Structured Review

Peptides International leupeptin
Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor <t>(leupeptin)</t> (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p
Leupeptin, supplied by Peptides International, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/Peptides International
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "β-Adrenergic receptor trafficking, degradation, and cell-surface expression are altered in dermal fibroblasts from hypertrophic scars"

Article Title: β-Adrenergic receptor trafficking, degradation, and cell-surface expression are altered in dermal fibroblasts from hypertrophic scars

Journal: The Journal of investigative dermatology

doi: 10.1016/j.jid.2018.01.037

Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor (leupeptin) (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p
Figure Legend Snippet: Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor (leupeptin) (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p

Techniques Used: Western Blot, Expressing

Effect of burn trauma on catecholamine-stimulated trafficking and degradation of β2-AR in human dermal fibroblasts Dermal fibroblasts were plated on glass coverslips one day before transfection with the 10 μg/ml ß2-AR–GFP construct or control vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA). a. Immunofluorescent analysis of NF, HSF, and NSF fibroblasts transfected with ß2-AR–GFP (green) and stimulated for 6 hours with isoproterenol (ISO) or ISO with propranolol (PPL). Lysosomal compartments were visualized with the anti-LAMP2A (red). The bottom two rows show the effect of proteasome (MG132) and lysosome inhibition (Leupeptin-Leup) on ISO-stimulated changes in ß2-AR–GFP trafficking. Arrows point to the areas of colocalization of ß2-AR-GFP to the lysosomes. These experiments have been repeated at least 3 times with 15-20 cells imaged in each preparation. Scale bar, 10 μm. b. Quantification of colocalization (Icorr), presented as the mean ± SEM of 9 separate cells. *p
Figure Legend Snippet: Effect of burn trauma on catecholamine-stimulated trafficking and degradation of β2-AR in human dermal fibroblasts Dermal fibroblasts were plated on glass coverslips one day before transfection with the 10 μg/ml ß2-AR–GFP construct or control vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA). a. Immunofluorescent analysis of NF, HSF, and NSF fibroblasts transfected with ß2-AR–GFP (green) and stimulated for 6 hours with isoproterenol (ISO) or ISO with propranolol (PPL). Lysosomal compartments were visualized with the anti-LAMP2A (red). The bottom two rows show the effect of proteasome (MG132) and lysosome inhibition (Leupeptin-Leup) on ISO-stimulated changes in ß2-AR–GFP trafficking. Arrows point to the areas of colocalization of ß2-AR-GFP to the lysosomes. These experiments have been repeated at least 3 times with 15-20 cells imaged in each preparation. Scale bar, 10 μm. b. Quantification of colocalization (Icorr), presented as the mean ± SEM of 9 separate cells. *p

Techniques Used: Transfection, Construct, Plasmid Preparation, Inhibition

Related Articles

Lysis:

Article Title: Varicellovirus UL49.5 Proteins Differentially Affect the Function of the Transporter Associated with Antigen Processing, TAP
Article Snippet: .. To determine steady state protein levels, cells were lysed in Nonidet P-40 (NP-40) lysis mix containing 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2 and 0.5% NP-40, supplemented with 1 mM AEBSF (4-(2-Aminoethyl)-benzenesulfonyl fluoride), 1 mM leupeptin and 20 µM Cbz-L3 (Carbobenzoxy-1-Leucyl-1-Leucyl-1-Leucinal-H; Peptides International, Inc). ..

Incubation:

Article Title: Cytokine-induced F-actin reorganization in endothelial cells involves RhoA activation
Article Snippet: .. One milliliter of cold extraction buffer A [25 mM Tris (pH 7.5 at 4°C), 150 mM potassium acetate, 5 mM EDTA, 5 mM EGTA, 10 mM DTT, 1% Triton X-100, 60 mM n -octyl-β- d -glucopyranoside, 50 μM butylated hydroxytoluene, 1 mM PMSF, 1 mM benzamidine, 2 μg/ml aprotinin (Roche Molecular Biochemicals, Indianapolis, IN), 1 μg/ml pepstatin A (Roche Molecular Biochemicals), 50 μg/ml leupeptin (Peptides International, Osaka, Japan), and 40 μg/ml bestatin (Roche Molecular Biochemicals)] was added to the 100-mm plate and incubated on ice for 10 min with periodic manual rocking to evenly distribute the extraction buffer. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Peptides International leupeptin
    Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor <t>(leupeptin)</t> (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p
    Leupeptin, supplied by Peptides International, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/Peptides International
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Peptides International leupeptin hemisulfate
    Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor <t>(leupeptin)</t> (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p
    Leupeptin Hemisulfate, supplied by Peptides International, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin hemisulfate/product/Peptides International
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leupeptin hemisulfate - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    Image Search Results


    Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor (leupeptin) (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p

    Journal: The Journal of investigative dermatology

    Article Title: β-Adrenergic receptor trafficking, degradation, and cell-surface expression are altered in dermal fibroblasts from hypertrophic scars

    doi: 10.1016/j.jid.2018.01.037

    Figure Lengend Snippet: Propranolol (PPL) modulates ß-AR degradation in human dermal fibroblasts Twenty-four hours after being plated, NF, HSF, and NSF fibroblasts were pretreated for 30 minutes with PPL or vehicle and then treated for the indicated time with a proteasome inhibitor (MG132) (10 μM) or lysosomal inhibitor (leupeptin) (50 μM). Western blotting was performed to determine the expression of ß1-, ß2-, and ß3-ARs in the presence of a. vehicle or b. PPL (10 μM). c. Quantification of ß1-AR band intensity. d. Quantification of ß2-AR band intensity. e. Quantification of ß3-AR band intensity. Data were normalized to control and were presented as mean ± SEM of at least 3 independent experiments. *p

    Article Snippet: Dermal fibroblasts were treated with MG132 (Calbiochem, Millipore, MA, USA) or Leupeptin (Peptides International, Louisville, KY, USA) ( , ).

    Techniques: Western Blot, Expressing

    Effect of burn trauma on catecholamine-stimulated trafficking and degradation of β2-AR in human dermal fibroblasts Dermal fibroblasts were plated on glass coverslips one day before transfection with the 10 μg/ml ß2-AR–GFP construct or control vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA). a. Immunofluorescent analysis of NF, HSF, and NSF fibroblasts transfected with ß2-AR–GFP (green) and stimulated for 6 hours with isoproterenol (ISO) or ISO with propranolol (PPL). Lysosomal compartments were visualized with the anti-LAMP2A (red). The bottom two rows show the effect of proteasome (MG132) and lysosome inhibition (Leupeptin-Leup) on ISO-stimulated changes in ß2-AR–GFP trafficking. Arrows point to the areas of colocalization of ß2-AR-GFP to the lysosomes. These experiments have been repeated at least 3 times with 15-20 cells imaged in each preparation. Scale bar, 10 μm. b. Quantification of colocalization (Icorr), presented as the mean ± SEM of 9 separate cells. *p

    Journal: The Journal of investigative dermatology

    Article Title: β-Adrenergic receptor trafficking, degradation, and cell-surface expression are altered in dermal fibroblasts from hypertrophic scars

    doi: 10.1016/j.jid.2018.01.037

    Figure Lengend Snippet: Effect of burn trauma on catecholamine-stimulated trafficking and degradation of β2-AR in human dermal fibroblasts Dermal fibroblasts were plated on glass coverslips one day before transfection with the 10 μg/ml ß2-AR–GFP construct or control vector using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA). a. Immunofluorescent analysis of NF, HSF, and NSF fibroblasts transfected with ß2-AR–GFP (green) and stimulated for 6 hours with isoproterenol (ISO) or ISO with propranolol (PPL). Lysosomal compartments were visualized with the anti-LAMP2A (red). The bottom two rows show the effect of proteasome (MG132) and lysosome inhibition (Leupeptin-Leup) on ISO-stimulated changes in ß2-AR–GFP trafficking. Arrows point to the areas of colocalization of ß2-AR-GFP to the lysosomes. These experiments have been repeated at least 3 times with 15-20 cells imaged in each preparation. Scale bar, 10 μm. b. Quantification of colocalization (Icorr), presented as the mean ± SEM of 9 separate cells. *p

    Article Snippet: Dermal fibroblasts were treated with MG132 (Calbiochem, Millipore, MA, USA) or Leupeptin (Peptides International, Louisville, KY, USA) ( , ).

    Techniques: Transfection, Construct, Plasmid Preparation, Inhibition