lc pcr master mix  (Roche)


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    Structured Review

    Roche lc pcr master mix
    Detection of HSV <t>DNA</t> from clinical samples by LightCycler <t>PCR.</t>
    Lc Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc pcr master mix/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc pcr master mix - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture"

    Article Title: Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-2-12

    Detection of HSV DNA from clinical samples by LightCycler PCR.
    Figure Legend Snippet: Detection of HSV DNA from clinical samples by LightCycler PCR.

    Techniques Used: Polymerase Chain Reaction

    2) Product Images from "Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV"

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.44.2.423-432.2006

    Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)
    Figure Legend Snippet: Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)

    Techniques Used: Quantitative RT-PCR

    HSV-2 DNA quantification by real-time PCR.
    Figure Legend Snippet: HSV-2 DNA quantification by real-time PCR.

    Techniques Used: Real-time Polymerase Chain Reaction

    3) Product Images from "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity"

    Article Title: Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity

    Journal: AIDS Research and Therapy

    doi: 10.1186/1742-6405-5-10

    Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.
    Figure Legend Snippet: Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.

    Techniques Used: Activity Assay, Derivative Assay, Incubation, Cell Culture, Real-time Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV
    Article Snippet: .. The LC PCR master mix contained 1× Fast-Start Taq DNA polymerase reaction buffer (Roche Diagnostics), 3 mM MgCl2 , 0.5 μM concentrations of each primer, 0.2 μM HSV-2 FLU, and 0.4 μM HSV-2 LCR. .. Cycling conditions were as follows: initial denaturation and Fast-Start Taq DNA polymerase activation at 95°C for 10 min, 45 cycles of denaturation at 95°C for 5 s, annealing at 58°C for 10 s (with fluorescence acquisition at the end of each annealing stage), and extension at 72°C for 12 s. After amplification was complete, melting curve analysis was performed as follows: denaturation at 95°C for 20 s and annealing at 45°C for 30 s followed by a gradual increase in temperature (transition rate of 0.1°C/s) to 85°C with continuous fluorescence acquisition.

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    Roche lc pcr master mix
    Detection of HSV <t>DNA</t> from clinical samples by LightCycler <t>PCR.</t>
    Lc Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc pcr master mix/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc pcr master mix - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    86
    Roche lc fast start dna master plus sybr green pcr mix
    Detection of HSV <t>DNA</t> from clinical samples by LightCycler <t>PCR.</t>
    Lc Fast Start Dna Master Plus Sybr Green Pcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc fast start dna master plus sybr green pcr mix/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc fast start dna master plus sybr green pcr mix - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Detection of HSV DNA from clinical samples by LightCycler PCR.

    Journal: BMC Microbiology

    Article Title: Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture

    doi: 10.1186/1471-2180-2-12

    Figure Lengend Snippet: Detection of HSV DNA from clinical samples by LightCycler PCR.

    Article Snippet: The LC-PCR master mix contained the following: 1× FastStart Taq DNA polymerase reaction buffer (Roche Molecular Diagnostics) which included a dNTP mix (containing dUTP instead of dTTP), 3 mM MgCl2 , 0.5 μM of each primer, 0.2 μM HSV-2 FLU and 0.4 μM HSV-2 LCR.

    Techniques: Polymerase Chain Reaction

    Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)

    Journal: Journal of Clinical Microbiology

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV

    doi: 10.1128/JCM.44.2.423-432.2006

    Figure Lengend Snippet: Differences between the mean loads measured by the branched DNA assay and real-time RT-PCR by using the Bland-Altman plot analysis. The mean difference (bias) is indicated by a solid line, and the 95% confidence interval (±2 standard deviations)

    Article Snippet: The LC PCR master mix contained 1× Fast-Start Taq DNA polymerase reaction buffer (Roche Diagnostics), 3 mM MgCl2 , 0.5 μM concentrations of each primer, 0.2 μM HSV-2 FLU, and 0.4 μM HSV-2 LCR.

    Techniques: Quantitative RT-PCR

    HSV-2 DNA quantification by real-time PCR.

    Journal: Journal of Clinical Microbiology

    Article Title: Real-Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV

    doi: 10.1128/JCM.44.2.423-432.2006

    Figure Lengend Snippet: HSV-2 DNA quantification by real-time PCR.

    Article Snippet: The LC PCR master mix contained 1× Fast-Start Taq DNA polymerase reaction buffer (Roche Diagnostics), 3 mM MgCl2 , 0.5 μM concentrations of each primer, 0.2 μM HSV-2 FLU, and 0.4 μM HSV-2 LCR.

    Techniques: Real-time Polymerase Chain Reaction

    Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.

    Journal: AIDS Research and Therapy

    Article Title: Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity

    doi: 10.1186/1742-6405-5-10

    Figure Lengend Snippet: Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells. Monocyte-derived macrophages (A), monocyte-derived dendritic cells (B) and T cells (C) were incubated with R5 or X4 viruses in the presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for 3 hours at 37°C. Cells were then washed and cultured in fresh medium for 3 days. DNA was extracted and the viral DNA was quantified by real time PCR. Means are representative of 3 independent experiments and assays were performed in triplicates. *, p ≤ 0.05; **, p ≤ 0.01 between untreated and treated cells.

    Article Snippet: The LC-PCR master mix contained 1 × Fast-Start Taq DNA polymerase reaction buffer (Roche Applied Science), 3 mM MgCl2 , 0.3 μM of each primer and probe.

    Techniques: Activity Assay, Derivative Assay, Incubation, Cell Culture, Real-time Polymerase Chain Reaction