lectin ricinus communis agglutinin 1 rca 1  (Vector Laboratories)


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    Vector Laboratories lectin ricinus communis agglutinin 1 rca 1
    Lectin Ricinus Communis Agglutinin 1 Rca 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated lectin ricinus communis agglutinin 1  (Vector Laboratories)


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    Vector Laboratories biotinylated lectin ricinus communis agglutinin 1
    Immunohistochemical illustration of Ricinus communis <t>agglutinin-1</t> <t>(RCA-1)</t> -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.
    Biotinylated Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans"

    Article Title: Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-7-7

    Immunohistochemical illustration of Ricinus communis agglutinin-1 (RCA-1) -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.
    Figure Legend Snippet: Immunohistochemical illustration of Ricinus communis agglutinin-1 (RCA-1) -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.

    Techniques Used: Immunohistochemical staining

    lectin ricinus communis agglutinin 1 rca 1  (Vector Laboratories)


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    Vector Laboratories lectin ricinus communis agglutinin 1 rca 1
    Lectin Ricinus Communis Agglutinin 1 Rca 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i rca 1 lectins  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i rca 1 lectins
    Ricinus Communis Agglutinin I Rca 1 Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i rca 1 lectins  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i rca 1 lectins
    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on <t>RCA-1</t> binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
    Ricinus Communis Agglutinin I Rca 1 Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia"

    Article Title: Platelet Desialylation Is a Novel Mechanism and Therapeutic Target in Daboia siamensis and Agkistrodon halys Envenomation-Induced Thrombocytopenia

    Journal: Molecules

    doi: 10.3390/molecules27227779

    Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.
    Figure Legend Snippet: Viperid snake venoms induces platelet desialylation. ( A , B ) Effect of the venoms from D. siamensis (DSV), A. halys (AHV) and B. multicinctus (BMV) on RCA-1 binding. Effect of OSGE and oseltamivir on ( C ) DSV and ( D ) AHV venoms induced RCA-1 binding. Statistical analysis was performed with the one-way ANOVA test. n = 3. *** p < 0.001.

    Techniques Used: Binding Assay

    lectin ricinus communis agglutinin 1  (Vector Laboratories)


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    Vector Laboratories lectin ricinus communis agglutinin 1
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination"

    Article Title: Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination

    Journal: Pharmaceuticals

    doi: 10.3390/ph14111156

    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Figure Legend Snippet: Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Techniques Used:

    fluorescein ricinus communis agglutinin 1 lectin  (Vector Laboratories)


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    Vector Laboratories fluorescein ricinus communis agglutinin 1 lectin

    Fluorescein Ricinus Communis Agglutinin 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells"

    Article Title: Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2021.100678


    Figure Legend Snippet:

    Techniques Used: Recombinant, Staining, Plasmid Preparation, Software, Phagocytosis Assay, Microscopy

    rhodamine labeled ricinus communis agglutinin i (rca i, rca120)  (Vector Laboratories)


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    Vector Laboratories rhodamine labeled ricinus communis agglutinin i (rca i, rca120)
    Rhodamine Labeled Ricinus Communis Agglutinin I (Rca I, Rca120), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin 1 rca 1 lectin  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin 1 rca 1 lectin
    NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis <t>Agglutinin-1);</t> Magnification: 20X, scale bar: 100 µm.
    Ricinus Communis Agglutinin 1 Rca 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Differential Expression Profile of NLRs and AIM2 in Glioma and Implications for NLRP12 in Glioblastoma"

    Article Title: Differential Expression Profile of NLRs and AIM2 in Glioma and Implications for NLRP12 in Glioblastoma

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-44854-4

    NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis Agglutinin-1); Magnification: 20X, scale bar: 100 µm.
    Figure Legend Snippet: NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis Agglutinin-1); Magnification: 20X, scale bar: 100 µm.

    Techniques Used: Inhibition, Expressing, Immunofluorescence, Colony Assay, Migration, Staining

    streptavidin labeled ricinus communis agglutinin 1 rca 1 lectin  (Vector Laboratories)


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    Vector Laboratories streptavidin labeled ricinus communis agglutinin 1 rca 1 lectin
    Streptavidin Labeled Ricinus Communis Agglutinin 1 Rca 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    streptavidin labeled ricinus communis agglutinin 1 rca 1 lectin  (Vector Laboratories)


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    Vector Laboratories streptavidin labeled ricinus communis agglutinin 1 rca 1 lectin
    Streptavidin Labeled Ricinus Communis Agglutinin 1 Rca 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories lectin ricinus communis agglutinin 1 rca 1
    Lectin Ricinus Communis Agglutinin 1 Rca 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated lectin ricinus communis agglutinin 1
    Immunohistochemical illustration of Ricinus communis <t>agglutinin-1</t> <t>(RCA-1)</t> -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.
    Biotinylated Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories ricinus communis agglutinin i rca 1 lectins
    Immunohistochemical illustration of Ricinus communis <t>agglutinin-1</t> <t>(RCA-1)</t> -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.
    Ricinus Communis Agglutinin I Rca 1 Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories ricinus communis agglutinin 1 rca 1 lectin
    NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis <t>Agglutinin-1);</t> Magnification: 20X, scale bar: 100 µm.
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    Vector Laboratories streptavidin labeled ricinus communis agglutinin 1 rca 1 lectin
    NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis <t>Agglutinin-1);</t> Magnification: 20X, scale bar: 100 µm.
    Streptavidin Labeled Ricinus Communis Agglutinin 1 Rca 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunohistochemical illustration of Ricinus communis agglutinin-1 (RCA-1) -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.

    Journal: Journal of Neuroinflammation

    Article Title: Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans

    doi: 10.1186/1742-2094-7-7

    Figure Lengend Snippet: Immunohistochemical illustration of Ricinus communis agglutinin-1 (RCA-1) -positive microglial cells in the white matter of spinal cord . The RCA-1-positive microglial cells (arrowheads) in both healthy (A) and AvIDP-affected (B) chicken show incospicuous ramified microglial cells. Activated microglial cells or an increased number were not observed. A, B: asterisk = endothelium (also stains RCA-1 positive); scale bar = 250 μm.

    Article Snippet: Furthermore, the biotinylated lectin Ricinus communis agglutinin-1 (RCA 120, 1:3000; Vector Laboratories, CA, USA) was used as a specific marker for microglial cells within the spinal cord [ ].

    Techniques: Immunohistochemical staining

    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Journal: Pharmaceuticals

    Article Title: Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination

    doi: 10.3390/ph14111156

    Figure Lengend Snippet: Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Article Snippet: The following primary antibodies were used: for myelin proteolipid protein (PLP) (mouse monoclonal IgG2a, 1:500, Serotec) and myelin basic protein (MBP) (mouse monoclonal IgG2b, 1:500, Covance), for activated microglia Mac-3 (rat IgG, 1:500, BD Pharmingen) and the lectin ricinus communis agglutinin 1 (RCA-1) (1:1000, biotinylated, Vector Laboratories), for astrocytes glial fibrillary acidic protein (GFAP) (mouse IgG, 1:200, Millipore), for oligodendrocytes Nogo-A (rabbit IgG, 1:750, Millipore) and anti-adenomatus polyposis coli (APC; mouse IgG; 1:200).

    Techniques:

    Journal: STAR Protocols

    Article Title: Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

    doi: 10.1016/j.xpro.2021.100678

    Figure Lengend Snippet:

    Article Snippet: Fluorescein Ricinus communis agglutinin-1 lectin , Vector Labs , FL-1081.

    Techniques: Recombinant, Staining, Plasmid Preparation, Software, Phagocytosis Assay, Microscopy

    NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis Agglutinin-1); Magnification: 20X, scale bar: 100 µm.

    Journal: Scientific Reports

    Article Title: Differential Expression Profile of NLRs and AIM2 in Glioma and Implications for NLRP12 in Glioblastoma

    doi: 10.1038/s41598-019-44854-4

    Figure Lengend Snippet: NLRP12 inhibition regulates cellular proliferation. ( a ) NLRP12 expression was observed in glioma (LN18) and microglial (BV2) cells using immunofluorescence (magnification: 40X, scale bar: 50 μm). ( b ) Colony formation assay shows increased cellular proliferation in the NLRP12si treated microglia. Magnification: 40X. ( c , d ) Quantification of both BV2 and LN18 cells per colony. ( e ) Effect of LN18 (NLRP12 siRNA treated) cell conditioned medium (CM) on BV2 cell migration was assessed using migration assay. 8 image sections per sample were quantified for migration analysis. Student’s t-test and one-way AVOVA were performed to find the statistical significance of colony formation and migration assay respectively (p-value; *<0.05, **<0.005). The error bar indicates standard error of mean. ( f ) NLRP12 expression in human normal brain tissue and glioma sections, using anti-NLRP12 antibody (N = 7). Nuclei were stained blue with DAPI and microglia were stained green with FITC tagged lectin RCA (Ricinus Communis Agglutinin-1); Magnification: 20X, scale bar: 100 µm.

    Article Snippet: For the detection of microglia, tissues were stained with Ricinus communis agglutinin-1 (RCA-1) lectin (Vector labs, FL-1081) .

    Techniques: Inhibition, Expressing, Immunofluorescence, Colony Assay, Migration, Staining