mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
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    mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)


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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) <t>ELISA</t> of serum LPS-binding <t>protein</t> <t>(LBP).</t> (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse lipopolysaccharide binding protein elisa kit - by Bioz Stars, 2023-06
    94/100 stars

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    1) Product Images from "Endotoxin Producers Overgrowing in Human Gut Microbiota as the Causative Agents for Nonalcoholic Fatty Liver Disease"

    Article Title: Endotoxin Producers Overgrowing in Human Gut Microbiota as the Causative Agents for Nonalcoholic Fatty Liver Disease

    Journal: mBio

    doi: 10.1128/mBio.03263-19

    E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) ELISA of serum LPS-binding protein (LBP). (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Figure Legend Snippet: E. cloacae B29 induction of systemic and local inflammation in HFD-fed NAFLD GF mice is dependent upon endotoxin expression. GF mice were fed either normal chow diet (NCD) or high-fat diet (HFD) and inoculated with either Luria-Bertani broth (LB), wild-type E. cloacae B29, or the endotoxin-lacking waaG mutant strain (data were collected at the end of 15 weeks after inoculation). (A) ELISA of serum LPS-binding protein (LBP). (B) ELISA of serum amyloid A (SAA-3). (C) RT-qPCR analysis of expression of the Tlr4 gene in the liver. (D to F) RT-qPCR analysis of expression of the Tnfα , Il1β , Mcp1 , Ikkε , and Reg3γ genes in the liver (D), ileum (E), and epididymal fat pad (F). All mRNA quantification data were normalized against the housekeeping gene. Gene expression levels were expressed as values relative to those of the control group (NCD+LB). Tlr4 , toll-like receptor 4 gene; Tnfα , tumor necrosis factor-α gene; Il1β , interleukin-1β gene; Mcp1 , monocyte chemoattractant protein-1 gene; Ikkε , I kappa B kinase epsilon gene; Reg3γ , c-type lectin regenerating islet-derived protein 3-γ. Data shown are means ± SEM ( n = 5 to 10). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.

    Techniques Used: Expressing, Mutagenesis, Enzyme-linked Immunosorbent Assay, Binding Assay, Quantitative RT-PCR, Derivative Assay

    Absence of TLR4 in mice prevented NAFLD induced by the LPS-producing gut opportunistic pathobiont Enterobacter cloacae B29 (data were collected at the end of 15 weeks after inoculation). (A) Liver histology (hematoxylin and eosin stain) of TLR4 mutant (TLR4 −/− ) mice with or without B29 at the end of the trial. Scale bar, 100 μm. (B) NAFLD activity score. (C) Body weight. (D) Mass of epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal fat pads of TLR4 −/− mice with or without B29. (E) Plasma insulin concentration before (fasting) and 30 min after oral glucose load in TLR4 −/− mice with or without B29. (F) ELISA of serum leptin in TLR4 −/− mice with or without B29 (after adjustment for body weight). (G) ELISA of serum LBP in TLR4 −/− mice with or without B29. (H) ELISA of serum amyloid A (SAA-3) in TLR4 −/− mice with or without B29. Data shown are means ± SEM ( n = 5 to 9). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.
    Figure Legend Snippet: Absence of TLR4 in mice prevented NAFLD induced by the LPS-producing gut opportunistic pathobiont Enterobacter cloacae B29 (data were collected at the end of 15 weeks after inoculation). (A) Liver histology (hematoxylin and eosin stain) of TLR4 mutant (TLR4 −/− ) mice with or without B29 at the end of the trial. Scale bar, 100 μm. (B) NAFLD activity score. (C) Body weight. (D) Mass of epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal fat pads of TLR4 −/− mice with or without B29. (E) Plasma insulin concentration before (fasting) and 30 min after oral glucose load in TLR4 −/− mice with or without B29. (F) ELISA of serum leptin in TLR4 −/− mice with or without B29 (after adjustment for body weight). (G) ELISA of serum LBP in TLR4 −/− mice with or without B29. (H) ELISA of serum amyloid A (SAA-3) in TLR4 −/− mice with or without B29. Data shown are means ± SEM ( n = 5 to 9). * , P < 0.05; * * , P < 0.01; ** * , P < 0.001.

    Techniques Used: H&E Stain, Mutagenesis, Activity Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

    lbp soluble elisa kit  (Hycult Biotech)


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    Hycult Biotech lbp soluble elisa kit
    Lbp Soluble Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbp soluble elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    lbp soluble elisa kit - by Bioz Stars, 2023-06
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    hbt mouse lbp elisa test kit  (Hycult Biotech)


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    Hycult Biotech hbt mouse lbp elisa test kit
    Hbt Mouse Lbp Elisa Test Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbt mouse lbp elisa test kit/product/Hycult Biotech
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    hbt mouse lbp elisa test kit - by Bioz Stars, 2023-06
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    lbp elisa kit  (Hycult Biotech)


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    Hycult Biotech lbp elisa kit
    Lbp Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbp elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    lbp elisa kit - by Bioz Stars, 2023-06
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    lbp elisa kit  (Hycult Biotech)


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    Hycult Biotech lbp elisa kit
    Lbp Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbp elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    lbp elisa kit - by Bioz Stars, 2023-06
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    plasma lbp  (Hycult Biotech)


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    Hycult Biotech plasma lbp
    In vitro validation of <t>Lbp</t> siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on <t>plasma</t> <t>LBP</t> at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.
    Plasma Lbp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma lbp/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    plasma lbp - by Bioz Stars, 2023-06
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    1) Product Images from "Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation"

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2022.08.003

    In vitro validation of Lbp siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on plasma LBP at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.
    Figure Legend Snippet: In vitro validation of Lbp siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on plasma LBP at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

    Techniques Used: In Vitro, In Vivo, Modification, Sequencing, Injection, Expressing, Concentration Assay

    Association of liver LBP with expression of de novo lipogenesis genes in morbidly obese human subjects (A and B) Plasma LBP concentration (A) and liver, SAT, and VAT LBP mRNA levels (B) according to NAFLD. (C) Bivariate correlations between liver LBP mRNA levels and plasma LBP concentration in morbidly obese participants. (D–F) Bivariate correlations between liver LBP and SREBF1 (D), FASN (E), and ACACA (F) mRNA levels. (G) Bivariate correlations between plasma LBP concentration and liver FASN mRNA levels.
    Figure Legend Snippet: Association of liver LBP with expression of de novo lipogenesis genes in morbidly obese human subjects (A and B) Plasma LBP concentration (A) and liver, SAT, and VAT LBP mRNA levels (B) according to NAFLD. (C) Bivariate correlations between liver LBP mRNA levels and plasma LBP concentration in morbidly obese participants. (D–F) Bivariate correlations between liver LBP and SREBF1 (D), FASN (E), and ACACA (F) mRNA levels. (G) Bivariate correlations between plasma LBP concentration and liver FASN mRNA levels.

    Techniques Used: Expressing, Concentration Assay

    lbp elisa kit  (Hycult Biotech)


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    Hycult Biotech lbp elisa kit
    Lbp Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbp elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lbp elisa kit - by Bioz Stars, 2023-06
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    lbp mouse elisa kit  (Hycult Biotech)


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    Hycult Biotech lbp mouse elisa kit
    Lbp Mouse Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lbp mouse elisa kit/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
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    lbp mouse elisa kit - by Bioz Stars, 2023-06
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    plasma lbp  (Hycult Biotech)


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    Hycult Biotech plasma lbp
    Bivariate correlations between <t> plasma LBP </t> levels and liver, iWAT or pgWAT Lbp mRNA levels
    Plasma Lbp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma lbp/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasma lbp - by Bioz Stars, 2023-06
    94/100 stars

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    1) Product Images from "Specific adipose tissue Lbp gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression"

    Article Title: Specific adipose tissue Lbp gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2022.01.002

    Bivariate correlations between plasma LBP levels and liver, iWAT or pgWAT Lbp mRNA levels
    Figure Legend Snippet: Bivariate correlations between plasma LBP levels and liver, iWAT or pgWAT Lbp mRNA levels

    Techniques Used:

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    • mouse lipopolysaccharide binding protein elisa kit  (Hycult Biotech)
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    Hycult Biotech mouse lipopolysaccharide binding protein elisa kit
    Mouse Lipopolysaccharide Binding Protein Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse lipopolysaccharide binding protein elisa kit/product/Hycult Biotech
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    lbp soluble elisa kit  (Hycult Biotech)
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    hbt mouse lbp elisa test kit  (Hycult Biotech)
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    lbp elisa kit  (Hycult Biotech)
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    Lbp Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    plasma lbp  (Hycult Biotech)
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    In vitro validation of <t>Lbp</t> siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on <t>plasma</t> <t>LBP</t> at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.
    Plasma Lbp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lbp mouse elisa kit  (Hycult Biotech)
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    In vitro validation of <t>Lbp</t> siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on <t>plasma</t> <t>LBP</t> at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.
    Lbp Mouse Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    In vitro validation of Lbp siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on plasma LBP at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    doi: 10.1016/j.omtn.2022.08.003

    Figure Lengend Snippet: In vitro validation of Lbp siRNAs and in vivo effect of Lbp UNA-siRNA delivered through lipid nanoparticles (A) Effect of several doses of chemically unmodified and modified (UNA) siRNA (si69107, si69108, si69109) against Lbp mRNA sequence in Hepa1-6 cell line. (B) Effect of chemically unmodified (1 and 3 mg/kg) and chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on plasma LBP at days 3, 6, 10, and 12 after injection. (C) Effect of chemically modified (UNA, 1 and 3 mg/kg) LNP- Lbp siRNA on liver Lbp gene expression 3 days after second injection. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with 0 pmol/L or vehicle. (D) Effects of 6-month LNP- Lbp UNA-siRNA administration in HFHS-fed mice on liver, inguinal WAT (iWAT), and perigonadal WAT (pgWAT) Lbp mRNA levels and plasma Lbp concentration in male and female mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

    Article Snippet: Plasma LBP (HK205-02, LBP mouse ELISA kit, Hycult Biotech, Plymouth Meeting, PA, USA), insulin (90080, Crystal Chem, Zaandam, the Netherlands), glucose (Accutrend; Roche Diagnostics, Mannheim, Germany), and ALT activity (Alfa Wasserman/Vet Axcel Reagent SA1046) were measured using commercial kits according to manufacturer’s instructions.

    Techniques: In Vitro, In Vivo, Modification, Sequencing, Injection, Expressing, Concentration Assay

    Association of liver LBP with expression of de novo lipogenesis genes in morbidly obese human subjects (A and B) Plasma LBP concentration (A) and liver, SAT, and VAT LBP mRNA levels (B) according to NAFLD. (C) Bivariate correlations between liver LBP mRNA levels and plasma LBP concentration in morbidly obese participants. (D–F) Bivariate correlations between liver LBP and SREBF1 (D), FASN (E), and ACACA (F) mRNA levels. (G) Bivariate correlations between plasma LBP concentration and liver FASN mRNA levels.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    doi: 10.1016/j.omtn.2022.08.003

    Figure Lengend Snippet: Association of liver LBP with expression of de novo lipogenesis genes in morbidly obese human subjects (A and B) Plasma LBP concentration (A) and liver, SAT, and VAT LBP mRNA levels (B) according to NAFLD. (C) Bivariate correlations between liver LBP mRNA levels and plasma LBP concentration in morbidly obese participants. (D–F) Bivariate correlations between liver LBP and SREBF1 (D), FASN (E), and ACACA (F) mRNA levels. (G) Bivariate correlations between plasma LBP concentration and liver FASN mRNA levels.

    Article Snippet: Plasma LBP (HK205-02, LBP mouse ELISA kit, Hycult Biotech, Plymouth Meeting, PA, USA), insulin (90080, Crystal Chem, Zaandam, the Netherlands), glucose (Accutrend; Roche Diagnostics, Mannheim, Germany), and ALT activity (Alfa Wasserman/Vet Axcel Reagent SA1046) were measured using commercial kits according to manufacturer’s instructions.

    Techniques: Expressing, Concentration Assay