lambda dna  (new england biolabs)


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    Name:
    Lambda DNA
    Description:
    Lambda DNA 1 250 ug
    Catalog Number:
    N3011L
    Price:
    276
    Category:
    Genomic DNA
    Size:
    1 250 ug
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    Structured Review

    new england biolabs lambda dna
    Lambda DNA
    Lambda DNA 1 250 ug
    https://www.bioz.com/result/lambda dna/product/new england biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lambda dna - by Bioz Stars, 2021-06
    86/100 stars

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    Related Articles

    Purification:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    Agarose Gel Electrophoresis:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    Gel Extraction:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    In Vitro:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Article Title: Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging
    Article Snippet: We labeled the constitutive heterochromatin marker Histone H3K9me3 with the primary rabbit polyclonal antibody (ab8898, Abcam, Cambridge, England) and a secondary donkey anti-rabbit antibody (sc-362291, Santa Cruz Biotechnology, Dallas, TX). .. Preparation of DNA in vitro Solutions For the DNA in vitro solution experiments, double-stranded λ-DNA was obtained from New England Biolabs (Ipswich, MA). ..

    Methylation:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Lambda DNA Preparation:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

    Ligation:

    Article Title: Watching Individual Proteins Acting on Single Molecules of DNA
    Article Snippet: .. Bacteriophage λ DNA (New England Biolabs, Ipswich, MA) is biotinylated by ligation to a 3′-biotinylated 12-mer oligonucleotide (5′-GGGCGGCG ACCT-3′ or 5′-AGGTCGCCGCCC-3′, Operon Technologies, Huntsville, AL) that is complementary to one of the cohesive ends of λ DNA ( ). .. In all the subsequent protocols, the pipetting of solutions containing λ DNA should be performed with cut pipette tips to minimize shearing of the DNA.

    Staining:

    Article Title: Massively-Parallel Ultra-High-Aspect-Ratio Nanochannels as Mesoporous Membranes
    Article Snippet: .. The λ-DNA Hind digest (New England Biolabs) was stained with the intercalating fluorescence dye YOYO-1 (Invitrogen) at a dye-to-base pair ratio of ~1:10 in 5× TBE buffer. ..

    Fluorescence:

    Article Title: Massively-Parallel Ultra-High-Aspect-Ratio Nanochannels as Mesoporous Membranes
    Article Snippet: .. The λ-DNA Hind digest (New England Biolabs) was stained with the intercalating fluorescence dye YOYO-1 (Invitrogen) at a dye-to-base pair ratio of ~1:10 in 5× TBE buffer. ..

    Marker:

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

    Generated:

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

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  • 99
    New England Biolabs lambda phage dna
    Application of the real-time DOP-PCR to diverse <t>DNA</t> species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and <t>lambda</t> phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.
    Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda phage dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lambda phage dna - by Bioz Stars, 2021-06
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    99
    New England Biolabs cpg methylase sss i
    In vitro replication of <t>CpG-methylated</t> DNA. (A) Methylation status was determined after incubation of 200 ng of either unmethylated or Sss I-methylated pBSK − in DNA Xenopus egg extract for 30 min. Isolated and purified DNA was then subjected to restriction digestion with Cla I. N, nicked plasmid; S, supercoiled plasmid; L, linear plasmid. (B) Replication was determined after incubation of 25 ng of either unmethylated or Sss I-methylated lambda DNA in 10 μl of membrane-free Xenopus egg extract for 30 min, followed by the addition of 2.5 volumes of NPE. [α- 32 P]dATP was included in all replication assays at a concentration of 0.1 mCi of egg extract/μl. Then, 3-μl portions of the replication reaction products were visualized after being loaded onto a standard agarose gel. Time refers to number of minutes after the addition of the NPE. (C) Quantitation of replication of unmethylated (▪) and methylated (○) lambda DNA or a 2.9-kb plasmid, pBSK − , as determined by using ImageQuant software. (D) Quantitation of replication of increasing concentrations of pBSK − methylated plasmid after 60 min in NPE. DNA replication is expressed as the percent replication relative to replication of unmethylated plasmid at an equivalent concentration. (E) Quantitation of replication of an unmethylated 5.4-kb plasmid (pET-28a) in the absence (▪) or presence (▴) of the 2.9-kb pBSK − methylated plasmid (○).
    Cpg Methylase Sss I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cpg methylase sss i/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cpg methylase sss i - by Bioz Stars, 2021-06
    99/100 stars
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    Image Search Results


    Application of the real-time DOP-PCR to diverse DNA species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and lambda phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.

    Journal: PLoS ONE

    Article Title: Quantification of Trace-Level DNA by Real-Time Whole Genome Amplification

    doi: 10.1371/journal.pone.0028661

    Figure Lengend Snippet: Application of the real-time DOP-PCR to diverse DNA species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and lambda phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.

    Article Snippet: Concentrations of CTD, E. coli DNA, and lambda phage DNA were determined by measuring UV absorbance at 260 nm.

    Techniques: Degenerate Oligonucleotide–primed Polymerase Chain Reaction, Amplification

    Stretching of DNA molecules (a) near the inlet (micro to nano region) and (b) at about 2 mm away from the inlet of the nanochannel. The maximum elongation of λ-DNA reaches 10 μ m, which is about 50% of its fully extended length. (Scale bar: 20 μ m)

    Journal: Biomicrofluidics

    Article Title: Fabrication of long poly(dimethyl siloxane) nanochannels by replicating protein deposit from confined solution evaporation

    doi: 10.1063/1.4730371

    Figure Lengend Snippet: Stretching of DNA molecules (a) near the inlet (micro to nano region) and (b) at about 2 mm away from the inlet of the nanochannel. The maximum elongation of λ-DNA reaches 10 μ m, which is about 50% of its fully extended length. (Scale bar: 20 μ m)

    Article Snippet: Bovine serum albumin (BSA) was purchased from Sigma-Aldrich. λ-DNA was purchased from New England BioLab and labeled with YOYO-1 (Y3601, purchased from Invitrogen) for observation.

    Techniques:

    (a) Schematic of how a DNA chain is polarized, trapped or escape, and relax in HFM; (b) and (c) Time serial snapshots of the relaxation dynamics of an intermediated-stretched (b) and an over-stretched λ-DNA (c). (d) Extension versus residence

    Journal: Biomicrofluidics

    Article Title: Regulation of DNA conformations and dynamics in flows with hybrid field microfluidics

    doi: 10.1063/1.4762852

    Figure Lengend Snippet: (a) Schematic of how a DNA chain is polarized, trapped or escape, and relax in HFM; (b) and (c) Time serial snapshots of the relaxation dynamics of an intermediated-stretched (b) and an over-stretched λ-DNA (c). (d) Extension versus residence

    Article Snippet: A dilute solution (∼0.03 μ g/ml, about 10−4 of the overlapping concentration, at which the macromolecules completely fill the space but not overlapping) of λ-DNA (New England Biolab, Inc) in 10 mM TE buffer ( p H = 8.0) with 4% β-mercaptoethanol was used as the experimental fluid.

    Techniques:

    The ensemble average fractional extension of ∼200 λ-DNA when switching from “flow field” alone to “hybrid field” (a) and 60 λ-DNA molecules after the sudden removal of the electric field (c). (b)

    Journal: Biomicrofluidics

    Article Title: Regulation of DNA conformations and dynamics in flows with hybrid field microfluidics

    doi: 10.1063/1.4762852

    Figure Lengend Snippet: The ensemble average fractional extension of ∼200 λ-DNA when switching from “flow field” alone to “hybrid field” (a) and 60 λ-DNA molecules after the sudden removal of the electric field (c). (b)

    Article Snippet: A dilute solution (∼0.03 μ g/ml, about 10−4 of the overlapping concentration, at which the macromolecules completely fill the space but not overlapping) of λ-DNA (New England Biolab, Inc) in 10 mM TE buffer ( p H = 8.0) with 4% β-mercaptoethanol was used as the experimental fluid.

    Techniques:

    (a) Schematic of the working principles of HFM, (b) a typical application scheme for an electric bias in HFM, (c) regulation the conformations and dynamics (trapping, concentration, and sudden stretching) of λ-DNA molecules in HFM, and (d) schematic

    Journal: Biomicrofluidics

    Article Title: Regulation of DNA conformations and dynamics in flows with hybrid field microfluidics

    doi: 10.1063/1.4762852

    Figure Lengend Snippet: (a) Schematic of the working principles of HFM, (b) a typical application scheme for an electric bias in HFM, (c) regulation the conformations and dynamics (trapping, concentration, and sudden stretching) of λ-DNA molecules in HFM, and (d) schematic

    Article Snippet: A dilute solution (∼0.03 μ g/ml, about 10−4 of the overlapping concentration, at which the macromolecules completely fill the space but not overlapping) of λ-DNA (New England Biolab, Inc) in 10 mM TE buffer ( p H = 8.0) with 4% β-mercaptoethanol was used as the experimental fluid.

    Techniques: Concentration Assay

    In vitro replication of CpG-methylated DNA. (A) Methylation status was determined after incubation of 200 ng of either unmethylated or Sss I-methylated pBSK − in DNA Xenopus egg extract for 30 min. Isolated and purified DNA was then subjected to restriction digestion with Cla I. N, nicked plasmid; S, supercoiled plasmid; L, linear plasmid. (B) Replication was determined after incubation of 25 ng of either unmethylated or Sss I-methylated lambda DNA in 10 μl of membrane-free Xenopus egg extract for 30 min, followed by the addition of 2.5 volumes of NPE. [α- 32 P]dATP was included in all replication assays at a concentration of 0.1 mCi of egg extract/μl. Then, 3-μl portions of the replication reaction products were visualized after being loaded onto a standard agarose gel. Time refers to number of minutes after the addition of the NPE. (C) Quantitation of replication of unmethylated (▪) and methylated (○) lambda DNA or a 2.9-kb plasmid, pBSK − , as determined by using ImageQuant software. (D) Quantitation of replication of increasing concentrations of pBSK − methylated plasmid after 60 min in NPE. DNA replication is expressed as the percent replication relative to replication of unmethylated plasmid at an equivalent concentration. (E) Quantitation of replication of an unmethylated 5.4-kb plasmid (pET-28a) in the absence (▪) or presence (▴) of the 2.9-kb pBSK − methylated plasmid (○).

    Journal: Molecular and Cellular Biology

    Article Title: CpG Methylation of DNA Restricts Prereplication Complex Assembly in Xenopus Egg Extracts

    doi: 10.1128/MCB.23.19.6769-6779.2003

    Figure Lengend Snippet: In vitro replication of CpG-methylated DNA. (A) Methylation status was determined after incubation of 200 ng of either unmethylated or Sss I-methylated pBSK − in DNA Xenopus egg extract for 30 min. Isolated and purified DNA was then subjected to restriction digestion with Cla I. N, nicked plasmid; S, supercoiled plasmid; L, linear plasmid. (B) Replication was determined after incubation of 25 ng of either unmethylated or Sss I-methylated lambda DNA in 10 μl of membrane-free Xenopus egg extract for 30 min, followed by the addition of 2.5 volumes of NPE. [α- 32 P]dATP was included in all replication assays at a concentration of 0.1 mCi of egg extract/μl. Then, 3-μl portions of the replication reaction products were visualized after being loaded onto a standard agarose gel. Time refers to number of minutes after the addition of the NPE. (C) Quantitation of replication of unmethylated (▪) and methylated (○) lambda DNA or a 2.9-kb plasmid, pBSK − , as determined by using ImageQuant software. (D) Quantitation of replication of increasing concentrations of pBSK − methylated plasmid after 60 min in NPE. DNA replication is expressed as the percent replication relative to replication of unmethylated plasmid at an equivalent concentration. (E) Quantitation of replication of an unmethylated 5.4-kb plasmid (pET-28a) in the absence (▪) or presence (▴) of the 2.9-kb pBSK − methylated plasmid (○).

    Article Snippet: Plasmid and lambda DNA were methylated in vitro by using the bacterial CpG methylase Sss I (NEB, Beverly, Mass.) according to manufacturer's instructions.

    Techniques: In Vitro, Methylation, Incubation, Isolation, Purification, Plasmid Preparation, Lambda DNA Preparation, Concentration Assay, Agarose Gel Electrophoresis, Quantitation Assay, Software, Positron Emission Tomography