lactamase activity  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Millipore lactamase activity
    Reporter assay for detection of AmpG permease activity. The <t>β-lactamase</t> assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.
    Lactamase Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactamase activity/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lactamase activity - by Bioz Stars, 2020-08
    91/100 stars

    Images

    1) Product Images from "Regulation and Molecular Basis of Environmental Muropeptide Uptake and Utilization in Fastidious Oral Anaerobe Tannerella forsythia"

    Article Title: Regulation and Molecular Basis of Environmental Muropeptide Uptake and Utilization in Fastidious Oral Anaerobe Tannerella forsythia

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.00648

    Reporter assay for detection of AmpG permease activity. The β-lactamase assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.
    Figure Legend Snippet: Reporter assay for detection of AmpG permease activity. The β-lactamase assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.

    Techniques Used: Reporter Assay, Activity Assay, Beta Lactamase Assay, Plasmid Preparation

    2) Product Images from "E. coli Selection of Human Genes Encoding Secreted and Membrane Proteins Based on cDNA Fusions to a Leaderless ?-Lactamase Reporter"

    Article Title: E. coli Selection of Human Genes Encoding Secreted and Membrane Proteins Based on cDNA Fusions to a Leaderless ?-Lactamase Reporter

    Journal: Genome Research

    doi: 10.1101/gr.1000903

    ( A ) The arrangement of E. coli and mammalian promoters in relation to the cDNA and leaderless β -lactamase gene. ( B ) The sequences at the multiple cloning site of pBK-CMV-fusion where the cDNA fuses with the leaderless β -lactamase gene.
    Figure Legend Snippet: ( A ) The arrangement of E. coli and mammalian promoters in relation to the cDNA and leaderless β -lactamase gene. ( B ) The sequences at the multiple cloning site of pBK-CMV-fusion where the cDNA fuses with the leaderless β -lactamase gene.

    Techniques Used: Clone Assay

    Related Articles

    Activity Assay:

    Article Title: Regulation and Molecular Basis of Environmental Muropeptide Uptake and Utilization in Fastidious Oral Anaerobe Tannerella forsythia
    Article Snippet: .. Lactamase activity of each strain lysate was assayed using the chromogenic nitrocefin substrate (Calbiochem). ..

    Article Title: E. coli Selection of Human Genes Encoding Secreted and Membrane Proteins Based on cDNA Fusions to a Leaderless ?-Lactamase Reporter
    Article Snippet: .. Lactamase activity was determined by measuring hydrolysis of the chromogenic substrate nitrocefin (Calbiochem; ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore β lactamase activity
    Immobilization of enzymes on the surface of lactococcal GEM particles. (a) Relative α-amylase activities on GEM particles incubated with culture medium containing soluble AmyL, PA (PA3), or α-PA. (b) Relative α-amylase and <t>β-lactamase</t>
    β Lactamase Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β lactamase activity/product/Millipore
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    β lactamase activity - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    91
    Millipore lactamase activity
    Reporter assay for detection of AmpG permease activity. The <t>β-lactamase</t> assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.
    Lactamase Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactamase activity/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lactamase activity - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Immobilization of enzymes on the surface of lactococcal GEM particles. (a) Relative α-amylase activities on GEM particles incubated with culture medium containing soluble AmyL, PA (PA3), or α-PA. (b) Relative α-amylase and β-lactamase

    Journal: Applied and Environmental Microbiology

    Article Title: Novel Surface Display System for Proteins on Non-Genetically Modified Gram-Positive Bacteria

    doi: 10.1128/AEM.72.1.880-889.2006

    Figure Lengend Snippet: Immobilization of enzymes on the surface of lactococcal GEM particles. (a) Relative α-amylase activities on GEM particles incubated with culture medium containing soluble AmyL, PA (PA3), or α-PA. (b) Relative α-amylase and β-lactamase

    Article Snippet: After 60 min, GEM particles and insoluble amylose azure were spun down, and the absorbance at 595 nm was determined. β-Lactamase activity was measured by adding 40 μl nitrocefin (CalBiochem) to GEM particles loaded with β-PA in 1 ml (final volume) of PBS.

    Techniques: Incubation

    Inactivation of lasR confers increased β-lactamase activity and β-lactam tolerance. A. Ceftazidime-resistant colonies emerged in a lawn of CF416 or CF416L1 cells on LB agar with 20 µg ml −1 of ceftazidime (Petri dish upper and lower halves), but only the lasR mutant lawns yielded small partially resistant colonies whose growth is inhibited by the addition of 8 µg ml −1 of the β-lactamase inhibitor tazobactam (Petri dish lower halves). Photographs were taken after incubation overnight at 37°C followed by 3 days at 22°C. B. β-Lactamase activity in culture supernatants, measured as a change in optical density at 490 nm (ΔA 490 ) after addition of the chromogenic substrate nitrocefin. Cases with no change are marked with an asterisk. Values are the average of three technical replicates, and error bars show standard deviations. Equivalent results were obtained using a qualitative whole-culture assay. C. β-Lactamase activity in whole cultures of CF416 and CF416 lasR ::Gm. Values are the averages of three cultures, and error bars show standard error of the mean. One unit of β-lactamase is defined as 1 µmol of nitrocefin hydrolysed per min per mg of total protein. D. Reduced killing by ceftazidime of the lasR mutant CF416L1 relative to CF416 and CF416L1R. Values are the average cfu of two cultures, and error bars show standard deviations.

    Journal: Molecular microbiology

    Article Title: Growth phenotypes of Pseudomonas aeruginosa lasR mutants adapted to the airways of cystic fibrosis patients

    doi: 10.1111/j.1365-2958.2007.05678.x

    Figure Lengend Snippet: Inactivation of lasR confers increased β-lactamase activity and β-lactam tolerance. A. Ceftazidime-resistant colonies emerged in a lawn of CF416 or CF416L1 cells on LB agar with 20 µg ml −1 of ceftazidime (Petri dish upper and lower halves), but only the lasR mutant lawns yielded small partially resistant colonies whose growth is inhibited by the addition of 8 µg ml −1 of the β-lactamase inhibitor tazobactam (Petri dish lower halves). Photographs were taken after incubation overnight at 37°C followed by 3 days at 22°C. B. β-Lactamase activity in culture supernatants, measured as a change in optical density at 490 nm (ΔA 490 ) after addition of the chromogenic substrate nitrocefin. Cases with no change are marked with an asterisk. Values are the average of three technical replicates, and error bars show standard deviations. Equivalent results were obtained using a qualitative whole-culture assay. C. β-Lactamase activity in whole cultures of CF416 and CF416 lasR ::Gm. Values are the averages of three cultures, and error bars show standard error of the mean. One unit of β-lactamase is defined as 1 µmol of nitrocefin hydrolysed per min per mg of total protein. D. Reduced killing by ceftazidime of the lasR mutant CF416L1 relative to CF416 and CF416L1R. Values are the average cfu of two cultures, and error bars show standard deviations.

    Article Snippet: To assay β-lactamase activity in culture supernatants, P. aeruginosa cultures grown overnight with LB broth were passed through a filter (0.22 µm pore size, Millipore), and the cell-free supernatants were assayed for β-lactamase activity 30 min after addition of nitrocefin (Calbiochem), as described by the manufacturer, except that A490 was measured, and corrected by subtracting the value measured for the supernatant without nitrocefin.

    Techniques: Activity Assay, Mutagenesis, Incubation

    β-Lactamase-Vpr entry assay. Viruses were produced by cotransfection of the indicated provirus and pMM310, a β-lactamase-Vpr (BlaM-Vpr) fusion protein expression vector. Viruses were pelleted, resuspended in 1 ml of medium, normalized by p24 capsid concentration, and then used to infect P4.R5 cells for 5 h. After washing, cells were incubated with the β-lactamase-sensitive fluorochrome CCF2/AM for 16 to 18 h at 25°C. Analysis was performed by flow cytometry. Uncleaved substrate was detected as green fluorescence, and cleaved substrate was detected as blue fluorescence. (A) Plot of green fluorescence versus blue fluorescence. Loaded cell control refers to uninfected cells loaded with CCF2/AM. Box R3 encloses cells used for the ratio analysis in panel B, cells positive for CCF2/AM loading. (B) Data are expressed as a ratio of blue to green fluorescence versus side scatter and are gated using uninfected cells (loaded cell control). AMD3100 (100 nm) is a CXCR4 antagonist used to block entry into cells.

    Journal: Journal of Virology

    Article Title: The Membrane-Proximal Tyrosine-Based Sorting Signal of Human Immunodeficiency Virus Type 1 gp41 Is Required for Optimal Viral Infectivity

    doi: 10.1128/JVI.78.3.1069-1079.2004

    Figure Lengend Snippet: β-Lactamase-Vpr entry assay. Viruses were produced by cotransfection of the indicated provirus and pMM310, a β-lactamase-Vpr (BlaM-Vpr) fusion protein expression vector. Viruses were pelleted, resuspended in 1 ml of medium, normalized by p24 capsid concentration, and then used to infect P4.R5 cells for 5 h. After washing, cells were incubated with the β-lactamase-sensitive fluorochrome CCF2/AM for 16 to 18 h at 25°C. Analysis was performed by flow cytometry. Uncleaved substrate was detected as green fluorescence, and cleaved substrate was detected as blue fluorescence. (A) Plot of green fluorescence versus blue fluorescence. Loaded cell control refers to uninfected cells loaded with CCF2/AM. Box R3 encloses cells used for the ratio analysis in panel B, cells positive for CCF2/AM loading. (B) Data are expressed as a ratio of blue to green fluorescence versus side scatter and are gated using uninfected cells (loaded cell control). AMD3100 (100 nm) is a CXCR4 antagonist used to block entry into cells.

    Article Snippet: To visualize β-lactamase activity, 10 μl of 1 mM nitrocefin (Calbiochem, San Diego, Calif.) was added, and the solution was incubated in the dark at room temperature for 22 h. A color change from yellow to red was detected in a microplate spectrophotometer using a 490-nm filter.

    Techniques: Produced, Cotransfection, Expressing, Plasmid Preparation, Concentration Assay, Incubation, Flow Cytometry, Cytometry, Fluorescence, Blocking Assay

    Reporter assay for detection of AmpG permease activity. The β-lactamase assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.

    Journal: Frontiers in Microbiology

    Article Title: Regulation and Molecular Basis of Environmental Muropeptide Uptake and Utilization in Fastidious Oral Anaerobe Tannerella forsythia

    doi: 10.3389/fmicb.2017.00648

    Figure Lengend Snippet: Reporter assay for detection of AmpG permease activity. The β-lactamase assay was performed on Escherichia coli strains BW25113 (parental strain), TP73 (Δ ampD )/pNU305, AR74 (Δ ampG/ Δ ampD )/pNU305/ pACY-AR1 (empty vector), and AR74/pNU305/pAC-Tanf_08635. Activity was determined by chromogenic nitrocefin substrate induced at 0, 30, 60 min and absorbance was measured at OD 486 . Data are representative of three independent experiments with similar results. Each value represents the mean (±SD) of three values measured in one representative assay.

    Article Snippet: Lactamase activity of each strain lysate was assayed using the chromogenic nitrocefin substrate (Calbiochem).

    Techniques: Reporter Assay, Activity Assay, Beta Lactamase Assay, Plasmid Preparation