liquid chromatography electrospray ionization tandem mass spectrometry  (Millipore)


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    Name:
    alpha tert Butyl beta 4 chlorobenzyl 1H 1 2 4 triazole 1 ethanol
    Description:
    α tert Butyl β 4 chlorobenzyl 1H 1 2 4 triazole 1 ethanol is a known plant growth regulator which is utilized to inhibit the alternate bearing habit of mango Mangifera indica L
    Catalog Number:
    46046
    Price:
    None
    Applications:
    α-tert-Butyl-β-(4-chlorobenzyl)-1H-1,2,4-triazole-1-ethanol may be used as a reference standard for the determination of the analyte in:. Apple juice samples by matrix solid-phase dispersion (MSPD) and gas chromatography-mass selective detection (GC-MSD) with electron impact (EI) ionization.. Processed fruits and vegetables by miniaturized extraction-partition and liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) equipped with multiple reaction monitoring (MRM) detection.. Crops and soya grains by LC-ESI-MS/MS with MRM detection.
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    Structured Review

    Millipore liquid chromatography electrospray ionization tandem mass spectrometry
    alpha tert Butyl beta 4 chlorobenzyl 1H 1 2 4 triazole 1 ethanol
    α tert Butyl β 4 chlorobenzyl 1H 1 2 4 triazole 1 ethanol is a known plant growth regulator which is utilized to inhibit the alternate bearing habit of mango Mangifera indica L
    https://www.bioz.com/result/liquid chromatography electrospray ionization tandem mass spectrometry/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography electrospray ionization tandem mass spectrometry - by Bioz Stars, 2020-09
    99/100 stars

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    Article Title: ICE1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development
    Article Snippet: Sterile seeds were plated onto filter paper in Petri dishes containing 20 μm paclobutrazol (Sigma Aldrich, cat. no. 46046).

    Article Title: A molecular basis behind heterophylly in an amphibious plant, Ranunculus trichophyllus
    Article Snippet: For submerged treatment, abscisic acid (ABA, Sigma Aldrich, A0149), silver nitrate (AgNO3 , Sigma Aldrich, S8157), and paclobutrazol (PBZ, Sigma Aldrich, 46046) were added into the aquatic media.

    Article Title: Epithelial layer unjamming shifts energy metabolism toward glycolysis
    Article Snippet: Binning lifetime data in both space (i.e., adding photon counts from a moving window of 2o pixels on each side of the current pixel) and time (i.e., adding photon counts from 4 consecutive time bins to achieve a total of 64 time bins) was implemented to maximize the total photon count in the temporal decays used for further analysis where C represents a constant level of background, while τ 1 and τ 2 represent, respectively, the fast and slow rates of decay with fractional contributions α 1 and α 2 (with α 1 + α 2 = 1).

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  • 99
    Millipore tmz
    Therapeutic effect of <t>ALZ003</t> on the growth of <t>TMZ-resistant</t> glioblastoma in vivo . A. After intracranial transplantation with TMZ-resistant U87MG cells for 10 days, mice were orally and intravenously administrated with TMZ (15 mg/kg) and ALZ003 three times per week, respectively. The date of death was recorded and analyzed by Log-Rank Test. (** p = 0.0052). B. The mouse brains were paraffin embedded and subject to slides preparation followed by hematoxylin- and eosin-staining. Tumor in each slide was indicated. The scale bar for HE staining of whole brain slice is 1 mm, and that for other IHC images is 0.5 mm. C. The area of tumor was quantitated by the Image J software. The difference with the control group was analyzed using Student's t -test (* p
    Tmz, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmz/product/Millipore
    Average 99 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    tmz - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Millipore pkg inhibitor
    Schematic representation of <t>BNP</t> signaling pathway leading to Sca-1 + cell proliferation. ( A ) Number of sorted Sca-1 high+ cells after 11 days of culture with or without BNP or CNP and <t>PKG</t> or p38 inhibitor. n = at least 6 different experiments per group. The data were related to the average of untreated cells. All the results are means ± SEM, *p
    Pkg Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pkg inhibitor/product/Millipore
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pkg inhibitor - by Bioz Stars, 2020-09
    99/100 stars
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    Therapeutic effect of ALZ003 on the growth of TMZ-resistant glioblastoma in vivo . A. After intracranial transplantation with TMZ-resistant U87MG cells for 10 days, mice were orally and intravenously administrated with TMZ (15 mg/kg) and ALZ003 three times per week, respectively. The date of death was recorded and analyzed by Log-Rank Test. (** p = 0.0052). B. The mouse brains were paraffin embedded and subject to slides preparation followed by hematoxylin- and eosin-staining. Tumor in each slide was indicated. The scale bar for HE staining of whole brain slice is 1 mm, and that for other IHC images is 0.5 mm. C. The area of tumor was quantitated by the Image J software. The difference with the control group was analyzed using Student's t -test (* p

    Journal: Redox Biology

    Article Title: AR ubiquitination induced by the curcumin analog suppresses growth of temozolomide-resistant glioblastoma through disrupting GPX4-Mediated redox homeostasis

    doi: 10.1016/j.redox.2019.101413

    Figure Lengend Snippet: Therapeutic effect of ALZ003 on the growth of TMZ-resistant glioblastoma in vivo . A. After intracranial transplantation with TMZ-resistant U87MG cells for 10 days, mice were orally and intravenously administrated with TMZ (15 mg/kg) and ALZ003 three times per week, respectively. The date of death was recorded and analyzed by Log-Rank Test. (** p = 0.0052). B. The mouse brains were paraffin embedded and subject to slides preparation followed by hematoxylin- and eosin-staining. Tumor in each slide was indicated. The scale bar for HE staining of whole brain slice is 1 mm, and that for other IHC images is 0.5 mm. C. The area of tumor was quantitated by the Image J software. The difference with the control group was analyzed using Student's t -test (* p

    Article Snippet: Both of TMZ and ALZ003 were dissolved in dimethyl sulfoxide (DMSO, MilliporeSigma Corporate), and stored at −20 °C. siRNA targeting AR or GPX4 was purchased from GE Healthcare Dharmacon, Inc. (Lafayette, CO, USA).

    Techniques: In Vivo, Transplantation Assay, Mouse Assay, Staining, Slice Preparation, Immunohistochemistry, Software

    Potential death mode triggered by verapamil and comparison of the effect of verapamil with that of other drugs that synergize with adenovirus. ( a ) Apoptosis activation status in Ad5-infected A549 cells in the presence or absence of verapamil. PARP cleavage was detected by western blot at 40 hours postinfection. Cells incubated with Staurosporine (SSP, 0.2 µmol/l) are used as positive control of apoptosis induction. ( b ) Anti-LC3 western blot in A549 cells in the presence of calcium channel blockers. A549 cells were infected with Ad5 and incubated with normal medium or medium containing rapamycin (positive control of autophagy induction), verapamil (V), amlodipine (A), or diltiazem (D). ( c ) Virus release in the presence of rapamycin. A549 cells were incubated with increasing concentrations of rapamycin or verapamil, and LC3 expression was assessed by western blot 40 hours p.i. At the same conditions, the extracellular and total virus produced were quantified at 40 hours p.i. Mean values ( n = 3) ± SD are plotted. *Significant compared to the release of Ad5 ( P = 0.04). ( d ) Plaque size of Ad5 in the presence of 30 µmol/l verapamil (V) or 5 µmol/l cisplatin (CDDP), 200 pg/ml docetaxel (Doce), 10 nmol/l RAD001, or 10 µmol/l temozolomide (TMZ) (concentration that gave 10% growth inhibition). Cells were stained 7 days postinfection and pictures of representative plaques are shown. p.i., postinfection.

    Journal: Molecular Therapy

    Article Title: Verapamil Enhances the Antitumoral Efficacy of Oncolytic Adenoviruses

    doi: 10.1038/mt.2010.22

    Figure Lengend Snippet: Potential death mode triggered by verapamil and comparison of the effect of verapamil with that of other drugs that synergize with adenovirus. ( a ) Apoptosis activation status in Ad5-infected A549 cells in the presence or absence of verapamil. PARP cleavage was detected by western blot at 40 hours postinfection. Cells incubated with Staurosporine (SSP, 0.2 µmol/l) are used as positive control of apoptosis induction. ( b ) Anti-LC3 western blot in A549 cells in the presence of calcium channel blockers. A549 cells were infected with Ad5 and incubated with normal medium or medium containing rapamycin (positive control of autophagy induction), verapamil (V), amlodipine (A), or diltiazem (D). ( c ) Virus release in the presence of rapamycin. A549 cells were incubated with increasing concentrations of rapamycin or verapamil, and LC3 expression was assessed by western blot 40 hours p.i. At the same conditions, the extracellular and total virus produced were quantified at 40 hours p.i. Mean values ( n = 3) ± SD are plotted. *Significant compared to the release of Ad5 ( P = 0.04). ( d ) Plaque size of Ad5 in the presence of 30 µmol/l verapamil (V) or 5 µmol/l cisplatin (CDDP), 200 pg/ml docetaxel (Doce), 10 nmol/l RAD001, or 10 µmol/l temozolomide (TMZ) (concentration that gave 10% growth inhibition). Cells were stained 7 days postinfection and pictures of representative plaques are shown. p.i., postinfection.

    Article Snippet: RAD001 (everolimus) was supplied by Novartis (Basel, Switzerland), and rapamycin, cisplatin, docetaxel (Taxotere), and temozolomide (Temodal) were purchased from Calbiochem (Darmstadt, Germany), Sigma-Aldrich, Aventis Pharma (Dagenham, UK), and Schering-Plough (Madrid, Spain), respectively.

    Techniques: Activation Assay, Infection, Western Blot, Incubation, Positive Control, Expressing, Produced, Concentration Assay, Inhibition, Staining

    Schematic representation of BNP signaling pathway leading to Sca-1 + cell proliferation. ( A ) Number of sorted Sca-1 high+ cells after 11 days of culture with or without BNP or CNP and PKG or p38 inhibitor. n = at least 6 different experiments per group. The data were related to the average of untreated cells. All the results are means ± SEM, *p

    Journal: Scientific Reports

    Article Title: Natriuretic Peptide Receptor B modulates the proliferation of the cardiac cells expressing the Stem Cell Antigen-1

    doi: 10.1038/srep41936

    Figure Lengend Snippet: Schematic representation of BNP signaling pathway leading to Sca-1 + cell proliferation. ( A ) Number of sorted Sca-1 high+ cells after 11 days of culture with or without BNP or CNP and PKG or p38 inhibitor. n = at least 6 different experiments per group. The data were related to the average of untreated cells. All the results are means ± SEM, *p

    Article Snippet: To determine by which signaling pathway BNP acts on Sca-1high+ cells to induce their proliferation, a PKG inhibitor (Guanosine 3′, 5′-cyclic Monophosphorothioate, β-Phenyl-1, N2-etheno-8-bromo-Rp-Isomer, Sodium Salt (Calbiochem) 0.1 microM)) or a p38 MAP kinase inhibitor (SB203580 (Enzo) 10 microM) was added to the cell culture 15 min or 1 hour, respectively, before the BNP treatment.

    Techniques:

    RASIP1 is a RAP1 effector that controls EC junction morphology. (A) Pull-down assay using purified RAP1A, RHOA, and RAC1 GST fusion proteins with control (c) or RASIP1 shRNA (kd) HUVEC lysates. RASIP1 interacts weakly with RAP1A-GST loaded with GDP, and

    Journal: Blood

    Article Title: Rasip1 regulates vertebrate vascular endothelial junction stability through Epac1-Rap1 signaling

    doi: 10.1182/blood-2013-02-483156

    Figure Lengend Snippet: RASIP1 is a RAP1 effector that controls EC junction morphology. (A) Pull-down assay using purified RAP1A, RHOA, and RAC1 GST fusion proteins with control (c) or RASIP1 shRNA (kd) HUVEC lysates. RASIP1 interacts weakly with RAP1A-GST loaded with GDP, and

    Article Snippet: Purified RAP1A–glutathione S-transferase (GST) (Sigma-Aldrich), Ras homolog gene family member A (RHOA)-GST, and RAC1A-GST (Novus) were incubated in cell lysis buffer with 10 μM guanosine diphosphate (GDP) or GTPγS (Millipore) for 30 minutes at 37°C.

    Techniques: Pull Down Assay, Purification, shRNA