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SCIEX lc esi ms ms approach
IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS <t>ceramides</t> with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry <t>(LC-ESI-MS/MS)</t> and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
Lc Esi Ms Ms Approach, supplied by SCIEX, used in various techniques. Bioz Stars score: 92/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Lipid abnormalities in atopic skin are driven by type 2 cytokines"

Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

Journal: JCI Insight

doi: 10.1172/jci.insight.98006

IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
Figure Legend Snippet: IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P

Techniques Used: Mouse Assay, Liquid Chromatography, Mass Spectrometry, Whisker Assay

The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.
Figure Legend Snippet: The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.

Techniques Used: In Vitro, Liquid Chromatography, Mass Spectrometry, Two Tailed Test

The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P
Figure Legend Snippet: The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P

Techniques Used: Liquid Chromatography, Mass Spectrometry, Whisker Assay

2) Product Images from "Oxidized LDL signals through Rho-GTPase to induce endothelial cell stiffening and promote capillary formation [S]"

Article Title: Oxidized LDL signals through Rho-GTPase to induce endothelial cell stiffening and promote capillary formation [S]

Journal: Journal of Lipid Research

doi: 10.1194/jlr.M062539

The 7KC in oxLDL induces RhoA activity. A: Representative multiple reaction monitoring profiles of cholesterol and oxysterols in control LDL (left) and copper-oxidized LDL (right) preparations analyzed as bis -acetate derivatives. Note that signal intensities
Figure Legend Snippet: The 7KC in oxLDL induces RhoA activity. A: Representative multiple reaction monitoring profiles of cholesterol and oxysterols in control LDL (left) and copper-oxidized LDL (right) preparations analyzed as bis -acetate derivatives. Note that signal intensities

Techniques Used: Activity Assay

3) Product Images from "Lipid abnormalities in atopic skin are driven by type 2 cytokines"

Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

Journal: JCI Insight

doi: 10.1172/jci.insight.98006

IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
Figure Legend Snippet: IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P

Techniques Used: Mouse Assay, Liquid Chromatography, Mass Spectrometry, Whisker Assay

The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.
Figure Legend Snippet: The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.

Techniques Used: In Vitro, Liquid Chromatography, Mass Spectrometry, Two Tailed Test

The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P
Figure Legend Snippet: The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P

Techniques Used: Liquid Chromatography, Mass Spectrometry, Whisker Assay

Related Articles

High Performance Liquid Chromatography:

Article Title: Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase
Article Snippet: .. Bands migrating with the expected molecular mass of HA–NUAK1 were excised from the gel, digested with trypsin and subjected to HPLC-MS/MS on an ABSciex QTrap 4000 mass spectrometer using precursor ion scanning for −79 Da [ ]. .. XIC (extracted ion chromatogram analysis; where the total signal intensity of the phosphopeptide was plotted on the y -axis and retention time was plotted on the x -axis) of the Ser476 - and Ser480 -containing phosphopeptide (R.ESGYYSSPER.S+1P) was obtained manually using Analyst software (ABSciex).

Mass Spectrometry:

Article Title: GM-CSF driven myeloid cells in adipose tissue link weight gain and insulin resistance via formation of 2-aminoadipate
Article Snippet: .. Derivatized amino acids were analyzed using a 4000 QTRAP hybrid/triple quadrupole linear ion trap mass spectrometer (SCIEX, Foster City, CA) with electrospray ionization (ESI) in positive mode. .. The mass spectrometer was interfaced to a Shimadzu (Columbia, MD) SIL-20AC XR auto-sampler followed by 2 LC-20AD XR LC pumps.

Article Title: Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase
Article Snippet: .. Bands migrating with the expected molecular mass of HA–NUAK1 were excised from the gel, digested with trypsin and subjected to HPLC-MS/MS on an ABSciex QTrap 4000 mass spectrometer using precursor ion scanning for −79 Da [ ]. .. XIC (extracted ion chromatogram analysis; where the total signal intensity of the phosphopeptide was plotted on the y -axis and retention time was plotted on the x -axis) of the Ser476 - and Ser480 -containing phosphopeptide (R.ESGYYSSPER.S+1P) was obtained manually using Analyst software (ABSciex).

Article Title: Rapid Verification of Candidate Serological Biomarkers Using Gel-based, Label-free Multiple Reaction Monitoring
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Tandem Mass Spectroscopy:

Article Title: Deciphering lipid structures based on platform-independent decision rule sets
Article Snippet: .. Decision rule sets were tested on the following MS/MS platforms: 4000 QTRAP and QTRAP 6500 from AB Sciex; G6550A QTOF from Agilent Technologies; Orbitrap Elite, Orbitrap Velos Pro in CID and HCD mode, and Q Exactive from Thermo Fisher Scientific; SYNAPT G1 HDMS QTOF from Waters. .. The primary raw data format is mzXML ; however, the software allows for direct processing of vendor formats from AB Sciex, Agilent Technologies, Bruker Daltonics and Thermo Fisher Scientific by an integrated version of msConvert , as we obtained permission for redistribution of vendor-provided libraries from respective mass spectrometer manufacturers.

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    SCIEX lc esi ms ms approach
    IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS <t>ceramides</t> with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry <t>(LC-ESI-MS/MS)</t> and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
    Lc Esi Ms Ms Approach, supplied by SCIEX, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc esi ms ms approach/product/SCIEX
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc esi ms ms approach - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    SCIEX 6500 qtrap mass spectrometer interfaces
    IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS <t>ceramides</t> with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry <t>(LC-ESI-MS/MS)</t> and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
    6500 Qtrap Mass Spectrometer Interfaces, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6500 qtrap mass spectrometer interfaces/product/SCIEX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6500 qtrap mass spectrometer interfaces - by Bioz Stars, 2020-09
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    SCIEX esi lc ms ms approach
    IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS <t>ceramides</t> with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry <t>(LC-ESI-MS/MS)</t> and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P
    Esi Lc Ms Ms Approach, supplied by SCIEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi lc ms ms approach/product/SCIEX
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    esi lc ms ms approach - by Bioz Stars, 2020-09
    90/100 stars
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    IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P

    Journal: JCI Insight

    Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

    doi: 10.1172/jci.insight.98006

    Figure Lengend Snippet: IL-13 driven lesion development in mouse skin profoundly affects stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in NS ceramides with C18 sphingosine ( A ), sphingomyelins ( B ), and lysophosphatidylcholines ( C ) in stratum corneum from nonlesional and lesional skin of IL-13 Tg mice and their littermate controls. IL-13 Tg mice spontaneously develop skin lesions. Tape strips were collected from both lesional and nonlesional areas of mouse skin. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. * P

    Article Snippet: Ceramides, LPC, and SM were identified and quantified using the targeted LC-ESI-MS/MS approach on a Sciex 6500QTRAP mass spectrometer coupled with a Shimadzu Nexera X2 UHPLC system.

    Techniques: Mouse Assay, Liquid Chromatography, Mass Spectrometry, Whisker Assay

    The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.

    Journal: JCI Insight

    Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

    doi: 10.1172/jci.insight.98006

    Figure Lengend Snippet: The effect of differentiation and type 2 cytokines on keratinocyte lipids. ( A ) The effect of Ca 2+ -induced differentiation in vitro on relative proportion of selected ceramides in keratinocytes. Each ceramide molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total lipid phosphorus content, and data were expressed as relative percentage within each lipid subclass. Each individual data point was expressed relative to an average of nondifferentiated control. Note the decrease in relative content of short-chain ceramides and the increase in long-chain ceramides. STAT6 controls the effect of IL-4/IL-13 on NS ceramides with C18 sphingosine ( B ) and sphingomyelins ( C ) in differentiated keratinocytes. No differences in lysophosphatidylcholines (LPC) are observed in the keratinocyte model ( D ). Keratinocytes were differentiated in the absence or presence of IL-4/IL-13. Keratinocytes were treated with STAT6 siRNA during a 5-day differentiation period. Lipids were quantified by LC-ESI-MS/MS and normalized by total lipid phosphorus content ( n = 3). One from 2 typical experiments is shown; each treatment condition done in triplicates. Two-tailed Student’s t test was used for statistical analysis.

    Article Snippet: Ceramides, LPC, and SM were identified and quantified using the targeted LC-ESI-MS/MS approach on a Sciex 6500QTRAP mass spectrometer coupled with a Shimadzu Nexera X2 UHPLC system.

    Techniques: In Vitro, Liquid Chromatography, Mass Spectrometry, Two Tailed Test

    The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P

    Journal: JCI Insight

    Article Title: Lipid abnormalities in atopic skin are driven by type 2 cytokines

    doi: 10.1172/jci.insight.98006

    Figure Lengend Snippet: The effect of skin atopy on stratum corneum lipids. Changes in relative level of short- and long-chain molecular species in ceramides ( A ), sphingomyelins ( B ) and lysophosphatidylcholines ( C ) in stratum corneum of atopic dermatitis (AD) patients as compared with skin of normal control subjects. Each lipid molecular species was quantified by targeted liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and normalized by sample total protein content, and data were expressed as relative percentage within each lipid subclass. Data are presented as box-and-whisker plot, with whiskers showing minimum and maximum values. *, + P

    Article Snippet: Ceramides, LPC, and SM were identified and quantified using the targeted LC-ESI-MS/MS approach on a Sciex 6500QTRAP mass spectrometer coupled with a Shimadzu Nexera X2 UHPLC system.

    Techniques: Liquid Chromatography, Mass Spectrometry, Whisker Assay