Structured Review

Olympus light brightfield microscope
Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in <t>brightfield</t> with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004
Light Brightfield Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/light brightfield microscope/product/Olympus
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
light brightfield microscope - by Bioz Stars, 2020-09
91/100 stars

Images

1) Product Images from "EM connectomics reveals axonal target variation in a sequence-generating network"

Article Title: EM connectomics reveals axonal target variation in a sequence-generating network

Journal: eLife

doi: 10.7554/eLife.24364

Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004
Figure Legend Snippet: Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004

Techniques Used: Sample Prep, Labeling, Microscopy, Staining

Related Articles

Microscopy:

Article Title: EM connectomics reveals axonal target variation in a sequence-generating network
Article Snippet: .. In brief, a transmitted light brightfield microscope (Olympus BX51, Olympus, Japan), equipped with a motorized x-y-z stage (Maerzhaeuser, Germany), a narrow bandpass (546 ± 5 nm) illumination filter and a 100x magnification oil-immersion objective (numerical aperture 1.4) was used to acquire image stacks from consecutive 100 µm thick brain sections. ..

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  • 92
    Olympus brightfield microscopy
    Delivery efficiency as determined by the percentage of the volume of microneedles that dissolved during microneedle patch application to the skin. Microneedle patches were imaged by <t>brightfield</t> microscopy before and after insertion into the skin and image analysis was used to determine the volume of microneedles dissolved. (a) Volume of microneedles dissolved is interpreted as a measure of the dose delivered, i.e., if a drug or vaccine had been incorporated into the full volume of the microneedles. Each bar represents the result from an individual subject. The data are presented in descending order of investigator-administered delivery efficiency. The ‘average’ bars represent the averages of the 15 individual bars, with standard deviation error bars shown. (b) Percent of subjects that delivered more than 50% of the volume of the microneedles.
    Brightfield Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brightfield microscopy/product/Olympus
    Average 92 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    brightfield microscopy - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Olympus brightfield illumination
    Labeled terminals and boutons from ACC axons terminate in the olfactory AON. a , Darkfield photomicrograph shows dense patch of anterograde label (white grain, white arrow) found at the level of AONd extending to the adjacent orbitofrontal area OPAll (case DQ, tracer 3 H-labeled amino acids). b , Darkfield photomicrograph shows dense patches of labeled axons (white patch, black arrow) in the same location as in A, and in the adjacent orbital cortex in another case (case BI, tracer BDA); white arrowheads point to two layers of small vessels that mark the limit between layer I of AONd and layer I of orbital area OPAll. c , <t>Brightfield</t> photomicrographs at higher magnification show labeled axons and boutons (brown dots) in layer I of AON (case AY, tracer BDA); and d , in the islands of Calleja of the olfactory TOL 2 (case BG, tracer BDA). The insets in C and D show en passant (black arrow heads) and terminaux (white arrow heads) boutons on labeled axons. Photomicrographs are from coronal sections through the primary olfactory cortex. Calibration bar in b applies to a and b. Calibrations bars in d apply to c and d.
    Brightfield Illumination, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brightfield illumination/product/Olympus
    Average 91 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    brightfield illumination - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    91
    Olympus light brightfield microscope
    Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in <t>brightfield</t> with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004
    Light Brightfield Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light brightfield microscope/product/Olympus
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    light brightfield microscope - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Delivery efficiency as determined by the percentage of the volume of microneedles that dissolved during microneedle patch application to the skin. Microneedle patches were imaged by brightfield microscopy before and after insertion into the skin and image analysis was used to determine the volume of microneedles dissolved. (a) Volume of microneedles dissolved is interpreted as a measure of the dose delivered, i.e., if a drug or vaccine had been incorporated into the full volume of the microneedles. Each bar represents the result from an individual subject. The data are presented in descending order of investigator-administered delivery efficiency. The ‘average’ bars represent the averages of the 15 individual bars, with standard deviation error bars shown. (b) Percent of subjects that delivered more than 50% of the volume of the microneedles.

    Journal: Biomaterials

    Article Title: Tolerability, usability and acceptability of dissolving microneedle patch administration in human subjects

    doi: 10.1016/j.biomaterials.2017.02.040

    Figure Lengend Snippet: Delivery efficiency as determined by the percentage of the volume of microneedles that dissolved during microneedle patch application to the skin. Microneedle patches were imaged by brightfield microscopy before and after insertion into the skin and image analysis was used to determine the volume of microneedles dissolved. (a) Volume of microneedles dissolved is interpreted as a measure of the dose delivered, i.e., if a drug or vaccine had been incorporated into the full volume of the microneedles. Each bar represents the result from an individual subject. The data are presented in descending order of investigator-administered delivery efficiency. The ‘average’ bars represent the averages of the 15 individual bars, with standard deviation error bars shown. (b) Percent of subjects that delivered more than 50% of the volume of the microneedles.

    Article Snippet: To measure delivery efficiency, microneedle patches were imaged using brightfield microscopy (SZX12 Olympus, Center Valley, PA) before and after administration, and the microneedle dimensions were measured to calculate the volume dissolved after microneedle patch administration.

    Techniques: Microscopy, Standard Deviation

    Neither EPO nor EPO-R76E induce tube formation in HRMECs. A–E) Brightfield micrographs of HRMECs treated with A) 10% serum, B) 0% serum, or C) commercial EPO, D) EPO, or E) EPO-R76E in 0% serum. F) Bar graph quantification of lengths of tube walls in each condition. Error bars indicate SEM.

    Journal: Gene therapy

    Article Title: Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin

    doi: 10.1038/gt.2015.12

    Figure Lengend Snippet: Neither EPO nor EPO-R76E induce tube formation in HRMECs. A–E) Brightfield micrographs of HRMECs treated with A) 10% serum, B) 0% serum, or C) commercial EPO, D) EPO, or E) EPO-R76E in 0% serum. F) Bar graph quantification of lengths of tube walls in each condition. Error bars indicate SEM.

    Article Snippet: Histology Additional cryo-sections were stained with hematoxylin and eosin (Fisher) and brightfield microscopy was performed on an Olympus Provis AX70 microscope (n=15 per treatment group).

    Techniques:

    Labeled terminals and boutons from ACC axons terminate in the olfactory AON. a , Darkfield photomicrograph shows dense patch of anterograde label (white grain, white arrow) found at the level of AONd extending to the adjacent orbitofrontal area OPAll (case DQ, tracer 3 H-labeled amino acids). b , Darkfield photomicrograph shows dense patches of labeled axons (white patch, black arrow) in the same location as in A, and in the adjacent orbital cortex in another case (case BI, tracer BDA); white arrowheads point to two layers of small vessels that mark the limit between layer I of AONd and layer I of orbital area OPAll. c , Brightfield photomicrographs at higher magnification show labeled axons and boutons (brown dots) in layer I of AON (case AY, tracer BDA); and d , in the islands of Calleja of the olfactory TOL 2 (case BG, tracer BDA). The insets in C and D show en passant (black arrow heads) and terminaux (white arrow heads) boutons on labeled axons. Photomicrographs are from coronal sections through the primary olfactory cortex. Calibration bar in b applies to a and b. Calibrations bars in d apply to c and d.

    Journal: Brain structure & function

    Article Title: A direct anterior cingulate pathway to the primate primary olfactory cortex may control attention to olfaction

    doi: 10.1007/s00429-013-0598-3

    Figure Lengend Snippet: Labeled terminals and boutons from ACC axons terminate in the olfactory AON. a , Darkfield photomicrograph shows dense patch of anterograde label (white grain, white arrow) found at the level of AONd extending to the adjacent orbitofrontal area OPAll (case DQ, tracer 3 H-labeled amino acids). b , Darkfield photomicrograph shows dense patches of labeled axons (white patch, black arrow) in the same location as in A, and in the adjacent orbital cortex in another case (case BI, tracer BDA); white arrowheads point to two layers of small vessels that mark the limit between layer I of AONd and layer I of orbital area OPAll. c , Brightfield photomicrographs at higher magnification show labeled axons and boutons (brown dots) in layer I of AON (case AY, tracer BDA); and d , in the islands of Calleja of the olfactory TOL 2 (case BG, tracer BDA). The insets in C and D show en passant (black arrow heads) and terminaux (white arrow heads) boutons on labeled axons. Photomicrographs are from coronal sections through the primary olfactory cortex. Calibration bar in b applies to a and b. Calibrations bars in d apply to c and d.

    Article Snippet: We studied the distribution of anterograde label under brightfield illumination (Olympus optical microscope, BX 60).

    Techniques: Labeling

    Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004

    Journal: eLife

    Article Title: EM connectomics reveals axonal target variation in a sequence-generating network

    doi: 10.7554/eLife.24364

    Figure Lengend Snippet: Sample preparation for LM and EM. ( a,b ) For LM, HVC (RA) neurons are retrogradely labeled by injecting a fluorescent dextran (fluoro-Ruby) into RA ( a ), and a single labeled neuron is targeted and filled with Neurobiotin under the guidance of 2-photon microscopy ( b ). ( c ) The tissue is then fixed and 100 µm parasagittal sections are sliced through the entirety of HVC. ( d,e ) Sections are further processed to stain Neurobiotin-labeled neurites ( d ) and then imaged in brightfield with a 100x objective ( e ). ( f ) For EM, HVC (RA) neurons are retrogradely labeled with biotinylated dextran (BDA). ( g,h ) A single 200 µm parasagittal section is taken from the center of HVC ( g ) and further processed to stain BDA-labeled neurites ( h ). ( i,j ) A cube of tissue from within the center of HVC is extracted and stained by ROTO (reduced osmium OTO) ( i ) before being imaged via SBEM ( j ). DOI: http://dx.doi.org/10.7554/eLife.24364.004

    Article Snippet: In brief, a transmitted light brightfield microscope (Olympus BX51, Olympus, Japan), equipped with a motorized x-y-z stage (Maerzhaeuser, Germany), a narrow bandpass (546 ± 5 nm) illumination filter and a 100x magnification oil-immersion objective (numerical aperture 1.4) was used to acquire image stacks from consecutive 100 µm thick brain sections.

    Techniques: Sample Prep, Labeling, Microscopy, Staining