lentiviral vectors targeting tmem16f  (Santa Cruz Biotechnology)

 
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    Name:
    ANO6 shRNA m Lentiviral Particles
    Description:
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of ANO6 gene silencing results individual duplex components or plasmids are also available upon request
    Catalog Number:
    SC-154404-V
    Price:
    None
    Category:
    Gene Editing siRNA shRNA Gene Silencers Signaling Intermediate ANO6 siRNA shRNA Plasmid and Lentiviral Particle Gene Silencers ANO6 siRNA and shRNA Plasmids m
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    Structured Review

    Santa Cruz Biotechnology lentiviral vectors targeting tmem16f
    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F <t>(TMEM16F)</t> function. Huh7 cells were transduced with <t>lentiviral</t> vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of ANO6 gene silencing results individual duplex components or plasmids are also available upon request
    https://www.bioz.com/result/lentiviral vectors targeting tmem16f/product/Santa Cruz Biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lentiviral vectors targeting tmem16f - by Bioz Stars, 2021-09
    91/100 stars

    Images

    1) Product Images from "Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions"

    Article Title: Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jiy485

    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.
    Figure Legend Snippet: Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Techniques Used: Expressing, Transduction, shRNA, Infection, Translocation Assay, Imaging, Flow Cytometry, Cytometry, Staining

    2) Product Images from "Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions"

    Article Title: Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions

    Journal: The Journal of Infectious Diseases

    doi: 10.1093/infdis/jiy485

    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.
    Figure Legend Snippet: Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Techniques Used: Expressing, Transduction, shRNA, Infection, Translocation Assay, Imaging, Flow Cytometry, Cytometry, Staining

    Related Articles

    Transduction:

    Article Title: Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions
    Article Snippet: .. Lentiviral vectors targeting TMEM16F and XKr8 (both from Santa Cruz Biotechnology) and scrambled short hairpin RNAs (shRNAs) (Thermo Fisher Scientific) were used to transduce Huh7 cells in 6-well plates. ..

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    Santa Cruz Biotechnology lentiviral vectors targeting tmem16f
    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F <t>(TMEM16F)</t> function. Huh7 cells were transduced with <t>lentiviral</t> vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.
    Lentiviral Vectors Targeting Tmem16f, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral vectors targeting tmem16f/product/Santa Cruz Biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lentiviral vectors targeting tmem16f - by Bioz Stars, 2021-09
    91/100 stars
      Buy from Supplier

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    Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Journal: The Journal of Infectious Diseases

    Article Title: Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions

    doi: 10.1093/infdis/jiy485

    Figure Lengend Snippet: Ebola virus (EBOV)–induced extracellular expression of phosphatidylserine (PtdSer) is dependent on transmembrane protein 16F (TMEM16F) function. Huh7 cells were transduced with lentiviral vectors encoding scrambled short hairpin RNA (shRNA) (Scr) or shRNAs specific for TMEM16F and infected with EBOV at a multiplicity of infection of 1 plaque-forming unit/cell. A, B, Translocation of PtdSer to the outer leaflet of the plasma membrane in EBOV-infected Huh7/shScramble ( A ) or Huh7/shTMEM16F ( B ) cells. Targeted knockdown of TMEM16F results in decreased levels of plasma membrane-associated PtdSer (scale bar represents 30 μm). C–E, Imaging flow cytometry analysis of mock-infected and EBOV-infected shRNA cell lines. C, Representative images of mock-infected and EBOV-infected Huh7/shScramble and Huh7/shTMEM16F cell lines. The merged image is based on glycoprotein (GP), annexin V, and 4′,6-diamidino-2-phenylindole (DAPI). D, Relative levels of EBOV GP ( left ) and annexin V staining ( right for gating strategy. Panels A and B are representative of 1 of 7 and panels C and D are representative of 1 of 3 independent experiments. Abbreviations: BF, bright field; SSC, side scatter.

    Article Snippet: Lentiviral vectors targeting TMEM16F and XKr8 (both from Santa Cruz Biotechnology) and scrambled short hairpin RNAs (shRNAs) (Thermo Fisher Scientific) were used to transduce Huh7 cells in 6-well plates.

    Techniques: Expressing, Transduction, shRNA, Infection, Translocation Assay, Imaging, Flow Cytometry, Cytometry, Staining