sf9 cell line  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Roche sf9 cell line
    Expression profile of Se-AQP . ( A ) Western blot analysis. Se-AQP was transfected into <t>Sf9</t> cells. Protein size of recombinant Se-AQP was ~32 kDa. It was captured by V5 antibody. ( B ) Immunofluorescence assay for the detection of transient expression of Se-AQP in Sf9 cells. F-actin was specifically detected with Alexa Fluor 555 phalloidin while nucleus was stained with DAPI. To check transient expression, anti-V5-FITC antibody was used. ( C ) Expression patterns of Se-AQP in different developmental stages, including egg, first to fifth instar larvae (‘L1–L5’), pupa, and adult. ( D ) Expression patterns in indicated tissues of L5 larvae, including hemocyte (‘HC’), fat body (‘FB’), and gut (‘Gut’). A ribosomal gene RL32 was used as reference gene. Each treatment was replicated three times with independent tissue preparations. Different letters indicate significant differences among means at Type I error = 0.05 (LSD test).
    Sf9 Cell Line, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 7221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sf9 cell line/product/Roche
    Average 93 stars, based on 7221 article reviews
    Price from $9.99 to $1999.99
    sf9 cell line - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua"

    Article Title: An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-41541-2

    Expression profile of Se-AQP . ( A ) Western blot analysis. Se-AQP was transfected into Sf9 cells. Protein size of recombinant Se-AQP was ~32 kDa. It was captured by V5 antibody. ( B ) Immunofluorescence assay for the detection of transient expression of Se-AQP in Sf9 cells. F-actin was specifically detected with Alexa Fluor 555 phalloidin while nucleus was stained with DAPI. To check transient expression, anti-V5-FITC antibody was used. ( C ) Expression patterns of Se-AQP in different developmental stages, including egg, first to fifth instar larvae (‘L1–L5’), pupa, and adult. ( D ) Expression patterns in indicated tissues of L5 larvae, including hemocyte (‘HC’), fat body (‘FB’), and gut (‘Gut’). A ribosomal gene RL32 was used as reference gene. Each treatment was replicated three times with independent tissue preparations. Different letters indicate significant differences among means at Type I error = 0.05 (LSD test).
    Figure Legend Snippet: Expression profile of Se-AQP . ( A ) Western blot analysis. Se-AQP was transfected into Sf9 cells. Protein size of recombinant Se-AQP was ~32 kDa. It was captured by V5 antibody. ( B ) Immunofluorescence assay for the detection of transient expression of Se-AQP in Sf9 cells. F-actin was specifically detected with Alexa Fluor 555 phalloidin while nucleus was stained with DAPI. To check transient expression, anti-V5-FITC antibody was used. ( C ) Expression patterns of Se-AQP in different developmental stages, including egg, first to fifth instar larvae (‘L1–L5’), pupa, and adult. ( D ) Expression patterns in indicated tissues of L5 larvae, including hemocyte (‘HC’), fat body (‘FB’), and gut (‘Gut’). A ribosomal gene RL32 was used as reference gene. Each treatment was replicated three times with independent tissue preparations. Different letters indicate significant differences among means at Type I error = 0.05 (LSD test).

    Techniques Used: Expressing, Western Blot, Transfection, Recombinant, Immunofluorescence, Staining

    2) Product Images from "Characterisation of Human Oestrogen Receptor beta (ERβ) Splice Variants in Neuronal Cells"

    Article Title: Characterisation of Human Oestrogen Receptor beta (ERβ) Splice Variants in Neuronal Cells

    Journal: Journal of neuroendocrinology

    doi: 10.1111/j.1365-2826.2012.02337.x

    Role of phosphoinositide 3-kinase (PI3K) and p38 kinase inhibition on human oestrogen receptor (hER) β splice variant-mediated repression of human AVP (hAVP) promoter activity. SK-N-SH cells were transiently transfected with 0.15 μ g of hAVP-luciferase reporter construct and 0.15 μ g of an expression vector containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH) or 100 nM 17 β -oestradiol (E 2 ), (A) 10 μ M LY 294002 or (B) 10 μ M SB202190 and 100 nM ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P
    Figure Legend Snippet: Role of phosphoinositide 3-kinase (PI3K) and p38 kinase inhibition on human oestrogen receptor (hER) β splice variant-mediated repression of human AVP (hAVP) promoter activity. SK-N-SH cells were transiently transfected with 0.15 μ g of hAVP-luciferase reporter construct and 0.15 μ g of an expression vector containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH) or 100 nM 17 β -oestradiol (E 2 ), (A) 10 μ M LY 294002 or (B) 10 μ M SB202190 and 100 nM ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Techniques Used: Inhibition, Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated arginine vasopressin (AVP) promoter activity before and after deletion of an activator protein-1 (AP-1) site. SK-N-SH cells were transiently transfected with 0.15 μ g of (A) human AVP (hAVP)-luciferase or (B) hAVPΔ611 – 604-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P
    Figure Legend Snippet: Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated arginine vasopressin (AVP) promoter activity before and after deletion of an activator protein-1 (AP-1) site. SK-N-SH cells were transiently transfected with 0.15 μ g of (A) human AVP (hAVP)-luciferase or (B) hAVPΔ611 – 604-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Techniques Used: Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated oestrogen response element (ERE) and activator protein-1 (AP-1) promoter activity. HT-22 cells were transiently transfected with 0.15 μ g of (A) 2x-ERE- or (B) AP-1-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM of E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector controls. *P
    Figure Legend Snippet: Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated oestrogen response element (ERE) and activator protein-1 (AP-1) promoter activity. HT-22 cells were transiently transfected with 0.15 μ g of (A) 2x-ERE- or (B) AP-1-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM of E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector controls. *P

    Techniques Used: Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    3) Product Images from "Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells"

    Article Title: Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells

    Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

    doi: 10.4196/kjpp.2013.17.1.23

    Comparison of transfection efficiency of three programs -DS112, DS113 and CA137- of nucleofection. (A~C) Representative merged images of NSCs nucleofected using program (A), (B) DS113 and (C) CA137 for 48 hours. GFP positive cells are shown in green and DAPI stained nuclei are shown in blue. Scale bar=100 µm. (D) Quantification of the transfected NSCs. The ratio of GFP-positive cells to the total cells was calculated and presented. Quantitative data are shown as mean±S.D. ( ** p
    Figure Legend Snippet: Comparison of transfection efficiency of three programs -DS112, DS113 and CA137- of nucleofection. (A~C) Representative merged images of NSCs nucleofected using program (A), (B) DS113 and (C) CA137 for 48 hours. GFP positive cells are shown in green and DAPI stained nuclei are shown in blue. Scale bar=100 µm. (D) Quantification of the transfected NSCs. The ratio of GFP-positive cells to the total cells was calculated and presented. Quantitative data are shown as mean±S.D. ( ** p

    Techniques Used: Transfection, Staining

    Comparison of transfection efficiency of lipid-mediated transfection, electroporation, nucleofection and retroviral transduction. (A~D) Representative merged images of NSCs transfected with GFP vector for 48 hours using (A) X-tremeGENE9 transfection reagent, (B) NEPA21 electroporator, (C) Amaxa 4D Nucleofector, and (D) retroviral transduction. By immunostaining GFP positive cells are shown in green and nuclei are shown in blue. Scale bar=100 µm. (E) Quantification data of the transfected cells. The ratio of GFP-positive cells to the total cells was calculated. Quantitative data are shown as mean±S.D. ( ** p
    Figure Legend Snippet: Comparison of transfection efficiency of lipid-mediated transfection, electroporation, nucleofection and retroviral transduction. (A~D) Representative merged images of NSCs transfected with GFP vector for 48 hours using (A) X-tremeGENE9 transfection reagent, (B) NEPA21 electroporator, (C) Amaxa 4D Nucleofector, and (D) retroviral transduction. By immunostaining GFP positive cells are shown in green and nuclei are shown in blue. Scale bar=100 µm. (E) Quantification data of the transfected cells. The ratio of GFP-positive cells to the total cells was calculated. Quantitative data are shown as mean±S.D. ( ** p

    Techniques Used: Transfection, Electroporation, Transduction, Plasmid Preparation, Immunostaining

    Transfection efficiency of rat NSCs 48 hours after lipid-mediated transfection. (A, B) Representative merged images of NSCs transfected with (A) lipid alone and (B) lipid with GFP vector. GFP was visualized by immunostaining with anti-GFP primary antibody and Alexa Fluor 488-conjugated secondary antibody (green). Nuclei were visualized by DAPI staining (blue). Scale bar=100 µm. (C) Quantification of the immunostaining data. The ratio of GFP-positive cells to the total cells was calculated and presented. The data are shown as mean±S.D. ( * p
    Figure Legend Snippet: Transfection efficiency of rat NSCs 48 hours after lipid-mediated transfection. (A, B) Representative merged images of NSCs transfected with (A) lipid alone and (B) lipid with GFP vector. GFP was visualized by immunostaining with anti-GFP primary antibody and Alexa Fluor 488-conjugated secondary antibody (green). Nuclei were visualized by DAPI staining (blue). Scale bar=100 µm. (C) Quantification of the immunostaining data. The ratio of GFP-positive cells to the total cells was calculated and presented. The data are shown as mean±S.D. ( * p

    Techniques Used: Transfection, Plasmid Preparation, Immunostaining, Staining

    4) Product Images from "Mouse Lymphoid Cell Line Selected To Have High Immunoglobulin Promoter Activity"

    Article Title: Mouse Lymphoid Cell Line Selected To Have High Immunoglobulin Promoter Activity

    Journal: Molecular and Cellular Biology

    doi:

    Transfection characteristics of the selected cell lines. (A) High-GFP-expressing cells from four rounds of FACS were single-cell cloned and transiently transfected with pGL3 containing four different promoters (H2B-59 + 1, IgH-154 + 35, B29-164 + 32, and mb-1-252 + 48). Cell line 2017, the parental clone 2017-IgH-GFP no. 9, and 70Z/3 were also transfected. The histone H2B promoter was used as a control. pCMV-βGal was cotransfected, and the luciferase/β-Gal ratios for the H2B construct were normalized to 1. The dark bars depict Ig promoter activity. Shaded bars are B29 and mb-1. Lipid-mediated transfection reagents were used. (B) Selected high-GFP cell lines 70Z/3 and 2017 and parent line 2017-IgH-GFP no. 9 were transiently transfected with either the 17.2.25 promoter (gray bars) or the V H 186.2 promoter with the IgH intronic enhancer upstream (white bars). pCMV-βGal was cotransfected, and luciferase/β-Gal ratios are depicted on the y axis. Lipid-mediated transfection reagents were used. (C) Selected cell lines m4, h3, and 70Z/3, and the parental cell line 2017-IgH-GFP no. 9 were transiently transfected with either IgH-154 + 35 (gray bars) or the same construct in which the intronic enhancer had been inserted (white bars) (see Materials and Methods). Lipid-mediated transfection reagents were used. pCMV-βGal was cotransfected as an internal control. Experiments were performed in triplicate, and error bars denote standard deviations. (D) Scanning promoter mutants were transiently transfected into either the parent cell line 2017-IgH-GFP no. 9 (gray bars) or the high-GFP-selected cell line m4 (white bars). Lipid-mediated transfection reagents were used. pCMV-βGal was cotransfected, and luciferase/β-Gal ratios are depicted on the y axis.
    Figure Legend Snippet: Transfection characteristics of the selected cell lines. (A) High-GFP-expressing cells from four rounds of FACS were single-cell cloned and transiently transfected with pGL3 containing four different promoters (H2B-59 + 1, IgH-154 + 35, B29-164 + 32, and mb-1-252 + 48). Cell line 2017, the parental clone 2017-IgH-GFP no. 9, and 70Z/3 were also transfected. The histone H2B promoter was used as a control. pCMV-βGal was cotransfected, and the luciferase/β-Gal ratios for the H2B construct were normalized to 1. The dark bars depict Ig promoter activity. Shaded bars are B29 and mb-1. Lipid-mediated transfection reagents were used. (B) Selected high-GFP cell lines 70Z/3 and 2017 and parent line 2017-IgH-GFP no. 9 were transiently transfected with either the 17.2.25 promoter (gray bars) or the V H 186.2 promoter with the IgH intronic enhancer upstream (white bars). pCMV-βGal was cotransfected, and luciferase/β-Gal ratios are depicted on the y axis. Lipid-mediated transfection reagents were used. (C) Selected cell lines m4, h3, and 70Z/3, and the parental cell line 2017-IgH-GFP no. 9 were transiently transfected with either IgH-154 + 35 (gray bars) or the same construct in which the intronic enhancer had been inserted (white bars) (see Materials and Methods). Lipid-mediated transfection reagents were used. pCMV-βGal was cotransfected as an internal control. Experiments were performed in triplicate, and error bars denote standard deviations. (D) Scanning promoter mutants were transiently transfected into either the parent cell line 2017-IgH-GFP no. 9 (gray bars) or the high-GFP-selected cell line m4 (white bars). Lipid-mediated transfection reagents were used. pCMV-βGal was cotransfected, and luciferase/β-Gal ratios are depicted on the y axis.

    Techniques Used: Transfection, Expressing, FACS, Clone Assay, Luciferase, Construct, Activity Assay

    (A) Cell lines BJA-B, M12, 70Z/3, θ4b, p5424, 2017, and EL-4 were transfected with either the pGL3 backbone (white bars) or IgH-luciferase promoter constructs containing (IgH-154 + 35) (light gray bars) or lacking (IgH-154 + 1) (dark gray bars) the sequences from the transcription initiation site to +35. pCMV-βGal was cotransfected as an internal control. Transfections were performed by electroporation. The luciferase/β-Gal activity ratio is depicted on the y axis. Averages from three replicate experiments are shown. Error bars denote standard deviations. (B) Promoter constructs containing 3-bp mutants spanning the transcription initiation site were electroporated into either the B-cell line BJA-B (white bars) or the nonlymphoid cell line WERI-27 (dark gray bars). The construct name is shown to the right of the sequence for that construct. The full-length (+35) and deletion (+1) constructs were included as controls. pCMV-βGal was cotransfected, and the luciferase/β-Gal activity ratio is shown on the x ). pCMV-βGal was cotransfected as an internal control. pUC18 was used to equalize the mass of the amount of DNA transfected. Transfections were performed by electroporation. The luciferase/β-Gal activity ratio is depicted on the y axis. Error bars denote standard deviations.
    Figure Legend Snippet: (A) Cell lines BJA-B, M12, 70Z/3, θ4b, p5424, 2017, and EL-4 were transfected with either the pGL3 backbone (white bars) or IgH-luciferase promoter constructs containing (IgH-154 + 35) (light gray bars) or lacking (IgH-154 + 1) (dark gray bars) the sequences from the transcription initiation site to +35. pCMV-βGal was cotransfected as an internal control. Transfections were performed by electroporation. The luciferase/β-Gal activity ratio is depicted on the y axis. Averages from three replicate experiments are shown. Error bars denote standard deviations. (B) Promoter constructs containing 3-bp mutants spanning the transcription initiation site were electroporated into either the B-cell line BJA-B (white bars) or the nonlymphoid cell line WERI-27 (dark gray bars). The construct name is shown to the right of the sequence for that construct. The full-length (+35) and deletion (+1) constructs were included as controls. pCMV-βGal was cotransfected, and the luciferase/β-Gal activity ratio is shown on the x ). pCMV-βGal was cotransfected as an internal control. pUC18 was used to equalize the mass of the amount of DNA transfected. Transfections were performed by electroporation. The luciferase/β-Gal activity ratio is depicted on the y axis. Error bars denote standard deviations.

    Techniques Used: Transfection, Luciferase, Construct, Electroporation, Activity Assay, Sequencing

    5) Product Images from "Differential Fibroblast Growth Factor 8 (FGF8)-Mediated Autoregulation of Its Cognate Receptors, Fgfr1 and Fgfr3, in Neuronal Cell Lines"

    Article Title: Differential Fibroblast Growth Factor 8 (FGF8)-Mediated Autoregulation of Its Cognate Receptors, Fgfr1 and Fgfr3, in Neuronal Cell Lines

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0010143

    Effects of FGF8b on GnRH promoter activity. Transient transfection of GT1-7 cells with 0.15 µg/well of mouse full-length GnRH-luciferase reporter construct. Following transfection, cells were treated with vehicle, FGF8b (50 ng/ml), PD173074 (100 nM), or FGF8b + PD173074 for 8 hours. Data are represented as mean percent change in RLU's from vehicle-treated controls ± SEM. Dissimilar letters indicate statistically significant difference among groups, P
    Figure Legend Snippet: Effects of FGF8b on GnRH promoter activity. Transient transfection of GT1-7 cells with 0.15 µg/well of mouse full-length GnRH-luciferase reporter construct. Following transfection, cells were treated with vehicle, FGF8b (50 ng/ml), PD173074 (100 nM), or FGF8b + PD173074 for 8 hours. Data are represented as mean percent change in RLU's from vehicle-treated controls ± SEM. Dissimilar letters indicate statistically significant difference among groups, P

    Techniques Used: Activity Assay, Transfection, Luciferase, Construct

    Related Articles

    Electroporation:

    Article Title: Human Parvovirus B19 Nonstructural Protein (NS1) Induces Cell Cycle Arrest at G1 Phase
    Article Snippet: .. Transient transfections to 293T cells were performed with FuGene 6 (Roche Diagnostics, Indianapolis, Ind.) and to UT7/Epo-S1 cells were performed with “amaxa” electroporation gene transfer tool (Amaxa GmbH, Berlin, Germany). ..

    Transfection:

    Article Title: LRP6 Protein Regulates Low Density Lipoprotein (LDL) Receptor-mediated LDL Uptake
    Article Snippet: .. Cell transfections were carried out with FuGENE 6 (Roche Applied Science) as the per manufacturer's instructions. .. Cell lysates (2 mg) were subjected to overnight immunoprecipitation with HA antibody in the presence of protein A/G-Sepharose beads at 4 °C.

    Article Title: A classical NLS and the SUN domain contribute to the targeting of SUN2 to the inner nuclear membrane
    Article Snippet: .. Transient transfections were performed using FuGene transfection reagent (Roche). .. Cells were fixed 20–36 h after transfection with 4% PFA for 10 min. After washing with PBS, cover slips were mounted in VectaShield (VectorLabs) for microscopic analysis.

    Article Title: A Haplotype of Angiotensin Receptor Type 1 Associated with Human Hypertension Increases Blood Pressure in Transgenic Mice *
    Article Snippet: .. Transient transfections were performed with FuGENE 6 transfection reagent (Roche Applied Science) following the manufacturer's protocol. .. For co-transfection experiments, expression vector pSV-USF2 (100 ng) was added to the reporter constructs.

    Article Title: Human Parvovirus B19 Nonstructural Protein (NS1) Induces Cell Cycle Arrest at G1 Phase
    Article Snippet: .. Transient transfections to 293T cells were performed with FuGene 6 (Roche Diagnostics, Indianapolis, Ind.) and to UT7/Epo-S1 cells were performed with “amaxa” electroporation gene transfer tool (Amaxa GmbH, Berlin, Germany). ..

    Article Title: The miRNA let-7a1 inhibits the expression of insulin-like growth factor 1 receptor (IGF1R) in prostate cancer PC-3 cells
    Article Snippet: .. Cell transfections were performed using FuGENE® HD (Roche, Mannheim, Germany) or siPORT NeoFX Transfection Agent (Ambion) according to the manufacturer's instructions. .. For RT-PCR and Western blot analysis, the cells were grown in 6-well plates and transfected for 48 h with either pSilencer4.1- let7a 1 or parental vector, with either let-7a 1 inhibitor or normal control (NC) inhibitor (Ambion).

    Article Title: Microsatellite-encoded domain in rodent Sry functions as a genetic capacitor to enable the rapid evolution of biological novelty
    Article Snippet: .. Transient transfections were carried out by the Fugene HD protocol (Hoffmann LaRoche). .. PCR primers were in accordance with human genomic sequences.

    Article Title: An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua
    Article Snippet: .. Heterologous expression of Se-AQP in Sf9 cells Using an eukaryotic expression vector pIB/V5-His (Invitrogen), a recombinant pIB/V5-His-Se-AQP was prepared and transiently expressed in Sf9 cell line by cationic lipid-mediated transfection using X-treme GENE 9 DNA transfection reagent (Roche, Mannheim, Germany). .. Transfection procedure and extraction of cellular proteins from Sf9 cells were performed according to the method described by Kumar and Kim and quantified using Bradford method .

    Article Title: Np9 Protein of Human Endogenous Retrovirus K Interacts with Ligand of Numb Protein X
    Article Snippet: .. Transient transfections of Cos-1 cell cultures with FuGene-6 (Roche) routinely produced 70 to 80% transfected cells. ..

    Produced:

    Article Title: Np9 Protein of Human Endogenous Retrovirus K Interacts with Ligand of Numb Protein X
    Article Snippet: .. Transient transfections of Cos-1 cell cultures with FuGene-6 (Roche) routinely produced 70 to 80% transfected cells. ..

    Expressing:

    Article Title: An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua
    Article Snippet: .. Heterologous expression of Se-AQP in Sf9 cells Using an eukaryotic expression vector pIB/V5-His (Invitrogen), a recombinant pIB/V5-His-Se-AQP was prepared and transiently expressed in Sf9 cell line by cationic lipid-mediated transfection using X-treme GENE 9 DNA transfection reagent (Roche, Mannheim, Germany). .. Transfection procedure and extraction of cellular proteins from Sf9 cells were performed according to the method described by Kumar and Kim and quantified using Bradford method .

    Recombinant:

    Article Title: An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua
    Article Snippet: .. Heterologous expression of Se-AQP in Sf9 cells Using an eukaryotic expression vector pIB/V5-His (Invitrogen), a recombinant pIB/V5-His-Se-AQP was prepared and transiently expressed in Sf9 cell line by cationic lipid-mediated transfection using X-treme GENE 9 DNA transfection reagent (Roche, Mannheim, Germany). .. Transfection procedure and extraction of cellular proteins from Sf9 cells were performed according to the method described by Kumar and Kim and quantified using Bradford method .

    Plasmid Preparation:

    Article Title: An aquaporin mediates cell shape change required for cellular immunity in the beet armyworm, Spodoptera exigua
    Article Snippet: .. Heterologous expression of Se-AQP in Sf9 cells Using an eukaryotic expression vector pIB/V5-His (Invitrogen), a recombinant pIB/V5-His-Se-AQP was prepared and transiently expressed in Sf9 cell line by cationic lipid-mediated transfection using X-treme GENE 9 DNA transfection reagent (Roche, Mannheim, Germany). .. Transfection procedure and extraction of cellular proteins from Sf9 cells were performed according to the method described by Kumar and Kim and quantified using Bradford method .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Roche lipid mediated transfection reagent
    Role of phosphoinositide 3-kinase (PI3K) and p38 kinase inhibition on human oestrogen receptor (hER) β splice variant-mediated repression of human AVP (hAVP) promoter activity. SK-N-SH cells were transiently transfected with 0.15 μ g of hAVP-luciferase reporter construct and 0.15 μ g of an expression vector containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post <t>transfection,</t> cells were treated with vehicle (0.001% EtOH) or 100 nM 17 β -oestradiol (E 2 ), (A) 10 μ M LY 294002 or (B) 10 μ M SB202190 and 100 nM ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P
    Lipid Mediated Transfection Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid mediated transfection reagent/product/Roche
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lipid mediated transfection reagent - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Role of phosphoinositide 3-kinase (PI3K) and p38 kinase inhibition on human oestrogen receptor (hER) β splice variant-mediated repression of human AVP (hAVP) promoter activity. SK-N-SH cells were transiently transfected with 0.15 μ g of hAVP-luciferase reporter construct and 0.15 μ g of an expression vector containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH) or 100 nM 17 β -oestradiol (E 2 ), (A) 10 μ M LY 294002 or (B) 10 μ M SB202190 and 100 nM ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Journal: Journal of neuroendocrinology

    Article Title: Characterisation of Human Oestrogen Receptor beta (ERβ) Splice Variants in Neuronal Cells

    doi: 10.1111/j.1365-2826.2012.02337.x

    Figure Lengend Snippet: Role of phosphoinositide 3-kinase (PI3K) and p38 kinase inhibition on human oestrogen receptor (hER) β splice variant-mediated repression of human AVP (hAVP) promoter activity. SK-N-SH cells were transiently transfected with 0.15 μ g of hAVP-luciferase reporter construct and 0.15 μ g of an expression vector containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH) or 100 nM 17 β -oestradiol (E 2 ), (A) 10 μ M LY 294002 or (B) 10 μ M SB202190 and 100 nM ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Article Snippet: Transfections were carried out using a lipid-mediated transfection reagent in accordance with the manufacturer’s instructions (Fugene6; Roche Molecular Biomedical, Indianapolis, IN, USA).

    Techniques: Inhibition, Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated arginine vasopressin (AVP) promoter activity before and after deletion of an activator protein-1 (AP-1) site. SK-N-SH cells were transiently transfected with 0.15 μ g of (A) human AVP (hAVP)-luciferase or (B) hAVPΔ611 – 604-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Journal: Journal of neuroendocrinology

    Article Title: Characterisation of Human Oestrogen Receptor beta (ERβ) Splice Variants in Neuronal Cells

    doi: 10.1111/j.1365-2826.2012.02337.x

    Figure Lengend Snippet: Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated arginine vasopressin (AVP) promoter activity before and after deletion of an activator protein-1 (AP-1) site. SK-N-SH cells were transiently transfected with 0.15 μ g of (A) human AVP (hAVP)-luciferase or (B) hAVPΔ611 – 604-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector, vehicle-treated controls. *P

    Article Snippet: Transfections were carried out using a lipid-mediated transfection reagent in accordance with the manufacturer’s instructions (Fugene6; Roche Molecular Biomedical, Indianapolis, IN, USA).

    Techniques: Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated oestrogen response element (ERE) and activator protein-1 (AP-1) promoter activity. HT-22 cells were transiently transfected with 0.15 μ g of (A) 2x-ERE- or (B) AP-1-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM of E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector controls. *P

    Journal: Journal of neuroendocrinology

    Article Title: Characterisation of Human Oestrogen Receptor beta (ERβ) Splice Variants in Neuronal Cells

    doi: 10.1111/j.1365-2826.2012.02337.x

    Figure Lengend Snippet: Effects of 17 β -oestradiol (E 2 ), 17 β -diol (3 β -diol) and ICI 182 780 on human oestrogen receptor (hER) β splice variant-mediated oestrogen response element (ERE) and activator protein-1 (AP-1) promoter activity. HT-22 cells were transiently transfected with 0.15 μ g of (A) 2x-ERE- or (B) AP-1-luciferase reporter constructs and 0.15 μ g of expression vectors containing hER β 1, hER β 2, hER β 4 or hER β 5. Twenty-four hours post transfection, cells were treated with vehicle (0.001% EtOH), 100 nM of E 2 , 3 β -diol or ICI 182 780 for 12 h. Data represent the percentage change in relative light units compared to empty vector controls. *P

    Article Snippet: Transfections were carried out using a lipid-mediated transfection reagent in accordance with the manufacturer’s instructions (Fugene6; Roche Molecular Biomedical, Indianapolis, IN, USA).

    Techniques: Variant Assay, Activity Assay, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation

    Effects of FGF8b on GnRH promoter activity. Transient transfection of GT1-7 cells with 0.15 µg/well of mouse full-length GnRH-luciferase reporter construct. Following transfection, cells were treated with vehicle, FGF8b (50 ng/ml), PD173074 (100 nM), or FGF8b + PD173074 for 8 hours. Data are represented as mean percent change in RLU's from vehicle-treated controls ± SEM. Dissimilar letters indicate statistically significant difference among groups, P

    Journal: PLoS ONE

    Article Title: Differential Fibroblast Growth Factor 8 (FGF8)-Mediated Autoregulation of Its Cognate Receptors, Fgfr1 and Fgfr3, in Neuronal Cell Lines

    doi: 10.1371/journal.pone.0010143

    Figure Lengend Snippet: Effects of FGF8b on GnRH promoter activity. Transient transfection of GT1-7 cells with 0.15 µg/well of mouse full-length GnRH-luciferase reporter construct. Following transfection, cells were treated with vehicle, FGF8b (50 ng/ml), PD173074 (100 nM), or FGF8b + PD173074 for 8 hours. Data are represented as mean percent change in RLU's from vehicle-treated controls ± SEM. Dissimilar letters indicate statistically significant difference among groups, P

    Article Snippet: Transfections were carried out using a lipid-mediated transfection reagent according to manufacturer's instructions (Fugene6, Roche Molecular Biomedical, Indianapolis, IN).

    Techniques: Activity Assay, Transfection, Luciferase, Construct