ligation sequencing kit  (New England Biolabs)


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    Structured Review

    New England Biolabs ligation sequencing kit
    Ligation Sequencing Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligation sequencing kit/product/New England Biolabs
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ligation sequencing kit - by Bioz Stars, 2020-02
    88/100 stars

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    Related Articles

    Selection:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: Paragraph title: Cap selection, cDNA synthesis and sequencing ... The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends.

    Ligation:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: .. The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends. .. This step was followed by the 1D adapter ligation, which was carried out according to the 1D protocol, using the NEB Blunt/TA Ligase Master Mix (New England Biolabs).

    Sequencing:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: .. The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends. .. This step was followed by the 1D adapter ligation, which was carried out according to the 1D protocol, using the NEB Blunt/TA Ligase Master Mix (New England Biolabs).

    Amplification:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: In order to generate sufficient amount of cDNAs for MinION library preparation, samples were amplified by endpoint PCR following the TeloPrime Kit’s manual. .. The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends.

    In Silico:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends. .. We used barcoding for the better in silico identification of the transcripts’ ends.

    Polymerase Chain Reaction:

    Article Title: Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
    Article Snippet: In order to generate sufficient amount of cDNAs for MinION library preparation, samples were amplified by endpoint PCR following the TeloPrime Kit’s manual. .. The Ligation Sequencing kit (SQK-LSK108, ONT) and NEBNext End repair / dA-tailing Module (New England Biolabs) was used to repair the cDNA ends.

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    New England Biolabs quick ligation kit
    Quick Ligation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick ligation kit/product/New England Biolabs
    Average 95 stars, based on 223 article reviews
    Price from $9.99 to $1999.99
    quick ligation kit - by Bioz Stars, 2020-02
    95/100 stars
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