liquid chromatography electrospray ionization tandem mass spectrometry  (SCIEX)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    SCIEX liquid chromatography electrospray ionization tandem mass spectrometry
    Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography electrospray ionization tandem mass spectrometry/product/SCIEX
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography electrospray ionization tandem mass spectrometry - by Bioz Stars, 2021-06
    86/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: Pharmacokinetics and pharmacodynamics of a fixed-dose combination of gemigliptin/metformin sustained release 25/500 mg compared to the loose combination in healthy male subjects
    Article Snippet: .. Determination of plasma concentrations of gemigliptin and metformin and plasma DPP-4 activity The plasma concentrations of gemigliptin and LC15-0636 were determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) (API™ 5500 UFLC system; SCIEX, Seoul, Korea) with electrospray in positive ionization mode. .. For gemigliptin and LC15-0636, the chromatographic separation was performed at 40°C using a Unison UK-C18 (50 × 2.0 mm, 3 μm; Shimadzu, Kyoto, Japan).

    Liquid Chromatography:

    Article Title: Pharmacokinetics and pharmacodynamics of a fixed-dose combination of gemigliptin/metformin sustained release 25/500 mg compared to the loose combination in healthy male subjects
    Article Snippet: .. Determination of plasma concentrations of gemigliptin and metformin and plasma DPP-4 activity The plasma concentrations of gemigliptin and LC15-0636 were determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) (API™ 5500 UFLC system; SCIEX, Seoul, Korea) with electrospray in positive ionization mode. .. For gemigliptin and LC15-0636, the chromatographic separation was performed at 40°C using a Unison UK-C18 (50 × 2.0 mm, 3 μm; Shimadzu, Kyoto, Japan).

    Article Title: Incorporation of iloprost in phospholipase-resistant phospholipid scaffold enhances its barrier protective effects on pulmonary endothelium
    Article Snippet: .. UHPLC-ESI-MS/MS analysis of iloprost and iloprost-PC The content of iloprost and iloprost-PC were determined by liquid chromatography electrospray ionization tandem mass spectrometry using Sciex 6500QTRAP mass spectrometer coupled with Shimadzu Nexera X2 UHPLC system. ..

    Mass Spectrometry:

    Article Title: Pharmacokinetics and pharmacodynamics of a fixed-dose combination of gemigliptin/metformin sustained release 25/500 mg compared to the loose combination in healthy male subjects
    Article Snippet: .. Determination of plasma concentrations of gemigliptin and metformin and plasma DPP-4 activity The plasma concentrations of gemigliptin and LC15-0636 were determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) (API™ 5500 UFLC system; SCIEX, Seoul, Korea) with electrospray in positive ionization mode. .. For gemigliptin and LC15-0636, the chromatographic separation was performed at 40°C using a Unison UK-C18 (50 × 2.0 mm, 3 μm; Shimadzu, Kyoto, Japan).

    Article Title: Lipid-Induced Epigenomic Changes in Human Macrophages Identify a Coronary Artery Disease-Associated Variant that Regulates PPAP2B Expression through Altered C/EBP-Beta Binding
    Article Snippet: Measurement of LPP3 substrates and products Cholesterol, cholesterol ester and a series of LPP3 substrates and their cognate dephosphorylation products were quantitated in macrophage and foam cell lipid extracts by electrospray ionization tandem mass spectrometry using methods that have been described previously [ , ]. .. Proteomic quantitation of LPP3 LPP3-derived tryptic peptides were quantitated using capillary flow reverse phase HPLC and electrospray ionization tandem mass spectrometry using an AB Sciex 5600 Q-TOF mass spectrometer (AB SCIEX, Framingham, MA) operated in high-resolution selected ion monitoring mode. .. We quantitated the following peptides STIQNPYVAALYK, NGGSPALNNNPR, EILSPVDIIDR and used a mass-labelled derivative of the latter of these (New England Peptides, Gardner, MA) as an internal standard [ ].

    Article Title: Dysregulation of lysophosphatidic acids in multiple sclerosis and autoimmune encephalomyelitis
    Article Snippet: .. Analysis of lysophosphatidic acids Quantitative LPA analysis was performed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a hybrid triple quadrupole-ion trap QTrap 5500 mass spectrometer (AB Sciex, Germany) as described [ , ] without knowledge of clinical data or treatment groups. ..

    Article Title: Incorporation of iloprost in phospholipase-resistant phospholipid scaffold enhances its barrier protective effects on pulmonary endothelium
    Article Snippet: .. UHPLC-ESI-MS/MS analysis of iloprost and iloprost-PC The content of iloprost and iloprost-PC were determined by liquid chromatography electrospray ionization tandem mass spectrometry using Sciex 6500QTRAP mass spectrometer coupled with Shimadzu Nexera X2 UHPLC system. ..

    Article Title: Sphingosine-1-phosphate Signalling drives an Angiogenic Transcriptional Programme in Diffuse Large B Cell Lymphoma
    Article Snippet: Supernatants were harvested into pre-chilled HPLC grade methanol (Sigma-Aldrich) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific). .. S1P levels were quantified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS; 4000 QTRAP, AB Sciex, Framingham, MA, USA) as previously described [ ]. .. Treatment of cells S1P (Sigma-Aldrich) was prepared as in and as before [ ].

    Article Title: A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell-dependent mouse model of allergic asthma
    Article Snippet: Mucin in BAL fluid was measured using a previously described ELISA with mouse Muc5AC-specific antibody (Pierce, Rockford, IL) . .. Lipids were extracted from lung tissues and serum, and S1P, dihydro-S1P, and SK1-I were measured by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS; 4000 QTRAP; AB Sciex, Foster City, CA), as described previously . ..

    Article Title: Comparative analysis of obesity-related cardiometabolic and renal biomarkers in human plasma and serum
    Article Snippet: Analytes were separated using a Waters Acquity ultra-performance liquid chromatography (UPLC; Waters, Milford, MA) on a 2.1 mm × 150 mm, 1.7 µm BEH C18 column (Waters) for analysis of oxylipins and endocannabinoids, and 2.1 mm × 150 mm, 1.7 µm BEH C8 column (Waters) for analysis of ceramides. .. Separated analytes were detected by tandem mass-spectrometry, using electrospray ionization with multi reaction monitoring on an API 6500 QTRAP (Sciex, Redwood City, CA) for oxylipins and endocannabinoids, and an API 4000 QTRAP (Sciex) for ceramides. ..

    Article Title: Pulmonary Metabolism of Resveratrol: In Vitro and In Vivo Evidence
    Article Snippet: RES, R3S, and R3G plasma protein binding was determined at a concentration of 20 µ M. The protein binding assay was performed with a published protocol ( ). .. RES, R3S, and R3G concentrations in plasma and in the in vitro reaction mixture were measured with an electrospray ionization liquid chromatography-tandem mass spectrometry system (ABSciex API 4000; Framingham, MA) set in negative ion scan mode as described previously ( ). ..

    Quantitation Assay:

    Article Title: Lipid-Induced Epigenomic Changes in Human Macrophages Identify a Coronary Artery Disease-Associated Variant that Regulates PPAP2B Expression through Altered C/EBP-Beta Binding
    Article Snippet: Measurement of LPP3 substrates and products Cholesterol, cholesterol ester and a series of LPP3 substrates and their cognate dephosphorylation products were quantitated in macrophage and foam cell lipid extracts by electrospray ionization tandem mass spectrometry using methods that have been described previously [ , ]. .. Proteomic quantitation of LPP3 LPP3-derived tryptic peptides were quantitated using capillary flow reverse phase HPLC and electrospray ionization tandem mass spectrometry using an AB Sciex 5600 Q-TOF mass spectrometer (AB SCIEX, Framingham, MA) operated in high-resolution selected ion monitoring mode. .. We quantitated the following peptides STIQNPYVAALYK, NGGSPALNNNPR, EILSPVDIIDR and used a mass-labelled derivative of the latter of these (New England Peptides, Gardner, MA) as an internal standard [ ].

    Flow Cytometry:

    Article Title: Lipid-Induced Epigenomic Changes in Human Macrophages Identify a Coronary Artery Disease-Associated Variant that Regulates PPAP2B Expression through Altered C/EBP-Beta Binding
    Article Snippet: Measurement of LPP3 substrates and products Cholesterol, cholesterol ester and a series of LPP3 substrates and their cognate dephosphorylation products were quantitated in macrophage and foam cell lipid extracts by electrospray ionization tandem mass spectrometry using methods that have been described previously [ , ]. .. Proteomic quantitation of LPP3 LPP3-derived tryptic peptides were quantitated using capillary flow reverse phase HPLC and electrospray ionization tandem mass spectrometry using an AB Sciex 5600 Q-TOF mass spectrometer (AB SCIEX, Framingham, MA) operated in high-resolution selected ion monitoring mode. .. We quantitated the following peptides STIQNPYVAALYK, NGGSPALNNNPR, EILSPVDIIDR and used a mass-labelled derivative of the latter of these (New England Peptides, Gardner, MA) as an internal standard [ ].

    High Performance Liquid Chromatography:

    Article Title: Lipid-Induced Epigenomic Changes in Human Macrophages Identify a Coronary Artery Disease-Associated Variant that Regulates PPAP2B Expression through Altered C/EBP-Beta Binding
    Article Snippet: Measurement of LPP3 substrates and products Cholesterol, cholesterol ester and a series of LPP3 substrates and their cognate dephosphorylation products were quantitated in macrophage and foam cell lipid extracts by electrospray ionization tandem mass spectrometry using methods that have been described previously [ , ]. .. Proteomic quantitation of LPP3 LPP3-derived tryptic peptides were quantitated using capillary flow reverse phase HPLC and electrospray ionization tandem mass spectrometry using an AB Sciex 5600 Q-TOF mass spectrometer (AB SCIEX, Framingham, MA) operated in high-resolution selected ion monitoring mode. .. We quantitated the following peptides STIQNPYVAALYK, NGGSPALNNNPR, EILSPVDIIDR and used a mass-labelled derivative of the latter of these (New England Peptides, Gardner, MA) as an internal standard [ ].

    Chromatography:

    Article Title: Dysregulation of lysophosphatidic acids in multiple sclerosis and autoimmune encephalomyelitis
    Article Snippet: .. Analysis of lysophosphatidic acids Quantitative LPA analysis was performed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a hybrid triple quadrupole-ion trap QTrap 5500 mass spectrometer (AB Sciex, Germany) as described [ , ] without knowledge of clinical data or treatment groups. ..

    Article Title: Sphingosine-1-phosphate Signalling drives an Angiogenic Transcriptional Programme in Diffuse Large B Cell Lymphoma
    Article Snippet: Supernatants were harvested into pre-chilled HPLC grade methanol (Sigma-Aldrich) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific). .. S1P levels were quantified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS; 4000 QTRAP, AB Sciex, Framingham, MA, USA) as previously described [ ]. .. Treatment of cells S1P (Sigma-Aldrich) was prepared as in and as before [ ].

    Article Title: A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell-dependent mouse model of allergic asthma
    Article Snippet: Mucin in BAL fluid was measured using a previously described ELISA with mouse Muc5AC-specific antibody (Pierce, Rockford, IL) . .. Lipids were extracted from lung tissues and serum, and S1P, dihydro-S1P, and SK1-I were measured by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS; 4000 QTRAP; AB Sciex, Foster City, CA), as described previously . ..

    Article Title: Pulmonary Metabolism of Resveratrol: In Vitro and In Vivo Evidence
    Article Snippet: RES, R3S, and R3G plasma protein binding was determined at a concentration of 20 µ M. The protein binding assay was performed with a published protocol ( ). .. RES, R3S, and R3G concentrations in plasma and in the in vitro reaction mixture were measured with an electrospray ionization liquid chromatography-tandem mass spectrometry system (ABSciex API 4000; Framingham, MA) set in negative ion scan mode as described previously ( ). ..

    In Vitro:

    Article Title: Pulmonary Metabolism of Resveratrol: In Vitro and In Vivo Evidence
    Article Snippet: RES, R3S, and R3G plasma protein binding was determined at a concentration of 20 µ M. The protein binding assay was performed with a published protocol ( ). .. RES, R3S, and R3G concentrations in plasma and in the in vitro reaction mixture were measured with an electrospray ionization liquid chromatography-tandem mass spectrometry system (ABSciex API 4000; Framingham, MA) set in negative ion scan mode as described previously ( ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    SCIEX electrospray quadrupole time of flight mass spectrometer
    Molecular weight determinations of the bioactive protein MULTIFUNCin and its native form. (A) A matrix-assisted laser desorption ionization time-of-flight (MALDI-ToF) mass spectrum shows abundant peaks for the singly- and doubly-charged MULTIFUNCin ions corresponding to a molecular mass of 199.6 kDa in the intact molecule. (B) <t>Electrospray-ionization</t> gas-phase electrophoretic mobility molecular analysis (ESI-GEMMA) of the active LCA fraction (glycopeak) under native conditions (in 20 mmol L -1 ammonium acetate at pH 8.0). An abundant peak with an EMD of 12.9 nm indicates a molecular mass of ∼ 390 kDa (± 5%), thus characterizing MULTIFUNCin as a homodimeric protein.
    Electrospray Quadrupole Time Of Flight Mass Spectrometer, supplied by SCIEX, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray quadrupole time of flight mass spectrometer/product/SCIEX
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    electrospray quadrupole time of flight mass spectrometer - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    99
    SCIEX protein esi
    Whole protein <t>ESI-MS</t> and LC-MS/MS characterization of <t>HMGB1</t> isoforms in healthy volunteers and in patients with ALD. Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from serum of healthy volunteers ( A ) or patients with ALD ( B ) are shown. Molecular weights and a schematic representation of each isoform are indicated on each spectra where required. Representative spectra of the tandem MS characterization of a peptide containing lysine residues (Lys-28, -29, and -30) within NLS1 ( C ) and lysine residues (Lys-180, -182, -183, -184, and -185) within NLS2 ( D ) after Glu-C digestion of the 25,467- and 25,549-Da human HMGB1 isoforms following extraction from the serum of ALD patients are shown. HMGB1 was enzymatically cleaved with endopeptidase Glu-C to confirm the presence or absence of acetyl modifications on specific lysine residues (shown as K(Ac) ) as described under “Experimental Procedures.” The one-letter amino acid code is given for each peptide sequence, and b and y ions are labeled with molecular weights and amino acid code where appropriate. Data are representative of either 10 healthy volunteers or 10 ALD patients.
    Protein Esi, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein esi/product/SCIEX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein esi - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    99
    SCIEX esi q tof ms
    A , lipid species in gastrocnemius identified by global lipidomics. Number of lipid species by category identified in a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to <t>ESI-Q-TOF-MS</t> in the lipid extract of gastrocnemius of LDLRKO mice after normal (NS; n = 7) or low-sodium diet (LS; n = 7). 1G-Cer, glycosyl ceramide; AC, acylcarnitine; Cer, ceramide; CL, cardiolipins; DG, diacylglycerol; FFA, free fatty acids; oPE, alkylsulfatylethylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; pPC, plasmenyl diacylphosphatidylcholine; pPE, plasmenyl phosphattidylethanolamine; Q-9, coenzyme Q9; SM, sphingomyelin; TG, triacylglycerol. B , discriminant analysis by partial least squares (PLS-DA) of lipids in the gastrocnemius. The lipid extract of the gastrocnemius was subjected to a global lipid analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. The PLS-DA shows the separation of lipid species from LDLR KO male mice in NS ( green circles , n = 7) or LS ( red circles , n = 7) groups.
    Esi Q Tof Ms, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi q tof ms/product/SCIEX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    esi q tof ms - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Molecular weight determinations of the bioactive protein MULTIFUNCin and its native form. (A) A matrix-assisted laser desorption ionization time-of-flight (MALDI-ToF) mass spectrum shows abundant peaks for the singly- and doubly-charged MULTIFUNCin ions corresponding to a molecular mass of 199.6 kDa in the intact molecule. (B) Electrospray-ionization gas-phase electrophoretic mobility molecular analysis (ESI-GEMMA) of the active LCA fraction (glycopeak) under native conditions (in 20 mmol L -1 ammonium acetate at pH 8.0). An abundant peak with an EMD of 12.9 nm indicates a molecular mass of ∼ 390 kDa (± 5%), thus characterizing MULTIFUNCin as a homodimeric protein.

    Journal: Integrative and Comparative Biology

    Article Title: MULTIFUNCin: A Multifunctional Protein Cue Induces Habitat Selection by, and Predation on, Barnacles

    doi: 10.1093/icb/icw076

    Figure Lengend Snippet: Molecular weight determinations of the bioactive protein MULTIFUNCin and its native form. (A) A matrix-assisted laser desorption ionization time-of-flight (MALDI-ToF) mass spectrum shows abundant peaks for the singly- and doubly-charged MULTIFUNCin ions corresponding to a molecular mass of 199.6 kDa in the intact molecule. (B) Electrospray-ionization gas-phase electrophoretic mobility molecular analysis (ESI-GEMMA) of the active LCA fraction (glycopeak) under native conditions (in 20 mmol L -1 ammonium acetate at pH 8.0). An abundant peak with an EMD of 12.9 nm indicates a molecular mass of ∼ 390 kDa (± 5%), thus characterizing MULTIFUNCin as a homodimeric protein.

    Article Snippet: Each product was isolated by reversed phase high-performance liquid chromatography (Hewlett-Packard Model 1090; Agilent Technologies, Santa Clara, CA; eluting at 1 ml min − 1 with 100% acetonitrile, 0.1% TFA over 30 min), detected using an electrospray quadrupole Time-of-Flight mass spectrometer (ESI/Q-ToF MS) (QSTAR XL; Applied Biosystems/Sciex, Foster City, CA), and de novo sequenced from the resulting tandem mass spectra ( ).

    Techniques: Molecular Weight, Gas Phase Electrophoretic Molecular Mobility Analysis

    Whole protein ESI-MS and LC-MS/MS characterization of HMGB1 isoforms in healthy volunteers and in patients with ALD. Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from serum of healthy volunteers ( A ) or patients with ALD ( B ) are shown. Molecular weights and a schematic representation of each isoform are indicated on each spectra where required. Representative spectra of the tandem MS characterization of a peptide containing lysine residues (Lys-28, -29, and -30) within NLS1 ( C ) and lysine residues (Lys-180, -182, -183, -184, and -185) within NLS2 ( D ) after Glu-C digestion of the 25,467- and 25,549-Da human HMGB1 isoforms following extraction from the serum of ALD patients are shown. HMGB1 was enzymatically cleaved with endopeptidase Glu-C to confirm the presence or absence of acetyl modifications on specific lysine residues (shown as K(Ac) ) as described under “Experimental Procedures.” The one-letter amino acid code is given for each peptide sequence, and b and y ions are labeled with molecular weights and amino acid code where appropriate. Data are representative of either 10 healthy volunteers or 10 ALD patients.

    Journal: The Journal of Biological Chemistry

    Article Title: High Mobility Group Box-1 (HMGB1) Participates in the Pathogenesis of Alcoholic Liver Disease (ALD) *

    doi: 10.1074/jbc.M114.552141

    Figure Lengend Snippet: Whole protein ESI-MS and LC-MS/MS characterization of HMGB1 isoforms in healthy volunteers and in patients with ALD. Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from serum of healthy volunteers ( A ) or patients with ALD ( B ) are shown. Molecular weights and a schematic representation of each isoform are indicated on each spectra where required. Representative spectra of the tandem MS characterization of a peptide containing lysine residues (Lys-28, -29, and -30) within NLS1 ( C ) and lysine residues (Lys-180, -182, -183, -184, and -185) within NLS2 ( D ) after Glu-C digestion of the 25,467- and 25,549-Da human HMGB1 isoforms following extraction from the serum of ALD patients are shown. HMGB1 was enzymatically cleaved with endopeptidase Glu-C to confirm the presence or absence of acetyl modifications on specific lysine residues (shown as K(Ac) ) as described under “Experimental Procedures.” The one-letter amino acid code is given for each peptide sequence, and b and y ions are labeled with molecular weights and amino acid code where appropriate. Data are representative of either 10 healthy volunteers or 10 ALD patients.

    Article Snippet: Characterization of whole protein molecular weights, acetylated lysine residues, or redox modifications on cysteine residues within HMGB1 was performed as described previously by whole protein ESI or tandem mass spectrometry (MS/MS) ( ) using either an AB Sciex QTRAP 5500 or an AB Sciex TripleTOF 5600 (Sciex Inc., Framingham, MA).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Isolation, Sequencing, Labeling

    Identification of HMGB1 PTMs in serum and liver from control and ethanol-fed mice. Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from either serum ( A ) or liver ( B ) from control mice are shown. Peptide MS of HMGB1 derived from control mouse serum or liver was performed following enzymatic digestion with either Glu-C to confirm acetyl status ( C ) or trypsin to confirm redox status ( D ). Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from either serum ( E ) or liver ( F ) from ethanol-fed mice are shown. Peptide MS of HMGB1 derived from serum or liver from ethanol-fed mice was performed following enzymatic digestion with endopeptidase Glu-C to confirm the lack of acetyl modifications on the 24,587 and 24,585-Da isoforms ( G ) and the presence of acetyl modifications on the 25,467-, 25,469-, and 25,549-Da isoforms ( H ). Molecular weights and a schematic representation of each isoform are indicated on each spectra where required. Acetyl modifications were confirmed on lysine residues (shown as K(Ac) ) after LC-MS/MS analysis for peptides spanning NLS1 (amino acids 27–39) ( I ) and NLS2 (amino acids 180–188 (179–187 minus methionine)) ( J ) in HMGB1 derived from mice fed ethanol. Phosphorylation modifications were confirmed on serine 35 (shown as pS 35 ) after LC-MS/MS analysis of NLS1 ( K ) in HMGB1 derived from mice fed ethanol. The one-letter amino acid code is given for each peptide sequence, and b and y ions are highlighted with molecular weights where appropriate. Data are representative of at least six individual mice per group.

    Journal: The Journal of Biological Chemistry

    Article Title: High Mobility Group Box-1 (HMGB1) Participates in the Pathogenesis of Alcoholic Liver Disease (ALD) *

    doi: 10.1074/jbc.M114.552141

    Figure Lengend Snippet: Identification of HMGB1 PTMs in serum and liver from control and ethanol-fed mice. Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from either serum ( A ) or liver ( B ) from control mice are shown. Peptide MS of HMGB1 derived from control mouse serum or liver was performed following enzymatic digestion with either Glu-C to confirm acetyl status ( C ) or trypsin to confirm redox status ( D ). Representative spectra of whole protein ESI-MS of HMGB1 isoforms isolated from either serum ( E ) or liver ( F ) from ethanol-fed mice are shown. Peptide MS of HMGB1 derived from serum or liver from ethanol-fed mice was performed following enzymatic digestion with endopeptidase Glu-C to confirm the lack of acetyl modifications on the 24,587 and 24,585-Da isoforms ( G ) and the presence of acetyl modifications on the 25,467-, 25,469-, and 25,549-Da isoforms ( H ). Molecular weights and a schematic representation of each isoform are indicated on each spectra where required. Acetyl modifications were confirmed on lysine residues (shown as K(Ac) ) after LC-MS/MS analysis for peptides spanning NLS1 (amino acids 27–39) ( I ) and NLS2 (amino acids 180–188 (179–187 minus methionine)) ( J ) in HMGB1 derived from mice fed ethanol. Phosphorylation modifications were confirmed on serine 35 (shown as pS 35 ) after LC-MS/MS analysis of NLS1 ( K ) in HMGB1 derived from mice fed ethanol. The one-letter amino acid code is given for each peptide sequence, and b and y ions are highlighted with molecular weights where appropriate. Data are representative of at least six individual mice per group.

    Article Snippet: Characterization of whole protein molecular weights, acetylated lysine residues, or redox modifications on cysteine residues within HMGB1 was performed as described previously by whole protein ESI or tandem mass spectrometry (MS/MS) ( ) using either an AB Sciex QTRAP 5500 or an AB Sciex TripleTOF 5600 (Sciex Inc., Framingham, MA).

    Techniques: Mouse Assay, Mass Spectrometry, Isolation, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Sequencing

    A , lipid species in gastrocnemius identified by global lipidomics. Number of lipid species by category identified in a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS in the lipid extract of gastrocnemius of LDLRKO mice after normal (NS; n = 7) or low-sodium diet (LS; n = 7). 1G-Cer, glycosyl ceramide; AC, acylcarnitine; Cer, ceramide; CL, cardiolipins; DG, diacylglycerol; FFA, free fatty acids; oPE, alkylsulfatylethylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; pPC, plasmenyl diacylphosphatidylcholine; pPE, plasmenyl phosphattidylethanolamine; Q-9, coenzyme Q9; SM, sphingomyelin; TG, triacylglycerol. B , discriminant analysis by partial least squares (PLS-DA) of lipids in the gastrocnemius. The lipid extract of the gastrocnemius was subjected to a global lipid analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. The PLS-DA shows the separation of lipid species from LDLR KO male mice in NS ( green circles , n = 7) or LS ( red circles , n = 7) groups.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: A , lipid species in gastrocnemius identified by global lipidomics. Number of lipid species by category identified in a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS in the lipid extract of gastrocnemius of LDLRKO mice after normal (NS; n = 7) or low-sodium diet (LS; n = 7). 1G-Cer, glycosyl ceramide; AC, acylcarnitine; Cer, ceramide; CL, cardiolipins; DG, diacylglycerol; FFA, free fatty acids; oPE, alkylsulfatylethylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; pPC, plasmenyl diacylphosphatidylcholine; pPE, plasmenyl phosphattidylethanolamine; Q-9, coenzyme Q9; SM, sphingomyelin; TG, triacylglycerol. B , discriminant analysis by partial least squares (PLS-DA) of lipids in the gastrocnemius. The lipid extract of the gastrocnemius was subjected to a global lipid analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. The PLS-DA shows the separation of lipid species from LDLR KO male mice in NS ( green circles , n = 7) or LS ( red circles , n = 7) groups.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mass Spectrometry, High Performance Liquid Chromatography, Mouse Assay

    Heat map of lipid species in gastrocnemius. The gastrocnemius lipid extract obtained from normal (NS; n = 7) or low-sodium diet (LS; n = 7) fed LDLR KO male mice was subjected to global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Statistical analysis was performed by using Student's t -test, with “false Discovery rate” (FDR) considering p ≤ 0.05. AC, acylcarnitine; CL, cardiolipins; FFA, free fatty acids; PC, phosphatidylcholine; PI, phosphatidylinositol; pPE, plasmenyl phosphattidylethanolamine.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: Heat map of lipid species in gastrocnemius. The gastrocnemius lipid extract obtained from normal (NS; n = 7) or low-sodium diet (LS; n = 7) fed LDLR KO male mice was subjected to global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Statistical analysis was performed by using Student's t -test, with “false Discovery rate” (FDR) considering p ≤ 0.05. AC, acylcarnitine; CL, cardiolipins; FFA, free fatty acids; PC, phosphatidylcholine; PI, phosphatidylinositol; pPE, plasmenyl phosphattidylethanolamine.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, High Performance Liquid Chromatography

    A and B, Cardiopilin (CL) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, UHPLC-MS/MS coupled to ESI-Q-TOF-MS. CL species were separated in panels A and B according to their size, A ) 0.0 to 1.0 and B ) 0.0 to 6.0 μg/mg of protein. C , acylcarnitines (AC) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, LC-MS/MS coupled to ESI-Q-TOF-MS. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: A and B, Cardiopilin (CL) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, UHPLC-MS/MS coupled to ESI-Q-TOF-MS. CL species were separated in panels A and B according to their size, A ) 0.0 to 1.0 and B ) 0.0 to 6.0 μg/mg of protein. C , acylcarnitines (AC) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, LC-MS/MS coupled to ESI-Q-TOF-MS. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, Tandem Mass Spectroscopy, Liquid Chromatography with Mass Spectroscopy, MANN-WHITNEY

    Free fatty acids (FFA) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. FFA species were separated in panels A – C according to their size, A ) 0.0 to 0.3, B ) 0.0 to 0.02 and 0.0 to 1.0, C ) 0.0 to 8.0 μg/mg of protein. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: Free fatty acids (FFA) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. FFA species were separated in panels A – C according to their size, A ) 0.0 to 0.3, B ) 0.0 to 0.02 and 0.0 to 1.0, C ) 0.0 to 8.0 μg/mg of protein. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, High Performance Liquid Chromatography, MANN-WHITNEY

    Phosphatidylcholine (PC) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed a normal (NS; n = 7) or low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. PC species were separated in panels A – D according to their size, A ) 0.0 to 0.15, B ) 0.0 to 1.0, C ) 0.0 to 1.5 and D ) 0.0 to 15 μg/mg of protein. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: Phosphatidylcholine (PC) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed a normal (NS; n = 7) or low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. PC species were separated in panels A – D according to their size, A ) 0.0 to 0.15, B ) 0.0 to 1.0, C ) 0.0 to 1.5 and D ) 0.0 to 15 μg/mg of protein. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, High Performance Liquid Chromatography, MANN-WHITNEY

    Phosphatidylinositol (PI) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: Phosphatidylinositol (PI) species identified in the gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n = 7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry, ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Values expressed as median (interquartile range) were compared by the Mann–Whitney test.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, High Performance Liquid Chromatography, MANN-WHITNEY

    A , relative percentage of the six most abundant lipid categories identified in gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n =7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry,ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Values expressed as average ± standard deviation were compared by the Mann–Whitney test. B , PC:PE ratio. CL, cardiolipins; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; pPC, plasmenyl diacylphosphatidylcholine; pPE, plasmenyl phosphattidylethanolamine. Values expressed as median (interquartile range) were compared by the Mann–Whitney test. C , total lipid mass of DG, TG, Cer and SM in the gastrocnemius. Values expressed as median (interquartile range) were compared by the Mann–Whitney test. Cer, ceramides; DG, diacylglycerol; SM, sphingomyelin; TG, triacylglycerol.

    Journal: The Journal of Biological Chemistry

    Article Title: Dietary sodium restriction alters muscle lipidomics that relates to insulin resistance in mice

    doi: 10.1016/j.jbc.2021.100344

    Figure Lengend Snippet: A , relative percentage of the six most abundant lipid categories identified in gastrocnemius. The lipid extract of the gastrocnemius excised from LDLR KO male mice fed either a normal (NS; n = 7) or a low-sodium diet (LS; n =7) was subjected to a global lipidomic analysis performed with high-resolution mass spectrometry,ultra-high-performance liquid chromatography coupled to mass spectrometry coupled to ESI-Q-TOF-MS. Values expressed as average ± standard deviation were compared by the Mann–Whitney test. B , PC:PE ratio. CL, cardiolipins; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; pPC, plasmenyl diacylphosphatidylcholine; pPE, plasmenyl phosphattidylethanolamine. Values expressed as median (interquartile range) were compared by the Mann–Whitney test. C , total lipid mass of DG, TG, Cer and SM in the gastrocnemius. Values expressed as median (interquartile range) were compared by the Mann–Whitney test. Cer, ceramides; DG, diacylglycerol; SM, sphingomyelin; TG, triacylglycerol.

    Article Snippet: For global lipidomics analysis, total gastrocnemius lipid extract was analyzed by ultrahigh-performance liquid chromatography (UHPLC, Nexera, Shimadzu, Kyoto, Japan) coupled to ESI-Q-TOF-MS (TripleTOF 6600, Sciex, Concord, Estados Unidos) as previously described by Chaves-Filho et al. (2019) ( ).

    Techniques: Mouse Assay, Mass Spectrometry, High Performance Liquid Chromatography, Standard Deviation, MANN-WHITNEY