lipid transfection  (Bio-Rad)

 
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    Name:
    TransFectin Lipid Reagent
    Description:
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    Catalog Number:
    1703350
    Price:
    None
    Category:
    Lipid Transfection
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    Structured Review

    Bio-Rad lipid transfection
    TransFectin Lipid Reagent
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    https://www.bioz.com/result/lipid transfection/product/Bio-Rad
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid transfection - by Bioz Stars, 2020-07
    97/100 stars

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    Related Articles

    Transfection:

    Article Title: Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells
    Article Snippet: .. HepG2 cells were transfected using the TransFectin reagent (Bio-Rad, Munich, Germany) with 1 μ g of HA-FoxO3a-WT-ER plasmid ( ). .. Activation of the accumulated FoxO3a protein was induced by treatment with the ER ligand 4-OHT 1 h before melatonin treatment.

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: .. Cells were transfected using TransFectin Lipid Reagent (Bio-Rad) and incubated for 48 h before CCCP treatment. .. The final concentration of siRNA was 30 nM.

    Article Title: USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling
    Article Snippet: .. Alkaline phosphatase assay C2C12 cells were transfected with siRNAs against mouse USP15 or mouse FoxO4 (300 pM each) using transfectin reagent and grown in DMEM with 5% FBS. .. Forty-eight hours post-transfection 100 ng ml−1 BMP2 was added for 2–4 days, and cells were lysed using CelLytic reagent (Sigma).

    Article Title: Knockdown of estrogen receptor β increases proliferation and affects the transcriptome of endometrial adenocarcinoma cells
    Article Snippet: .. After 24 h, cells were transfected with 60 nM siRNA in OptiMEM reduced serum medium using 8 μl of Transfectin reagent (BioRad, Hercules, USA). .. For knockdown of ERβ expression, we used an equimolar mixture of three different pre-designed Silencer siRNAs (20 nM each) (IDs: 145909, 145910 and 145911), Thermo Fisher, Waltham, USA), targeting different regions of ESR2 gene.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

    Mutagenesis:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    ALP Assay:

    Article Title: USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling
    Article Snippet: .. Alkaline phosphatase assay C2C12 cells were transfected with siRNAs against mouse USP15 or mouse FoxO4 (300 pM each) using transfectin reagent and grown in DMEM with 5% FBS. .. Forty-eight hours post-transfection 100 ng ml−1 BMP2 was added for 2–4 days, and cells were lysed using CelLytic reagent (Sigma).

    Incubation:

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: .. Cells were transfected using TransFectin Lipid Reagent (Bio-Rad) and incubated for 48 h before CCCP treatment. .. The final concentration of siRNA was 30 nM.

    Transient Transfection Assay:

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Plasmid Preparation:

    Article Title: Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells
    Article Snippet: .. HepG2 cells were transfected using the TransFectin reagent (Bio-Rad, Munich, Germany) with 1 μ g of HA-FoxO3a-WT-ER plasmid ( ). .. Activation of the accumulated FoxO3a protein was induced by treatment with the ER ligand 4-OHT 1 h before melatonin treatment.

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

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  • 97
    Bio-Rad transfection reagent
    <t>Transfection</t> studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A
    Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection reagent/product/Bio-Rad
    Average 97 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    transfection reagent - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A

    Journal: BMC Medical Genetics

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

    doi: 10.1186/1471-2350-8-65

    Figure Lengend Snippet: Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A", B" and C") are shown. In HL-1 cells immunostaining with monoclonal desmoglein antibody DG 3.10 showed both the presence of well-assembled desmosomes (panel D', E' and F') and the reduced co-localisation between endogenous dsg and mutated DSC2 (yellow dots in panel E", F").

    Article Snippet: Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad).

    Techniques: Transfection, Cell Culture, Immunostaining, Staining

    DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Reporter Gene Assay, Luciferase, Expressing, Construct

    DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Fluorescence, Microscopy, Expressing

    Chondrogenic differentiation of ad-MSCs in the presence of fd-ECM requires activation of Notch1 and β-catenin signaling. Ad-MSCs were cultured on plastic dishes (−) or on the fd-ECM (+) for the indicated number of days ( A , B ) Ad-MSCs cultured on plastic control dishes and on fd-ECM were evaluated for β-catenin expression using immunofluorescence assay. Ad-MSCs, cultured for two days and four days, were incubated with antibodies against β-catenin and DAPI was used to stain the DNA in the nucleus. Scale bar: 100 µm; ( C ) Ad-MSCs were transfected with Notch1 siRNA and the dominant negative Notch1 construct using Transfectin Lipid reagent. Evaluation of Notch1, Sox9 and β-catenin protein levels was done; and ( D ) ad-MSCs were transfected with β-catenin siRNA as described above and Notch1, Sox9, and β-catenin protein level was determined.

    Journal: International Journal of Molecular Sciences

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro

    doi: 10.3390/ijms17081259

    Figure Lengend Snippet: Chondrogenic differentiation of ad-MSCs in the presence of fd-ECM requires activation of Notch1 and β-catenin signaling. Ad-MSCs were cultured on plastic dishes (−) or on the fd-ECM (+) for the indicated number of days ( A , B ) Ad-MSCs cultured on plastic control dishes and on fd-ECM were evaluated for β-catenin expression using immunofluorescence assay. Ad-MSCs, cultured for two days and four days, were incubated with antibodies against β-catenin and DAPI was used to stain the DNA in the nucleus. Scale bar: 100 µm; ( C ) Ad-MSCs were transfected with Notch1 siRNA and the dominant negative Notch1 construct using Transfectin Lipid reagent. Evaluation of Notch1, Sox9 and β-catenin protein levels was done; and ( D ) ad-MSCs were transfected with β-catenin siRNA as described above and Notch1, Sox9, and β-catenin protein level was determined.

    Article Snippet: Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays.

    Techniques: Activation Assay, Cell Culture, Expressing, Immunofluorescence, Incubation, Staining, Transfection, Dominant Negative Mutation, Construct