lipid transfection reagent transfast  (Promega)

 
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    Name:
    TransFast Transfection Reagent
    Description:
    Cationic lipid based high efficiency transfection of eukaryotic cells
    Catalog Number:
    e2431
    Price:
    None
    Category:
    Cell Biology Reporter Assays Transfection Transfection Reagents
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    Structured Review

    Promega lipid transfection reagent transfast
    Cationic lipid based high efficiency transfection of eukaryotic cells
    https://www.bioz.com/result/lipid transfection reagent transfast/product/Promega
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid transfection reagent transfast - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Modulation of both activator protein-1 and nuclear factor-kappa B signal transduction of human T cells by amiodarone
    Article Snippet: .. We used the transfection reagent TransFast™ (Promega) to transfect plasmids into the cells instead of using electroporation. .. Briefly, on the day of the transfection, Jurkat T cells were adjusted to 1 × 106 mL−1 in serum-free media and evenly mixed with 2 mg of reporter plasmid (Stratagene, La Jolla, CA) and TransFast™ transfectant (6 mL) in triplicate.

    Transfection:

    Article Title: Modulation of both activator protein-1 and nuclear factor-kappa B signal transduction of human T cells by amiodarone
    Article Snippet: .. We used the transfection reagent TransFast™ (Promega) to transfect plasmids into the cells instead of using electroporation. .. Briefly, on the day of the transfection, Jurkat T cells were adjusted to 1 × 106 mL−1 in serum-free media and evenly mixed with 2 mg of reporter plasmid (Stratagene, La Jolla, CA) and TransFast™ transfectant (6 mL) in triplicate.

    Article Title: Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts
    Article Snippet: .. Various combinations of plasmid DNA (1 μg total) and 3 μL of transfection reagent (Transfast from Promega Corporation) were added to 1 mL of medium containing 1% FCS and mixed vigorously. .. The transfection mix contained Renilla luciferase and firefly luciferase plasmids at a ratio of 1 to 10.

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. The transfection efficiency of 1 was comparable to the commercial transfection reagent TransFast (Promega), a cationic lipid-based gene delivery vector. ..

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium
    Article Snippet: .. CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS reagent powder) and TransFast Transfection Reagent were purchased from Promega Corporation (Madison, WI, U.S.A.). .. Phenazine methosulfate (PMS, 95%) was purchased from MP Biomedicals (Santa Ana, CA, U.S.A.). eGFP lentiviral particles (LVP-340, 1 × 107 IFU × mL–1 , a puromycin gene under Rsv promoter allows the selection of transduced fluorescent positive cells) were purchased from GenTarget Inc. (San Diego, CA, U.S.A.).

    Article Title: Epidermal Growth Factor Triggers an Original, Caspase-independent Pituitary Cell Death with Heterogeneous Phenotype
    Article Snippet: .. The transient GH4C1 cell transfection was performed by using TransFast reagent (Promega, Madison, WI). ..

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. O-ethyl-dioleylphosphatidylcholinium-uridine ( O-Et-DOUPC ) was tested for transfection efficiency using a β-gal reporter assay and was shown to be as effective as the commercial transfection reagent TransFast, and more efficient than DOTAP ( ). ..

    Article Title: Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells ▿
    Article Snippet: .. HTLV-1-transformed cells were transfected with 1 μg of reporter constructs HTLV-1LTR-Luc, PG13-Luc, and NF-κB-Luc (kindly provided by Warner Green) using Transfast transfection reagent (Promega) according to the manufacturer's protocol. .. All transfections included 0.5 μg Rous sarcoma virus (RSV) β-galactosidase (β-Gal) plasmid as a control for transfection efficiency.

    Amplification:

    Article Title: Patterned transgene expression in multiple channel bridges after spinal cord injury
    Article Snippet: .. Plasmids were amplified in Escherichia coli DH5α, purified with Qiagen (Santa Clara, CA) reagents, and complexed with Transfast (Promega, Madison, WI) in a w/v ratio of 1:1.5. .. After incubation for 15 minutes, 10 µL of fibronectin (2 µg/µL) (Sigma-Aldrich, St. Louis, MO) was added to the complexes and pipetted into the channels of the disks that were deposited in 6 well plates.

    Reporter Assay:

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. O-ethyl-dioleylphosphatidylcholinium-uridine ( O-Et-DOUPC ) was tested for transfection efficiency using a β-gal reporter assay and was shown to be as effective as the commercial transfection reagent TransFast, and more efficient than DOTAP ( ). ..

    Construct:

    Article Title: Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells ▿
    Article Snippet: .. HTLV-1-transformed cells were transfected with 1 μg of reporter constructs HTLV-1LTR-Luc, PG13-Luc, and NF-κB-Luc (kindly provided by Warner Green) using Transfast transfection reagent (Promega) according to the manufacturer's protocol. .. All transfections included 0.5 μg Rous sarcoma virus (RSV) β-galactosidase (β-Gal) plasmid as a control for transfection efficiency.

    Purification:

    Article Title: Patterned transgene expression in multiple channel bridges after spinal cord injury
    Article Snippet: .. Plasmids were amplified in Escherichia coli DH5α, purified with Qiagen (Santa Clara, CA) reagents, and complexed with Transfast (Promega, Madison, WI) in a w/v ratio of 1:1.5. .. After incubation for 15 minutes, 10 µL of fibronectin (2 µg/µL) (Sigma-Aldrich, St. Louis, MO) was added to the complexes and pipetted into the channels of the disks that were deposited in 6 well plates.

    Proliferation Assay:

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium
    Article Snippet: .. CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS reagent powder) and TransFast Transfection Reagent were purchased from Promega Corporation (Madison, WI, U.S.A.). .. Phenazine methosulfate (PMS, 95%) was purchased from MP Biomedicals (Santa Ana, CA, U.S.A.). eGFP lentiviral particles (LVP-340, 1 × 107 IFU × mL–1 , a puromycin gene under Rsv promoter allows the selection of transduced fluorescent positive cells) were purchased from GenTarget Inc. (San Diego, CA, U.S.A.).

    Plasmid Preparation:

    Article Title: Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts
    Article Snippet: .. Various combinations of plasmid DNA (1 μg total) and 3 μL of transfection reagent (Transfast from Promega Corporation) were added to 1 mL of medium containing 1% FCS and mixed vigorously. .. The transfection mix contained Renilla luciferase and firefly luciferase plasmids at a ratio of 1 to 10.

    Article Title: Charge-reversal Lipids, Peptide-based Lipids, and Nucleoside-based Lipids for Gene Delivery
    Article Snippet: .. The transfection efficiency of 1 was comparable to the commercial transfection reagent TransFast (Promega), a cationic lipid-based gene delivery vector. ..

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  • 93
    Promega cationic lipid based transfection reagent
    In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro <t>transfection</t> efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
    Cationic Lipid Based Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid based transfection reagent/product/Promega
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cationic lipid based transfection reagent - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    99
    Promega lipid based transfection reagent fugene 6 hd
    In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro <t>transfection</t> efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p
    Lipid Based Transfection Reagent Fugene 6 Hd, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent fugene 6 hd/product/Promega
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent fugene 6 hd - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: In vitro gene silencing efficiency of various concentrations of siRNA (0.1, 0.2, 0.4 and 0.8 ng/µl) with/without mTat/PEI, INT, and mTat/PEI/INT in HSC-3 cells. In vitro transfection efficiency of siRNA with/without mTat/PEI, INT (0.015, 0.03 and 0.06% v/v), and mTat/PEI/INT (0.015, 0.03, and 0.06% v/v) in HSC-3 cells ( a ). β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( b ). Transfection efficiency of siRNA with mTat/PEI/INT at the different concentration. siRNA concentration was 0.8 ng/µl ( c ). β-actin protein was measured in the cell lysate of HSC-3 cells transfected with siRNA with/without mTat/PEI, INT, and mTat/PEI/INT incubated for 48 h ( d ). siRNA concentration was 0.8 ng/µl and INT concentration was 0.06% v/v. Significant difference between INT/siRNA and mTat/PEI/INT/siRNA, * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: In Vitro, Transfection, Quantitative RT-PCR, Expressing, Concentration Assay, Incubation

    Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Zeta potentials ( a ) and particle sizes (diameter) ( b ) of siRNA, siRNA with Tat/PEI, INT, and siRNA with mTat/PEI/INT. Effects of temperature on siRNA transfection efficiency with mTat/PEI, INT, or mTat/PEI/INT in HSC-3 cells at 4 °C (white bars) or 37 °C (black bars) ( c ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection

    Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Journal: Journal of Nanobiotechnology

    Article Title: Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway

    doi: 10.1186/s12951-019-0444-8

    Figure Lengend Snippet: Comparative transfection efficiency of FuGENE HD (FuGENE), X-tremeGENE (X-treme), SuperFect (Super), Lipofectamine 2000 (L-2000), Lipofectamine RNAiMAX (L-iMAX), INT, and mTat/PEI/INT using siRNA targeting β - actin in HSC-3 cells. β - actin mRNA was measured by QRT-PCR and then % remaining β - actin mRNA expression was calculated based on control as 100% ( a ). Cell viability of each of the reagent groups in HaCaT cells were evaluated by MTT assay under the same siRNA and transfection reagent volume ( b ). * p

    Article Snippet: Non-viral vectors To compare transfection efficiency of siRNA targeting β -actin (Santa Cruz Biotechnology, Inc. Dallas, TX) with several commercial reagents, Lipofectamine 2000 (Invitrogen, Carlsbad, CA), which is a cationic lipid-based transfection reagent, FuGENE HD (Promega Corp., Fitchburg, WI), which is a blend of lipids and other components, SuperFect (Qiagen, Valencia, CA), which is an activated dendrimer-based reagent, Lipofectamine RNAiMAX (Invitrogen), which is a cationic lipid-based transfection reagent, X-tremeGENE (Roche, Switzerland), which is a blend of lipids and other components, and INT (Polyplus-transfection, France) were used.

    Techniques: Transfection, Quantitative RT-PCR, Expressing, MTT Assay