lipid transfection  (Bio-Rad)

 
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    Name:
    TransFectin Lipid Reagent
    Description:
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    Catalog Number:
    1703350
    Price:
    None
    Category:
    Lipid Transfection
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    Structured Review

    Bio-Rad lipid transfection
    TransFectin Lipid Reagent
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    https://www.bioz.com/result/lipid transfection/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid transfection - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
    Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
    Article Snippet: .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

    Article Title: Phospholipase PLA2G7, associated with aggressive prostate cancer, promotes prostate cancer cell migration and invasion and is inhibited by statins
    Article Snippet: .. The siRNAs were pipeted onto plates, followed by addition of the transfection agent (siLentFect Lipid Reagent, Bio-Rad Laboratories, Hercules, CA) and appropriate amount of cells. .. The PLA2G7 siRNAs were used either separately or as a pooled siRNA and the final siRNA concentration was 13 nM.

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Luciferase:

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Mutagenesis:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Construct:

    Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
    Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Transient Transfection Assay:

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Plasmid Preparation:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
    Article Snippet: .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

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  • 99
    Bio-Rad transfection reagent
    <t>Transfection</t> studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A
    Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection reagent/product/Bio-Rad
    Average 99 stars, based on 186 article reviews
    Price from $9.99 to $1999.99
    transfection reagent - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A

    Journal: BMC Medical Genetics

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

    doi: 10.1186/1471-2350-8-65

    Figure Lengend Snippet: Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A", B" and C") are shown. In HL-1 cells immunostaining with monoclonal desmoglein antibody DG 3.10 showed both the presence of well-assembled desmosomes (panel D', E' and F') and the reduced co-localisation between endogenous dsg and mutated DSC2 (yellow dots in panel E", F").

    Article Snippet: Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad).

    Techniques: Transfection, Cell Culture, Immunostaining, Staining

    Transfection efficiency comparison of various transfection reagents (PEI-Max, Transfectin, and Lipofectamine 3000). 293FT cells were transfected with various DNA transfection reagent complexes described as condition numbers. The expression levels of CYP3A4 were determined at 48 h post-transfection by CO-difference spectroscopy ( A ) and western blotting, in which the PVDF membrane was cut across into two sections individually containing the protein of interest, CYP3A4 (57 kDa) and calnexin (90 kDa) for improved visualisation ( B ). N.D. not determined.

    Journal: Scientific Reports

    Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells

    doi: 10.1038/s41598-020-71035-5

    Figure Lengend Snippet: Transfection efficiency comparison of various transfection reagents (PEI-Max, Transfectin, and Lipofectamine 3000). 293FT cells were transfected with various DNA transfection reagent complexes described as condition numbers. The expression levels of CYP3A4 were determined at 48 h post-transfection by CO-difference spectroscopy ( A ) and western blotting, in which the PVDF membrane was cut across into two sections individually containing the protein of interest, CYP3A4 (57 kDa) and calnexin (90 kDa) for improved visualisation ( B ). N.D. not determined.

    Article Snippet: Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Transfection, Expressing, Spectroscopy, Western Blot

    DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Reporter Gene Assay, Luciferase, Expressing, Construct

    DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Fluorescence, Microscopy, Expressing