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Agilent technologies laboratory chip device
Laboratory Chip Device, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laboratory chip device/product/Agilent technologies
Average 85 stars, based on 1 article reviews
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laboratory chip device - by Bioz Stars, 2020-05
85/100 stars

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real-time taqman pcr assay
conventional thermal cycler

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Polymerase Chain Reaction:

Article Title: Deployable Laboratory Response to Emergence of Melioidosis in Central Sri Lanka ▿
Article Snippet: .. The methods used were developed using laboratory equipment that was sufficiently robust to operate in a severe environment, namely, a real-time TaqMan PCR assay developed in our central laboratory ( ) but with amplification using a conventional thermal cycler and resolution by capillary electrophoresis on a laboratory chip device (Bioanalyzer 2100; Agilent Technologies, Little Falls, DE). .. Suspected Burkholderia pseudomallei isolates were obtained from patients with gram-negative septicemia seen in the University Hospital in Peradeniya.

Electrophoresis:

Article Title: Deployable Laboratory Response to Emergence of Melioidosis in Central Sri Lanka ▿
Article Snippet: .. The methods used were developed using laboratory equipment that was sufficiently robust to operate in a severe environment, namely, a real-time TaqMan PCR assay developed in our central laboratory ( ) but with amplification using a conventional thermal cycler and resolution by capillary electrophoresis on a laboratory chip device (Bioanalyzer 2100; Agilent Technologies, Little Falls, DE). .. Suspected Burkholderia pseudomallei isolates were obtained from patients with gram-negative septicemia seen in the University Hospital in Peradeniya.

Amplification:

Article Title: Deployable Laboratory Response to Emergence of Melioidosis in Central Sri Lanka ▿
Article Snippet: .. The methods used were developed using laboratory equipment that was sufficiently robust to operate in a severe environment, namely, a real-time TaqMan PCR assay developed in our central laboratory ( ) but with amplification using a conventional thermal cycler and resolution by capillary electrophoresis on a laboratory chip device (Bioanalyzer 2100; Agilent Technologies, Little Falls, DE). .. Suspected Burkholderia pseudomallei isolates were obtained from patients with gram-negative septicemia seen in the University Hospital in Peradeniya.

Chromatin Immunoprecipitation:

Article Title: Deployable Laboratory Response to Emergence of Melioidosis in Central Sri Lanka ▿
Article Snippet: .. The methods used were developed using laboratory equipment that was sufficiently robust to operate in a severe environment, namely, a real-time TaqMan PCR assay developed in our central laboratory ( ) but with amplification using a conventional thermal cycler and resolution by capillary electrophoresis on a laboratory chip device (Bioanalyzer 2100; Agilent Technologies, Little Falls, DE). .. Suspected Burkholderia pseudomallei isolates were obtained from patients with gram-negative septicemia seen in the University Hospital in Peradeniya.

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  • 86
    Agilent technologies dna microarray analysis a rat
    Workflow from sampling and grinding of the hippocampus, total RNA extraction, and <t>DNA</t> <t>microarray</t> analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).
    Dna Microarray Analysis A Rat, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray analysis a rat/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna microarray analysis a rat - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    85
    Agilent technologies whole mouse genome microarray analysis
    Genome-wide expression analysis of IL-1R-regulated genes. (A) Schematic diagram of the <t>microarray</t> design used in this study. WT or Il - 1r −/− BMDCs were mock infected or infected with WNV at an MOI of 2.5. Total RNA was extracted at 24 and 48 h p.i. and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant change is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of
    Whole Mouse Genome Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole mouse genome microarray analysis/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    whole mouse genome microarray analysis - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    93
    Agilent technologies dna microarray analysis
    The experimental strategy to screen for genes that are influenced by lavender oil (LO). Rats (7 weeks old) were given lavender oil (LO) orally once each day for 13 days, and on the 14 th day, the small intestine, spleen and liver were dissected out and deep frozen in liquid nitrogen. <t>DNA</t> <t>microarray</t> analysis was performed using a rat whole-genome DNA microarray chip (G4131F, Agilent), and the differentially expressed genes were identified in the LO-treated tissues followed by bioinformatics analyses. For further details, see the Materials and Methods section.
    Dna Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray analysis/product/Agilent technologies
    Average 93 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    dna microarray analysis - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

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    Workflow from sampling and grinding of the hippocampus, total RNA extraction, and DNA microarray analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).

    Journal: Physiological Reports

    Article Title: DNA microarray‐based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity

    doi: 10.14814/phy2.12206

    Figure Lengend Snippet: Workflow from sampling and grinding of the hippocampus, total RNA extraction, and DNA microarray analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).

    Article Snippet: DNA microarray analysis A rat 4 × 44K whole genome oligo DNA microarray chip (G4131F, Agilent Technologies, Palo Alto, CA) was used for global gene expression analysis using the hippocampi.

    Techniques: Sampling, RNA Extraction, Microarray, Spectrophotometry, Agarose Gel Electrophoresis, Chromatin Immunoprecipitation

    Genome-wide expression analysis of IL-1R-regulated genes. (A) Schematic diagram of the microarray design used in this study. WT or Il - 1r −/− BMDCs were mock infected or infected with WNV at an MOI of 2.5. Total RNA was extracted at 24 and 48 h p.i. and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant change is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Journal: mBio

    Article Title: Interleukin-1β Signaling in Dendritic Cells Induces Antiviral Interferon Responses

    doi: 10.1128/mBio.00342-18

    Figure Lengend Snippet: Genome-wide expression analysis of IL-1R-regulated genes. (A) Schematic diagram of the microarray design used in this study. WT or Il - 1r −/− BMDCs were mock infected or infected with WNV at an MOI of 2.5. Total RNA was extracted at 24 and 48 h p.i. and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant change is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Article Snippet: Total RNA was harvested at 24 and 48 h p.i., and relative gene expression levels were determined by Agilent Whole Mouse Genome Microarray analysis (4×44K chip).

    Techniques: Genome Wide, Expressing, Microarray, Infection

    IL-1β treatment drives expression of IFN-β and ISGs. (A) Schematic diagram of the microarray design used in this study. WT BMDCs were mock treated or treated with IL-1β (100 ng/ml). Total RNA was extracted at 24 and 48 h posttreatment and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant changes is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Journal: mBio

    Article Title: Interleukin-1β Signaling in Dendritic Cells Induces Antiviral Interferon Responses

    doi: 10.1128/mBio.00342-18

    Figure Lengend Snippet: IL-1β treatment drives expression of IFN-β and ISGs. (A) Schematic diagram of the microarray design used in this study. WT BMDCs were mock treated or treated with IL-1β (100 ng/ml). Total RNA was extracted at 24 and 48 h posttreatment and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant changes is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Article Snippet: Total RNA was harvested at 24 and 48 h p.i., and relative gene expression levels were determined by Agilent Whole Mouse Genome Microarray analysis (4×44K chip).

    Techniques: Expressing, Microarray

    Genome-wide expression analysis of IL-1R-regulated genes. (A) Schematic diagram of the microarray design used in this study. WT or Il - 1r −/− BMDCs were mock infected or infected with WNV at an MOI of 2.5. Total RNA was extracted at 24 and 48 h p.i. and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant change is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Journal: mBio

    Article Title: Interleukin-1β Signaling in Dendritic Cells Induces Antiviral Interferon Responses

    doi: 10.1128/mBio.00342-18

    Figure Lengend Snippet: Genome-wide expression analysis of IL-1R-regulated genes. (A) Schematic diagram of the microarray design used in this study. WT or Il - 1r −/− BMDCs were mock infected or infected with WNV at an MOI of 2.5. Total RNA was extracted at 24 and 48 h p.i. and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant change is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Article Snippet: Total RNA was harvested at 24 and 48 h p.i., and relative gene expression levels were determined by Agilent Whole Mouse Genome Microarray analysis (4×44K chip).

    Techniques: Genome Wide, Expressing, Microarray, Infection

    IL-1β treatment drives expression of IFN-β and ISGs. (A) Schematic diagram of the microarray design used in this study. WT BMDCs were mock treated or treated with IL-1β (100 ng/ml). Total RNA was extracted at 24 and 48 h posttreatment and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant changes is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Journal: mBio

    Article Title: Interleukin-1β Signaling in Dendritic Cells Induces Antiviral Interferon Responses

    doi: 10.1128/mBio.00342-18

    Figure Lengend Snippet: IL-1β treatment drives expression of IFN-β and ISGs. (A) Schematic diagram of the microarray design used in this study. WT BMDCs were mock treated or treated with IL-1β (100 ng/ml). Total RNA was extracted at 24 and 48 h posttreatment and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant changes is defined as a > 1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of

    Article Snippet: Total RNA was harvested at 24 and 48 h p.i., and relative gene expression levels were determined by Agilent Whole Mouse Genome Microarray analysis (4×44K chip).

    Techniques: Expressing, Microarray

    The experimental strategy to screen for genes that are influenced by lavender oil (LO). Rats (7 weeks old) were given lavender oil (LO) orally once each day for 13 days, and on the 14 th day, the small intestine, spleen and liver were dissected out and deep frozen in liquid nitrogen. DNA microarray analysis was performed using a rat whole-genome DNA microarray chip (G4131F, Agilent), and the differentially expressed genes were identified in the LO-treated tissues followed by bioinformatics analyses. For further details, see the Materials and Methods section.

    Journal: PLoS ONE

    Article Title: Unraveling the Rat Intestine, Spleen and Liver Genome-Wide Transcriptome after the Oral Administration of Lavender Oil by a Two-Color Dye-Swap DNA Microarray Approach

    doi: 10.1371/journal.pone.0129951

    Figure Lengend Snippet: The experimental strategy to screen for genes that are influenced by lavender oil (LO). Rats (7 weeks old) were given lavender oil (LO) orally once each day for 13 days, and on the 14 th day, the small intestine, spleen and liver were dissected out and deep frozen in liquid nitrogen. DNA microarray analysis was performed using a rat whole-genome DNA microarray chip (G4131F, Agilent), and the differentially expressed genes were identified in the LO-treated tissues followed by bioinformatics analyses. For further details, see the Materials and Methods section.

    Article Snippet: Global gene expression analysis on a rat whole-genome DNA chip The total RNA that was extracted from the small intestine, spleen, and liver tissues for each control and LO-administered sample was pooled in each group prior to DNA microarray analysis (Rat whole genome 4×44-K:G4131F; Agilent Technologies, Palo Alto, CA, USA).

    Techniques: Microarray, Chromatin Immunoprecipitation