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Difco
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Condalab
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Difco
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Cell Signaling Technology Inc
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Neogen
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Journal: iScience
Article Title: Newly isolated human-derived Lactobacillus fermentum strain stimulates IFN-γ-secreting CD8 + T cells for enhanced anti-cancer immunity
doi: 10.1016/j.isci.2026.115642
Figure Lengend Snippet: Heat-killed MI37 stimulates IFN-γ production in CD8 + T cells (A) IFN-γ levels in culture supernatants of splenocytes treated with vehicle, De Man, Rogosa, and Sharpe (MRS) broth, MI37-CFCS, or by KCTC3112-CFCS (KCTC-CFCS) in the presence of 500 ng/mL LPS were measured by ELISA. (B) IFN-γ ELISA of splenocytes treated with viable or heat-killed (HK) bacteria. (C) Representative FACS plots and bar graphs showing the frequency (%) of CD8 + IFN-γ + T cells in splenocytes treated with viable or HK bacteria. (D) Tumor growth in MC38 tumor-bearing mice. (E) IFN-γ levels measured by ELISA in tumor homogenates from MC38 tumor-bearing mice. Data are presented as mean ± SD. Statistical significance was determined using one-way or two-way ANOVA with multiple comparisons. Data are representative of two independent experiments. ∗ p < 0.05 , ∗∗ p < 0.01, ∗∗∗ p < 0.001 .
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Bacteria