linear trap quadrupole ltq orbitrapxl mass spectrometer  (Thermo Fisher)


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    Name:
    LTQ XL Linear Ion Trap Mass Spectrometer
    Description:
    Obtain more structural information for proteomics and metabolism applications The Thermo Scientific LTQ XL ion trap mass spectrometer offers multiple dissociation techniques PQD ETD and CID for routine structural elucidation PQD is a proprietary technique that eliminates low mass cut off concerns The result is more extensive coverage for predicted and unpredicted metabolites and the ability to perform peptide quantification using isobaric labels
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    iqlaaegaavfaczmaik
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    Industrial & Applied Science|Industrial Mass Spectrometry
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    Structured Review

    Thermo Fisher linear trap quadrupole ltq orbitrapxl mass spectrometer
    Obtain more structural information for proteomics and metabolism applications The Thermo Scientific LTQ XL ion trap mass spectrometer offers multiple dissociation techniques PQD ETD and CID for routine structural elucidation PQD is a proprietary technique that eliminates low mass cut off concerns The result is more extensive coverage for predicted and unpredicted metabolites and the ability to perform peptide quantification using isobaric labels
    https://www.bioz.com/result/linear trap quadrupole ltq orbitrapxl mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    linear trap quadrupole ltq orbitrapxl mass spectrometer - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Discovering and validating unknown phospho-sites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools
    Article Snippet: .. In fact, multistage activation resulted in more information for the suite of phosphopeptides studied (Table ) (see an example of the spectrum of an identified phosphorylated peptide when using SIMAC coupled to MSA in the LTQ ion Trap mass spectrometer and Mascot, Figure ). ..

    Mass Spectrometry:

    Article Title: Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris
    Article Snippet: .. A reversed-phase LC directly coupled to a LTQ ion trap mass spectrometer (Thermo Electron, Waltham, MA) was run using a linear gradient elution from buffer A (H2 O plus 0.1% formic acid) to 50% buffer A plus 50% buffer B (acetonitrile plus 0.1% formic acid) in 100 min. ..

    Article Title: Quantitative in vivo Analyses Reveal Calcium-dependent Phosphorylation Sites and Identifies a Novel Component of the Toxoplasma Invasion Motor Complex
    Article Snippet: .. Individual TiO2 -bound phosphopeptide fractions were analyzed by multi-dimensional LC-MS/MS on an LTQ linear ion trap mass spectrometer (Thermo Scientific), according to published protocols . ..

    Article Title: High Throughput Characterization of Combinatorial Histone Codes *
    Article Snippet: .. Several other buffer systems for the B mobile phase were tried during method development as noted under “Results.” The column eluent was introduced into an LTQ-ETD ion trap mass spectrometer (Thermo Scientific, Waltham, MA) or an LTQ-Orbitrap XL (Thermo Scientific) (data in only) by nanoelectrospray ionization. .. Every cycle a full mass spectrum was acquired from 300 to 2000 m/z followed by narrower mass range full mass spectrum to select a given charge state of the histone for data-dependent selection.

    Article Title: Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
    Article Snippet: .. UHPLC-LTQ-XL-IT-MS/MS analysis The Thermo Fischer Scientific LTQ XL linear ion trap mass spectrometry system used in the present study consisted of an electrospray interface (Thermo Fischer Scientific, San José, CA, USA) coupled with a DIONEX UltiMate 3000 RS Pump, RS Autosampler, RS Column Compartment, and RS Diode Array Detector (Dionex Corporation, Sunnyvale, CA, USA). .. The samples were separated on a Thermo Scientific Syncronis C18 UHPLC column with 1.7 μm particle size.

    Article Title: Discovering and validating unknown phospho-sites from p38 and HuR protein kinases in vitro by Phosphoproteomic and Bioinformatic tools
    Article Snippet: .. In fact, multistage activation resulted in more information for the suite of phosphopeptides studied (Table ) (see an example of the spectrum of an identified phosphorylated peptide when using SIMAC coupled to MSA in the LTQ ion Trap mass spectrometer and Mascot, Figure ). ..

    Article Title: Ultrahigh Pressure Processing Produces Alterations in the Metabolite Profiles of Panax ginseng
    Article Snippet: .. UHPLC-LTQ-IT-MS/MS Analysis The Thermo Fisher Scientific LTQ XL ion trap mass spectrometer consisted of an electrospray interface (Thermo Fisher Scientific, San Jose, CA, USA) coupled with a DIONEX UltiMate 3000 RS Pump, RS autosampler, RS column compartment, and RS diode array detector (Dionex Corporation, Sunnyvale, CA, USA). .. Each 10 μL sample was injected into and separated on a Thermo Scientific Syncronis C18 UHPLC column (100 mm × 2.1 mm i.d.

    Article Title: Increased expression of the high-mannose M6N2 and NeuAc3H3N3M3N2F tri-antennary N-glycans in cholangiocarcinoma
    Article Snippet: .. Briefly, permethylated glycans were dissolved in 1 mM NaOH in 50% methanol and infused directly into a linear ion trap mass spectrometer (LTQ Orbitrap Discovery; Thermo Fisher Scientific, Inc., Waltham, MA, USA) using a Thermo Fisher Scientific™ nanospray ion source (Thermo Fisher Scientific, Inc.). .. MS analysis was performed in a positive ion mode and MS/MS spectra (at 28% collision energy) were obtained using the total ion mapping function of the Xcalibur software (version 2; Thermo Fisher Scientific, Inc.).

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery
    Article Snippet: .. The resulting peptides were analysed by nanoflow LC-MS/MS (nanoLC-MS/MS) using a LTQ-XL ion-trap mass spectrometer (Thermo, CA, USA). ..

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    Thermo Fisher maldi linear trap quadrupole ltq orbitrap xl mass spectrometer
    CID fragment ions of linearized S-entianin ([A + H] + = 3,303.9 Da) detected with the linear ion trap of a <t>MALDI</t> <t>LTQ</t> <t>Orbitrap</t> XL mass spectrometer. For better representation of less-intense signals, the lower m/z range of the spectrum
    Maldi Linear Trap Quadrupole Ltq Orbitrap Xl Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maldi linear trap quadrupole ltq orbitrap xl mass spectrometer/product/Thermo Fisher
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    maldi linear trap quadrupole ltq orbitrap xl mass spectrometer - by Bioz Stars, 2020-07
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    85
    Thermo Fisher linear quadrupole ion trap ltq orbitrap mass spectrometer
    Workflow to identify lipid raft-associated proteins. Stable isotopic labeling of amino acids in cell culture (SILAC) labeled SH-SY5Y cells left untreated or exposed to 400 nM okadaic acid for 50 min before mixed in a ratio 1:1 in 0.7% Triton X-100/MES buffer. After centrifugation in a sucrose gradient, 10 fractions of 0.5 mL were collected. The lipid raft fractions were identified by Western blotting using flotillin as a lipid raft marker. Fractions containing flotillin were pooled prior to removal of lipids by chloroform/methanol extraction, followed by acetone precipitation. Proteins were separated by SDS-PAGE. Proteins were in-gel digested with trypsin, and peptides were purified either with (1) C18 or (2) IMAC/TiO 2 and analyzed on <t>LTQ-Orbitrap.</t> Proteins were identified with Mascot (ver. 2.2) and quantified using MaxQuant (ver. 1.13).
    Linear Quadrupole Ion Trap Ltq Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear quadrupole ion trap ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    linear quadrupole ion trap ltq orbitrap mass spectrometer - by Bioz Stars, 2020-07
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    89
    Thermo Fisher linear trap quadrupole ltq orbitrap velos mass spectrometer
    Analysis of interaction dynamics by QUBIC triple SILAC based quantitative mass spectrometry. (A) Experimental workflow for triple SILAC pull-downs to determine Wnt3a-dependent interaction dynamics. The cell line expressing the GFP-tagged protein of interest is light and medium SILAC labeled, the untransfected wild-type control cell line is heavy SILAC labeled. Cells are lysed after two hour treatment with Wnt3a (200 ng/mL) or vehicle solution respectively. GFP-pull-downs are performed separately for each SILAC state. Eluates are combined, separated on a one-dimensional gel into eight slices and in-gel digested. Resulting peptide mixtures are analyzed by high resolution LC-MS/MS on an <t>LTQ-Orbitrap</t> <t>Velos.</t> SILAC ratios are automatically quantified by MaxQuant. (B) SILAC peptide triplets representing peak profiles characteristic of background, constitutive and dynamic binders. (C) Data analysis plot of the ratio representing interaction specificity versus the ratio representing stimulus specificity of the interaction. Filled dots represent significant interactors and of these, constitutive interactors are depicted in blue. Dynamic interactors with enhanced binding to the bait protein are shown in red and those with reduced binding to the bait protein in green.
    Linear Trap Quadrupole Ltq Orbitrap Velos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear trap quadrupole ltq orbitrap velos mass spectrometer/product/Thermo Fisher
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    linear trap quadrupole ltq orbitrap velos mass spectrometer - by Bioz Stars, 2020-07
    89/100 stars
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    Image Search Results


    CID fragment ions of linearized S-entianin ([A + H] + = 3,303.9 Da) detected with the linear ion trap of a MALDI LTQ Orbitrap XL mass spectrometer. For better representation of less-intense signals, the lower m/z range of the spectrum

    Journal: Applied and Environmental Microbiology

    Article Title: Entianin, a Novel Subtilin-Like Lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with High Antimicrobial Activity ▿ with High Antimicrobial Activity ▿ †

    doi: 10.1128/AEM.01962-10

    Figure Lengend Snippet: CID fragment ions of linearized S-entianin ([A + H] + = 3,303.9 Da) detected with the linear ion trap of a MALDI LTQ Orbitrap XL mass spectrometer. For better representation of less-intense signals, the lower m/z range of the spectrum

    Article Snippet: For all mass spectrometric analyses, a Voyager- DE STR mass spectrometer (Applied Biosystems, Darmstadt, Germany) and a MALDI linear trap quadrupole (LTQ) Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Inc., Waltham, MA) were used.

    Techniques: Mass Spectrometry

    Workflow to identify lipid raft-associated proteins. Stable isotopic labeling of amino acids in cell culture (SILAC) labeled SH-SY5Y cells left untreated or exposed to 400 nM okadaic acid for 50 min before mixed in a ratio 1:1 in 0.7% Triton X-100/MES buffer. After centrifugation in a sucrose gradient, 10 fractions of 0.5 mL were collected. The lipid raft fractions were identified by Western blotting using flotillin as a lipid raft marker. Fractions containing flotillin were pooled prior to removal of lipids by chloroform/methanol extraction, followed by acetone precipitation. Proteins were separated by SDS-PAGE. Proteins were in-gel digested with trypsin, and peptides were purified either with (1) C18 or (2) IMAC/TiO 2 and analyzed on LTQ-Orbitrap. Proteins were identified with Mascot (ver. 2.2) and quantified using MaxQuant (ver. 1.13).

    Journal: Marine Drugs

    Article Title: Identification of Dynamic Changes in Proteins Associated with the Cellular Cytoskeleton after Exposure to Okadaic Acid

    doi: 10.3390/md11061763

    Figure Lengend Snippet: Workflow to identify lipid raft-associated proteins. Stable isotopic labeling of amino acids in cell culture (SILAC) labeled SH-SY5Y cells left untreated or exposed to 400 nM okadaic acid for 50 min before mixed in a ratio 1:1 in 0.7% Triton X-100/MES buffer. After centrifugation in a sucrose gradient, 10 fractions of 0.5 mL were collected. The lipid raft fractions were identified by Western blotting using flotillin as a lipid raft marker. Fractions containing flotillin were pooled prior to removal of lipids by chloroform/methanol extraction, followed by acetone precipitation. Proteins were separated by SDS-PAGE. Proteins were in-gel digested with trypsin, and peptides were purified either with (1) C18 or (2) IMAC/TiO 2 and analyzed on LTQ-Orbitrap. Proteins were identified with Mascot (ver. 2.2) and quantified using MaxQuant (ver. 1.13).

    Article Snippet: For general lipid raft proteome mapping, the peptides were separated on a Dionex Ultimate 3000 nano-LC system (Sunnyvale, CA, USA) coupled to a linear quadrupole ion trap (LTQ-Orbitrap) mass spectrometer (Thermo Scientific).

    Techniques: Isotopic Labeling, Cell Culture, Labeling, Centrifugation, Western Blot, Marker, SDS Page, Purification

    Analysis of interaction dynamics by QUBIC triple SILAC based quantitative mass spectrometry. (A) Experimental workflow for triple SILAC pull-downs to determine Wnt3a-dependent interaction dynamics. The cell line expressing the GFP-tagged protein of interest is light and medium SILAC labeled, the untransfected wild-type control cell line is heavy SILAC labeled. Cells are lysed after two hour treatment with Wnt3a (200 ng/mL) or vehicle solution respectively. GFP-pull-downs are performed separately for each SILAC state. Eluates are combined, separated on a one-dimensional gel into eight slices and in-gel digested. Resulting peptide mixtures are analyzed by high resolution LC-MS/MS on an LTQ-Orbitrap Velos. SILAC ratios are automatically quantified by MaxQuant. (B) SILAC peptide triplets representing peak profiles characteristic of background, constitutive and dynamic binders. (C) Data analysis plot of the ratio representing interaction specificity versus the ratio representing stimulus specificity of the interaction. Filled dots represent significant interactors and of these, constitutive interactors are depicted in blue. Dynamic interactors with enhanced binding to the bait protein are shown in red and those with reduced binding to the bait protein in green.

    Journal: Journal of Proteome Research

    Article Title: Triple SILAC to Determine Stimulus Specific Interactions in the Wnt Pathway

    doi: 10.1021/pr200740a

    Figure Lengend Snippet: Analysis of interaction dynamics by QUBIC triple SILAC based quantitative mass spectrometry. (A) Experimental workflow for triple SILAC pull-downs to determine Wnt3a-dependent interaction dynamics. The cell line expressing the GFP-tagged protein of interest is light and medium SILAC labeled, the untransfected wild-type control cell line is heavy SILAC labeled. Cells are lysed after two hour treatment with Wnt3a (200 ng/mL) or vehicle solution respectively. GFP-pull-downs are performed separately for each SILAC state. Eluates are combined, separated on a one-dimensional gel into eight slices and in-gel digested. Resulting peptide mixtures are analyzed by high resolution LC-MS/MS on an LTQ-Orbitrap Velos. SILAC ratios are automatically quantified by MaxQuant. (B) SILAC peptide triplets representing peak profiles characteristic of background, constitutive and dynamic binders. (C) Data analysis plot of the ratio representing interaction specificity versus the ratio representing stimulus specificity of the interaction. Filled dots represent significant interactors and of these, constitutive interactors are depicted in blue. Dynamic interactors with enhanced binding to the bait protein are shown in red and those with reduced binding to the bait protein in green.

    Article Snippet: LC-MS/MS Analysis Eluted peptides were analyzed by a nanoflow HPLC (Proxeon Biosystems) coupled online via a nanoelectrospray ion source (Proxeon Biosystems) to a linear trap quadrupole (LTQ)-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).

    Techniques: Mass Spectrometry, Expressing, Labeling, Liquid Chromatography with Mass Spectroscopy, Binding Assay