Structured Review

Horizon Discovery lipid mediated dharmafect transfection reagent
Lipid Mediated Dharmafect Transfection Reagent, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipid mediated dharmafect transfection reagent/product/Horizon Discovery
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
lipid mediated dharmafect transfection reagent - by Bioz Stars, 2020-09
85/100 stars

Related Products / Commonly Used Together

mnsod-sirna smartpool
si-foxo3a

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Related Articles

Concentration Assay:

Article Title: Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice
Article Snippet: .. After 24 hours, the MnSOD-siRNA SMARTpool (si-MnSOD, Dharmacon) was diluted to a 2 μM working solution and delivered to cells at 100 nM final concentration for 72 hours through a lipid-mediated DharmaFECT Transfection Reagent (Dharmacon). .. A non-related scramble siRNA (Dharmacon) was used as a transfection control.

Article Title: AGE-RELATED DIFFERENCES IN INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR SIGNALING REGULATES AKT/FOXO3A AND ERK/FOS PATHWAYS IN VASCULAR SMOOTH MUSCLE CELLS
Article Snippet: .. After 24 h, the si-FOXO3a was diluted to a 2 μM working solution and delivered to cells at 100 nM final concentration for 48 hours through a lipid-mediated DharmaFECT Transfection Reagent (Dharmacon). .. A siCONTROL Cyclophilin B siRNA (Dharmacon) was also included as a transfection control.

Transfection:

Article Title: Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice
Article Snippet: .. After 24 hours, the MnSOD-siRNA SMARTpool (si-MnSOD, Dharmacon) was diluted to a 2 μM working solution and delivered to cells at 100 nM final concentration for 72 hours through a lipid-mediated DharmaFECT Transfection Reagent (Dharmacon). .. A non-related scramble siRNA (Dharmacon) was used as a transfection control.

Article Title: AGE-RELATED DIFFERENCES IN INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR SIGNALING REGULATES AKT/FOXO3A AND ERK/FOS PATHWAYS IN VASCULAR SMOOTH MUSCLE CELLS
Article Snippet: .. After 24 h, the si-FOXO3a was diluted to a 2 μM working solution and delivered to cells at 100 nM final concentration for 48 hours through a lipid-mediated DharmaFECT Transfection Reagent (Dharmacon). .. A siCONTROL Cyclophilin B siRNA (Dharmacon) was also included as a transfection control.

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    Horizon Discovery dharmafect sirna transfection protocol
    AR coregulatory gene downregulation and decrease in PCa cancer cell viability are shRNA seed mediated. ( A ) Relative percent viability and TMEFF2 mRNA expression in LNCaP cells transfected with siTMEFF2-4 and control siRNAs (siNon-Target; Negative seed controls: siTMEFF2-4+3, siTMEFF2-4+5, 5p-siTMEFF2-4; Positive seed control: si634). 5p designates an ON-Target-Plus modification (Dharmacon) that blocks seed mediated gene downregulation. Cell viability was determined by trypan blue. mRNA expression was determined by RT qPCR using RPL8, RPL38, PSMA1 and PPP2CA housekeeping genes for normalization. Viability measurements, cell pictures and RNA extractions were done 72 hours after <t>siRNA</t> <t>transfections</t> N=4, error bars ±SD, * p
    Dharmafect Sirna Transfection Protocol, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dharmafect sirna transfection protocol/product/Horizon Discovery
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dharmafect sirna transfection protocol - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    92
    Horizon Discovery dharmafect 4 transfection reagent
    Silencing NDRG1 in SUM-159 and MDA-MB-231 cells. (A) Western blot analysis of lysates from MDA-MB-231 and SUM-159 cell lines transfected with NDRG1 siRNA at 48, 72, 96, and 120 hours post-transfection. (B) Transfection after 48 hours was validated by immunocytochemistry for depleted NDRG1 protein (brown) in MDA-MB-231 and SUM-159 cells. Blank control represents non-transfected control cells; transfection reagent represents cells in the presence of <t>DharmaFECT</t> 4 transfection reagent only; negative control represents cells transfected with non-targeting control siRNA. (C) Proliferation of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined using the SRB assay at 120 and 72 hours, respectively, post-transfection with NDRG1 siRNA or non-targeting control siRNA. Experiments were performed in triplicate, and data were normalised to control (untreated) cells; statistical significance was computed using 1-way ANOVA. (D) Migration rate of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined by wound healing assay 48 hours after transfection. Relative migration was determined after 24 hours. Data were normalised to control cells; statistical significance was computed using 1-way ANOVA. ANOVA indicates analysis of variance; SRB, sulforhodamine B.
    Dharmafect 4 Transfection Reagent, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dharmafect 4 transfection reagent/product/Horizon Discovery
    Average 92 stars, based on 499 article reviews
    Price from $9.99 to $1999.99
    dharmafect 4 transfection reagent - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    AR coregulatory gene downregulation and decrease in PCa cancer cell viability are shRNA seed mediated. ( A ) Relative percent viability and TMEFF2 mRNA expression in LNCaP cells transfected with siTMEFF2-4 and control siRNAs (siNon-Target; Negative seed controls: siTMEFF2-4+3, siTMEFF2-4+5, 5p-siTMEFF2-4; Positive seed control: si634). 5p designates an ON-Target-Plus modification (Dharmacon) that blocks seed mediated gene downregulation. Cell viability was determined by trypan blue. mRNA expression was determined by RT qPCR using RPL8, RPL38, PSMA1 and PPP2CA housekeeping genes for normalization. Viability measurements, cell pictures and RNA extractions were done 72 hours after siRNA transfections N=4, error bars ±SD, * p

    Journal: bioRxiv

    Article Title: SEED-MEDIATED RNA INTERFERENCE OF ANDROGEN SIGNALING AND SURVIVAL NETWORKS INDUCES CELL DEATH IN PROSTATE CANCER CELLS

    doi: 10.1101/2020.07.17.209379

    Figure Lengend Snippet: AR coregulatory gene downregulation and decrease in PCa cancer cell viability are shRNA seed mediated. ( A ) Relative percent viability and TMEFF2 mRNA expression in LNCaP cells transfected with siTMEFF2-4 and control siRNAs (siNon-Target; Negative seed controls: siTMEFF2-4+3, siTMEFF2-4+5, 5p-siTMEFF2-4; Positive seed control: si634). 5p designates an ON-Target-Plus modification (Dharmacon) that blocks seed mediated gene downregulation. Cell viability was determined by trypan blue. mRNA expression was determined by RT qPCR using RPL8, RPL38, PSMA1 and PPP2CA housekeeping genes for normalization. Viability measurements, cell pictures and RNA extractions were done 72 hours after siRNA transfections N=4, error bars ±SD, * p

    Article Snippet: Dharmafect siRNA transfection protocol was used for transfections, ( https://horizondiscovery.com/-/media/Files/Horizon/resources/Protocols/basic-dharmafect-protocol.pdf).Dharmafect reagent #1 (0.2%) was used for RWPE1, BHPre1 and NHPre1 cell lines, and 0.2% Dharmafect reagent #3 was used for LNCaP, C4-2B and 22Rv1 cell lines.

    Techniques: shRNA, Expressing, Transfection, Modification, Quantitative RT-PCR

    AN-DISE siRNAs do not reduce the viability of normal prostate epithelial cell lines. Relative percent viability of normal prostate (RWPE1, BHPre1, NHPre1) and PCa cell lines (LNCaP, C4-2B and 22Rv1) transfected with Cy5 labeled siNon-Target, siTMEFF2-4 or siTMEFF2-9 siRNAs. Viability was determined by trypan blue 72 hours after siRNA transfection. N=3, error bars ±SD, * p

    Journal: bioRxiv

    Article Title: SEED-MEDIATED RNA INTERFERENCE OF ANDROGEN SIGNALING AND SURVIVAL NETWORKS INDUCES CELL DEATH IN PROSTATE CANCER CELLS

    doi: 10.1101/2020.07.17.209379

    Figure Lengend Snippet: AN-DISE siRNAs do not reduce the viability of normal prostate epithelial cell lines. Relative percent viability of normal prostate (RWPE1, BHPre1, NHPre1) and PCa cell lines (LNCaP, C4-2B and 22Rv1) transfected with Cy5 labeled siNon-Target, siTMEFF2-4 or siTMEFF2-9 siRNAs. Viability was determined by trypan blue 72 hours after siRNA transfection. N=3, error bars ±SD, * p

    Article Snippet: Dharmafect siRNA transfection protocol was used for transfections, ( https://horizondiscovery.com/-/media/Files/Horizon/resources/Protocols/basic-dharmafect-protocol.pdf).Dharmafect reagent #1 (0.2%) was used for RWPE1, BHPre1 and NHPre1 cell lines, and 0.2% Dharmafect reagent #3 was used for LNCaP, C4-2B and 22Rv1 cell lines.

    Techniques: Transfection, Labeling

    Reduction in PCa cancer cell viability is seed mediated. ( A/B ) Relative percent viability and TMEFF2 mRNA expression in LNCaP cells transfected with siTMEFF2-9 ( A ) or siTMEFF2-3 ( B ) and control siRNAs (siNon-Target; Negative seed control: 5p-siTMEFF2). 5p designates an ON-Target-Plus modification (Dharmacon) that blocks seed mediated gene downregulation. Cell viability was determined by trypan blue. Viability measurements, cell pictures and RNA extractions were done 72 hours after siRNA transfections. mRNA expression was determined by RT qPCR using RPL8, RPL38, PSMA1 and PPP2CA housekeeping genes for normalization. N=4, error bars ±SD, * p

    Journal: bioRxiv

    Article Title: SEED-MEDIATED RNA INTERFERENCE OF ANDROGEN SIGNALING AND SURVIVAL NETWORKS INDUCES CELL DEATH IN PROSTATE CANCER CELLS

    doi: 10.1101/2020.07.17.209379

    Figure Lengend Snippet: Reduction in PCa cancer cell viability is seed mediated. ( A/B ) Relative percent viability and TMEFF2 mRNA expression in LNCaP cells transfected with siTMEFF2-9 ( A ) or siTMEFF2-3 ( B ) and control siRNAs (siNon-Target; Negative seed control: 5p-siTMEFF2). 5p designates an ON-Target-Plus modification (Dharmacon) that blocks seed mediated gene downregulation. Cell viability was determined by trypan blue. Viability measurements, cell pictures and RNA extractions were done 72 hours after siRNA transfections. mRNA expression was determined by RT qPCR using RPL8, RPL38, PSMA1 and PPP2CA housekeeping genes for normalization. N=4, error bars ±SD, * p

    Article Snippet: Dharmafect siRNA transfection protocol was used for transfections, ( https://horizondiscovery.com/-/media/Files/Horizon/resources/Protocols/basic-dharmafect-protocol.pdf).Dharmafect reagent #1 (0.2%) was used for RWPE1, BHPre1 and NHPre1 cell lines, and 0.2% Dharmafect reagent #3 was used for LNCaP, C4-2B and 22Rv1 cell lines.

    Techniques: Expressing, Transfection, Modification, Quantitative RT-PCR

    Silencing NDRG1 in SUM-159 and MDA-MB-231 cells. (A) Western blot analysis of lysates from MDA-MB-231 and SUM-159 cell lines transfected with NDRG1 siRNA at 48, 72, 96, and 120 hours post-transfection. (B) Transfection after 48 hours was validated by immunocytochemistry for depleted NDRG1 protein (brown) in MDA-MB-231 and SUM-159 cells. Blank control represents non-transfected control cells; transfection reagent represents cells in the presence of DharmaFECT 4 transfection reagent only; negative control represents cells transfected with non-targeting control siRNA. (C) Proliferation of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined using the SRB assay at 120 and 72 hours, respectively, post-transfection with NDRG1 siRNA or non-targeting control siRNA. Experiments were performed in triplicate, and data were normalised to control (untreated) cells; statistical significance was computed using 1-way ANOVA. (D) Migration rate of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined by wound healing assay 48 hours after transfection. Relative migration was determined after 24 hours. Data were normalised to control cells; statistical significance was computed using 1-way ANOVA. ANOVA indicates analysis of variance; SRB, sulforhodamine B.

    Journal: Breast Cancer : Basic and Clinical Research

    Article Title: Evaluation of Gene Expression Data From Cybrids and Tumours Highlights Elevated NDRG1-Driven Proliferation in Triple-Negative Breast Cancer

    doi: 10.1177/1178223420934447

    Figure Lengend Snippet: Silencing NDRG1 in SUM-159 and MDA-MB-231 cells. (A) Western blot analysis of lysates from MDA-MB-231 and SUM-159 cell lines transfected with NDRG1 siRNA at 48, 72, 96, and 120 hours post-transfection. (B) Transfection after 48 hours was validated by immunocytochemistry for depleted NDRG1 protein (brown) in MDA-MB-231 and SUM-159 cells. Blank control represents non-transfected control cells; transfection reagent represents cells in the presence of DharmaFECT 4 transfection reagent only; negative control represents cells transfected with non-targeting control siRNA. (C) Proliferation of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined using the SRB assay at 120 and 72 hours, respectively, post-transfection with NDRG1 siRNA or non-targeting control siRNA. Experiments were performed in triplicate, and data were normalised to control (untreated) cells; statistical significance was computed using 1-way ANOVA. (D) Migration rate of SUM-159 (top) and MDA-MB-231 (bottom) cells was determined by wound healing assay 48 hours after transfection. Relative migration was determined after 24 hours. Data were normalised to control cells; statistical significance was computed using 1-way ANOVA. ANOVA indicates analysis of variance; SRB, sulforhodamine B.

    Article Snippet: NDRG1 siRNA was Dharmacon SMARTpool ON-TARGETplus (L-010563-00-0005, https://horizondiscovery.com/products/gene-modulation/knockdown-reagents/sirna/PIFs/ON-TARGETplus-siRNA-Reagents-Human?nodeid=entrezgene-10397), the ON-TARGETplus non-targeting siRNA (D-001810-01-05) and DharmaFECT 4 transfection reagent (T-2004-02) were from Horizon Discovery.

    Techniques: Multiple Displacement Amplification, Western Blot, Transfection, Immunocytochemistry, Negative Control, Sulforhodamine B Assay, Migration, Wound Healing Assay