liquid chromatography electrospray ionization tandem mass spectrometry  (Shimadzu Corporation)

 
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    Structured Review

    Shimadzu Corporation liquid chromatography electrospray ionization tandem mass spectrometry
    Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography electrospray ionization tandem mass spectrometry/product/Shimadzu Corporation
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography electrospray ionization tandem mass spectrometry - by Bioz Stars, 2020-09
    90/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Chain length specificity for activation of cPLA2? by C1P: use of the dodecane delivery system to determine lipid-specific effects
    Article Snippet: .. The lipids were extracted as described by Merrill et al. ( ) and quantified using liquid chromatography electrospray ionization tandem mass spectrometry using a Shimadzu HPLC system coupled to a 4000 QTRAP mass spectrometer (Applied Biosystems) as described ( ). ..

    Chromatography:

    Article Title: Identification and Validation of a Salivary Protein Panel to Detect Heart Failure Early
    Article Snippet: .. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-MS/MS) was performed using a Prominence nanoLC system (Shimadzu, Kyoto, Japan) coupled to a TripleTof 5600 mass spectrometer with a Nanospray III interface (SCIEX, Framingham, MA, USA) essentially as previously described . .. In short, approximately 2 µg of peptides were injected and separated on a Vydac EVEREST reversed-phase C18 HPLC column (300 Å pore size, 5 μm particle size, 150 μm i.d.

    Article Title: Biodiversity of β-Carboline Profile of Banisteriopsis caapi and Ayahuasca, a Plant and a Brew with Neuropharmacological Potential
    Article Snippet: .. Analysis of the β-carbolines and DMT by Liquid Chromatography–Electrospray Ionization-Tandem Mass Spectrometry (LC-MS/MS) LC-MS/MS analysis was performed using a Shimadzu LC system (Shimadzu, Kyoto, Japan) coupled to a 4000 Qtrap triple-quadrupole mass spectrometer (Sciex, Framingham, MA, USA), fitted with a Turbo Ion Spray electrospray ionization source (ESI). .. System operation and data acquisition were controlled by Analyst® (V 1.5.2) software (Sciex).The analyte-dependent MS/MS parameters were optimized by direct infusion of a solution containing all the analytes (10 ng/mL), and the analysis was performed in multiple reaction monitoring (MRM) positive mode.

    Article Title: Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
    Article Snippet: .. Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) The identification of flavonoids and other phenolic compounds was conducted in the liquid chromatography (LC) (Prominence model, Shimadzu, Japan) linked to a mass spectrometer ion trap (Esquire HCT model, Bruker Daltonics, Germany) and electrospray ionization interface (ESI). .. The separation conditions were the same as those used for the HPLC-DAD, described in section High-Performance Liquid Chromatography With Diode Array Detector (HPLC-DAD).

    Article Title: A Paclitaxel-Loaded Recombinant Polypeptide Nanoparticle Outperforms Abraxane in Multiple Murine Cancer Models
    Article Snippet: .. All measurements were performed on a liquid chromatography/electrospray ionization/tandem-mass spectrometry (LC-ESI-MS/MS) instrument consisting of a Shimadzu 20A series liquid chromatography (LC) system and an Applied Biosystems MDS Sciex 4000 QTrap tandem-mass spectrometer with electrospray ionization (ESI-MS/MS) operated in multiple reactions monitoring (MRM) mode within Analyst v. 1.4.2 software. .. CP-PTX sample preparation: Aliquots of 5 µL of plasma or 30 µL of tissue homogenate (1 part tissue (grams) + 2 parts deionized water (mL) were mixed with 10 µL of the PTX-d5 (internal standard) and 50 µL of 500 µg/mL freshly prepared proteinase K in Tris buffer pH 7.4, incubated at 55°C for 3 h, and then diluted with 100 µL of deionized water.

    Article Title: Sphingomyelin Synthase 2 Inhibition Ameliorates Cerebral Ischemic Reperfusion Injury Through Reducing the Recruitment of Toll‐Like Receptor 4 to Lipid Rafts
    Article Snippet: .. Sphingomyelin Quantification by Liquid Chromatography/Tandem Mass Spectrometry Brain tissue was homogenized and sphingomyelin was extracted using an alkaline MTBE method as previously described., Liquid chromatography/electrospray ionization/tandem mass spectrometry was performed using a Shimadzu LC‐20 AD binary pump system coupled to a SIL‐20AC autoinjector and DGU20A3 degasser interfaced with an ABI 4000 QTrap mass spectrometer (Applied Biosystems Division, Life Technologies, Carlsbad, CA) equipped with an electrospray ionization source. .. Chromatographic separations were carried out on a Waters Symmetry C18 column (3.5 μm, 2.1 mm i.d.×100 mm) with a Waters C18 guard column (3.5 μm, 2.1 mm i.d.×10 mm; Waters Corporation, Milford, MA).

    Liquid Chromatography:

    Article Title: Chain length specificity for activation of cPLA2? by C1P: use of the dodecane delivery system to determine lipid-specific effects
    Article Snippet: .. The lipids were extracted as described by Merrill et al. ( ) and quantified using liquid chromatography electrospray ionization tandem mass spectrometry using a Shimadzu HPLC system coupled to a 4000 QTRAP mass spectrometer (Applied Biosystems) as described ( ). ..

    Article Title: Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
    Article Snippet: .. Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) The identification of flavonoids and other phenolic compounds was conducted in the liquid chromatography (LC) (Prominence model, Shimadzu, Japan) linked to a mass spectrometer ion trap (Esquire HCT model, Bruker Daltonics, Germany) and electrospray ionization interface (ESI). .. The separation conditions were the same as those used for the HPLC-DAD, described in section High-Performance Liquid Chromatography With Diode Array Detector (HPLC-DAD).

    Article Title: A Paclitaxel-Loaded Recombinant Polypeptide Nanoparticle Outperforms Abraxane in Multiple Murine Cancer Models
    Article Snippet: .. All measurements were performed on a liquid chromatography/electrospray ionization/tandem-mass spectrometry (LC-ESI-MS/MS) instrument consisting of a Shimadzu 20A series liquid chromatography (LC) system and an Applied Biosystems MDS Sciex 4000 QTrap tandem-mass spectrometer with electrospray ionization (ESI-MS/MS) operated in multiple reactions monitoring (MRM) mode within Analyst v. 1.4.2 software. .. CP-PTX sample preparation: Aliquots of 5 µL of plasma or 30 µL of tissue homogenate (1 part tissue (grams) + 2 parts deionized water (mL) were mixed with 10 µL of the PTX-d5 (internal standard) and 50 µL of 500 µg/mL freshly prepared proteinase K in Tris buffer pH 7.4, incubated at 55°C for 3 h, and then diluted with 100 µL of deionized water.

    Mass Spectrometry:

    Article Title: Chain length specificity for activation of cPLA2? by C1P: use of the dodecane delivery system to determine lipid-specific effects
    Article Snippet: .. The lipids were extracted as described by Merrill et al. ( ) and quantified using liquid chromatography electrospray ionization tandem mass spectrometry using a Shimadzu HPLC system coupled to a 4000 QTRAP mass spectrometer (Applied Biosystems) as described ( ). ..

    Article Title: Identification and Validation of a Salivary Protein Panel to Detect Heart Failure Early
    Article Snippet: .. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-MS/MS) was performed using a Prominence nanoLC system (Shimadzu, Kyoto, Japan) coupled to a TripleTof 5600 mass spectrometer with a Nanospray III interface (SCIEX, Framingham, MA, USA) essentially as previously described . .. In short, approximately 2 µg of peptides were injected and separated on a Vydac EVEREST reversed-phase C18 HPLC column (300 Å pore size, 5 μm particle size, 150 μm i.d.

    Article Title: Biodiversity of β-Carboline Profile of Banisteriopsis caapi and Ayahuasca, a Plant and a Brew with Neuropharmacological Potential
    Article Snippet: .. Analysis of the β-carbolines and DMT by Liquid Chromatography–Electrospray Ionization-Tandem Mass Spectrometry (LC-MS/MS) LC-MS/MS analysis was performed using a Shimadzu LC system (Shimadzu, Kyoto, Japan) coupled to a 4000 Qtrap triple-quadrupole mass spectrometer (Sciex, Framingham, MA, USA), fitted with a Turbo Ion Spray electrospray ionization source (ESI). .. System operation and data acquisition were controlled by Analyst® (V 1.5.2) software (Sciex).The analyte-dependent MS/MS parameters were optimized by direct infusion of a solution containing all the analytes (10 ng/mL), and the analysis was performed in multiple reaction monitoring (MRM) positive mode.

    Article Title: Effect of Quercetin Rich Onion Extracts on Bacterial Quorum Sensing
    Article Snippet: .. Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) The identification of flavonoids and other phenolic compounds was conducted in the liquid chromatography (LC) (Prominence model, Shimadzu, Japan) linked to a mass spectrometer ion trap (Esquire HCT model, Bruker Daltonics, Germany) and electrospray ionization interface (ESI). .. The separation conditions were the same as those used for the HPLC-DAD, described in section High-Performance Liquid Chromatography With Diode Array Detector (HPLC-DAD).

    Article Title: Sphingomyelin Synthase 2 Inhibition Ameliorates Cerebral Ischemic Reperfusion Injury Through Reducing the Recruitment of Toll‐Like Receptor 4 to Lipid Rafts
    Article Snippet: .. Sphingomyelin Quantification by Liquid Chromatography/Tandem Mass Spectrometry Brain tissue was homogenized and sphingomyelin was extracted using an alkaline MTBE method as previously described., Liquid chromatography/electrospray ionization/tandem mass spectrometry was performed using a Shimadzu LC‐20 AD binary pump system coupled to a SIL‐20AC autoinjector and DGU20A3 degasser interfaced with an ABI 4000 QTrap mass spectrometer (Applied Biosystems Division, Life Technologies, Carlsbad, CA) equipped with an electrospray ionization source. .. Chromatographic separations were carried out on a Waters Symmetry C18 column (3.5 μm, 2.1 mm i.d.×100 mm) with a Waters C18 guard column (3.5 μm, 2.1 mm i.d.×10 mm; Waters Corporation, Milford, MA).

    Software:

    Article Title: A Paclitaxel-Loaded Recombinant Polypeptide Nanoparticle Outperforms Abraxane in Multiple Murine Cancer Models
    Article Snippet: .. All measurements were performed on a liquid chromatography/electrospray ionization/tandem-mass spectrometry (LC-ESI-MS/MS) instrument consisting of a Shimadzu 20A series liquid chromatography (LC) system and an Applied Biosystems MDS Sciex 4000 QTrap tandem-mass spectrometer with electrospray ionization (ESI-MS/MS) operated in multiple reactions monitoring (MRM) mode within Analyst v. 1.4.2 software. .. CP-PTX sample preparation: Aliquots of 5 µL of plasma or 30 µL of tissue homogenate (1 part tissue (grams) + 2 parts deionized water (mL) were mixed with 10 µL of the PTX-d5 (internal standard) and 50 µL of 500 µg/mL freshly prepared proteinase K in Tris buffer pH 7.4, incubated at 55°C for 3 h, and then diluted with 100 µL of deionized water.

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    Shimadzu Corporation esi ms ms analysis
    Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by <t>HILIC-LC-ESI-MS/MS.</t> (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.
    Esi Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Shimadzu Corporation uhplc esi ms ms analyses
    Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by <t>UHPLC-ESI-MS/MS</t> chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.
    Uhplc Esi Ms Ms Analyses, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uhplc esi ms ms analyses/product/Shimadzu Corporation
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    88
    Shimadzu Corporation electrospray ionization esi liquid chromatography lc ms ms
    Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by <t>electrospray</t> ionization <t>(ESI)</t> liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p
    Electrospray Ionization Esi Liquid Chromatography Lc Ms Ms, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray ionization esi liquid chromatography lc ms ms/product/Shimadzu Corporation
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Shimadzu Corporation hplc esi ms ms analysis
    Total ion chromatorgraphies of CS aqueous extract by <t>HPLC-ESI-MS/MS</t> analysis. (a) Positive ion mode. (b) Negative ion mode.
    Hplc Esi Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc esi ms ms analysis/product/Shimadzu Corporation
    Average 90 stars, based on 3 article reviews
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    Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by HILIC-LC-ESI-MS/MS. (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.

    Journal: PLoS ONE

    Article Title: Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi

    doi: 10.1371/journal.pone.0184953

    Figure Lengend Snippet: Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by HILIC-LC-ESI-MS/MS. (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.

    Article Snippet: HILIC-LC-ESI-MS/MS ESI-MS/MS analysis was performed on a Shimadzu (Kyoto, Japan) LC-ESI-MS-IT-TOF operating in the negative ion mode.

    Techniques: Activity Assay, Recombinant, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Acetylene Reduction Assay, Plasmid Preparation, Diagnostic Assay, Amplification

    Detection of bUAS transcripts and UDP-apiose. In vivo indication for the functional activity of bUAS genes and enzymes. (A) RT-PCR analyses showing bUAS transcripts of G . roseus and X . pisi . (B) HILIC-LC-ESI-MS/MS analysis of aq-methanolic (MeOH:chloroform:H 2 O; 40:40:20, v/v/v) extracts. Negative mode [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line; amplified by a factor of 10), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed.

    Journal: PLoS ONE

    Article Title: Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi

    doi: 10.1371/journal.pone.0184953

    Figure Lengend Snippet: Detection of bUAS transcripts and UDP-apiose. In vivo indication for the functional activity of bUAS genes and enzymes. (A) RT-PCR analyses showing bUAS transcripts of G . roseus and X . pisi . (B) HILIC-LC-ESI-MS/MS analysis of aq-methanolic (MeOH:chloroform:H 2 O; 40:40:20, v/v/v) extracts. Negative mode [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line; amplified by a factor of 10), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed.

    Article Snippet: HILIC-LC-ESI-MS/MS ESI-MS/MS analysis was performed on a Shimadzu (Kyoto, Japan) LC-ESI-MS-IT-TOF operating in the negative ion mode.

    Techniques: In Vivo, Functional Assay, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Diagnostic Assay, Amplification

    Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by UHPLC-ESI-MS/MS chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.

    Journal: Molecules

    Article Title: Antidepressant Potential of Chlorogenic Acid-Enriched Extract from Eucommia ulmoides Oliver Bark with Neuron Protection and Promotion of Serotonin Release through Enhancing Synapsin I Expression

    doi: 10.3390/molecules21030260

    Figure Lengend Snippet: Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by UHPLC-ESI-MS/MS chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.

    Article Snippet: UHPLC-ESI-MS/MS Analysis The UHPLC-ESI-MS/MS analyses were performed on a Shimadzu LCMS-8040 UHPLC system comprised of two LC-30AD pumps, a SIL-30AC autosampler with a CTO-30AC column oven, a DGU-20A5 degasser, a Shimadzu CBM-20A system controller, a Labsolution LCMS Ver.5.75 workstation, an ESI ion source and a LCMS-8040 mass spectrometer.

    Techniques: Mass Spectrometry

    UHPLC-ESI-MS/MS chromatogram of CGA and toosendanin (TSN). ( A ) Negative Scan m / z 100–500 of CGA; ( B ) MS/MS spectrum of m / z 353.10 in the negative ion mode; ( C ) Negative Scan m / z 100–700 of TSN; ( D ) MS/MS spectrum of m / z 573.10 in the negative ion mode; ( E ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for blank CSF sample; ( F ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for CSF spiked with CGA; ( G ) MRM of channels ESI − 573.15/531.15 for blank CSF sample; ( H ) MRM of channels ESI − 573.15/531.15 for CSF spiked with TSN. Notes: Chromatographic conditions are provided in the Experimental Section.

    Journal: Molecules

    Article Title: Antidepressant Potential of Chlorogenic Acid-Enriched Extract from Eucommia ulmoides Oliver Bark with Neuron Protection and Promotion of Serotonin Release through Enhancing Synapsin I Expression

    doi: 10.3390/molecules21030260

    Figure Lengend Snippet: UHPLC-ESI-MS/MS chromatogram of CGA and toosendanin (TSN). ( A ) Negative Scan m / z 100–500 of CGA; ( B ) MS/MS spectrum of m / z 353.10 in the negative ion mode; ( C ) Negative Scan m / z 100–700 of TSN; ( D ) MS/MS spectrum of m / z 573.10 in the negative ion mode; ( E ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for blank CSF sample; ( F ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for CSF spiked with CGA; ( G ) MRM of channels ESI − 573.15/531.15 for blank CSF sample; ( H ) MRM of channels ESI − 573.15/531.15 for CSF spiked with TSN. Notes: Chromatographic conditions are provided in the Experimental Section.

    Article Snippet: UHPLC-ESI-MS/MS Analysis The UHPLC-ESI-MS/MS analyses were performed on a Shimadzu LCMS-8040 UHPLC system comprised of two LC-30AD pumps, a SIL-30AC autosampler with a CTO-30AC column oven, a DGU-20A5 degasser, a Shimadzu CBM-20A system controller, a Labsolution LCMS Ver.5.75 workstation, an ESI ion source and a LCMS-8040 mass spectrometer.

    Techniques: Mass Spectrometry

    Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by electrospray ionization (ESI) liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p

    Journal: PLoS ONE

    Article Title: Chronic Running Exercise Alleviates Early Progression of Nephropathy with Upregulation of Nitric Oxide Synthases and Suppression of Glycation in Zucker Diabetic Rats

    doi: 10.1371/journal.pone.0138037

    Figure Lengend Snippet: Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by electrospray ionization (ESI) liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p

    Article Snippet: Quantitative analysis of the derivatized α-oxoaldehydes by electrospray ionization (ESI) liquid chromatography (LC)-MS/MS was performed with a Nexera Quanternary LC Systems (Shimadzu Co., Tokyo, Japan) coupled to a TSQ Quantum Ultra (Thermo Fisher Scientific) and operated in the positive ion mode.

    Techniques: Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Total ion chromatorgraphies of CS aqueous extract by HPLC-ESI-MS/MS analysis. (a) Positive ion mode. (b) Negative ion mode.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin

    doi: 10.1155/2016/9623957

    Figure Lengend Snippet: Total ion chromatorgraphies of CS aqueous extract by HPLC-ESI-MS/MS analysis. (a) Positive ion mode. (b) Negative ion mode.

    Article Snippet: HPLC-ESI-MS/MS Analysis of CS Aqueous Extract HPLC analysis was executed on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AD pump, a SIL-20AC automatic sampler, and CTO-20A thermostatic column compartment (Shimadzu Kyoto, Japan).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry