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Bio-Rad leupeptin
Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, <t>leupeptin</t> and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.
Leupeptin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/Bio-Rad
Average 92 stars, based on 87 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "N-Terminal Truncation of Stat5a/b Circumvents PIAS3-Mediated Transcriptional Inhibition of Stat5 in Prostate Cancer Cells"

Article Title: N-Terminal Truncation of Stat5a/b Circumvents PIAS3-Mediated Transcriptional Inhibition of Stat5 in Prostate Cancer Cells

Journal: The international journal of biochemistry & cell biology

doi: 10.1016/j.biocel.2010.09.008

Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, leupeptin and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.
Figure Legend Snippet: Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, leupeptin and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.

Techniques Used: Electro Mobility Shift Assay, Generated, In Vitro, Lysis, Western Blot

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Centrifugation:

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Homogenization:

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Bradford Assay:

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Western Blot:

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Incubation:

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Expressing:

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Protein Concentration:

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Sonication:

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Lysis:

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Article Title: Transcription Factor Stat3 Stimulates Metastatic Behavior of Human Prostate Cancer Cells in Vivo, whereas Stat5b Has a Preferential Role in the Promotion of Prostate Cancer Cell Viability and Tumor Growth
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Article Title: N-Terminal Truncation of Stat5a/b Circumvents PIAS3-Mediated Transcriptional Inhibition of Stat5 in Prostate Cancer Cells
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    Bio-Rad leupeptin
    Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, <t>leupeptin</t> and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.
    Leupeptin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/Bio-Rad
    Average 92 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, leupeptin and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.

    Journal: The international journal of biochemistry & cell biology

    Article Title: N-Terminal Truncation of Stat5a/b Circumvents PIAS3-Mediated Transcriptional Inhibition of Stat5 in Prostate Cancer Cells

    doi: 10.1016/j.biocel.2010.09.008

    Figure Lengend Snippet: Short form STAT5 occurs naturally in prostate cancer cells A , Electromobility shift assay of nuclear extract of DU145 cells prepared from flash frozen cell pellets in the absence (lane 1 and 2) or presence (lane 3 and 4) of protease inhibitors (phenylmethylsulfonylfluoride, aprotinin, leupeptin and pepstatin-A). The short form Stat5a/b (arrow) was detected in DU145 cells in the presence or in the absence of protease inhibitors, and the short form Stat5a/b was super-shifted by anti-Stat5a and anti-Stat5b antibodies. B , The short form Stat5a/b is not generated in vitro after disruption of the cell membranes. DU145 cell pellets were flash frozen in liquid nitrogen and directly lysed in boiling SDS loading buffer (lane 1), or the cell pellets were lysed in cell lysis buffer in the presence (lane 2) or in the absence of proteases inhibitors (lane 3) and boiled in SDS loading buffer and analyzed by Western blotting using anti-C-terminus Stat5a/b mAb.

    Article Snippet: Cells were lysed and fresh human prostate cancer specimens were homogenized in lysis buffer [10 mM Tris-HCl (pH 7.5), 5 mM EDTA, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM sodium fluoride, 1 mM sodium orthovanadate, 1% Triton X-100, 1 mM phenylmethylsulphonylfluoride, 5 μg/ml aprotinin, 1 μg/ml pepstatin A, and 2 μg/ml leupeptin], and the protein concentrations of the whole cell lysates were determined by the Bradford method (BioRad Laboratories Inc., Hercules, CA).

    Techniques: Electro Mobility Shift Assay, Generated, In Vitro, Lysis, Western Blot