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Promega lambda phage dna
Lambda Phage Dna, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda phage dna/product/Promega
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
lambda phage dna - by Bioz Stars, 2019-10
92/100 stars

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Methylation Sequencing:

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Paragraph title: Sample preparation and whole-genome bisulfite sequencing ... Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency.

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: Colony 1 generated sufficient fertilized (1280) and thelytokous (870) embryos for whole genome bisulfite sequencing. .. We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ].

Clone Assay:

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C. .. Then, 100 ng of the DNA was bisulfite converted and three lambda loci were amplified by polymerase chain reaction (PCR) (95 °C for 30 s followed by 15 cycles of 95 °C for 30 s, 61 °C for 30 s, and 72 °C for 30 s).

Amplification:

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C. .. Near complete methylation was confirmed by the resistance to methylation-sensitive restriction enzyme Hpa II (New England BioLabs).

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. PCR amplification was performed in a reaction mixture (10 µL) containing 10 ng of total genomic DNA, 0.4 µM of each primer and 5 µL of GoTaq Green Master Mix (Promega) and with the use of a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA).

Filtration:

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: The amount of 14 CO2 taken up by the So floc-associated community was based on the amount of radioactivity retained by the membrane filter after sample filtration (static incubations) or from the amount of radioactivity associated with the glass fiber matrix recovered from the CFRs after the radioactivity associated with killed controls was subtracted. .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard.

Positive Control:

Article Title: Increased Levels of Multiresistant Bacteria and Resistance Genes after Wastewater Treatment and Their Dissemination into Lake Geneva, Switzerland
Article Snippet: The final reaction volume of 20 μl contained 2 μl of 10× multicore buffer, 0.2 μl of enzyme (2 u), 7.8 μl water and 10 μl of PCR product. .. Lambda phage DNA (Promega) served as positive control. .. RFLP digests were visualized by gel electrophoresis on 3% NuSieve Agarose gels (Cambrex Bio Science Rockland, Inc., ME, USA) and stained with GelRed™ (Biotium, Inc., Hayward, CA, USA).

Lambda DNA Preparation:

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: Paragraph title: Methylation of lambda DNA ... To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C.

SYBR Green Assay:

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. Strain-specific forward primers for isolate 18U65 and 18D70 were designed from the nearly full-length 16S rRNA gene.

Incubation:

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: For experiments using CFRs, the concentration of NaH[14 C]O3 in the system at any point in time during the 2-h incubation was calculated from the concentration of NaH[14 C]O3 in the feed reservoir and the dilution factor due to mixing with water pumped directly from the spring's source before entering the reactors. .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard.

Touchdown PCR:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. The amplification protocol comprised an initial denaturation for 2 min at 95°C; 30 cycles of denaturation for 30 s at 95°C, annealing for 30 s at 47°C or 55°C, and extension for 30 s at 72°C; and a final extension for 5 min at 72°C.

Modification:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. The amplification protocol comprised an initial denaturation for 2 min at 95°C; 30 cycles of denaturation for 30 s at 95°C, annealing for 30 s at 47°C or 55°C, and extension for 30 s at 72°C; and a final extension for 5 min at 72°C.

Electron Microscopy:

Article Title: Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels
Article Snippet: Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1. .. The device was mounted on an inverted fluorescence microscope (Nikon) and imaged through a ×100/1.4 NA oil immersion objective (Nikon) as DNA molecules were being electrokinetically driven through the microfluidic and nanofluidic channels.

Serial Dilution:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: Total genomic DNA was extracted from young leaves or seed flour of individual samples with the use of an Automatic DNA Isolation System PI-50a (Kurabo, Osaka, Japan) according to Plant DNA Extraction Protocol (version 2). .. The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. PCR amplification was performed in a reaction mixture (10 µL) containing 10 ng of total genomic DNA, 0.4 µM of each primer and 5 µL of GoTaq Green Master Mix (Promega) and with the use of a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA).

Generated:

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: Colony 1 generated sufficient fertilized (1280) and thelytokous (870) embryos for whole genome bisulfite sequencing. .. We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ].

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

CpG Methylation Assay:

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C. .. The PCR products were cloned into pMD20 (TaKaRa) and sequenced.

Polymerase Chain Reaction:

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ]. .. Bisulfite conversion, library construction and sequencing were performed at the Beijing Genomics Institute as described in Drewell et al. [ ].

Article Title: Increased Levels of Multiresistant Bacteria and Resistance Genes after Wastewater Treatment and Their Dissemination into Lake Geneva, Switzerland
Article Snippet: The final reaction volume of 20 μl contained 2 μl of 10× multicore buffer, 0.2 μl of enzyme (2 u), 7.8 μl water and 10 μl of PCR product. .. Lambda phage DNA (Promega) served as positive control.

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C. .. To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C.

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. The EST-derived SSR markers were amplified with the modified ‘Touchdown PCR’ protocol.

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Binding Assay:

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. Strain-specific forward primers for isolate 18U65 and 18D70 were designed from the nearly full-length 16S rRNA gene.

Molecular Weight:

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency. .. Carver Biotechnology Center at the University of Illinois for quality control, library construction, and sequencing.

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard. .. The conversion factor (7.0 fg of DNA cell−1 ) was based on the size of the genome (3.2 Mb) of Hydrogenobaculum sp. strain 3684, a chemolithoautotroph belonging to the Aquificaceae within the Aquificae previously isolated from Dragon Spring ( ).

DNA Extraction:

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Individual brains were placed in microfuge tubes and stored at − 80 °C until DNA extraction. .. Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency.

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: Paragraph title: Sample collection, DNA extraction and sequencing ... We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ].

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: Paragraph title: DNA extraction and marker analysis ... The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water.

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Radioactivity:

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: The amount of 14 CO2 taken up by the So floc-associated community was based on the amount of radioactivity retained by the membrane filter after sample filtration (static incubations) or from the amount of radioactivity associated with the glass fiber matrix recovered from the CFRs after the radioactivity associated with killed controls was subtracted. .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard.

Fluorescence:

Article Title: Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels
Article Snippet: Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1. .. Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1.

Methylation:

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency. .. The integrity of the samples was checked using the AATI Fragment Analyzer, which confirmed that the DNA was of high molecular weight.

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: IMR-90, a human fibroblast cell line, was cultured as previously described [ ]. .. To generate methylated control DNA, 1 μg of lambda phage DNA (Promega) was methylated with CpG methyltransferase Sss I (New England BioLabs) for 3 h at 37 °C. .. Near complete methylation was confirmed by the resistance to methylation-sensitive restriction enzyme Hpa II (New England BioLabs).

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: Summary of PBAT-seq with human and mouse genomic DNA using different HCS and RTA versions. .. Summary of PBAT-seq with in vitro methylated lambda phage DNA. .. Summary of the effect of cluster density on PBAT-seq using different HCS versions.

Isolation:

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard. .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard.

Microscopy:

Article Title: Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels
Article Snippet: Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1. .. Solutions containing 0.5 ng μL−1 of DNA (16 pM for λ-phage and 4.6 pM for T4-phage) also contained 4% (by volume) β-mercaptoethanol (Fisher Scientific) to limit photo-induced damage and 2% (by mass) polyvinylpyrrolidone (PVP, 10 kDa, Sigma-Aldrich) to reduce electro-osmotic flow within the channels.

Purification:

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: Embryos were lysed in a tissue lyser and treated with 2 mg/ml RNAse at 37 °C for 30 min, Proteinase K at 56 °C for two hours, and DNA purified using 20G genomic tips (Qiagen). .. We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ].

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: The rate of CO2 uptake by the So floc-associated microbial community was normalized to DNA as previously described ( ). .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard. .. This approach yielded 2.44 ± 0.53 μg of DNA g−1 (dry weight) of So floc−1 .

Sequencing:

Article Title: Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos
Article Snippet: Five hundred to 1,000 oocytes or 30–40 blastocysts were spiked with 0.1 ng of lambda phage DNA (Promega) and subjected to bisulfite conversion. .. Five hundred to 1,000 oocytes or 30–40 blastocysts were spiked with 0.1 ng of lambda phage DNA (Promega) and subjected to bisulfite conversion.

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency. .. Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency.

Article Title: Parent-of-origin effects on genome-wide DNA methylation in the Cape honey bee (Apis mellifera capensis) may be confounded by allele-specific methylation
Article Snippet: Paragraph title: Sample collection, DNA extraction and sequencing ... We added lambda bacteriophage DNA (0.1 % (w/w), Promega) to each sample as a spike-in control to evaluate the efficacy of the bisulfite conversion [ ].

Article Title: Increased Levels of Multiresistant Bacteria and Resistance Genes after Wastewater Treatment and Their Dissemination into Lake Geneva, Switzerland
Article Snippet: Paragraph title: Identification of multiple resistant bacterial isolates from wastewater by 16S rDNA gene fragment sequencing ... Lambda phage DNA (Promega) served as positive control.

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Polyacrylamide Gel Electrophoresis:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. The EST-derived SSR markers were amplified with the modified ‘Touchdown PCR’ protocol.

Real-time Polymerase Chain Reaction:

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: Paragraph title: qPCR analysis. ... DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Negative Control:

Article Title: Increased Levels of Multiresistant Bacteria and Resistance Genes after Wastewater Treatment and Their Dissemination into Lake Geneva, Switzerland
Article Snippet: 10 ng of DNA from E. coli served as a positive control and 1 μl of nuclease-free water (Qiagen, Germany) served as negative control in all PCRs carried out in this study. .. Lambda phage DNA (Promega) served as positive control.

Denaturing Gradient Gel Electrophoresis:

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Sample Prep:

Article Title: Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain
Article Snippet: Paragraph title: Sample preparation and whole-genome bisulfite sequencing ... Unmethylated lambda phage DNA (Promega) was spiked in as a control for assessment of bisulfite conversion efficiency.

In Vitro:

Article Title: Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing
Article Snippet: Summary of PBAT-seq with human and mouse genomic DNA using different HCS and RTA versions. .. Summary of PBAT-seq with in vitro methylated lambda phage DNA. .. Summary of the effect of cluster density on PBAT-seq using different HCS versions.

Flocculation:

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: The rate of CO2 uptake by the So floc-associated microbial community was normalized to DNA as previously described ( ). .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard. .. This approach yielded 2.44 ± 0.53 μg of DNA g−1 (dry weight) of So floc−1 .

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard.

Spectrophotometry:

Article Title: Increased Levels of Multiresistant Bacteria and Resistance Genes after Wastewater Treatment and Their Dissemination into Lake Geneva, Switzerland
Article Snippet: Lambda phage DNA (Promega) served as positive control. .. Isolates were grouped into ribo-types according to identical restriction patterns and one representative of each group was selected for sequencing.

Concentration Assay:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: Total genomic DNA was extracted from young leaves or seed flour of individual samples with the use of an Automatic DNA Isolation System PI-50a (Kurabo, Osaka, Japan) according to Plant DNA Extraction Protocol (version 2). .. The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water. .. PCR amplification was performed in a reaction mixture (10 µL) containing 10 ng of total genomic DNA, 0.4 µM of each primer and 5 µL of GoTaq Green Master Mix (Promega) and with the use of a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA).

Article Title: CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
Article Snippet: For experiments using CFRs, the concentration of NaH[14 C]O3 in the system at any point in time during the 2-h incubation was calculated from the concentration of NaH[14 C]O3 in the feed reservoir and the dilution factor due to mixing with water pumped directly from the spring's source before entering the reactors. .. Briefly, DNA was extracted from 1.0 mg of So floc, reacted with the DNA-binding fluorophore SYBR Gold I (Invitrogen, Carlsbad, CA), and quantified with a NanoDrop 3300 fluorimeter (Thermo Scientific, Wilmington, DE) using purified lambda phage DNA (Promega, Madison, WI) as a standard.

Article Title: Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park
Article Snippet: At the laboratory, samples of S0 floc were treated to remove residual S0 and subjected to total DNA extraction as described above for DGGE analysis. .. DNA was quantified using the nucleic acid binding fluorochrome SYBR Green I (Invitrogen) added to achieve a final concentration of 1/1,000 (vol/vol), an ND-3300 Fluorospectrometer (NanoDrop Technologies, Wilmington, DE), and lambda phage DNA (Promega) as the standard. .. Strain-specific forward primers for isolate 18U65 and 18D70 were designed from the nearly full-length 16S rRNA gene.

Marker:

Article Title: High-density Integrated Linkage Map Based on SSR Markers in Soybean
Article Snippet: Paragraph title: DNA extraction and marker analysis ... The concentration of DNA in solution was quantified by comparison with a serial dilution of lambda phage DNA (Promega, Madison, WI, USA) and was then adjusted to 10 ng/µL with sterile water.

Staining:

Article Title: Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels
Article Snippet: Permanent fusion bonding was achieved by heating the bonded substrate to 1000 °C and holding at this temperature for 48 h. After bonding, reservoirs were affixed over the powder blasted vias with a UV-curable epoxy to facilitate the introduction of solutions to the fluidic network. .. Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1. .. Solutions containing 0.5 ng μL−1 of DNA (16 pM for λ-phage and 4.6 pM for T4-phage) also contained 4% (by volume) β-mercaptoethanol (Fisher Scientific) to limit photo-induced damage and 2% (by mass) polyvinylpyrrolidone (PVP, 10 kDa, Sigma-Aldrich) to reduce electro-osmotic flow within the channels.

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    Promega unmethylated bacteriophage lambda dna
    Characterization of the Ae. aegypti methylome by whole-genome bisulfite sequencing. ( A ) Average methylation levels were determined for all cytosine residues and then distributed into bins with increasing methylation ratios. For comparison, the corresponding data is also shown for bacteriophage <t>lambda</t> (negative control) and human blood (positive control) <t>DNA</t> that was spiked into the Ae. aegypti sample prior to bisulfite conversion. The actual numerical values of the first bins are indicated above the corresponding bars. ( B ) Dinucleotide sequence context of unconverted cytosines. No unconverted cytosines were found in the lambda dataset.
    Unmethylated Bacteriophage Lambda Dna, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unmethylated bacteriophage lambda dna/product/Promega
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    unmethylated bacteriophage lambda dna - by Bioz Stars, 2019-10
    78/100 stars
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    Characterization of the Ae. aegypti methylome by whole-genome bisulfite sequencing. ( A ) Average methylation levels were determined for all cytosine residues and then distributed into bins with increasing methylation ratios. For comparison, the corresponding data is also shown for bacteriophage lambda (negative control) and human blood (positive control) DNA that was spiked into the Ae. aegypti sample prior to bisulfite conversion. The actual numerical values of the first bins are indicated above the corresponding bars. ( B ) Dinucleotide sequence context of unconverted cytosines. No unconverted cytosines were found in the lambda dataset.

    Journal: Scientific Reports

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome

    doi: 10.1038/srep36444

    Figure Lengend Snippet: Characterization of the Ae. aegypti methylome by whole-genome bisulfite sequencing. ( A ) Average methylation levels were determined for all cytosine residues and then distributed into bins with increasing methylation ratios. For comparison, the corresponding data is also shown for bacteriophage lambda (negative control) and human blood (positive control) DNA that was spiked into the Ae. aegypti sample prior to bisulfite conversion. The actual numerical values of the first bins are indicated above the corresponding bars. ( B ) Dinucleotide sequence context of unconverted cytosines. No unconverted cytosines were found in the lambda dataset.

    Article Snippet: As a spike-in control, unmethylated bacteriophage lambda DNA (Promega) and genomic DNA extracted from human blood (Blood & Cell Culture DNA Kit, Qiagen) were used.

    Techniques: Methylation Sequencing, Methylation, Negative Control, Positive Control, Sequencing