primestore molecular transport medium (Longhorn Vaccines and Diagnostics LLC)

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Longhorn Vaccines and Diagnostics LLC primestore molecular transport medium
Primestore Molecular Transport Medium, supplied by Longhorn Vaccines and Diagnostics LLC, used in various techniques. Bioz Stars score: 96/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lh-psmtm-5/PrimeStore+MTM/pmc13155352-351-7-11
Average 96 stars, based on 285 article reviews

primestore molecular transport medium - by Bioz Stars, 2026-09

96/100 stars

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Quantitative RT-PCR:

Article Title: Pilot Assessment of RNA Stabilization Methods for Influenza A Virus in Swine Oral Fluids
Article Snippet: .. Thus, the objective of this study was to compare molecular biology-grade carbohydrates (trehalose, sorbitol, sucrose, and mannitol) and two commercial storage products: PrimeStore® MTM (Longhorn Vaccines and Diagnostics, Bethesda, MD, USA) and RNAlater® (Thermo Fisher Scientific, Waltham, MA, USA), in terms of their capacity to preserve RT-qPCR detectable IAV RNA in swine oral fluid samples exposed to less-thanideal storage conditions. .. Two studies were performed to evaluate the detection of IAV RNA in oral fluid samples treated with molecular-grade carbohydrates (20% w/v), i.e., (1) trehalose, (2) sorbitol, (3) sucrose, (4) mannitol, and commercial nucleic acid stabilizers: (5) PrimeStore® MTM (Longhorn Vaccines and Diagnostics, Bethesda, MD, USA), and (6) RNAlater® (Thermo Fisher Scientific, Waltham, MA, USA).

Extraction:

Article Title: Culturally acceptable fermented grain may improve gut health in South African postpartum mothers in a randomised trial
Article Snippet: .. Nucleic acid extraction, 16S rRNA gene amplification and sequencing of mageu and maternal stool DNA was extracted from 400 μl of mageu stored in a 1:1 ratio in Primestore® Molecular Transport Medium (Longhorn Vaccines & Diagnostics LLC) and from 200 mg of dry maternal stool collected at weeks 4, 10 and 15, using the DNeasy Powersoil Pro kit (Qiagen) following the manufacturer’s protocol, with mechanical disruption by bead-beating at 50 Hz for 10 min (Qiagen TissueLyser LT). ..

Amplification:

Sequencing:

Disruption:

Nucleic Acid Purification:

Article Title: Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Interlaboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability.
Article Snippet: .. Briefly, samples were diluted 1:3 in PrimeStore Molecular Transport Medium (MTM) (Longhorn Vaccine and Diagnostics) and nucleic acids extracted using MagMAX CORE Nucleic Acid Purification Kit on a Kingfisher Flex instrument (Kingfisher Flex, Life Technologies Holdings Pte Ltd, Singapore). .. The purified RNA was reverse transcribed to cDNA and amplified with primers and probes targeting the IAV matrix gene (IAV M) (Integrated DNA Technologies, IDT) using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher).

Milk:

Article Title: Mammary and respiratory infection of sheep with H5Nx clade 2.3.4.4b viruses with milk-mediated transmission to lambs
Article Snippet: .. Milk samples were diluted 1:3 (v/v) in PrimeStore Molecular Transport Medium (Longhorn Vaccines and Diagnostics LLC of Bethesda, MD). .. Tissue samples were homogenized in 1× phosphate-buffered saline using a Precellys 24 Touch Homogenizer (Bertin Technologies) for two cycles of 20 s each, generating 10% (w/v) homogenates, which were immediately placed on ice.

other:

Article Title: Pilot Assessment of RNA Stabilization Methods for Influenza A Virus in Swine Oral Fluids
Article Snippet: Two studies were performed to evaluate the detection of IAV RNA in oral fluid samples treated with molecular-grade carbohydrates (20% w/v), i.e., (1) trehalose, (2) sorbitol, (3) sucrose, (4) mannitol, and commercial nucleic acid stabilizers: (5) PrimeStore® MTM (Longhorn Vaccines and Diagnostics, Bethesda, MD, USA), and (6) RNAlater® (Thermo Fisher Scientific, Waltham, MA, USA).

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