lightcycler→ 480 sybr green i master mix  (Roche)

 
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    Roche lightcycler→ 480 sybr green i master mix
    Lightcycler→ 480 Sybr Green I Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler→ 480 sybr green i master mix/product/Roche
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lightcycler→ 480 sybr green i master mix - by Bioz Stars, 2020-01
    84/100 stars

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    SYBR Green Assay:

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Irisin gene expression was also assessed with a Taqman probe (fibronectin type III domain-containing protein 5 or FNDC5 , Hs00401006_m1). .. Uncoupling protein 1 (UCP1 ) and integrin subunit alpha 4 (ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ). .. Fw: 5′-CTCCAGGTCCAAGGTGAATG-3′ and Rv: 5′-TAGAGGCCGATCCTGAGAGA-3′ primers were used for UCP1 , while Fw: 5′-GCAAGGAAGTTCCAGGTTACA-3′, and Rv: 5′-CACGTCAGAAGTTCCATTAGAAGA-3′ primers were used for ITGA4 (Sigma-Aldrich, St. Louis, MO, United States).

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Irisin gene expression was also assessed with a Taqman probe (fibronectin type III domain-containing protein 5 or FNDC5 , Hs00401006_m1). .. Uncoupling protein 1 ( UCP1 ) and integrin subunit alpha 4 ( ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ). .. Fw: 5′-CTCCAGGTCCAAGGTGAATG-3′ and Rv: 5′-TAGAGGCCGATCCTGAGAGA-3′ primers were used for UCP1 , while Fw: 5′-GCAAGGAAGTTCCAGGTTACA-3′, and Rv: 5′-CACGTCAGAAGTTCCATTAGAAGA-3′ primers were used for ITGA4 (Sigma-Aldrich, St. Louis, MO, United States).

    Real-time Polymerase Chain Reaction:

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Gene expression levels of white, brown/beige and beige adipose tissue markers were quantified using Taqman probes in a QuantStudio® 7 Flex Real-Time PCR System (Applied Biosystems® ). .. Uncoupling protein 1 (UCP1 ) and integrin subunit alpha 4 (ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ).

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Gene expression levels of white, brown/beige and beige adipose tissue markers were quantified using Taqman probes in a QuantStudio® 7 Flex Real-Time PCR System (Applied Biosystems® ). .. Uncoupling protein 1 ( UCP1 ) and integrin subunit alpha 4 ( ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ).

    Expressing:

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Irisin gene expression was also assessed with a Taqman probe (fibronectin type III domain-containing protein 5 or FNDC5 , Hs00401006_m1). .. Uncoupling protein 1 (UCP1 ) and integrin subunit alpha 4 (ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ). .. Fw: 5′-CTCCAGGTCCAAGGTGAATG-3′ and Rv: 5′-TAGAGGCCGATCCTGAGAGA-3′ primers were used for UCP1 , while Fw: 5′-GCAAGGAAGTTCCAGGTTACA-3′, and Rv: 5′-CACGTCAGAAGTTCCATTAGAAGA-3′ primers were used for ITGA4 (Sigma-Aldrich, St. Louis, MO, United States).

    Article Title: Exercise Induces White Adipose Tissue Browning Across the Weight Spectrum in Humans
    Article Snippet: Irisin gene expression was also assessed with a Taqman probe (fibronectin type III domain-containing protein 5 or FNDC5 , Hs00401006_m1). .. Uncoupling protein 1 ( UCP1 ) and integrin subunit alpha 4 ( ITGA4 ) gene expression were assessed using the LightCycler→ 480 SYBR Green I Master mix (Roche) assay in a LightCycler→ 480 Instrument II (Roche® ). .. Fw: 5′-CTCCAGGTCCAAGGTGAATG-3′ and Rv: 5′-TAGAGGCCGATCCTGAGAGA-3′ primers were used for UCP1 , while Fw: 5′-GCAAGGAAGTTCCAGGTTACA-3′, and Rv: 5′-CACGTCAGAAGTTCCATTAGAAGA-3′ primers were used for ITGA4 (Sigma-Aldrich, St. Louis, MO, United States).

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    Roche lightcycler 480 sybr green i master mix
    Effect of the CASR rs7652589 G > A polymorphism on CaSR transcript levels in PBMC obtained from HD patients. The PBMC from whole venous blood were isolated by Ficoll-Hypaque centrifugation (density, 1.077 g/cm 3 ) followed by total RNA isolation. Quantitative analyses of CaSR transcript levels were performed by RT and Q-PCR <t>SYBR</t> Green I system. The quantity of CaSR transcript in each sample was standardized by the geometric mean of reference PBGD and B2M cDNA levels.
    Lightcycler 480 Sybr Green I Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master mix/product/Roche
    Average 99 stars, based on 359 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master mix - by Bioz Stars, 2020-01
    99/100 stars
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    Effect of the CASR rs7652589 G > A polymorphism on CaSR transcript levels in PBMC obtained from HD patients. The PBMC from whole venous blood were isolated by Ficoll-Hypaque centrifugation (density, 1.077 g/cm 3 ) followed by total RNA isolation. Quantitative analyses of CaSR transcript levels were performed by RT and Q-PCR SYBR Green I system. The quantity of CaSR transcript in each sample was standardized by the geometric mean of reference PBGD and B2M cDNA levels.

    Journal: Scientific Reports

    Article Title: Associations of the calcium-sensing receptor gene CASR rs7652589 SNP with nephrolithiasis and secondary hyperparathyroidism in haemodialysis patients

    doi: 10.1038/srep35188

    Figure Lengend Snippet: Effect of the CASR rs7652589 G > A polymorphism on CaSR transcript levels in PBMC obtained from HD patients. The PBMC from whole venous blood were isolated by Ficoll-Hypaque centrifugation (density, 1.077 g/cm 3 ) followed by total RNA isolation. Quantitative analyses of CaSR transcript levels were performed by RT and Q-PCR SYBR Green I system. The quantity of CaSR transcript in each sample was standardized by the geometric mean of reference PBGD and B2M cDNA levels.

    Article Snippet: For amplification, 1 μl of cDNA solution was added to 9 μl of LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics GmbH, Mannheim, Germany) and primers.

    Techniques: Isolation, Centrifugation, Polymerase Chain Reaction, SYBR Green Assay

    Effect of the rs6983267 SNP on MYC transcript levels in cancerous cervical tissues ( a ) and non-cancerous tissues ( b ). Frozen tissue was homogenized, followed by total RNA isolation. Quantitative analyses of MYC transcript levels were performed by qRT-PCR using the SYBR Green I system. The quantity of MYC transcript levels in each sample was standardized by the geometric mean of references using HMBS and B2M cDNA levels. Kruskal–Wallis test with a Dunn’s post hoc

    Journal: Archives of Gynecology and Obstetrics

    Article Title: The association of CCAT2 rs6983267 SNP with MYC expression and progression of uterine cervical cancer in the Polish population

    doi: 10.1007/s00404-018-4740-6

    Figure Lengend Snippet: Effect of the rs6983267 SNP on MYC transcript levels in cancerous cervical tissues ( a ) and non-cancerous tissues ( b ). Frozen tissue was homogenized, followed by total RNA isolation. Quantitative analyses of MYC transcript levels were performed by qRT-PCR using the SYBR Green I system. The quantity of MYC transcript levels in each sample was standardized by the geometric mean of references using HMBS and B2M cDNA levels. Kruskal–Wallis test with a Dunn’s post hoc

    Article Snippet: For amplification, 1 μl of cDNA solution was added to 9 μl of LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics GmbH, Mannheim, Germany) and primers (Supplementary data 1).

    Techniques: Isolation, Quantitative RT-PCR, SYBR Green Assay

    Effect of the GSDMB rs8067378 polymorphism on GSDMB1–4 isoform transcript levels in cancerous cervical tissues ( a ) and non-cancerous tissues ( b ). Frozen tissue was homogenized, followed by total RNA isolation. Quantitative analyses of GSDMB transcript levels were performed by quantitative real-time polymerase chain reaction using the SYBR Green I system. The quantity of GSDMB transcript levels in each sample was standardized by the geometric mean of references using hydroxymethylbilane synthase and β-2-microglobulin cDNA levels. Kruskal–Wallis test with a Dunn’s post-hoc or b Tukey’s post-hoc test. GSDMB gasdermin B

    Journal: Molecular Diagnosis & Therapy

    Article Title: Analysis of rs8067378 Polymorphism in the Risk of Uterine Cervical Cancer from a Polish Population and its Impact on Gasdermin B Expression

    doi: 10.1007/s40291-017-0256-1

    Figure Lengend Snippet: Effect of the GSDMB rs8067378 polymorphism on GSDMB1–4 isoform transcript levels in cancerous cervical tissues ( a ) and non-cancerous tissues ( b ). Frozen tissue was homogenized, followed by total RNA isolation. Quantitative analyses of GSDMB transcript levels were performed by quantitative real-time polymerase chain reaction using the SYBR Green I system. The quantity of GSDMB transcript levels in each sample was standardized by the geometric mean of references using hydroxymethylbilane synthase and β-2-microglobulin cDNA levels. Kruskal–Wallis test with a Dunn’s post-hoc or b Tukey’s post-hoc test. GSDMB gasdermin B

    Article Snippet: For amplification, 1 µL of cDNA solution was added to 9 µL of LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics GmbH, Mannheim, Germany) and primers (Online Resource 1).

    Techniques: Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay

    Principle of the SG-PERT assay. Cell-free retrovirus containing supernatant is lysed and added to a reaction mix containing the MS2 RNA template, MS2 complementary primers and a SYBR Green I qPCR mastermix. During a one-step reaction, the reverse transcriptase (RT) enzymes derived from the retroviral particles will convert the MS2 RNA into cDNA and cDNA is subsequently quantified by qPCR amplification of the MS2 cDNA. The amount of synthesized cDNA represents the level of RT activity in the viral supernatant and is thereby a measure of the amount of retroviral particles.

    Journal: PLoS ONE

    Article Title: Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

    doi: 10.1371/journal.pone.0050859

    Figure Lengend Snippet: Principle of the SG-PERT assay. Cell-free retrovirus containing supernatant is lysed and added to a reaction mix containing the MS2 RNA template, MS2 complementary primers and a SYBR Green I qPCR mastermix. During a one-step reaction, the reverse transcriptase (RT) enzymes derived from the retroviral particles will convert the MS2 RNA into cDNA and cDNA is subsequently quantified by qPCR amplification of the MS2 cDNA. The amount of synthesized cDNA represents the level of RT activity in the viral supernatant and is thereby a measure of the amount of retroviral particles.

    Article Snippet: For SG-PERT on the LightCycler® 480 (Roche Diagnostics), the LightCycler® 480 SYBR Green I Master mix from Roche was used as reaction mix (Catalog #04707516001).

    Techniques: SYBR Green Assay, Real-time Polymerase Chain Reaction, Derivative Assay, Amplification, Synthesized, Activity Assay