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Roche lightcycler 480 qrt pcr system
Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean <t>qRT-PCR</t> data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p
Lightcycler 480 Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lightcycler 480 qrt pcr system/product/Roche
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
lightcycler 480 qrt pcr system - by Bioz Stars, 2020-02
99/100 stars

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1) Product Images from "Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire"

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.RA118.005992

Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p
Figure Legend Snippet: Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

Techniques Used: Activity Assay, In Vivo, Expressing, Quantitative RT-PCR

2) Product Images from "Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire"

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.RA118.005992

Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p
Figure Legend Snippet: Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

Techniques Used: Activity Assay, In Vivo, Expressing, Quantitative RT-PCR

3) Product Images from "Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris"

Article Title: Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2016.00574

qRT-PCR analysis of tissue-specific relative expression levels of locust insulin-related peptide-like 1 ( LIRP ) across castes of B. terrestris . LIRP is part of the insulin signaling involved in reproduction and diapause. It is most abundant in fat body and ventriculus of males and reproducing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of locust insulin-related peptide-like 1 ( LIRP ) across castes of B. terrestris . LIRP is part of the insulin signaling involved in reproduction and diapause. It is most abundant in fat body and ventriculus of males and reproducing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of forkhead box protein O ( FOXO ) across castes of B. terrestris . FOXO is a transcription factor involved in stress tolerance, diapause, longevity, and growth. FOXO transcription is lower in workers and reproducing queens than in other castes (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of forkhead box protein O ( FOXO ) across castes of B. terrestris . FOXO is a transcription factor involved in stress tolerance, diapause, longevity, and growth. FOXO transcription is lower in workers and reproducing queens than in other castes (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of insulin-like peptide receptor 2 ( InR-2 ) across castes of B. terrestris . InR-2 is a receptor involved in insulin signaling. It is upregulated in gonads (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of insulin-like peptide receptor 2 ( InR-2 ) across castes of B. terrestris . InR-2 is a receptor involved in insulin signaling. It is upregulated in gonads (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of adipokinetic hormone ( AKH ) across castes of B. terrestris . AKH is involved in the mobilization of lipid, carbohydrates and/or proline stores. AKH is mainly expressed in brain-CC-CA samples, but minor expression occurred also in other tissues (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of adipokinetic hormone ( AKH ) across castes of B. terrestris . AKH is involved in the mobilization of lipid, carbohydrates and/or proline stores. AKH is mainly expressed in brain-CC-CA samples, but minor expression occurred also in other tissues (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of Krüppel homolog 1 ( Kr-h1 ) across castes of B. terrestris . Kr-h1 is a transcription factor downstream of juvenile hormone receptors. It is downregulated in virgin queens, and upregulated in males and diapausing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of Krüppel homolog 1 ( Kr-h1 ) across castes of B. terrestris . Kr-h1 is a transcription factor downstream of juvenile hormone receptors. It is downregulated in virgin queens, and upregulated in males and diapausing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of vitellogenin ( Vg ) across castes of B. terrestris . Vg is a yolk protein with multiple functions in immunity and behavioral regulation. It is highly upregulated in fat body of all castes, and generally lowest in males and diapausing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of vitellogenin ( Vg ) across castes of B. terrestris . Vg is a yolk protein with multiple functions in immunity and behavioral regulation. It is highly upregulated in fat body of all castes, and generally lowest in males and diapausing queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of insulin-like growth factor 1 ( IGF-1 ) across castes of B. terrestris . IGF-1 is part of the IIS signaling involved in reproduction and diapause. It is queen-specific (i.e., absent in workers and males) and lowest in virgin queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in (B) linear scale; and (C) log-transformed scale. Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of insulin-like growth factor 1 ( IGF-1 ) across castes of B. terrestris . IGF-1 is part of the IIS signaling involved in reproduction and diapause. It is queen-specific (i.e., absent in workers and males) and lowest in virgin queens (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in (B) linear scale; and (C) log-transformed scale. Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of insulin-like peptide receptor 1 ( InR-1 ) across castes of B. terrestris . InR-1 is a receptor involved in insulin signaling. It is upregulated in virgin and diapausing queens, especially in crop and hypopharyngeal glands (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of insulin-like peptide receptor 1 ( InR-1 ) across castes of B. terrestris . InR-1 is a receptor involved in insulin signaling. It is upregulated in virgin and diapausing queens, especially in crop and hypopharyngeal glands (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of methyl farnesoate epoxidase ( MFE ) across castes of B. terrestris . MFE is crucial in synthetizing juvenile hormone (JH). It is heavily upregulated in gonads of workers and reproducing queen. It is also found in brains of all samples (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of methyl farnesoate epoxidase ( MFE ) across castes of B. terrestris . MFE is crucial in synthetizing juvenile hormone (JH). It is heavily upregulated in gonads of workers and reproducing queen. It is also found in brains of all samples (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: Relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

qRT-PCR analysis of tissue-specific relative expression levels of adipokinetic hormone receptor ( AKHR ) across castes of B. terrestris . AKHR is involved in the regulation of energy storage. It is mainly expressed in fat body and hypopharyngeal glands (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p
Figure Legend Snippet: qRT-PCR analysis of tissue-specific relative expression levels of adipokinetic hormone receptor ( AKHR ) across castes of B. terrestris . AKHR is involved in the regulation of energy storage. It is mainly expressed in fat body and hypopharyngeal glands (see main text). Data represent mean ± SEM ( n = 3–5) of normalized and rescaled expression levels. (A) Heatmap: Log-scale. Bar graphs: relative transcript levels in linear scale (B) and log-transformed scale (C) . Significantly different expression levels are indicated by different letters ( p

Techniques Used: Quantitative RT-PCR, Expressing, Transformation Assay

Related Articles

In Vivo:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: Paragraph title: Induction of Kr-h1 mRNA expression in vivo ... Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ).

Amplification:

Article Title: Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris
Article Snippet: qRT-PCR analysis We performed qRT-PCR using a LightCycler 480 qRT-PCR System (Roche) with SYBR green fluorescent labels and 200 nM of each primer. .. The final extension was at 72°C for 2 min. A final melt-curve step was included post-PCR (ramping from 55°C to 95°C at 0.1°C steps every 5 s) to confirm the absence of any non-specific amplification.

RNA Extraction:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: The puparia were immediately frozen and subjected to total RNA extraction using TRI Reagent (Sigma-Aldrich) following the manufacturer's protocol. .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ).

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: The puparia were immediately frozen and subjected to total RNA extraction using TRI Reagent (Sigma-Aldrich) following the manufacturer's protocol. .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ).

Quantitative RT-PCR:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ). .. To enable comparisons among all samples, a calibrator cDNA was applied to a master mix for specific genes on each plate, and Kr-h1 expression was normalized relative to levels of rp49 .

Article Title: Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris
Article Snippet: .. qRT-PCR analysis We performed qRT-PCR using a LightCycler 480 qRT-PCR System (Roche) with SYBR green fluorescent labels and 200 nM of each primer. .. The PCR program was as follows: Initial denaturation for 15 min at 95°C, followed by 40 cycles of 30 s at 95°C, 30 s at 55°C and 30 s at 72°C.

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ). .. To enable comparisons among all samples, a calibrator cDNA was applied to a master mix for specific genes on each plate, and Kr-h1 expression was normalized relative to levels of rp49 .

SYBR Green Assay:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ). .. To enable comparisons among all samples, a calibrator cDNA was applied to a master mix for specific genes on each plate, and Kr-h1 expression was normalized relative to levels of rp49 .

Article Title: Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris
Article Snippet: .. qRT-PCR analysis We performed qRT-PCR using a LightCycler 480 qRT-PCR System (Roche) with SYBR green fluorescent labels and 200 nM of each primer. .. The PCR program was as follows: Initial denaturation for 15 min at 95°C, followed by 40 cycles of 30 s at 95°C, 30 s at 55°C and 30 s at 72°C.

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ). .. To enable comparisons among all samples, a calibrator cDNA was applied to a master mix for specific genes on each plate, and Kr-h1 expression was normalized relative to levels of rp49 .

Random Hexamer Labeling:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: RNA was treated with TURBO RNase-free DNase (Ambion), and 2-μg RNA aliquots were used for first-strand cDNA synthesis using the SuperScript III kit (Invitrogen) with random hexamer primers. .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ).

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: RNA was treated with TURBO RNase-free DNase (Ambion), and 2-μg RNA aliquots were used for first-strand cDNA synthesis using the SuperScript III kit (Invitrogen) with random hexamer primers. .. Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ).

Expressing:

Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire
Article Snippet: Paragraph title: Induction of Kr-h1 mRNA expression in vivo ... Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 (rp49 ) genes ( ).

Polymerase Chain Reaction:

Article Title: Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris
Article Snippet: qRT-PCR analysis We performed qRT-PCR using a LightCycler 480 qRT-PCR System (Roche) with SYBR green fluorescent labels and 200 nM of each primer. .. The PCR program was as follows: Initial denaturation for 15 min at 95°C, followed by 40 cycles of 30 s at 95°C, 30 s at 55°C and 30 s at 72°C.

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  • 99
    Roche lightcycler 480 real time qrt pcr instrument
    ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue <t>qRT-PCR</t> array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p
    Lightcycler 480 Real Time Qrt Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 real time qrt pcr instrument/product/Roche
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 real time qrt pcr instrument - by Bioz Stars, 2020-02
    99/100 stars
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    79
    Roche sybr green quantitative real time polymerase chain reaction qrt pcr
    Expression of NF-κB1 and Bcl-2 detection in glioma tissues by <t>qRT-PCR.</t> Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P
    Sybr Green Quantitative Real Time Polymerase Chain Reaction Qrt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green quantitative real time polymerase chain reaction qrt pcr/product/Roche
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    sybr green quantitative real time polymerase chain reaction qrt pcr - by Bioz Stars, 2020-02
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    99
    Roche lightcycler 480 qrt pcr system
    Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean <t>qRT-PCR</t> data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p
    Lightcycler 480 Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 qrt pcr system/product/Roche
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 qrt pcr system - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    97
    Roche qrt pcr analysis
    Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by <t>qRT-PCR.</t> The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p
    Qrt Pcr Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr analysis/product/Roche
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    ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue qRT-PCR array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p

    Journal: Frontiers in Immunology

    Article Title: Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia

    doi: 10.3389/fimmu.2018.01661

    Figure Lengend Snippet: ZNF554 down-regulation in the villous trophoblast (VT) in preeclampsia. (A) Tissue qRT-PCR array revealed the highest ZNF554 expression in the placenta among 48 human tissues. Color code depicts gene expression levels relative to that of the placenta (100%). (B) In situ hybridization of a third trimester control placenta (GW29) and (C) immunohistochemistry of a first trimester placenta (GW12) shows mainly syncytiotrophoblastic ZNF554 expression (hematoxylin counterstaining, 1,400 and 400× magnifications, respectively). Black or white arrowheads depict syncytiotrophoblast or cytotrophoblast, while black arrow depicts fetal endothelium, respectively. (D) qRT-PCR revealed that ZNF554 expression is up-regulated during VT differentiation in parallel with CSH1 . (E,F) ZNF554 immunopositivity was faint in the syncytiotrophoblast in preeclampsia [ (F) , GW35] compared to gestational-age matched controls [ (E) , GW36] (hematoxylin counterstaining, 400× magnifications). Arrow and arrowhead depict syncytiotrophoblast and villous endothelium, respectively. (G) ZNF554 mRNA expression was 74% lower in ZNF554 -silenced BeWo cells compared to controls used for the microarrays ( p = 5.24 × 10 −6 ). (H) Nuclear and cytoplasmic ZNF554 immunofluorescence decreased in BeWo cells treated with ZNF554 siRNA compared to control cells (3,500× magnifications). (I) Molecular functions and one Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (glycolysis/gluconeogenesis) affected in ZNF554 -silenced BeWo cells. Colors denote the proportions of up- or down-regulated genes (red: > 0.5 up-regulated; blue: > 0.5 down-regulated; black: 0.5–0.5 up- and down-regulated). Letter sizes represent the minus log 10 of p -values of the given functions or pathway. (J) qRT-PCR validated FSTL3 up-regulation (2.7-fold, p

    Article Snippet: Quality control criteria included robust and specific amplification (Cp values < 40 cycles and CV < 10% for duplicates) of the bisulfite primers on a LightCycler 480 real-time qRT-PCR instrument (Roche Diagnostics Corp. Indianapolis, IN, USA).

    Techniques: Quantitative RT-PCR, Expressing, In Situ Hybridization, Immunohistochemistry, Immunofluorescence

    Gene modules associated with blood pressure (BP) and birthweight (BW). (A) Hierarchical clustering of qRT-PCR data obtained with 100 samples and 47 genes. Pearson correlation was used for similarity analysis and average method for linkage calculation. Samples were colored according to patient groups and maturity status. M1 (green) and M2 (red) module genes and 34 of 60 samples from women with preeclampsia clustered together. (B) Association of gene expression with BP and BW. The significance p -values for these coefficients were plotted for all genes, colored according to module classification (black: not changed on the microarray). Filled circles represent predominantly placenta-expressed genes and dashed lines the significance threshold at p = 0.05. Seven of 9 genes related to BW belong to module M1, while 10 of 15 genes related to BP are from module M2. Gene expression (C) and protein immunostaining (D) in Supplementary Material. (E) Representative images of the same placenta from a preterm control (left, 29 weeks) and a patient with preterm preeclampsia associated with SGA (right, 31 weeks) are shown for the immunostainings (hematoxylin counterstaining, 40× magnification).

    Journal: Frontiers in Immunology

    Article Title: Integrated Systems Biology Approach Identifies Novel Maternal and Placental Pathways of Preeclampsia

    doi: 10.3389/fimmu.2018.01661

    Figure Lengend Snippet: Gene modules associated with blood pressure (BP) and birthweight (BW). (A) Hierarchical clustering of qRT-PCR data obtained with 100 samples and 47 genes. Pearson correlation was used for similarity analysis and average method for linkage calculation. Samples were colored according to patient groups and maturity status. M1 (green) and M2 (red) module genes and 34 of 60 samples from women with preeclampsia clustered together. (B) Association of gene expression with BP and BW. The significance p -values for these coefficients were plotted for all genes, colored according to module classification (black: not changed on the microarray). Filled circles represent predominantly placenta-expressed genes and dashed lines the significance threshold at p = 0.05. Seven of 9 genes related to BW belong to module M1, while 10 of 15 genes related to BP are from module M2. Gene expression (C) and protein immunostaining (D) in Supplementary Material. (E) Representative images of the same placenta from a preterm control (left, 29 weeks) and a patient with preterm preeclampsia associated with SGA (right, 31 weeks) are shown for the immunostainings (hematoxylin counterstaining, 40× magnification).

    Article Snippet: Quality control criteria included robust and specific amplification (Cp values < 40 cycles and CV < 10% for duplicates) of the bisulfite primers on a LightCycler 480 real-time qRT-PCR instrument (Roche Diagnostics Corp. Indianapolis, IN, USA).

    Techniques: Quantitative RT-PCR, Expressing, Microarray, Immunostaining

    Expression of NF-κB1 and Bcl-2 detection in glioma tissues by qRT-PCR. Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P

    Journal: OncoTargets and therapy

    Article Title: Nuclear factor-kappa B1 inhibits early apoptosis of glioma cells by promoting the expression of Bcl-2

    doi: 10.2147/OTT.S144014

    Figure Lengend Snippet: Expression of NF-κB1 and Bcl-2 detection in glioma tissues by qRT-PCR. Notes: The expression of NF-κB1 in glioma was significantly higher than that in nonneoplastic brain tissues ( P

    Article Snippet: Relative levels of NF-κB1 and Bcl-2 mRNA were examined using SYBR green quantitative real-time polymerase chain reaction (qRT-PCR) (LightCycler® 480; Hoffman-La Roche Ltd., Basel, Switzerland) and were normalized to levels of GAPDH mRNA.

    Techniques: Expressing, Quantitative RT-PCR

    Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

    Journal: The Journal of Biological Chemistry

    Article Title: Exquisite ligand stereoselectivity of a Drosophila juvenile hormone receptor contrasts with its broad agonist repertoire

    doi: 10.1074/jbc.RA118.005992

    Figure Lengend Snippet: Activity of the JH I geometric isomers in vivo . A , capacity of the native S -( E , E )-JH I, its stereoisomers, and deoxy-JH I to induce ectopic expression of Kr-h1 mRNA in Drosophila pupae. Animals were treated at the white puparium stage and collected 24 h later. Shown are normalized mean qRT-PCR data from four biological replicates, each comprising three individual pupae for each compound. The mRNA levels are plotted on a logarithmic scale as -fold increase relative to treatment with solvent (acetone) alone for which the value was arbitrarily set to 1. Error bars represent S.D. Different letters above the data indicate that activity of the individual compounds differed significantly ( p

    Article Snippet: Transcripts were quantified using a LightCycler 480 qRT-PCR System (Roche Applied Science) with SYBR Green fluorescent label and previously described primers specific for Kr-h1 and the ribosomal protein 49 ( rp49 ) genes ( ).

    Techniques: Activity Assay, In Vivo, Expressing, Quantitative RT-PCR

    Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p

    Journal: Cells

    Article Title: Carnosine Prevents Aβ-Induced Oxidative Stress and Inflammation in Microglial Cells: A Key Role of TGF-β1

    doi: 10.3390/cells8010064

    Figure Lengend Snippet: Carnosine suppresses the Aβ1-42-induced mRNA expression levels of iNOS, Nox1, and Nox2 and increases the expression of TGF-β1 mRNA. Effects of Aβ1-42 and carnosine (Aβ1-42 + Car (co-treat.) and Aβ1-42 + Car (co-inc.)) on ( A ) iNOS, ( B ) Nox1, ( C ) Nox2, and ( D ) TGF-β1 mRNAs expression were examined by qRT-PCR. The abundance of each mRNA of interest was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in the absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed in quadruplicate. Standard deviations are represented by vertical bars. * significantly different from resting cells, p

    Article Snippet: Next, each sample was quantified, diluted to a final concentration of 25 ng/µL, and used for qRT-PCR analysis (LightCycler® 480 System, Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Negative Control