Structured Review

Difco liquid 7h9 medium
Sensitivity of the wild type and the Δ bfrB mutant to antibiotics. The wild-type (▲) and Δ bfrB (●) strains were cultured for 2 days in <t>7H9</t> medium with no drug added or with increasing concentrations of antibiotics. Dilutions
Liquid 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid 7h9 medium/product/Difco
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
liquid 7h9 medium - by Bioz Stars, 2020-01
92/100 stars

Images

1) Product Images from "A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice"

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice

Journal:

doi: 10.1128/IAI.00229-12

Sensitivity of the wild type and the Δ bfrB mutant to antibiotics. The wild-type (▲) and Δ bfrB (●) strains were cultured for 2 days in 7H9 medium with no drug added or with increasing concentrations of antibiotics. Dilutions
Figure Legend Snippet: Sensitivity of the wild type and the Δ bfrB mutant to antibiotics. The wild-type (▲) and Δ bfrB (●) strains were cultured for 2 days in 7H9 medium with no drug added or with increasing concentrations of antibiotics. Dilutions

Techniques Used: Mutagenesis, Cell Culture

Growth of M. tuberculosis strains in 7H9 medium. Wild-type strain H37Rv (◆), the Δ bfrA mutant (▲), the Δ bfrB mutant (●), and the complemented Δ bfrB mutant (■) were grown in 7H9 medium with agitation
Figure Legend Snippet: Growth of M. tuberculosis strains in 7H9 medium. Wild-type strain H37Rv (◆), the Δ bfrA mutant (▲), the Δ bfrB mutant (●), and the complemented Δ bfrB mutant (■) were grown in 7H9 medium with agitation

Techniques Used: Mutagenesis

2) Product Images from "An ethA-ethR-Deficient Mycobacterium bovis BCG Mutant Displays Increased Adherence to Mammalian Cells and Greater Persistence In Vivo, Which Correlate with Altered Mycolic Acid Composition"

Article Title: An ethA-ethR-Deficient Mycobacterium bovis BCG Mutant Displays Increased Adherence to Mammalian Cells and Greater Persistence In Vivo, Which Correlate with Altered Mycolic Acid Composition

Journal:

doi: 10.1128/IAI.01332-13

Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in
Figure Legend Snippet: Mass spectrometry analysis of MAs. MAs were extracted from mid-log-phase liquid 7H9 medium cultures. Samples were analyzed via a QTRAP 4000 mass spectrometer. (A) Individual sums of C 26 α-, C 24 α-, C 26 keto-, and C 24 keto-MA profiles in

Techniques Used: Mass Spectrometry

Related Articles

Clone Assay:

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice
Article Snippet: Escherichia coli strains JM109 and XL-10 (Stratagene) were used for cloning and were grown in Luria-Bertani (LB) broth. .. M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ).

Centrifugation:

Article Title: Quantitative Proteomic Profiling of Host-Pathogen Interactions: The Macrophage Response to Mycobacterium tuberculosis Lipids
Article Snippet: M. tb strain H37Rv was grown to stationary phase in liquid 7H9 medium (Difco) supplemented with 10% OADC, 0.5% glycerol, and 0.1% Tween80. .. Ten (10) mL of culture was pelleted by centrifugation (15,000 × g), washed with PBS and extracted with 2.5 mL of 2:1 chloroform:methanol by vigorous shaking for 2 h at rt.

Filtration:

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice
Article Snippet: M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ). .. M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ).

Electron Microscopy:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex. .. PCR genotyping of the Mtb RD9 region was performed by amplifying genomic DNA from putative Mtb CFU using the primer set RD9/qRTF ( 5’-tgagtggcgatggtcaacac-3’ ) and RD9/qRTR ( 5’-gatggcgttcggaaagaaac-3’ ).

Mutagenesis:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: The M . smegmatis ::gfp reporter mutant expressing green fluorescent protein (GFP) was constructed by introduction of the pMSP12GFP plasmid [ ] into M . smegmatis mc2 155. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Isolation:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: The M . smegmatis ::gfp reporter mutant expressing green fluorescent protein (GFP) was constructed by introduction of the pMSP12GFP plasmid [ ] into M . smegmatis mc2 155. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex. .. Owing to the selectivity provided by the aph cassette on pMSP12GFP, all media contained kanamycin (Sigma) at a final concentration of 20 μg/mL.

Atomic Absorption Spectroscopy:

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice
Article Snippet: M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ). .. Chelex was removed by filtration, and before use, the medium was supplemented with 0.5 mg ZnCl2 /liter, 0.1 mg/liter MnSO4 , and 40 mg/liter MgSO4 .

Microscopy:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex. .. PCR genotyping of the Mtb RD9 region was performed by amplifying genomic DNA from putative Mtb CFU using the primer set RD9/qRTF ( 5’-tgagtggcgatggtcaacac-3’ ) and RD9/qRTR ( 5’-gatggcgttcggaaagaaac-3’ ).

Cell Culture:

Article Title: Quantitative Proteomic Profiling of Host-Pathogen Interactions: The Macrophage Response to Mycobacterium tuberculosis Lipids
Article Snippet: M. tb strain H37Rv was grown to stationary phase in liquid 7H9 medium (Difco) supplemented with 10% OADC, 0.5% glycerol, and 0.1% Tween80. .. Ten (10) mL of culture was pelleted by centrifugation (15,000 × g), washed with PBS and extracted with 2.5 mL of 2:1 chloroform:methanol by vigorous shaking for 2 h at rt.

Construct:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: The M . smegmatis ::gfp reporter mutant expressing green fluorescent protein (GFP) was constructed by introduction of the pMSP12GFP plasmid [ ] into M . smegmatis mc2 155. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Expressing:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: The M . smegmatis ::gfp reporter mutant expressing green fluorescent protein (GFP) was constructed by introduction of the pMSP12GFP plasmid [ ] into M . smegmatis mc2 155. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Polymerase Chain Reaction:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Sonication:

Article Title: Quantitative Proteomic Profiling of Host-Pathogen Interactions: The Macrophage Response to Mycobacterium tuberculosis Lipids
Article Snippet: M. tb strain H37Rv was grown to stationary phase in liquid 7H9 medium (Difco) supplemented with 10% OADC, 0.5% glycerol, and 0.1% Tween80. .. Ten (10) mL of culture was pelleted by centrifugation (15,000 × g), washed with PBS and extracted with 2.5 mL of 2:1 chloroform:methanol by vigorous shaking for 2 h at rt.

Plasmid Preparation:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: The M . smegmatis ::gfp reporter mutant expressing green fluorescent protein (GFP) was constructed by introduction of the pMSP12GFP plasmid [ ] into M . smegmatis mc2 155. .. For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Imaging:

Article Title: Real-Time Investigation of Tuberculosis Transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC)
Article Snippet: Paragraph title: Bacteriological, Molecular and Imaging Analyses ... For determination of CFU in controlled aerosol release experiments, bacilli were propagated in liquid 7H9 medium (Difco) supplemented with 0.05% Tween-80, 0.2% glycerol and albumin/NaCl/ glucose (ADC) complex and/or isolated on solid 7H10 agar (Difco) supplemented with 0.2% glycerol and oleic acid/albumin/dextrose/catalase (OADC) complex.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 87
    Difco 7h9 liquid culture medium
    Malachite green interferes with postantibiotic recovery of M. smegmatis on solid culture medium. (A and B) M. smegmatis was grown to mid-exponential phase in Middlebrook <t>7H9</t> broth and incubation was continued after the addition of 50 μg/ml isoniazid
    7h9 Liquid Culture Medium, supplied by Difco, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid culture medium/product/Difco
    Average 87 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid culture medium - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

    Image Search Results


    Malachite green interferes with postantibiotic recovery of M. smegmatis on solid culture medium. (A and B) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth and incubation was continued after the addition of 50 μg/ml isoniazid

    Journal:

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Malachite green interferes with postantibiotic recovery of M. smegmatis on solid culture medium. (A and B) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth and incubation was continued after the addition of 50 μg/ml isoniazid

    Article Snippet: For experiments with catalase, bacteria were grown with aeration at 37°C in 7H9 liquid culture medium containing 0.5% glycerol and 10% OADC (Difco) as a source of catalase.

    Techniques: Incubation

    Inhibition of postantibiotic recovery by malachite green is specific to antibiotics that target cell wall biogenesis. (A to F) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 24 h after

    Journal:

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Inhibition of postantibiotic recovery by malachite green is specific to antibiotics that target cell wall biogenesis. (A to F) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 24 h after

    Article Snippet: For experiments with catalase, bacteria were grown with aeration at 37°C in 7H9 liquid culture medium containing 0.5% glycerol and 10% OADC (Difco) as a source of catalase.

    Techniques: Inhibition, Incubation

    Effect of albumin or ROS scavengers on inhibition of postantibiotic recovery by malachite green. (A to C) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth (A and C) or in 7H9 broth containing 10% OADC (B) and incubation was continued

    Journal:

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Effect of albumin or ROS scavengers on inhibition of postantibiotic recovery by malachite green. (A to C) M. smegmatis was grown to mid-exponential phase in Middlebrook 7H9 broth (A and C) or in 7H9 broth containing 10% OADC (B) and incubation was continued

    Article Snippet: For experiments with catalase, bacteria were grown with aeration at 37°C in 7H9 liquid culture medium containing 0.5% glycerol and 10% OADC (Difco) as a source of catalase.

    Techniques: Inhibition, Incubation

    Malachite green interferes with postantibiotic recovery of M. tuberculosis on solid culture medium. (A to F) M. tuberculosis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 48 h after the addition

    Journal:

    Article Title: Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

    doi: 10.1128/AAC.00406-12

    Figure Lengend Snippet: Malachite green interferes with postantibiotic recovery of M. tuberculosis on solid culture medium. (A to F) M. tuberculosis was grown to mid-exponential phase in Middlebrook 7H9 broth, and incubation was continued for another 48 h after the addition

    Article Snippet: For experiments with catalase, bacteria were grown with aeration at 37°C in 7H9 liquid culture medium containing 0.5% glycerol and 10% OADC (Difco) as a source of catalase.

    Techniques: Incubation

    Construction of MSMEG_1764 knockout mutant and complement strains. ( A ) PCR verification of the construction MSMEG_1764 knockout strain. Lanes: 1.Wild-type MS; 2. MSMEG_1764 knockout strain. ( B ) Verify the transcription of MSMEG_1764 knockout strain by RT-PCR. Wild type and Δ lat Msm strains were grown at 37C in MB 7H9 liquid medium to an OD600 of 0.8–1.0. Total bacterial RNA was isolated and subjected to RT-PCR to detect the expression of the lat Msm gene. ( C ) Construction of Δ lat-Rv3290c strain; Lanes: 1. Knockout strain complement with pALACE- Rv3290 c; 2. Knockout strain complement with pALACE plasmid. ( D ) Western-blotting to confirm the expression of Rv3290c.Lysates were prepared from bacterial cells cultured as in ( B ), after 16 h induction and subjected to Western blotting to detect His-tagged Rv3290c protein using mouse anti-His antibody.

    Journal: Scientific Reports

    Article Title: Mycobacterium Lysine ε-aminotransferase is a novel alarmone metabolism related persister gene via dysregulating the intracellular amino acid level

    doi: 10.1038/srep19695

    Figure Lengend Snippet: Construction of MSMEG_1764 knockout mutant and complement strains. ( A ) PCR verification of the construction MSMEG_1764 knockout strain. Lanes: 1.Wild-type MS; 2. MSMEG_1764 knockout strain. ( B ) Verify the transcription of MSMEG_1764 knockout strain by RT-PCR. Wild type and Δ lat Msm strains were grown at 37C in MB 7H9 liquid medium to an OD600 of 0.8–1.0. Total bacterial RNA was isolated and subjected to RT-PCR to detect the expression of the lat Msm gene. ( C ) Construction of Δ lat-Rv3290c strain; Lanes: 1. Knockout strain complement with pALACE- Rv3290 c; 2. Knockout strain complement with pALACE plasmid. ( D ) Western-blotting to confirm the expression of Rv3290c.Lysates were prepared from bacterial cells cultured as in ( B ), after 16 h induction and subjected to Western blotting to detect His-tagged Rv3290c protein using mouse anti-His antibody.

    Article Snippet: E.coli strains were grown on LB broth agar or in LB broth, Mycobacterium smegmatis mc2 155 was grown in 7H9 liquid medium (Difco) supplemented with 0.05% w/v Tween 80, 0.5%glycerol and 0.5%glucose or were grown on 7H10 agar supplemented with 1% glycerol and 0.5% glucose.

    Techniques: Knock-Out, Mutagenesis, Polymerase Chain Reaction, Mass Spectrometry, Reverse Transcription Polymerase Chain Reaction, Isolation, Expressing, Plasmid Preparation, Western Blot, Cell Culture

    The expression of M. smegmatis lat under nutrient starvation. Cells of M. smegmatis were initially grown in 7H9 medium to log-phase and then washed by 1 × PBS and resuspended in 1 × PBS, incubate at 37 °C, 110 rpm. RNAs were extracted from bacteria harvested at indicated time points. RT-PCR was performed as described in Materials and Methods. Data are means ± s.d. of triplicates in one of at least three experiments.

    Journal: Scientific Reports

    Article Title: Mycobacterium Lysine ε-aminotransferase is a novel alarmone metabolism related persister gene via dysregulating the intracellular amino acid level

    doi: 10.1038/srep19695

    Figure Lengend Snippet: The expression of M. smegmatis lat under nutrient starvation. Cells of M. smegmatis were initially grown in 7H9 medium to log-phase and then washed by 1 × PBS and resuspended in 1 × PBS, incubate at 37 °C, 110 rpm. RNAs were extracted from bacteria harvested at indicated time points. RT-PCR was performed as described in Materials and Methods. Data are means ± s.d. of triplicates in one of at least three experiments.

    Article Snippet: E.coli strains were grown on LB broth agar or in LB broth, Mycobacterium smegmatis mc2 155 was grown in 7H9 liquid medium (Difco) supplemented with 0.05% w/v Tween 80, 0.5%glycerol and 0.5%glucose or were grown on 7H10 agar supplemented with 1% glycerol and 0.5% glucose.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Sensitivity of the wild type and the Δ bfrB mutant to antibiotics. The wild-type (▲) and Δ bfrB (●) strains were cultured for 2 days in 7H9 medium with no drug added or with increasing concentrations of antibiotics. Dilutions

    Journal:

    Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice

    doi: 10.1128/IAI.00229-12

    Figure Lengend Snippet: Sensitivity of the wild type and the Δ bfrB mutant to antibiotics. The wild-type (▲) and Δ bfrB (●) strains were cultured for 2 days in 7H9 medium with no drug added or with increasing concentrations of antibiotics. Dilutions

    Article Snippet: M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ).

    Techniques: Mutagenesis, Cell Culture

    Growth of M. tuberculosis strains in 7H9 medium. Wild-type strain H37Rv (◆), the Δ bfrA mutant (▲), the Δ bfrB mutant (●), and the complemented Δ bfrB mutant (■) were grown in 7H9 medium with agitation

    Journal:

    Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice

    doi: 10.1128/IAI.00229-12

    Figure Lengend Snippet: Growth of M. tuberculosis strains in 7H9 medium. Wild-type strain H37Rv (◆), the Δ bfrA mutant (▲), the Δ bfrB mutant (●), and the complemented Δ bfrB mutant (■) were grown in 7H9 medium with agitation

    Article Snippet: M. tuberculosis cells were grown in liquid 7H9 medium (Difco) or in low-iron defined medium (LIMM) prepared as previously described ( ).

    Techniques: Mutagenesis

    Growth of H37Rv, Erdman, rv3671c -TetON, prcBA -TetON, and icl -TetON in multi-strain liquid cultures. Multi-strain cultures were prepared, grown in 7H9 medium ( A ), Sauton's medium containing butyrate ( B ) or glycerol ( C ) and analyzed as indicated in Figure 2 . Black and clear symbols identify data generated from atc-containing or atc-free cultures, respectively. Data are averages of triplicate cultures and representative of at least two independent experiments. Error bars (representing standard deviations) cannot be seen because they are smaller than the data point symbols.

    Journal: PLoS ONE

    Article Title: Simultaneous Analysis of Multiple Mycobacterium tuberculosis Knockdown Mutants In Vitro and In Vivo

    doi: 10.1371/journal.pone.0015667

    Figure Lengend Snippet: Growth of H37Rv, Erdman, rv3671c -TetON, prcBA -TetON, and icl -TetON in multi-strain liquid cultures. Multi-strain cultures were prepared, grown in 7H9 medium ( A ), Sauton's medium containing butyrate ( B ) or glycerol ( C ) and analyzed as indicated in Figure 2 . Black and clear symbols identify data generated from atc-containing or atc-free cultures, respectively. Data are averages of triplicate cultures and representative of at least two independent experiments. Error bars (representing standard deviations) cannot be seen because they are smaller than the data point symbols.

    Article Snippet: Unless otherwise noted, mycobacteria were grown in Middlebrook 7H9 liquid medium (Difco) containing 0.2% glycerol, 0.05% Tween80, 0.5% BSA, 0.2% dextrose, and 0.085% sodium chloride.

    Techniques: Generated

    Impact of silencing rv3671c or prcBA on growth of Mtb in different liquid media. Growth of Mtb H37Rv (squares), rv3671c -TetON (triangles), prcBA -TetON (diamonds) without atc was analyzed in 7H9 medium ( A,D ), Sauton's medium containing butyrate ( B,E ) or glycerol ( C,F ) as the primary carbon source using optical density measurements at the indicated time points. Data represent results of three ( rv3671c -TetON) and two ( prcBA -TetON) independent experiments.

    Journal: PLoS ONE

    Article Title: Simultaneous Analysis of Multiple Mycobacterium tuberculosis Knockdown Mutants In Vitro and In Vivo

    doi: 10.1371/journal.pone.0015667

    Figure Lengend Snippet: Impact of silencing rv3671c or prcBA on growth of Mtb in different liquid media. Growth of Mtb H37Rv (squares), rv3671c -TetON (triangles), prcBA -TetON (diamonds) without atc was analyzed in 7H9 medium ( A,D ), Sauton's medium containing butyrate ( B,E ) or glycerol ( C,F ) as the primary carbon source using optical density measurements at the indicated time points. Data represent results of three ( rv3671c -TetON) and two ( prcBA -TetON) independent experiments.

    Article Snippet: Unless otherwise noted, mycobacteria were grown in Middlebrook 7H9 liquid medium (Difco) containing 0.2% glycerol, 0.05% Tween80, 0.5% BSA, 0.2% dextrose, and 0.085% sodium chloride.

    Techniques: