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Thermo Fisher lb broth
Lb Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lb broth/product/Thermo Fisher
Average 99 stars, based on 39 article reviews
Price from $9.99 to $1999.99
lb broth - by Bioz Stars, 2020-04
99/100 stars

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Related Articles

Clone Assay:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol. .. Positive clones were confirmed by PCR amplification as for the original reactions (NR using second-round conditions), omitting BSA and using 1.5 μl of the transformed cell culture as the template for the reaction.

Centrifugation:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Expression was induced through addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Thermo Fischer Scientific) and incubated for 3 h. Cells were harvested by centrifugation at 6,000 × g (Beckman J-10) for 20 min. Purification was carried out by lysing the cells with 2 mg/ml lysozyme (Sigma-Aldrich) and pelleting cell debris through centrifugation.

Amplification:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: Paragraph title: 18S and NR gene amplification, cloning, and Sanger sequencing. ... Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

TA Cloning:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: For each sample, PCR products were pooled, purified using the QIAquick PCR purification kit (Qiagen), and then cloned using the Topo TA cloning kit (Invitrogen, Burlington, Canada), following the manufacturer’s instructions. .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

Construct:

Article Title: The sRNA DicF integrates oxygen sensing to enhance enterohemorrhagic Escherichia coli virulence via distinctive RNA control mechanisms
Article Snippet: Unless indicated otherwise, bacteria were grown statically overnight in LB broth, diluted 1:100 in low-glucose DMEM (Invitrogen), and grown statically for 6 h at 37 °C, 5% CO2 (microaerobic conditions). .. Deletion strains were constructed using lambda red mutagenesis ( ).

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: The mutation C291S was added by site directed mutagenesis and this construct is referred to as tau187 throughout the manuscript. .. Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved.

Incubation:

Article Title: Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky
Article Snippet: Cultures were incubated in 96-well plates at 37°C with shaking in a SpectraMax Plus384 spectrophotometer. .. Growth media tested included LB broth, LB broth plus 2,2′-dipyridyl, 50% filtered human serum in LB broth (Life Technologies, Carlsbad, CA), and 100% filtered chicken serum (Sigma-Aldrich Corporation, St. Louis, MO).

Article Title: Effects of Exposure Time and Biological State on Acquisition and Accumulation of Erwinia amylovora by Drosophila melanogaster
Article Snippet: Cultures were transferred weekly; a sterile inoculation loop was dipped into E. amylovora colonies on CG medium and grown overnight in LB broth (catalog no. 12795-027; Invitrogen) at 28°C in a shake incubator. .. One microliter of LB broth-grown E. amylovora was plated on CG plates and incubated upside down at 28°C with high humidity for 48 h and then stored at 4°C until used.

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Expression was induced through addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Thermo Fischer Scientific) and incubated for 3 h. Cells were harvested by centrifugation at 6,000 × g (Beckman J-10) for 20 min. Purification was carried out by lysing the cells with 2 mg/ml lysozyme (Sigma-Aldrich) and pelleting cell debris through centrifugation.

Expressing:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Paragraph title: Protein Expression and Purification ... Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved.

Transformation Assay:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol. .. Positive clones were confirmed by PCR amplification as for the original reactions (NR using second-round conditions), omitting BSA and using 1.5 μl of the transformed cell culture as the template for the reaction.

Transfection:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: .. Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Expression was induced through addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Thermo Fischer Scientific) and incubated for 3 h. Cells were harvested by centrifugation at 6,000 × g (Beckman J-10) for 20 min. Purification was carried out by lysing the cells with 2 mg/ml lysozyme (Sigma-Aldrich) and pelleting cell debris through centrifugation.

Cell Culture:

Article Title: Comprehensive quantitation of RNA-protein interaction dynamics by orthogonal organic phase separation (OOPS)
Article Snippet: .. E. coli K-12 DH5a strain (Thermo Fisher Scientific), was cultured in LB Broth (Thermo Fisher Scientific) at 37 ºC. ..

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol. .. Positive clones were confirmed by PCR amplification as for the original reactions (NR using second-round conditions), omitting BSA and using 1.5 μl of the transformed cell culture as the template for the reaction.

other:

Article Title: Detection of Salmonella-specific antibody in swine oral fluids
Article Snippet: Heat-inactivated Salmonella SB377 statically-grown (37 °C overnight) in LB broth (Invitrogen, Carlsbad, CA) was pelleted, washed, and resuspended in phosphate buffered saline (PBS).

Sequencing:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: Paragraph title: 18S and NR gene amplification, cloning, and Sanger sequencing. ... Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

Binding Assay:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Tau was extracted from the supernatant by binding to nickel affinity resin and subsequently removed by washing with increasing concentration of imidazole buffers.

Mutagenesis:

Article Title: The sRNA DicF integrates oxygen sensing to enhance enterohemorrhagic Escherichia coli virulence via distinctive RNA control mechanisms
Article Snippet: Unless indicated otherwise, bacteria were grown statically overnight in LB broth, diluted 1:100 in low-glucose DMEM (Invitrogen), and grown statically for 6 h at 37 °C, 5% CO2 (microaerobic conditions). .. Deletion strains were constructed using lambda red mutagenesis ( ).

Article Title: Genome-Wide Identification of Fitness Factors in Seawater for Edwardsiella piscicida
Article Snippet: E. piscicida EIB202 was used as the parental strain for the construction of indicated mutant strains ( ). .. E. piscicida strains were grown in LB broth or LB agar (Oxoid, England) and naturally filtered seawater obtained from an aquaculture farm in Yantai, Shandong Province, at 16 or 28°C.

Article Title: Genome-Wide Identification of Fitness Factors in Seawater for Edwardsiella piscicida
Article Snippet: E. piscicida EIB202 was used as the parental strain for the construction of indicated mutant strains ( ). .. E. piscicida strains were grown in LB broth or LB agar (Oxoid, England) and naturally filtered seawater obtained from an aquaculture farm in Yantai, Shandong Province, at 16 or 28°C.

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: The mutation C291S was added by site directed mutagenesis and this construct is referred to as tau187 throughout the manuscript. .. Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved.

Purification:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: For each sample, PCR products were pooled, purified using the QIAquick PCR purification kit (Qiagen), and then cloned using the Topo TA cloning kit (Invitrogen, Burlington, Canada), following the manufacturer’s instructions. .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Paragraph title: Protein Expression and Purification ... Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved.

Polymerase Chain Reaction:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: For each sample, PCR products were pooled, purified using the QIAquick PCR purification kit (Qiagen), and then cloned using the Topo TA cloning kit (Invitrogen, Burlington, Canada), following the manufacturer’s instructions. .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

CRISPR:

Article Title: Effects of Exposure Time and Biological State on Acquisition and Accumulation of Erwinia amylovora by Drosophila melanogaster
Article Snippet: Overall, E. amylovora strain diversity is low and requires CRISPR spacer array analysis to properly characterize, so use of a single strain in this experiment was appropriate ( ). .. Cultures were transferred weekly; a sterile inoculation loop was dipped into E. amylovora colonies on CG medium and grown overnight in LB broth (catalog no. 12795-027; Invitrogen) at 28°C in a shake incubator.

Nested PCR:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: A second-round, nested PCR was performed for NR as described above except that annealing was performed at 55°C, extension was for only 30 s, and 2 μl of the first-round product was used as the template. .. Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol.

Plasmid Preparation:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol. .. For each NR clone library, 16 to 25 positive clones, as confirmed by agarose gel electrophoresis, were randomly selected to be sequenced using the vector M13 primer sites.

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: .. Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Expression was induced through addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Thermo Fischer Scientific) and incubated for 3 h. Cells were harvested by centrifugation at 6,000 × g (Beckman J-10) for 20 min. Purification was carried out by lysing the cells with 2 mg/ml lysozyme (Sigma-Aldrich) and pelleting cell debris through centrifugation.

Affinity Chromatography:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Protein Expression and Purification A segment of tau, , consisting of residues 255-441 was expressed in E. coli BL21 (DE3) and purified via nickel affinity chromatography as previously described ( ). .. Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved.

Agarose Gel Electrophoresis:

Article Title: Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
Article Snippet: Transformed clones were picked into 96-well plates and cultured overnight at 37°C in LB broth with ampicillin (Fermentas) and 7% glycerol. .. For each NR clone library, 16 to 25 positive clones, as confirmed by agarose gel electrophoresis, were randomly selected to be sequenced using the vector M13 primer sites.

In Vitro:

Article Title: Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky
Article Snippet: Kentucky isolates for their relative in vitro growth capabilities, the growth rates of selected Salmonella isolates were compared in LB broth. .. Growth media tested included LB broth, LB broth plus 2,2′-dipyridyl, 50% filtered human serum in LB broth (Life Technologies, Carlsbad, CA), and 100% filtered chicken serum (Sigma-Aldrich Corporation, St. Louis, MO).

Spectrophotometry:

Article Title: Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky
Article Snippet: Cultures were incubated in 96-well plates at 37°C with shaking in a SpectraMax Plus384 spectrophotometer. .. Growth media tested included LB broth, LB broth plus 2,2′-dipyridyl, 50% filtered human serum in LB broth (Life Technologies, Carlsbad, CA), and 100% filtered chicken serum (Sigma-Aldrich Corporation, St. Louis, MO).

Concentration Assay:

Article Title: Tau-Cofactor Complexes as Building Blocks of Tau Fibrils
Article Snippet: Briefly, BL21 (DE3) cells transfected with a pET28a vector including the tau187 genes were grown in 1 L cultures of LB broth (Thermo Fisher Scientific) until an optical density of 0.6 A600 was achieved. .. Tau was extracted from the supernatant by binding to nickel affinity resin and subsequently removed by washing with increasing concentration of imidazole buffers.

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    Thermo Fisher luria bertani lb broth
    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, <t>Luria–Bertani.</t>
    Luria Bertani Lb Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb broth/product/Thermo Fisher
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb broth - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher luria broth lb microbial growth medium
    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, <t>Luria–Bertani.</t>
    Luria Broth Lb Microbial Growth Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria broth lb microbial growth medium/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luria broth lb microbial growth medium - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    Image Search Results


    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Journal: International Journal of Nanomedicine

    Article Title: Antibacterial and biodegradable tissue nano-adhesives for rapid wound closure

    doi: 10.2147/IJN.S177109

    Figure Lengend Snippet: Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Article Snippet: Luria–Bertani (LB) broth was purchased from Thermo Fisher Scientific.

    Techniques:

    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Journal: International Journal of Nanomedicine

    Article Title: Antibacterial and biodegradable tissue nano-adhesives for rapid wound closure

    doi: 10.2147/IJN.S177109

    Figure Lengend Snippet: Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Article Snippet: Luria–Bertani (LB) broth was purchased from Thermo Fisher Scientific.

    Techniques: