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Fisher Scientific lc ms grade
Lc Ms Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lc ms grade/product/Fisher Scientific
Average 92 stars, based on 60 article reviews
Price from $9.99 to $1999.99
lc ms grade - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Adapted MS/MSALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species
Article Snippet: .. All solvents were HPLC or LC/MS grade and were purchased from Fisher Scientific (Waltham, MA) or VWR International (Radnor, PA). ..

Article Title: Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids
Article Snippet: .. Butylated hydroxytoluene 2,6-di- tert -butyl- p -cresol (BHT) and all solvents, including MeOH, acetonitrile (ACN), isopropanol (IPA), chloroform (CHCl3 ), and water (H2 O), of LC-MS grade and high performance liquid chromatography (HPLC) grade were purchased from Fisher Scientific (Pittsburgh, PA). ..

Indirect Immunoperoxidase Assay:

Article Title: Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids
Article Snippet: .. Butylated hydroxytoluene 2,6-di- tert -butyl- p -cresol (BHT) and all solvents, including MeOH, acetonitrile (ACN), isopropanol (IPA), chloroform (CHCl3 ), and water (H2 O), of LC-MS grade and high performance liquid chromatography (HPLC) grade were purchased from Fisher Scientific (Pittsburgh, PA). ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: Supplementation of L-Alanyl-L-Glutamine and Fish Oil Improves Body Composition and Quality of Life in Patients With Chronic Heart Failure
Article Snippet: .. All solvents for sample extraction and LC/MS were LC/MS grade (or LC grade when LC/MS grade was not available) and were purchased from Fisher Scientific (Pittsburgh, PA, USA). .. Standards were purchased from Avanti Polar Lipid, Inc. (Alabaster, AL, USA).

Article Title: KCNQ1, KCNE2, and Na+-Coupled Solute Transporters Form Reciprocally Regulating Complexes that Affect Neuronal Excitability
Article Snippet: .. LC-MS grade of acetonitrile, methanol and sonic dismembrator model 100 were purchased from Fisher Scientific. .. The internal standard, [2 H6 ]-myo-inositol(MID6), was purchased from Cambridge Isotope Laboratories, Inc.(MA, USA).

Article Title: Extraction parameters for metabolomics from cell extracts
Article Snippet: .. All solvents used were LC-MS grade and purchased from Fisher Scientific. ..

Article Title: Adapted MS/MSALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species
Article Snippet: .. All solvents were HPLC or LC/MS grade and were purchased from Fisher Scientific (Waltham, MA) or VWR International (Radnor, PA). ..

Article Title: UPLC-ESI-QTOF-MS2-Based Identification and Antioxidant Activity Assessment of Phenolic Compounds from Red Corn Cob (Zea mays L.)
Article Snippet: .. Acetonitrile, methanol, formic acid, and water were of LC-MS grade and were purchased from Fisher Scientific Chemicals (Fair Lawn, NJ, EE.UU.). ..

Article Title: Lignans in Spirits: Chemical Diversity, Quantification, and Sensory Impact of (±)-Lyoniresinol
Article Snippet: .. Acetonitrile (ACN) and water used for chromatographic separation were LC-MS grade and were purchased from Fisher Chemical (Illkirch, France). .. Lignans were isolated as previously described by Marchal et al. [ ].

Article Title: Mapping of Phospholipids by MALDI Imaging (MALDI-MSI): Realities and Expectations.
Article Snippet: .. Methanol, trifluoroacetic acid (TFA), triethylamine, acetic acid were all LC-MS grade from Fisher Scientific (Pittsburgh, PA, USA). .. Water for LC use was LC-MS grade purchased from Fisher Scientific (Pittsburgh, PA, USA); water for other uses was purified by a milliQ system (EMD Millipore, Billerica, MA, USA).

Article Title: Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids
Article Snippet: .. Butylated hydroxytoluene 2,6-di- tert -butyl- p -cresol (BHT) and all solvents, including MeOH, acetonitrile (ACN), isopropanol (IPA), chloroform (CHCl3 ), and water (H2 O), of LC-MS grade and high performance liquid chromatography (HPLC) grade were purchased from Fisher Scientific (Pittsburgh, PA). ..

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  • 92
    Fisher Scientific lc ms grade acetonitrile mecn
    Synthesis of 1,4-isoquinolines 15 and 17 a a Reagents: (a) PBr 5 , 140 °C, 89% yield; (b) CuI, N,N’ -dimethylethylenediamine, K 2 CO 3 , <t>MeCN,</t> 70 °C, 60% yield; (c) RBr, K 2 CO 3 , DMF, rt, 77% yield; (d) NaOH, <t>MeOH,</t> reflux, 36% yield. rt: room temperature
    Lc Ms Grade Acetonitrile Mecn, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms grade acetonitrile mecn/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc ms grade acetonitrile mecn - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Fisher Scientific lc ms grade ammonium acetate
    Trehalose calibration curves from (a) the enzymatic trehalose assay, (b) the HPLC-RID-based assay, and (c) the <t>LC–MS/MS-based</t> assay. (a) Trehalose calibration curve for the enzymatic trehalose assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (25–1000 μM) were subjected to the enzymatic trehalose assay (sample volume = 20 μL, Trehalose Assay Kit; Megazyme International, Ireland), and the absorbances were measured. Error bars represent the standard deviation ( n = 3). (b) Trehalose calibration curve for the HPLC-RID assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (1–100 mM) were injected onto the HPLC in triplicate (mobile phase = 23:77 water/acetonitrile, injection volume = 50 μL), and the microrefractive index units (μRIU) for trehalose were measured. Error bars represent the standard deviation ( n = 3). (c) Trehalose calibration curve for the LC–MS/MS assay using 13 C 12 -trehalose as an internal standard is best fit by a single polynomial. Standards containing varying concentrations of trehalose (0.1–100 μM) with 5 μM 13 C 12 -trehalose as an internal standard were injected onto the LC–MS/MS in quadruplicate (mobile phase = 20:80 2 mM <t>ammonium</t> <t>acetate</t> in water/2 mM ammonium acetate in acetonitrile, injection volume = 40 μL), and the relative responses for trehalose were calculated. Error bars represent the standard deviation ( n = 4).
    Lc Ms Grade Ammonium Acetate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms grade ammonium acetate/product/Fisher Scientific
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc ms grade ammonium acetate - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    91
    Fisher Scientific lc ms grade methanol meoh
    Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 <t>O/MeOH/ACN/HAc</t> gradient.
    Lc Ms Grade Methanol Meoh, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms grade methanol meoh/product/Fisher Scientific
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc ms grade methanol meoh - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    92
    Fisher Scientific lc ms grade acetonitrile
    Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using <t>LC-MS</t> with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an <t>acetonitrile</t> gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .
    Lc Ms Grade Acetonitrile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc ms grade acetonitrile/product/Fisher Scientific
    Average 92 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    lc ms grade acetonitrile - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Synthesis of 1,4-isoquinolines 15 and 17 a a Reagents: (a) PBr 5 , 140 °C, 89% yield; (b) CuI, N,N’ -dimethylethylenediamine, K 2 CO 3 , MeCN, 70 °C, 60% yield; (c) RBr, K 2 CO 3 , DMF, rt, 77% yield; (d) NaOH, MeOH, reflux, 36% yield. rt: room temperature

    Journal: Journal of medicinal chemistry

    Article Title: Replacement of a Naphthalene Scaffold in Kelch-like ECH-Associated Protein 1 (KEAP1)/ Nuclear factor (erythroid-derived 2)-like 2 (NRF2) Inhibitors

    doi: 10.1021/acs.jmedchem.8b01133

    Figure Lengend Snippet: Synthesis of 1,4-isoquinolines 15 and 17 a a Reagents: (a) PBr 5 , 140 °C, 89% yield; (b) CuI, N,N’ -dimethylethylenediamine, K 2 CO 3 , MeCN, 70 °C, 60% yield; (c) RBr, K 2 CO 3 , DMF, rt, 77% yield; (d) NaOH, MeOH, reflux, 36% yield. rt: room temperature

    Article Snippet: LC-MS grade acetonitrile (MeCN), methanol (MeOH) and formic acid, biology grade DMSO were purchased from Fisher Scientific (Pittsburgh, PA).

    Techniques: Reflux

    Trehalose calibration curves from (a) the enzymatic trehalose assay, (b) the HPLC-RID-based assay, and (c) the LC–MS/MS-based assay. (a) Trehalose calibration curve for the enzymatic trehalose assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (25–1000 μM) were subjected to the enzymatic trehalose assay (sample volume = 20 μL, Trehalose Assay Kit; Megazyme International, Ireland), and the absorbances were measured. Error bars represent the standard deviation ( n = 3). (b) Trehalose calibration curve for the HPLC-RID assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (1–100 mM) were injected onto the HPLC in triplicate (mobile phase = 23:77 water/acetonitrile, injection volume = 50 μL), and the microrefractive index units (μRIU) for trehalose were measured. Error bars represent the standard deviation ( n = 3). (c) Trehalose calibration curve for the LC–MS/MS assay using 13 C 12 -trehalose as an internal standard is best fit by a single polynomial. Standards containing varying concentrations of trehalose (0.1–100 μM) with 5 μM 13 C 12 -trehalose as an internal standard were injected onto the LC–MS/MS in quadruplicate (mobile phase = 20:80 2 mM ammonium acetate in water/2 mM ammonium acetate in acetonitrile, injection volume = 40 μL), and the relative responses for trehalose were calculated. Error bars represent the standard deviation ( n = 4).

    Journal: ACS Omega

    Article Title: Quantification of the Disaccharide Trehalose from Biological Samples: A Comparison of Analytical Methods

    doi: 10.1021/acsomega.7b01158

    Figure Lengend Snippet: Trehalose calibration curves from (a) the enzymatic trehalose assay, (b) the HPLC-RID-based assay, and (c) the LC–MS/MS-based assay. (a) Trehalose calibration curve for the enzymatic trehalose assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (25–1000 μM) were subjected to the enzymatic trehalose assay (sample volume = 20 μL, Trehalose Assay Kit; Megazyme International, Ireland), and the absorbances were measured. Error bars represent the standard deviation ( n = 3). (b) Trehalose calibration curve for the HPLC-RID assay is best fit by a linear curve. Standards containing varying concentrations of trehalose (1–100 mM) were injected onto the HPLC in triplicate (mobile phase = 23:77 water/acetonitrile, injection volume = 50 μL), and the microrefractive index units (μRIU) for trehalose were measured. Error bars represent the standard deviation ( n = 3). (c) Trehalose calibration curve for the LC–MS/MS assay using 13 C 12 -trehalose as an internal standard is best fit by a single polynomial. Standards containing varying concentrations of trehalose (0.1–100 μM) with 5 μM 13 C 12 -trehalose as an internal standard were injected onto the LC–MS/MS in quadruplicate (mobile phase = 20:80 2 mM ammonium acetate in water/2 mM ammonium acetate in acetonitrile, injection volume = 40 μL), and the relative responses for trehalose were calculated. Error bars represent the standard deviation ( n = 4).

    Article Snippet: Materials d -(+)-Trehalose dihydrate ( > 99%) and LC–MS-grade ammonium acetate ( > 99%) were purchased from Fisher Scientific.

    Techniques: High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Standard Deviation, Injection

    Specificity of assays for trehalose detection. (a) Corrected absorbances for lactose, maltose, sucrose, and trehalose for the enzymatic trehalose assay. All disaccharide concentrations were 1 mM, and the sample volume was 20 μL. For all corrected absorbance values, n = 3. (b) RI chromatogram showing the separation of trehalose (retention time = 33.2 min) from three other disaccharides, sucrose (retention time = 19.3 min), maltose (retention time = 25.5 min), and lactose (retention time = 30.0 min) for the HPLC-RID-based assay. Separation was achieved using a Waters high-performance carbohydrate column (4.6 × 250 mm 2 , 4 μm) held at 35 °C. The mobile phase was composed of 16:84 water/acetonitrile with an isocratic elution over 40 min using a flow rate of 1.4 mL/min. All disaccharide concentrations were 20 mM, and the injection volume was 50 μL. (c) SRM chromatogram showing the separation of trehalose (retention time = 30.3 min) from three other disaccharides, sucrose (retention time = 18.2 min), maltose (retention time = 23.8 min), and lactose (not detected by this method) for the LC–MS/MS-based assay. Separation was achieved using a Waters high-performance carbohydrate column (4.6 × 250 mm 2 , 4 μm) held at 35 °C. The mobile phase was composed of 16:84 2 mM ammonium acetate in water/2 mM ammonium acetate in acetonitrile with an isocratic elution over 35 min using a flow rate of 1.4 mL/min. All disaccharide concentrations were 25 μM, and the injection volume was 40 μL.

    Journal: ACS Omega

    Article Title: Quantification of the Disaccharide Trehalose from Biological Samples: A Comparison of Analytical Methods

    doi: 10.1021/acsomega.7b01158

    Figure Lengend Snippet: Specificity of assays for trehalose detection. (a) Corrected absorbances for lactose, maltose, sucrose, and trehalose for the enzymatic trehalose assay. All disaccharide concentrations were 1 mM, and the sample volume was 20 μL. For all corrected absorbance values, n = 3. (b) RI chromatogram showing the separation of trehalose (retention time = 33.2 min) from three other disaccharides, sucrose (retention time = 19.3 min), maltose (retention time = 25.5 min), and lactose (retention time = 30.0 min) for the HPLC-RID-based assay. Separation was achieved using a Waters high-performance carbohydrate column (4.6 × 250 mm 2 , 4 μm) held at 35 °C. The mobile phase was composed of 16:84 water/acetonitrile with an isocratic elution over 40 min using a flow rate of 1.4 mL/min. All disaccharide concentrations were 20 mM, and the injection volume was 50 μL. (c) SRM chromatogram showing the separation of trehalose (retention time = 30.3 min) from three other disaccharides, sucrose (retention time = 18.2 min), maltose (retention time = 23.8 min), and lactose (not detected by this method) for the LC–MS/MS-based assay. Separation was achieved using a Waters high-performance carbohydrate column (4.6 × 250 mm 2 , 4 μm) held at 35 °C. The mobile phase was composed of 16:84 2 mM ammonium acetate in water/2 mM ammonium acetate in acetonitrile with an isocratic elution over 35 min using a flow rate of 1.4 mL/min. All disaccharide concentrations were 25 μM, and the injection volume was 40 μL.

    Article Snippet: Materials d -(+)-Trehalose dihydrate ( > 99%) and LC–MS-grade ammonium acetate ( > 99%) were purchased from Fisher Scientific.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 O/MeOH/ACN/HAc gradient.

    Journal: Frontiers in Pharmacology

    Article Title: Development of an Optimized LC-MS Method for the Detection of Specialized Pro-Resolving Mediators in Biological Samples

    doi: 10.3389/fphar.2019.00169

    Figure Lengend Snippet: Chromatographic separation of (A) 18 SPMs (100 nM each) and (B) 5 deuterated internal standards (20 nM) covered by the method including (C) DHA derived D-series resolvins, (D) EPA derived E-series resolvins, (E) EPA and ARA derived lipoxins, DHA derived (F) protectins, and (G) maresins. Separation was carried out on an RP-18 column (2.1 × 150 mm, particle size 1.8 μm, pore size 9.5 nm) with a H 2 O/MeOH/ACN/HAc gradient.

    Article Snippet: Acetonitrile (ACN), LC-MS grade methanol (MeOH) and acetic acid were obtained from Fisher Scientific (Schwerte, Germany).

    Techniques: Derivative Assay, Acetylene Reduction Assay, HAC Assay

    Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using LC-MS with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using LC-MS with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Article Snippet: For HPLC or LC-MS, LC-MS grade acetonitrile, water and formic acid were purchased from Fisher Scientific (Hampton, NH, USA), and LC-MS grade methanol was purchased from Honeywell international Inc. (Charlotte, NC, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

    Detection of 25(OH)D3 in honey. After a pre-purification step using a C18 column with an acetonitrile gradient, the fraction was analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 25(OH)D3 in honey. After a pre-purification step using a C18 column with an acetonitrile gradient, the fraction was analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Article Snippet: For HPLC or LC-MS, LC-MS grade acetonitrile, water and formic acid were purchased from Fisher Scientific (Hampton, NH, USA), and LC-MS grade methanol was purchased from Honeywell international Inc. (Charlotte, NC, USA).

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

    Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Article Snippet: For HPLC or LC-MS, LC-MS grade acetonitrile, water and formic acid were purchased from Fisher Scientific (Hampton, NH, USA), and LC-MS grade methanol was purchased from Honeywell international Inc. (Charlotte, NC, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification