lightcycler 480 sybr green i master mix kit  (Roche)


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    Roche lightcycler 480 sybr green i master mix kit
    Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of <t>SYBR</t> <t>Green</t> I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.
    Lightcycler 480 Sybr Green I Master Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master mix kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master mix kit - by Bioz Stars, 2021-09
    86/100 stars

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    1) Product Images from "The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability"

    Article Title: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

    Journal: Epigenetics & Chromatin

    doi: 10.1186/1756-8935-5-5

    Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of SYBR Green I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.
    Figure Legend Snippet: Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of SYBR Green I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.

    Techniques Used: Concentration Assay, SYBR Green Assay, Staining, Quantitation Assay

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    Article Snippet: .. Quantitative analysis of expression of T-bet, GATA3, RORγt and FOXP3 genes was performed using LightCycler® 480 System (Roche) in 384-well optical plates with Maxima SYBR Green qPCR Master Mix (Thermofisher Scientific) according to the manufacturer’s instructions. ..

    SYBR Green Assay:

    Article Title: Estrogen receptor α/HDAC/NFAT axis for delphinidin effects on proliferation and differentiation of T lymphocytes from patients with cardiovascular risks
    Article Snippet: .. Quantitative analysis of expression of T-bet, GATA3, RORγt and FOXP3 genes was performed using LightCycler® 480 System (Roche) in 384-well optical plates with Maxima SYBR Green qPCR Master Mix (Thermofisher Scientific) according to the manufacturer’s instructions. ..

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    Article Title: A novel lineage of candidate pheromone receptors for sex communication in moths
    Article Snippet: .. Gene-specific primers were designed for SlitOr5 (Or5up : 5’-TCGGGAGAAACTGAAGGACGTTGT-3’, Or5do : 5’-GCACGGAACCGCACTTATCACTAT-3’) and for the reference gene SlitRpl13 (Rpl13up : 5’-GTACCTGCCGCTCTCCGTGT-3’, Rpl13do : 5’-CTGCGGTGAATGGTGCTGTC-3’). qPCR mix was prepared in a total volume of 10 μL with 5 μL of LightCycler® 480 SYBR Green I Master (Roche, Basel, Switzerland), 4 μL of diluted cDNA (or water for the negative control) and 0.5 μM of each primer. qPCR assays were performed using the LightCycler® 480 Real-Time PCR system (Roche). ..

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    Article Title: Characterisation of peripheral and central components of the rat monoiodoacetate model of Osteoarthritis
    Article Snippet: .. First strand cDNA synthesis was performed on 500 ng RNA using a Superscript III Reverse Transcriptase kit (Invitrogen) according to manufacturers instructions with deoxynucleotide-triphosphates (Promega), and random primers (Promega). mRNA levels of IL-1β, TNFα, IL-6, ATF-3, Iba1, and glial fibrillary acid protein (GFAP) were measured with quantitative PCR using specific primers ( ) and LightCycler® 480 SYBR Green I master mix (Roche). ..

    Article Title: Production and Functional Characterization of Murine Osteoclasts Differentiated from ER-Hoxb8-Immortalized Myeloid Progenitor Cells
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    Real-time Polymerase Chain Reaction:

    Article Title: Estrogen receptor α/HDAC/NFAT axis for delphinidin effects on proliferation and differentiation of T lymphocytes from patients with cardiovascular risks
    Article Snippet: .. Quantitative analysis of expression of T-bet, GATA3, RORγt and FOXP3 genes was performed using LightCycler® 480 System (Roche) in 384-well optical plates with Maxima SYBR Green qPCR Master Mix (Thermofisher Scientific) according to the manufacturer’s instructions. ..

    Article Title: The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis
    Article Snippet: .. Quantification was performed with gene-specific primers (see Supplemental Material Table ), which were designed by the Primer-Blast tool and based on the genome sequence for Mtb H37Rv with Gene bank access number NC_000962.3. qPCR was performed by using the start SYBR Green I Master mix and the LightCycler® 480 system (Life Science Roche). ..

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    Sequencing:

    Article Title: The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis
    Article Snippet: .. Quantification was performed with gene-specific primers (see Supplemental Material Table ), which were designed by the Primer-Blast tool and based on the genome sequence for Mtb H37Rv with Gene bank access number NC_000962.3. qPCR was performed by using the start SYBR Green I Master mix and the LightCycler® 480 system (Life Science Roche). ..

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    Negative Control:

    Article Title: A novel lineage of candidate pheromone receptors for sex communication in moths
    Article Snippet: .. Gene-specific primers were designed for SlitOr5 (Or5up : 5’-TCGGGAGAAACTGAAGGACGTTGT-3’, Or5do : 5’-GCACGGAACCGCACTTATCACTAT-3’) and for the reference gene SlitRpl13 (Rpl13up : 5’-GTACCTGCCGCTCTCCGTGT-3’, Rpl13do : 5’-CTGCGGTGAATGGTGCTGTC-3’). qPCR mix was prepared in a total volume of 10 μL with 5 μL of LightCycler® 480 SYBR Green I Master (Roche, Basel, Switzerland), 4 μL of diluted cDNA (or water for the negative control) and 0.5 μM of each primer. qPCR assays were performed using the LightCycler® 480 Real-Time PCR system (Roche). ..

    Polymerase Chain Reaction:

    Article Title: Chemokine expression in renal ischemia/reperfusion injury is most profound during the reparative phase
    Article Snippet: .. Quantitative real-time reverse transcription PCR (QRT–PCR) was performed on a LightCycler® 480 System (Roche) using LightCycler® 480 SYBR Green I Master mix (Roche). ..

    Quantitative RT-PCR:

    Article Title: Chemokine expression in renal ischemia/reperfusion injury is most profound during the reparative phase
    Article Snippet: .. Quantitative real-time reverse transcription PCR (QRT–PCR) was performed on a LightCycler® 480 System (Roche) using LightCycler® 480 SYBR Green I Master mix (Roche). ..

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    Isolation:

    Article Title: Production and Functional Characterization of Murine Osteoclasts Differentiated from ER-Hoxb8-Immortalized Myeloid Progenitor Cells
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  • 86
    Roche lightcycler 480 sybr green i master mix kit
    Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of <t>SYBR</t> <t>Green</t> I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.
    Lightcycler 480 Sybr Green I Master Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master mix kit/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master mix kit - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Roche lightcycler 480 sybr green i master kit mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Kit Mix, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master kit mix/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybr green i master kit mix - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of SYBR Green I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.

    Journal: Epigenetics & Chromatin

    Article Title: The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability

    doi: 10.1186/1756-8935-5-5

    Figure Lengend Snippet: Lack of the H4 C-terminal tail but not H4 G94P alters anti-silencing function 1 (Asf1)-mediated disome formation. H3/H4 G94P (A) or H3/H4 ∆94 (B) were compared to wild-type (WT) H3/H4, all at 0.8 μM dimer concentration, for their ability to form tetrasomes and disomes on 80 bp 5 S DNA (0.4 μM) in the absence and presence of Asf1 (0, 0.8, 2.0 μM). Upper panels show images of SYBR Green I stained DNA, and lower panels show the quantitation of the amount of disomes and tetrasomes formed for each type of histone, from at least three independent experiments. Tetrasome and disome levels were normalized to the WT H3/H4 sample in the absence of Asf1.

    Article Snippet: ChIP quantification was performed via real-time PCR with a Roche Applied Sciences LightCycler 480 and the LightCycler 480 SYBR Green I Master Mix kit (Roche catalog no. 04707516001).

    Techniques: Concentration Assay, SYBR Green Assay, Staining, Quantitation Assay

    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction, SYBR Green Assay

    NRF-1, KLF15, and Sp1 expression in HepG2 and HT1080 cells and the impact of NRF-1, KLF15 and Sp1 on hPCFT gene expression. ( A ) Transcript levels for KLF15, NRF-1 and Sp1 were measured in HepG2 and HT1080 cell lines. ( B ) Impact of hPCFT gene expression in HepG2 cells stably transfected with KLF15, NRF-1 or Sp1. ( C ) Impact of hPCFT gene expression in HT1080 cells stably transfected with KLF15, NRF-1 or Sp1. ( D ) Impact of hPCFT gene expression in HT1080 cells transiently transfected with KLF15, NRF-1 or Sp1 singly or in combination. Transcript levels of NRF-1, KLF15, Sp1 and hPCFT were monitored by real-time RT-PCR with a LightCycler 480 Probes Master kit, or with a LightCycler 480 SYBR Green I Master kit. The transcript levels were normalized to that for GAPDH and/or β-actin. Results are presented as mean values ± standard errors from three to four different experiments. For statistics: *** p

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: NRF-1, KLF15, and Sp1 expression in HepG2 and HT1080 cells and the impact of NRF-1, KLF15 and Sp1 on hPCFT gene expression. ( A ) Transcript levels for KLF15, NRF-1 and Sp1 were measured in HepG2 and HT1080 cell lines. ( B ) Impact of hPCFT gene expression in HepG2 cells stably transfected with KLF15, NRF-1 or Sp1. ( C ) Impact of hPCFT gene expression in HT1080 cells stably transfected with KLF15, NRF-1 or Sp1. ( D ) Impact of hPCFT gene expression in HT1080 cells transiently transfected with KLF15, NRF-1 or Sp1 singly or in combination. Transcript levels of NRF-1, KLF15, Sp1 and hPCFT were monitored by real-time RT-PCR with a LightCycler 480 Probes Master kit, or with a LightCycler 480 SYBR Green I Master kit. The transcript levels were normalized to that for GAPDH and/or β-actin. Results are presented as mean values ± standard errors from three to four different experiments. For statistics: *** p

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Expressing, Stable Transfection, Transfection, Quantitative RT-PCR, SYBR Green Assay

    Expression and regulatory associations between hPCFT and NRF-1, Sp1, or KLF15 in solid tumor cell lines. ) by real-time RT-PCR with LightCycler 480 SYBR Green I Master kit. The hPCFT, NRF-1, Sp1, and KLF15 transcript levels were normalized to transcript levels for GAPDH. The scatter plots show univariate associations between hPCFT and NRF-1 ( A ), hPCFT and Sp1 ( B ), and hPCFT and KLF15 ( C ) by Pearson’s correlation coefficients ( r ). The x-axis and y-axis scales are logarithmic and the solid gray lines represent the fitted regression lines. N indicates the sample size (53). ( D ]. For the schematic shown, the thickness of the edge and the corresponding numbers (which range from 0 to 1) represent the relative strength of the association. The R/Bioconductor packages minet and igraph ].

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: Expression and regulatory associations between hPCFT and NRF-1, Sp1, or KLF15 in solid tumor cell lines. ) by real-time RT-PCR with LightCycler 480 SYBR Green I Master kit. The hPCFT, NRF-1, Sp1, and KLF15 transcript levels were normalized to transcript levels for GAPDH. The scatter plots show univariate associations between hPCFT and NRF-1 ( A ), hPCFT and Sp1 ( B ), and hPCFT and KLF15 ( C ) by Pearson’s correlation coefficients ( r ). The x-axis and y-axis scales are logarithmic and the solid gray lines represent the fitted regression lines. N indicates the sample size (53). ( D ]. For the schematic shown, the thickness of the edge and the corresponding numbers (which range from 0 to 1) represent the relative strength of the association. The R/Bioconductor packages minet and igraph ].

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay

    Transcriptional regulation of hPCFT gene expression. ( A ) R1–11 HeLa, HT1080 and HepG2 cells were plated and treated with 2 μM 5-Aza or DMSO (vehicle) for 72 h. The cells were harvested for RNA extraction for hPCFT gene expression analysis by real-time RT-PCR with a LightCycler 480 Probes Master kit. Results are shown as mean values ± standard errors ( n = 3). * p

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: Transcriptional regulation of hPCFT gene expression. ( A ) R1–11 HeLa, HT1080 and HepG2 cells were plated and treated with 2 μM 5-Aza or DMSO (vehicle) for 72 h. The cells were harvested for RNA extraction for hPCFT gene expression analysis by real-time RT-PCR with a LightCycler 480 Probes Master kit. Results are shown as mean values ± standard errors ( n = 3). * p

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Expressing, RNA Extraction, Quantitative RT-PCR