lc msms analysis system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lc msms analysis system
    Lc Msms Analysis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc msms analysis system/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc msms analysis system - by Bioz Stars, 2020-07
    85/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Effect of Curcumin Supplement in Summer Diet on Blood Metabolites, Antioxidant Status, Immune Response, and Testicular Gene Expression in Hu Sheep
    Article Snippet: .. Real-time PCR was performed using the QuantStudio® 5 Real-Time PCR Design and Analysis System (Applied Biosystems, USA) with a SYBR® Premix Ex Taq™ Kit (TaKaRa Biotechnology Co. Ltd., Dalian, China). .. The reactions include TB Green Premix Ex Taq (10 μL), PCR Primers (0.8 μL), ROX Reference Dye II (0.4 μL), DNA template (2 μL) and sterilized water with a final volume of 20 μL, and the cycling conditions were as follows: 95 °C for 30 s (Hold stage), followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s (PCR stage), then 95 °C for 15 s, 60 °C for one min, and 95 °C for 15 s (Melt curve stage).

    Injection:

    Article Title: Identification of a high-affinity binding site involved in the transport of endocannabinoids
    Article Snippet: .. Anandamide in cerebellar tissue samples from male Sprague-Dawley rats (Harlan Labs, Indianapolis; weight, 200-250 g) ( n = 4-8 per group) was measured 90 min after i.p. drug injection by using a liquid chromatography/tandem MS (LC/MSMS) analysis system (triple quadrapole mass spectral detector; API 3000, Applied Biosystems) with fast gradient elution and a C18 column. ..

    Article Title: The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats
    Article Snippet: .. Previously, the analysis system was preconditioned at 15% A and 85% C for 15 min. Each sample (25 µL) was injected with an ICS AS-DV 50 auto sampler (Dionex, Sunnyvale, CA, USA). ..

    Imaging:

    Article Title: LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
    Article Snippet: .. While this protocol offers specifications for InCucyte live imaging and analysis system, the protocol can be adapted for alternative imaging systems such as Cellomics. ..

    Mass Spectrometry:

    Article Title: Identification of a high-affinity binding site involved in the transport of endocannabinoids
    Article Snippet: .. Anandamide in cerebellar tissue samples from male Sprague-Dawley rats (Harlan Labs, Indianapolis; weight, 200-250 g) ( n = 4-8 per group) was measured 90 min after i.p. drug injection by using a liquid chromatography/tandem MS (LC/MSMS) analysis system (triple quadrapole mass spectral detector; API 3000, Applied Biosystems) with fast gradient elution and a C18 column. ..

    Chromatography:

    Article Title: Identification of a high-affinity binding site involved in the transport of endocannabinoids
    Article Snippet: .. Anandamide in cerebellar tissue samples from male Sprague-Dawley rats (Harlan Labs, Indianapolis; weight, 200-250 g) ( n = 4-8 per group) was measured 90 min after i.p. drug injection by using a liquid chromatography/tandem MS (LC/MSMS) analysis system (triple quadrapole mass spectral detector; API 3000, Applied Biosystems) with fast gradient elution and a C18 column. ..

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    Thermo Fisher lc msms analysis
    <t>Orbitrap</t> <t>MSMS</t> spectrum of peptide PPPPP PPPPP PPP isolated from boiled FBS AChE by HPLC. The doubly-charged parent ion has a mass of 640.85 m/z. Amino acid sequences are marked to show singly-charged y-ion series, singly-charge b-ion series, a doubly-charged
    Lc Msms Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc msms analysis/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lc msms analysis - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Thermo Fisher liquid chromatography tandem mass spectrometry lc msms
    Reduced <t>MS‐MSC</t> secretion of SOD1 and GSTP1 in association with duration of progressive phase of MS. Analysis of the MSC secretome was undertaken using <t>LC‐MSMS</t> (C‐MSC n = 4; MS‐MSC n = 6) and ELISA (C‐MSC n = 5; MS‐MSC n = 11). (A): When the difference in age between the cohorts was accounted for, an independent, statistically significant effect of the presence of MS was seen with reduced secretion of SOD1 (regression analysis, #, p = .042, CI −0.093 to 3.9) as measured by LC‐MSMS. (B): Reduced secretion of SOD1 by MS‐MSC as determined by ELISA (regression analysis, ###, p
    Liquid Chromatography Tandem Mass Spectrometry Lc Msms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry lc msms/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry lc msms - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Orbitrap MSMS spectrum of peptide PPPPP PPPPP PPP isolated from boiled FBS AChE by HPLC. The doubly-charged parent ion has a mass of 640.85 m/z. Amino acid sequences are marked to show singly-charged y-ion series, singly-charge b-ion series, a doubly-charged

    Journal: Biochimica et biophysica acta

    Article Title: Polyproline Tetramer Organizing Peptides in Fetal Bovine Serum Acetylcholinesterase

    doi: 10.1016/j.bbapap.2013.01.009

    Figure Lengend Snippet: Orbitrap MSMS spectrum of peptide PPPPP PPPPP PPP isolated from boiled FBS AChE by HPLC. The doubly-charged parent ion has a mass of 640.85 m/z. Amino acid sequences are marked to show singly-charged y-ion series, singly-charge b-ion series, a doubly-charged

    Article Snippet: LC/MSMS analysis was performed on an LTQ Orbitrap quadrupole mass spectrometer (Thermo Scientific a part of Thermo Fisher Scientific, Rockland, IL) using electrospray ionization.

    Techniques: Isolation, High Performance Liquid Chromatography

    Orbitrap MSMS spectrum of peptide SPPPP PPPPP PP isolated from boiled horse BChE by HPLC. The parent ion mass was doubly charged m/z 587.3 [M+2H] 2+ Amino acid sequences are annotated to show a singly-charged y-ion series, a singly-charge b-ion series and a doubly-charged b-ion series (marked with +2). The y-axis is labeled in abundance, relative to the most intense peak in the spectrum.

    Journal: The FEBS journal

    Article Title: The Proline Rich Tetramerization Peptides in Equine Serum Butyrylcholinesterase

    doi: 10.1111/j.1742-4658.2012.08744.x

    Figure Lengend Snippet: Orbitrap MSMS spectrum of peptide SPPPP PPPPP PP isolated from boiled horse BChE by HPLC. The parent ion mass was doubly charged m/z 587.3 [M+2H] 2+ Amino acid sequences are annotated to show a singly-charged y-ion series, a singly-charge b-ion series and a doubly-charged b-ion series (marked with +2). The y-axis is labeled in abundance, relative to the most intense peak in the spectrum.

    Article Snippet: LC/MSMS analysis was performed on an LTQ Orbitrap quadrupole mass spectrometer (Thermo Scientific a part of Thermo Fisher Scientific, Rockland, IL, U.S.A.) using electrospray ionization.

    Techniques: Isolation, High Performance Liquid Chromatography, Labeling

    Reduced MS‐MSC secretion of SOD1 and GSTP1 in association with duration of progressive phase of MS. Analysis of the MSC secretome was undertaken using LC‐MSMS (C‐MSC n = 4; MS‐MSC n = 6) and ELISA (C‐MSC n = 5; MS‐MSC n = 11). (A): When the difference in age between the cohorts was accounted for, an independent, statistically significant effect of the presence of MS was seen with reduced secretion of SOD1 (regression analysis, #, p = .042, CI −0.093 to 3.9) as measured by LC‐MSMS. (B): Reduced secretion of SOD1 by MS‐MSC as determined by ELISA (regression analysis, ###, p

    Journal: Stem Cells Translational Medicine

    Article Title: Dysregulation of Mesenchymal Stromal Cell Antioxidant Responses in Progressive Multiple Sclerosis

    doi: 10.1002/sctm.18-0045

    Figure Lengend Snippet: Reduced MS‐MSC secretion of SOD1 and GSTP1 in association with duration of progressive phase of MS. Analysis of the MSC secretome was undertaken using LC‐MSMS (C‐MSC n = 4; MS‐MSC n = 6) and ELISA (C‐MSC n = 5; MS‐MSC n = 11). (A): When the difference in age between the cohorts was accounted for, an independent, statistically significant effect of the presence of MS was seen with reduced secretion of SOD1 (regression analysis, #, p = .042, CI −0.093 to 3.9) as measured by LC‐MSMS. (B): Reduced secretion of SOD1 by MS‐MSC as determined by ELISA (regression analysis, ###, p

    Article Snippet: Liquid chromatography‐tandem mass spectrometry (LC‐MSMS) of C‐MSC and MS‐MSC conditioned medium was performed by the University of Bristol Proteomics Facility using a previously described protocol for tandem mass tagging (Thermo Fisher Scientific USA) coupled to liquid chromatography‐mass spectrometry .

    Techniques: Mass Spectrometry, Enzyme-linked Immunosorbent Assay