX with DHA generated product with > 98% S-configuration. The incubation was reduced with NaBH 4 before analysis. The assignment was confirmed by co-eluting (retention time), online UV and diagnostic ions of ms/ms spectrum with authentic synthetic standards. The spectrum of 14-HDHA is shown on the right.

    Article Snippet: LC-UV-MS-MS based reverse phase chiral analysis is carried out using ABI 3200 Qtrap mass spectrometer coupled with an Agilent 1100 series HPLC with a ChiralPak AD-RH reverse phase chiral column (Chiral Technology, West Chester, PA) (15).

    Techniques: Mass Spectrometry, Incubation, Generated, Diagnostic Assay

    LC-UV-MS-MS based chiral analysis of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture. Chiralities are determined by marching retention time with optical pure compounds. Total Ion Chromatogram (TIC) of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture is shown in brown color. Chromatogram of precursor/product ion pair m/z 343 > 101 for racemic 4-HDHA is shown in blue, m/z 343 > 141 for 7-HDHA in orange, and m/z 343 > 205 for 14-HDHA in green.

    Journal: Current Protocols in Immunology

    Article Title: Metabolomics-Lipidomics of Eicosanoids and Docosanoids Generated By Phagocytes

    doi: 10.1002/0471142735.im1426s95

    Figure Lengend Snippet: LC-UV-MS-MS based chiral analysis of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture. Chiralities are determined by marching retention time with optical pure compounds. Total Ion Chromatogram (TIC) of racemic 4-HDHA, 7-HDHA, and 14-HDHA mixture is shown in brown color. Chromatogram of precursor/product ion pair m/z 343 > 101 for racemic 4-HDHA is shown in blue, m/z 343 > 141 for 7-HDHA in orange, and m/z 343 > 205 for 14-HDHA in green.

    Article Snippet: LC-UV-MS-MS based reverse phase chiral analysis is carried out using ABI 3200 Qtrap mass spectrometer coupled with an Agilent 1100 series HPLC with a ChiralPak AD-RH reverse phase chiral column (Chiral Technology, West Chester, PA) (15).

    Techniques: Mass Spectrometry

    Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a triple quadrupole mass spectrometer as reported in Methods section.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

    doi: 10.1155/2015/318125

    Figure Lengend Snippet: Phenolic profile of OEE at λ = 280 and 320 nm and extracted ion chromatography of hibiscus acid. The analysis of phenolic compounds was performed by liquid chromatography coupled online with a UV-Vis detection and a triple quadrupole mass spectrometer as reported in Methods section.

    Article Snippet: For the structural elucidation and the detection of other compounds such as hibiscus acid, a typical compound present in HSE, the HPLC system was coupled online to a triple quadrupole mass spectrometer detector (QqQ, 6420 Triple Quad/LC MS, Agilent Technologies) equipped with a TurboIonSpray source operating in negative-ion mode.

    Techniques: Ion Chromatography, Liquid Chromatography, Mass Spectrometry

    Addition of the IMS dimension enables better quantitation of MS1 data. Calibration curves for 10 of the BSA target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Addition of the IMS dimension enables better quantitation of MS1 data. Calibration curves for 10 of the BSA target peptides spiked into yeast and extracted in the MS1 spectra either A ) without or B ) with IMS filtering. IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise in the monoisotopic and subsequent isotopic peaks. A specific example of interference is illustrated in the bottom XICs for the second isotope of MPCTEDYSLILNR 3+ in the 10 nM BSA/yeast sample, where the red peak appears to have a shoulder at an earlier retention time when IMS filtering was not applied.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Quantitation Assay

    Interferences increase at lower concentrations in the all-ions fragmentation spectra when IMS filtering is not utilized. LC-CID-MS spectra for the transition ions of SLHTLFGDELC[+57]K 2+ are shown at 100 pM A ) without and B ) with IMS filtering. The plots on the right of each XIC show two ions co-eluting in the LC dimension, but being separated by IMS. The transition of interest occurs at 29 ms, while the interfering ion is at 33 ms, causing noise around the transitions if IMS filtering is not utilized.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Interferences increase at lower concentrations in the all-ions fragmentation spectra when IMS filtering is not utilized. LC-CID-MS spectra for the transition ions of SLHTLFGDELC[+57]K 2+ are shown at 100 pM A ) without and B ) with IMS filtering. The plots on the right of each XIC show two ions co-eluting in the LC dimension, but being separated by IMS. The transition of interest occurs at 29 ms, while the interfering ion is at 33 ms, causing noise around the transitions if IMS filtering is not utilized.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Mass Spectrometry

    Precursor and fragment ions have the same IMS drift times in the IMS-MS and IMS-CID-MS spectra. Plots for 5 nM BSA spiked into yeast illustrating the A ) IMS-MS and B ) IMS-CID-MS spectra taken consecutively at 17.74 and 17.75 min. The fragment ions in B ) can be observed at the same drift times as their precursors in A ) due to IMS separation followed by CID. Two different regions are highlighted illustrated the precursors and corresponding fragments.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Precursor and fragment ions have the same IMS drift times in the IMS-MS and IMS-CID-MS spectra. Plots for 5 nM BSA spiked into yeast illustrating the A ) IMS-MS and B ) IMS-CID-MS spectra taken consecutively at 17.74 and 17.75 min. The fragment ions in B ) can be observed at the same drift times as their precursors in A ) due to IMS separation followed by CID. Two different regions are highlighted illustrated the precursors and corresponding fragments.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Mass Spectrometry

    Addition of the IMS dimension enables better quantitation in fragmentation data. Calibration curves for 10 BSA target peptides spiked into yeast and extracted in the A ) LC-CID-MS and B ) LC-IMS-CID-MS all-ions fragmentation spectra. Like the MS1 spectra, IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise. A specific example of interference is illustrated in the bottom XICs for the baseline area around the transitions of YiC[+57]DNQDTISSK + in the 10 nM BSA/yeast sample.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Using Skyline to Analyze Data Containing Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Dimensions

    doi: 10.1007/s13361-018-2028-5

    Figure Lengend Snippet: Addition of the IMS dimension enables better quantitation in fragmentation data. Calibration curves for 10 BSA target peptides spiked into yeast and extracted in the A ) LC-CID-MS and B ) LC-IMS-CID-MS all-ions fragmentation spectra. Like the MS1 spectra, IMS filtering illustrated a greater dynamic range for all peptides due to the reduction of noise. A specific example of interference is illustrated in the bottom XICs for the baseline area around the transitions of YiC[+57]DNQDTISSK + in the 10 nM BSA/yeast sample.

    Article Snippet: To evaluate the effect of utilizing the IMS dimension in multidimensional analyses and determine the performance of Skyline in analyzing the LC-IMS-CID-MS data, tryptically digested BSA was spiked at seven concentrations (100 pM, 1 nM, 5 nM, 10 nM, 100 nM, 500 nM, and 1 μM) into a tryptic yeast digest with a final peptide concentration of 0.1 μg/μL.

    Techniques: Quantitation Assay, Mass Spectrometry

    Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by nano-LC Chip Q-ToF MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p

    Journal: Catalysts (Basel, Switzerland)

    Article Title: Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans

    doi: 10.3390/catal8070278

    Figure Lengend Snippet: Mean relative quantities of various classes of released N -glycans from bovine colostrum whey proteins determined by nano-LC Chip Q-ToF MS/MS by free and immobilized Endo BI-1. Error bars represent one standard deviation. * Represents significant differences within the same class of N -glycans at p

    Article Snippet: N-glycan Analysis by Mass Spectrometry Nano-LC-Chip Q ToF Samples were transferred to polypropylene vials and 6 μL were injected into an Agilent 6520 nano-LC-Chip quadrupole time-of-flight mass spectrometer (Q-ToF MS, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Chromatin Immunoprecipitation, Mass Spectrometry, Standard Deviation