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Roche light cycle 480 pcr instrument
Light Cycle 480 Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/light cycle 480 pcr instrument/product/Roche
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
light cycle 480 pcr instrument - by Bioz Stars, 2020-01
79/100 stars

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High Throughput Screening Assay:

Article Title: Long-read ChIA-PET for base-pair resolution mapping of haplotype-specific chromatin interactions
Article Snippet: .. Agilent 2100 bioanalyzer instrument (Agilent techcologies, cat. no. G2940CA) Bio-Rad C1000 Thermal Cycler (Bio-rad, cat. no. 185-1148EDU) Biological safety cabinet classII, type A2 (Nuair, model no. NU-S480-600) Blue pippin instrument (Sage science, Blue Pippin) Centrifuge (Eppendorf 5810R, cat. no. 22628180) DynaMag™-2 Magnet (Life Technologies, cat. no. 12321D) Dark Reader Transilluminator (Clare Chemical Research, Inc. Model: DR89X) Eppendorf 5424 Ventilated Microcentrifuge (Eppendorf, cat. no. 022620401) Eppendorf Thermomixer (Eppendorf, cat. no. 5382000023) Freezer (Fisher Scientific, cat. no. 3752-DB) Illumina MiSeq and HiSeq2500 or NextSeq500 high-throughput sequencing machine (Illumina) Incubators (Heratherm, cat. no. 50125590) Intelli-mixer RM-2L (Rose scientific, cat. no. MX1000) Light cycle 480 PCR instrument (Roche, cat. no. 05015243001) MicroVac Concentrator (Tomy, cat. no. MV-100) Nikon TS100-F Microscope (Nikon, cat. no. TS100-F) Qubit 2.0 Fluorometer Specifications (Invitrogen, cat. no. ) Refrigerator (Fisher Scientific, cat. no. 13-994-201) Sonicator (SONICS & MATERIALS, cat. no. VCX130) Vortex-Genie® Mixers (VWR, cat. no. 58816-121) Water bath (Fisher Scientific, cat. no. 15-462-20Q) .. Cutadapt package (version 1.6) ( ) BWA software package (version 0.7.12-r1039 or above) ( ) SAMtools (version 0.1.19 and above) ( ) Picard tools (version 1.107 and above) ( ) BEDTools (version 2.17.0 and above) ( ) pysam python module (version 0.8.1) ( ) Perl scripts prepare_reads_single.pl (Supplementary Software 1) and prepare_reads_pair.pl (Supplementary Software 2) for bridge-linker trimming.

Sequencing:

Article Title: Long-read ChIA-PET for base-pair resolution mapping of haplotype-specific chromatin interactions
Article Snippet: .. Agilent 2100 bioanalyzer instrument (Agilent techcologies, cat. no. G2940CA) Bio-Rad C1000 Thermal Cycler (Bio-rad, cat. no. 185-1148EDU) Biological safety cabinet classII, type A2 (Nuair, model no. NU-S480-600) Blue pippin instrument (Sage science, Blue Pippin) Centrifuge (Eppendorf 5810R, cat. no. 22628180) DynaMag™-2 Magnet (Life Technologies, cat. no. 12321D) Dark Reader Transilluminator (Clare Chemical Research, Inc. Model: DR89X) Eppendorf 5424 Ventilated Microcentrifuge (Eppendorf, cat. no. 022620401) Eppendorf Thermomixer (Eppendorf, cat. no. 5382000023) Freezer (Fisher Scientific, cat. no. 3752-DB) Illumina MiSeq and HiSeq2500 or NextSeq500 high-throughput sequencing machine (Illumina) Incubators (Heratherm, cat. no. 50125590) Intelli-mixer RM-2L (Rose scientific, cat. no. MX1000) Light cycle 480 PCR instrument (Roche, cat. no. 05015243001) MicroVac Concentrator (Tomy, cat. no. MV-100) Nikon TS100-F Microscope (Nikon, cat. no. TS100-F) Qubit 2.0 Fluorometer Specifications (Invitrogen, cat. no. ) Refrigerator (Fisher Scientific, cat. no. 13-994-201) Sonicator (SONICS & MATERIALS, cat. no. VCX130) Vortex-Genie® Mixers (VWR, cat. no. 58816-121) Water bath (Fisher Scientific, cat. no. 15-462-20Q) .. Cutadapt package (version 1.6) ( ) BWA software package (version 0.7.12-r1039 or above) ( ) SAMtools (version 0.1.19 and above) ( ) Picard tools (version 1.107 and above) ( ) BEDTools (version 2.17.0 and above) ( ) pysam python module (version 0.8.1) ( ) Perl scripts prepare_reads_single.pl (Supplementary Software 1) and prepare_reads_pair.pl (Supplementary Software 2) for bridge-linker trimming.

Microscopy:

Article Title: Long-read ChIA-PET for base-pair resolution mapping of haplotype-specific chromatin interactions
Article Snippet: .. Agilent 2100 bioanalyzer instrument (Agilent techcologies, cat. no. G2940CA) Bio-Rad C1000 Thermal Cycler (Bio-rad, cat. no. 185-1148EDU) Biological safety cabinet classII, type A2 (Nuair, model no. NU-S480-600) Blue pippin instrument (Sage science, Blue Pippin) Centrifuge (Eppendorf 5810R, cat. no. 22628180) DynaMag™-2 Magnet (Life Technologies, cat. no. 12321D) Dark Reader Transilluminator (Clare Chemical Research, Inc. Model: DR89X) Eppendorf 5424 Ventilated Microcentrifuge (Eppendorf, cat. no. 022620401) Eppendorf Thermomixer (Eppendorf, cat. no. 5382000023) Freezer (Fisher Scientific, cat. no. 3752-DB) Illumina MiSeq and HiSeq2500 or NextSeq500 high-throughput sequencing machine (Illumina) Incubators (Heratherm, cat. no. 50125590) Intelli-mixer RM-2L (Rose scientific, cat. no. MX1000) Light cycle 480 PCR instrument (Roche, cat. no. 05015243001) MicroVac Concentrator (Tomy, cat. no. MV-100) Nikon TS100-F Microscope (Nikon, cat. no. TS100-F) Qubit 2.0 Fluorometer Specifications (Invitrogen, cat. no. ) Refrigerator (Fisher Scientific, cat. no. 13-994-201) Sonicator (SONICS & MATERIALS, cat. no. VCX130) Vortex-Genie® Mixers (VWR, cat. no. 58816-121) Water bath (Fisher Scientific, cat. no. 15-462-20Q) .. Cutadapt package (version 1.6) ( ) BWA software package (version 0.7.12-r1039 or above) ( ) SAMtools (version 0.1.19 and above) ( ) Picard tools (version 1.107 and above) ( ) BEDTools (version 2.17.0 and above) ( ) pysam python module (version 0.8.1) ( ) Perl scripts prepare_reads_single.pl (Supplementary Software 1) and prepare_reads_pair.pl (Supplementary Software 2) for bridge-linker trimming.

Polymerase Chain Reaction:

Article Title: Long-read ChIA-PET for base-pair resolution mapping of haplotype-specific chromatin interactions
Article Snippet: .. Agilent 2100 bioanalyzer instrument (Agilent techcologies, cat. no. G2940CA) Bio-Rad C1000 Thermal Cycler (Bio-rad, cat. no. 185-1148EDU) Biological safety cabinet classII, type A2 (Nuair, model no. NU-S480-600) Blue pippin instrument (Sage science, Blue Pippin) Centrifuge (Eppendorf 5810R, cat. no. 22628180) DynaMag™-2 Magnet (Life Technologies, cat. no. 12321D) Dark Reader Transilluminator (Clare Chemical Research, Inc. Model: DR89X) Eppendorf 5424 Ventilated Microcentrifuge (Eppendorf, cat. no. 022620401) Eppendorf Thermomixer (Eppendorf, cat. no. 5382000023) Freezer (Fisher Scientific, cat. no. 3752-DB) Illumina MiSeq and HiSeq2500 or NextSeq500 high-throughput sequencing machine (Illumina) Incubators (Heratherm, cat. no. 50125590) Intelli-mixer RM-2L (Rose scientific, cat. no. MX1000) Light cycle 480 PCR instrument (Roche, cat. no. 05015243001) MicroVac Concentrator (Tomy, cat. no. MV-100) Nikon TS100-F Microscope (Nikon, cat. no. TS100-F) Qubit 2.0 Fluorometer Specifications (Invitrogen, cat. no. ) Refrigerator (Fisher Scientific, cat. no. 13-994-201) Sonicator (SONICS & MATERIALS, cat. no. VCX130) Vortex-Genie® Mixers (VWR, cat. no. 58816-121) Water bath (Fisher Scientific, cat. no. 15-462-20Q) .. Cutadapt package (version 1.6) ( ) BWA software package (version 0.7.12-r1039 or above) ( ) SAMtools (version 0.1.19 and above) ( ) Picard tools (version 1.107 and above) ( ) BEDTools (version 2.17.0 and above) ( ) pysam python module (version 0.8.1) ( ) Perl scripts prepare_reads_single.pl (Supplementary Software 1) and prepare_reads_pair.pl (Supplementary Software 2) for bridge-linker trimming.

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  • 99
    Roche light cycler 480 instrument
    miRNA profiling of prostate cancer cells and effect of combination therapy on miRNA expression. Total RNA including miRNAs was isolated from DU145-TXR and DU145 cells (A) or PC3 and PC3-TXR cells (B) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C p  values and the ΔΔCt method. Table in the insert (C) confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by a SYBR Green based real time RT-PCR assay of selected miRNAs. As a normalizer, SNORD6 was used as a housekeeping miRNA. (D) Efficacy of combination therapy on restoration of miR 200 c and 34a was determined by treating DU145-TXR cells with PTX (0.5 µM) and CYA (10 µM)combination for 48 h after which total RNA was extracted, converted to cDNA and used as template for miScript primer assay for determining expression of miRNAs 200c and 34a. Untreated DU145-TXR cells were used as control for calculating fold change after a SYBR Green real time RT-PCR assay. Data represents mean ± SD (n = 3). *  p
    Light Cycler 480 Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light cycler 480 instrument/product/Roche
    Average 99 stars, based on 177 article reviews
    Price from $9.99 to $1999.99
    light cycler 480 instrument - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    81
    Roche applied science light cycler 480 0 instrument
    miRNA profiling of prostate cancer cells and effect of combination therapy on miRNA expression. Total RNA including miRNAs was isolated from DU145-TXR and DU145 cells (A) or PC3 and PC3-TXR cells (B) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C p  values and the ΔΔCt method. Table in the insert (C) confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by a SYBR Green based real time RT-PCR assay of selected miRNAs. As a normalizer, SNORD6 was used as a housekeeping miRNA. (D) Efficacy of combination therapy on restoration of miR 200 c and 34a was determined by treating DU145-TXR cells with PTX (0.5 µM) and CYA (10 µM)combination for 48 h after which total RNA was extracted, converted to cDNA and used as template for miScript primer assay for determining expression of miRNAs 200c and 34a. Untreated DU145-TXR cells were used as control for calculating fold change after a SYBR Green real time RT-PCR assay. Data represents mean ± SD (n = 3). *  p
    Applied Science Light Cycler 480 0 Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied science light cycler 480 0 instrument/product/Roche
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    applied science light cycler 480 0 instrument - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

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    miRNA profiling of prostate cancer cells and effect of combination therapy on miRNA expression. Total RNA including miRNAs was isolated from DU145-TXR and DU145 cells (A) or PC3 and PC3-TXR cells (B) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C p  values and the ΔΔCt method. Table in the insert (C) confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by a SYBR Green based real time RT-PCR assay of selected miRNAs. As a normalizer, SNORD6 was used as a housekeeping miRNA. (D) Efficacy of combination therapy on restoration of miR 200 c and 34a was determined by treating DU145-TXR cells with PTX (0.5 µM) and CYA (10 µM)combination for 48 h after which total RNA was extracted, converted to cDNA and used as template for miScript primer assay for determining expression of miRNAs 200c and 34a. Untreated DU145-TXR cells were used as control for calculating fold change after a SYBR Green real time RT-PCR assay. Data represents mean ± SD (n = 3). *  p

    Journal: PLoS ONE

    Article Title: Chemoresistance in Prostate Cancer Cells Is Regulated by miRNAs and Hedgehog Pathway

    doi: 10.1371/journal.pone.0040021

    Figure Lengend Snippet: miRNA profiling of prostate cancer cells and effect of combination therapy on miRNA expression. Total RNA including miRNAs was isolated from DU145-TXR and DU145 cells (A) or PC3 and PC3-TXR cells (B) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C p values and the ΔΔCt method. Table in the insert (C) confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by a SYBR Green based real time RT-PCR assay of selected miRNAs. As a normalizer, SNORD6 was used as a housekeeping miRNA. (D) Efficacy of combination therapy on restoration of miR 200 c and 34a was determined by treating DU145-TXR cells with PTX (0.5 µM) and CYA (10 µM)combination for 48 h after which total RNA was extracted, converted to cDNA and used as template for miScript primer assay for determining expression of miRNAs 200c and 34a. Untreated DU145-TXR cells were used as control for calculating fold change after a SYBR Green real time RT-PCR assay. Data represents mean ± SD (n = 3). * p

    Article Snippet: The plates were run on a Roche Light Cycler 480®instrument and the expression of individual miRNAs was analyzed using the obtained Ct values.

    Techniques: Expressing, Isolation, SYBR Green Assay, Polymerase Chain Reaction, Quantitative RT-PCR

    SP fraction analysis and miRNA profiling of clinical prostate tissues. (A) Human prostate cancer tissue was converted to single cell suspensions as described in ‘  Methods ’. Cells were stained with Hoechst dye and analyzed as described previously. Nearly 1% of total viable cell population was gated as the SP fraction. (B) Total RNA was isolated from another set of human prostate tissues (cancer and benign) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C t  values and the ΔΔCt method. The fold changes in the tumor tissues were normalized with respect to the benign prostate tissue. (C) Table in the insert confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by RT-PCR estimation of selected miRNAs200c, 34a and 29b. SNORD6 was used as a housekeeping miRNA for data normalization.

    Journal: PLoS ONE

    Article Title: Chemoresistance in Prostate Cancer Cells Is Regulated by miRNAs and Hedgehog Pathway

    doi: 10.1371/journal.pone.0040021

    Figure Lengend Snippet: SP fraction analysis and miRNA profiling of clinical prostate tissues. (A) Human prostate cancer tissue was converted to single cell suspensions as described in ‘ Methods ’. Cells were stained with Hoechst dye and analyzed as described previously. Nearly 1% of total viable cell population was gated as the SP fraction. (B) Total RNA was isolated from another set of human prostate tissues (cancer and benign) using miRNEasy RNA isolation kit. SYBR Green based pathway-focused miScript miRNA PCR Array (Qiagen, MD) was used for miRNA profiling studies. The plates were run on a Roche Light Cycler 480® instrument and the expression of individual miRNAs was analyzed using the obtained C t values and the ΔΔCt method. The fold changes in the tumor tissues were normalized with respect to the benign prostate tissue. (C) Table in the insert confirms validation of miRNA profiling data by miScript primer assay. Validation of miRNA profiling data was done by RT-PCR estimation of selected miRNAs200c, 34a and 29b. SNORD6 was used as a housekeeping miRNA for data normalization.

    Article Snippet: The plates were run on a Roche Light Cycler 480®instrument and the expression of individual miRNAs was analyzed using the obtained Ct values.

    Techniques: Staining, Isolation, SYBR Green Assay, Polymerase Chain Reaction, Expressing, Reverse Transcription Polymerase Chain Reaction