Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Underexpression of Lats2 increases the retention of BMSCs in ALI lung tissue. (a) Immunofluorescence staining images of lungs in the MSC-shcontrol and MSC-shLats2 groups are presented from six mouse lungs obtained 3, 7, and 14 days after LPS challenge. The nuclei were stained with DAPI (blue), and the engrafted BMSCs in the lung tissue are shown as GFP-positive (green; magnification, ×400; scale bar = 20 μ m; white arrows, GFP-positive cells). The count of GFP-positive BMSCs in randomly selected high-power fields is presented as the mean ± standard deviation ( n = 6). (b) Ex vivo NIR imaging of lungs in the MSC-shcontrol and MSC-shLats2 groups are shown from six mouse lungs obtained 3, 7, and 14 days after LPS challenge. ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Immunofluorescence, Staining, Standard Deviation, Ex Vivo, Imaging

Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Underexpression of Lats2 promotes the differentiation of BMSCs into ATII cells. (a) Immunofluorescence staining images of lungs in the MSC-shcontrol and MSC-shLats2 groups are presented from six mouse lungs obtained 14 days after LPS challenge. Engrafted BMSCs, ATII cells, or BMSCs that differentiated into ATII cells in the lung are shown as GFP-positive (green), SPC-positive (red), or double-positive (yellow) under fluorescence microscopy, respectively. The nuclei were stained with DAPI (blue; magnification, ×400; scale bar = 20 μ m). The ratio of the count of SPC-positive to the count of GFP-positive BMSCs in randomly selected high-power fields is presented as the mean ± standard deviation ( n = 6). (b) The protein expression of SPC in the lung tissue was measured using Western blot analysis 14 days after LPS challenge. β -Actin was used as the internal reference, and the results are presented as the mean ± standard deviation ( n = 6). ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Immunofluorescence, Staining, Fluorescence, Microscopy, Standard Deviation, Expressing, Western Blot

Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Lats2-underexpressing BMSCs improve lung edema and permeability of lung epithelium. (a) Protein expression of occludin in the lung tissue was measured using Western blot analysis at 14 days after LPS exposure. β -Actin was used as the internal reference. (b) Lung edema was measured by the LWW/BW at 3 and 14 days after LPS challenge. (c) TP in the BALF was measured using ELISA at 3 and 14 days after LPS challenge. (d) ALB in the BALF was measured using ELISA at 3 and 14 days after LPS challenge. The results are presented as the mean ± standard deviation of values obtained from six mice per group at each time point. ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Permeability, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Lats2-underexpressing BMSCs improve acute lung inflammation. The mRNA expressions of proinflammatory cytokines (a) IL-1 β and (b) IL-6 and anti-inflammatory cytokines (c) IL-4 and (d) IL-10 in the lung homogenate were measured using RT-qPCR at 24 h after LPS challenge. GAPDH was used as the internal reference gene ( n = 3). The concentrations of (a) IL-1 β , (b) IL-6, (c) IL-4, and (d) IL-10 in the BALF were measured using ELISA at 3 days after LPS challenge ( n = 6). Data are presented as the mean ± standard deviation. ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Lats2-underexpressing BMSCs alleviate pathological injuries in ALI lung tissue. (a) Histopathological analysis of lung tissues was performed at 3 and 14 days after LPS challenge. (HE staining; magnification, ×200; scale bar = 100 μ m). (b) Lung injury score at 3 days after LPS challenge. (c) Lung injury score at 14 days after LPS challenge. Lung injury score is expressed as arbitrary unit. Data are presented as the mean ± standard deviation ( n = 3). ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Staining, Standard Deviation

Journal: Mediators of Inflammation

Article Title: Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

doi: 10.1155/2019/4851431

Figure Lengend Snippet: Lats2-underexpressing BMSCs inhibit early pulmonary fibrosis. (a) Picrosirius red staining of collagen fibers (red staining) in the lung tissues at 14 days after LPS challenge. (Picrosirius red staining; magnification, ×200; scale bar = 50 μ m). (b) Quantification of collagen area in Picrosirius red stained-lung sections at 14 days after LPS challenge. (c) Immunohistochemistry for α -SMA (dark brown staining) in the lung tissues at 14 days after LPS challenge (magnification, ×400; scale bar = 200 μ m). (d) Quantification of mean optical density (MOD) for α -SMA in the lung tissues at 14 days after LPS challenge. (e) Immunohistochemistry for TGF- β 1 (dark brown staining) in the lung tissues at 14 days after LPS challenge (magnification, ×400; scale bar = 200 μ m). (f) Quantification of MOD for TGF- β 1 in the lung tissues at 14 days after LPS challenge. (g) Hydroxyproline content of lung homogenates at 14 days after LPS challenge. (h) The mRNA expression of Collagen Type I Alpha 1 (Col1 α 1) in the lung tissue at 7 days after LPS challenge. GAPDH was used as the internal reference gene. (i) The mRNA expression of Collagen Type III Alpha 1 (Col3 α 1) in the lung tissue at 7 days after LPS challenge. GAPDH was used as the internal reference gene. Data are presented as the mean ± standard deviation ( n = 3). ∗ P < 0.05.

Article Snippet: After successful and stable transfection, BMSCs carrying enhanced green fluorescence protein (eGFP) and BMSCs carrying the Lats2-shRNA gene or the empty vector were cultured and passaged in a 1 : 1 mix of Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) culture medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% streptomycin and penicillin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Staining, Immunohistochemistry, Expressing, Standard Deviation

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) Pan-cancer analysis expression of LATS2 based on the TIMER1.0 database. (B) Meta-analysis expression of LATS2 in ESCC in the ONCOMINE database.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: Relationship between clinical factors and LATS2 expression in ESCC.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: The differential expression of LATS2 in TCGA (A) , GSE23400 (B) , and GSE161533 (C) datasets.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) Top graph represents the scatter plot of LATS2 expression from low to high; the middle graph refers to the scatter plot distribution of survival time and survival status corresponding to LATS2 gene expression in different samples; and the bottom graph represents the expression heat map of LATS2. (B) ROC curve and AUC assessed the performance of LATS2. (C) Kaplan–Meier survival analysis revealed that patients with increased LATS2 expression had longer OS in Asian, grade 2, male, and stage 3.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) Gene–gene interaction network for LATS2 and the altered neighboring genes constructed on GeneMANIA. (B) Protein–protein interaction identification on the STRING database.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Construct

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) Correlation analysis between LATS2 and other genes in ESCC. (B) GO enrichment analysis about the biological process. (C) GO enrichment analysis about molecular function. (D) GO enrichment analysis about cellular components. (E) KEGG enrichment analysis.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques:

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) LATS2 expression correlated with numbers of immune cells. (B) Correlation between LATS2 expression and immune checkpoints. (C) IHC staining of LATS2, CTLA4, and PD‐L1 in ESCC samples.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Genetics

Article Title: Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

doi: 10.3389/fgene.2022.952528

Figure Lengend Snippet: (A) Forest plot shows the prognostic value of LATS2 expression according to different immune cell subgroups in ESCC patients. (B) Kaplan–Meier plot was used to estimate the correlation between LATS2 expression and OS in different immune cell subgroups of ESCC patients.

Article Snippet: We utilized GraphPad Prism 8.0 to map LATS2 gene expression differences in ESCC.

Techniques: Expressing