Structured Review

Agilent technologies lab on a chip technology
Lab On A Chip Technology, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lab on a chip technology/product/Agilent technologies
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
lab on a chip technology - by Bioz Stars, 2019-10
94/100 stars

Images

Related Articles

Amplification:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA). .. After cDNA purification, in vitro transcription reaction resulted in aRNA, which was also purified.

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: A database search with BLASTN (NCBI) revealed complete and highly significant matches for both primers and probe only for the IFNL4 RNAs (E = 0.007 for forward and reverse primers each, and E = 0.85 for the probe). .. The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany). .. The sequence of the amplicon was confirmed by automated custom sequencing (SeqLab Goettingen, Germany).

Mass Spectrometry:

Article Title: Copper-Deficiency in Brassica napus Induces Copper Remobilization, Molybdenum Accumulation and Modification of the Expression of Chloroplastic Proteins
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent). .. The ESI LC–MS/MS data were converted into DTA– format files that were further searched for proteins with MASCOT Daemon (Matrix Science ).

Article Title: A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
Article Snippet: Peptides were then dried and dissolved in starting buffer (3% CH3 CN and 0.1% HCOOH, w /v ) for chromatographic elution. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The ESI LC-MS/MSdata were converted into DTA-format files that were further searched for protein identification with MASCOT Daemon (Matrix Science, [ ]) in the NCBInr-protein sequence database, Viridiplantae (green plants), and in the Brassica EST database (Brassica Genome Gateway 2007, [ ]).

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: Peptide extracts were then dried and dissolved in a starting buffer, made up of 3% CH3 CN and 0.1% HCOOH in water, for chromatographic elution. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: Peptide extracts were dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, MA, USA).

Article Title: Does ear C sink strength contribute to overcoming photosynthetic acclimation of wheat plants exposed to elevated CO2?
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, which consisted of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4; Bruker Daltonic, Billerica, MA, USA).

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.)
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, which consisted of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the DataAnalysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: Evidence for Proteomic and Metabolic Adaptations Associated with Alterations of Seed Yield and Quality in Sulfur-limited Brassica napu
Article Snippet: Peptide extracts were dried and dissolved in starting buffer for chromatographic elution; the buffer consisted of 3% CH3 CN and 0.1% HCOOH. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The software used to generate the peak list was Data Analysis for 6300 series Ion Trap LC/MS (v3.4; Bruker Daltonique, Wissembourg, France).

Cytometry:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: The purity of the isolated monocyte population was on average 90% as determined by CD14+ -staining on flow cytometry analysis. mRNA was isolated from cell collections using Trizol reagent (Invitrogen/Gibco, CA) according to the manufacturer's instruction. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Enzyme-linked Immunosorbent Assay:

Article Title: Oxidative Damage in Clinical Ischemia/Reperfusion Injury: A Reappraisal
Article Snippet: Next, activated Nrf2 in cell lysates was quantified by DNA-binding ELISA according to the manufacturers' instructions (TransAM Nrf2; Active Motif). .. For expression profiling, the integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (Agilent Technologies).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix). .. CREB (Calbiochem), phosphorylated CREB (R & D Systems), and β-actin (R & D Systems) antibodies were used to detect respective proteins by Western blotting on cell lysates obtained from hMSCs treated with various supplements.

Real-time Polymerase Chain Reaction:

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Paragraph title: Gene Expression Analysis by qPCR and Microarray. ... The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Microarray:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: Paragraph title: Sample collection and microarray procedures ... In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Article Title: Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis
Article Snippet: The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation
Article Snippet: The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Amstelveen, Netherlands). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands and displayed no chromosomal peaks or RNA degradation products (RNA integrity number > 8.0).

Article Title: Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress
Article Snippet: Paragraph title: Microarray experiments ... The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: Microdissection was conducted using the PixCell IIe Laser Capture Microdissection system (Molecular Devices Corporation, Sunnyvale, CA), which directs the low-power infrared laser through the cap to melt the film onto the cells of interest, which were then lifted from the tissues section RNA was extracted from whole tissue specimens as well as LCM treated samples at the Microarray Core Facility of the Texas Medical Center Digestive Disease Center at Baylor College of Medicine using the Qiagen RNeasy Extraction kit (Qiagen, Inc, Valencia, CA, USA). .. The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA).

Article Title: Oxidative Damage in Clinical Ischemia/Reperfusion Injury: A Reappraisal
Article Snippet: For expression profiling, the integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (Agilent Technologies). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0) ( ).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Paragraph title: Gene Expression Analysis by qPCR and Microarray. ... The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Incubation:

Article Title: Copper-Deficiency in Brassica napus Induces Copper Remobilization, Molybdenum Accumulation and Modification of the Expression of Chloroplastic Proteins
Article Snippet: The gel fragments were subsequently incubated twice for 15 min in a 0.1% CH3 CN solution in water to allow extraction of peptides from the gel pieces. .. Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent).

Article Title: A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
Article Snippet: The resulting peptides were extracted from the gel by two incubation periods of 15 min in a 0.1% CH3 CN solution (w /v ). .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: Gel fragments were subsequently incubated twice for 15min in a H2 O/CH3 CN solution to allow the extraction of peptides. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent).

Article Title: The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Article Snippet: For RNA isolation, 2.5 ml blood was drawn in PAXgene tubes, incubated for two hours at room temperature and stored at -20°C. .. Total RNA concentration was measured using the Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA) and RNA purity and integrity was verified using lab-on-chip technology (Agilent 2100 Bioanalyzer, Santa Clara, CA, USA).

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: The gel fragments were subsequently incubated twice for 15 min in a H2 O/CH3 CN solution to allow peptide extraction from the gel pieces. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Does ear C sink strength contribute to overcoming photosynthetic acclimation of wheat plants exposed to elevated CO2?
Article Snippet: The gel fragments were subsequently incubated twice for 15 min in a H2 O/CH3 CN solution to allow extraction of peptides from the gel pieces. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: The gel fragments were subsequently incubated twice for 15 min in a H2 O/CH3 CN solution to allow extraction of peptides from the gel pieces. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Evidence for Proteomic and Metabolic Adaptations Associated with Alterations of Seed Yield and Quality in Sulfur-limited Brassica napu
Article Snippet: The gel fragments were subsequently incubated twice for 15 min in a 0.1% CH3 CN solution to allow extraction of peptides from the gel pieces. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent) and fragmented using an on-line XCT mass spectrometer (Agilent).

Activity Assay:

Article Title: A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
Article Snippet: Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

In Silico:

Article Title: Novel Insights into miRNA in Lung and Heart Inflammatory Diseases
Article Snippet: Among the few possibilities, 2100 Bioanalyzer, a lab on-chip technology from Agilent Technologies, offers both qualitative and quantitative estimation of miRNAs. .. Among the few possibilities, 2100 Bioanalyzer, a lab on-chip technology from Agilent Technologies, offers both qualitative and quantitative estimation of miRNAs.

Expressing:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA). .. After cDNA purification, in vitro transcription reaction resulted in aRNA, which was also purified.

Article Title: Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis
Article Snippet: Paragraph title: Nucleic acid extraction and gene expression analysis ... The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation
Article Snippet: The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Amstelveen, Netherlands). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands and displayed no chromosomal peaks or RNA degradation products (RNA integrity number > 8.0).

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: Paragraph title: Quantification of hepatic gene expression ... The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Article Title: Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress
Article Snippet: The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. Microarrays were carried out by the Shanghai Biotechnology Corporation using the One-Cycle Target Labeling kits and Affymetrix GeneChips, following manufacturers’ instructions.

Article Title: Oxidative Damage in Clinical Ischemia/Reperfusion Injury: A Reappraisal
Article Snippet: Total RNA was extracted from renal or myocardial tissues as described earlier, using GAPDH as internal control ( ). .. For expression profiling, the integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (Agilent Technologies). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0) ( ).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Paragraph title: Gene Expression Analysis by qPCR and Microarray. ... The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Genome Wide:

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: To study the genome-wide effect of db-cAMP, hMSCs were grown in either basic medium or basic medium supplemented with 1 mM db-cAMP for 6 h or 7 days. .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Transplantation Assay:

Article Title: Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation
Article Snippet: Paired biopsies were taken for the patients undergoing renal transplantation with one biopsy prior to transplantation and one biopsy 45 minutes after transplant reperfusion. .. The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Amstelveen, Netherlands).

Western Blot:

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix). .. Data analysis was performed by using Affymetrix GENECHIP 4.0 software.

Hybridization:

Article Title: Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis
Article Snippet: The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: Effect of body fat distribution on the transcription response to dietary fat interventions
Article Snippet: Paragraph title: RNA isolation, labeling and hybridization ... The integrity of RNA obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Flow Cytometry:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: The purity of the isolated monocyte population was on average 90% as determined by CD14+ -staining on flow cytometry analysis. mRNA was isolated from cell collections using Trizol reagent (Invitrogen/Gibco, CA) according to the manufacturer's instruction. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Lab-on-a-Chip:

Article Title: Copper-Deficiency in Brassica napus Induces Copper Remobilization, Molybdenum Accumulation and Modification of the Expression of Chloroplastic Proteins
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent). .. The ESI LC–MS/MS data were converted into DTA– format files that were further searched for proteins with MASCOT Daemon (Matrix Science ).

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: Subsequently, mRNA was purified with NucleoSpin® RNAII (Macherey-Nagel, Düren, Germany) and samples were sent to ServiceXS (Leiden, The Netherlands) for further microarray processing. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA). .. Subsequently, Illumina TotalPrep RNA Amplificationkit (Applied Biosystems/Ambion, TX) was used to create double-stranded cDNA from 500 ng total RNA.

Article Title: A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
Article Snippet: Peptides were then dried and dissolved in starting buffer (3% CH3 CN and 0.1% HCOOH, w /v ) for chromatographic elution. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The ESI LC-MS/MSdata were converted into DTA-format files that were further searched for protein identification with MASCOT Daemon (Matrix Science, [ ]) in the NCBInr-protein sequence database, Viridiplantae (green plants), and in the Brassica EST database (Brassica Genome Gateway 2007, [ ]).

Article Title: Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis
Article Snippet: Total RNA was extracted from individual livers (n = 5 per group) using RNAzol (Campro Scientific, Veenendaal, The Netherlands) and glass beads according to the manufacturer's instructions. .. The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The quality control procedure is described in Additional data file 8.

Article Title: Association of Impaired Reactive Aldehyde Metabolism with Delayed Graft Function in Human Kidney Transplantation
Article Snippet: From these renal biopsies, total RNA was extracted using RNAzol (Campro Scientific, Veenendaal, Netherlands) and glass beads. .. The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Amstelveen, Netherlands). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands and displayed no chromosomal peaks or RNA degradation products (RNA integrity number > 8.0).

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: Peptide extracts were then dried and dissolved in a starting buffer, made up of 3% CH3 CN and 0.1% HCOOH in water, for chromatographic elution. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Article Snippet: Samples were cleaned from salts that may be present using the Qiagen RNA MinElute procedure according to the manufacturer's procedure (Qiagen, Venlo, The Netherlands). .. Total RNA concentration was measured using the Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA) and RNA purity and integrity was verified using lab-on-chip technology (Agilent 2100 Bioanalyzer, Santa Clara, CA, USA). .. Genome-wide transcriptome data were collected from peripheral blood cells of 14 patients prior to the start of RTX using baseline transcript data from 13 patients generated earlier for analysis of the pharmacological changes during RTX [ ], supplemented with baseline data from an additional patient.

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: Peptide extracts were dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, MA, USA).

Article Title: Does ear C sink strength contribute to overcoming photosynthetic acclimation of wheat plants exposed to elevated CO2?
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, which consisted of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4; Bruker Daltonic, Billerica, MA, USA).

Article Title: Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress
Article Snippet: Total RNA was extracted using TRIzol® Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. Microarrays were carried out by the Shanghai Biotechnology Corporation using the One-Cycle Target Labeling kits and Affymetrix GeneChips, following manufacturers’ instructions.

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.)
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, which consisted of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the Data Analysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: Microdissection was conducted using the PixCell IIe Laser Capture Microdissection system (Molecular Devices Corporation, Sunnyvale, CA), which directs the low-power infrared laser through the cap to melt the film onto the cells of interest, which were then lifted from the tissues section RNA was extracted from whole tissue specimens as well as LCM treated samples at the Microarray Core Facility of the Texas Medical Center Digestive Disease Center at Baylor College of Medicine using the Qiagen RNeasy Extraction kit (Qiagen, Inc, Valencia, CA, USA). .. The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA). .. Gene expression analysis of whole tissue specimens was performed using the Human Affymetrix HG_U133A GeneChip® (Affymetrix Inc, Santa Clara, CA, USA) while gene expression analysis of LCM obtained specimens was performed using the Human Affymetrix HG_ Hgu133plus2 ® (Affymetrix Inc, Santa Clara, CA, USA).

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: Peptide extracts were then dried and dissolved in starting buffer for chromatographic elution, consisting of 3% CH3 CN and 0.1% HCOOH in water. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The fragmentation data were interpreted using the DataAnalysis program (version 3.4, Bruker Daltonic, Billerica, USA).

Article Title: Effect of body fat distribution on the transcription response to dietary fat interventions
Article Snippet: RNA was isolated using RNA-Bee (Campro Scientific, Veenendaal, The Netherlands) and glass beads according to the manufacturer’s instructions. .. The integrity of RNA obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The isolated RNA samples were sent to ServiceXS BV (Leiden, The Netherlands) where they were processed according to Affymetrix protocols.

Article Title: Oxidative Damage in Clinical Ischemia/Reperfusion Injury: A Reappraisal
Article Snippet: Total RNA was extracted from renal or myocardial tissues as described earlier, using GAPDH as internal control ( ). .. For expression profiling, the integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (Agilent Technologies). .. RNA preparations were considered suitable for array hybridization only if samples showed intact 18S and 28S rRNA bands, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0) ( ).

Article Title: Evidence for Proteomic and Metabolic Adaptations Associated with Alterations of Seed Yield and Quality in Sulfur-limited Brassica napu
Article Snippet: Peptide extracts were dried and dissolved in starting buffer for chromatographic elution; the buffer consisted of 3% CH3 CN and 0.1% HCOOH. .. Peptides were enriched and separated using lab-on-a-chip technology (Agilent) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The software used to generate the peak list was Data Analysis for 6300 series Ion Trap LC/MS (v3.4; Bruker Daltonique, Wissembourg, France).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: RNA was isolated by using an RNeasy midi kit (Qiagen), and 8 μg of total RNA was used for probe labeling according to the manufacturer's protocol (Affymetrix). .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix). .. Data analysis was performed by using Affymetrix GENECHIP 4.0 software.

Article Title: Upregulation of Inflammatory Genes and Downregulation of Sclerostin Gene Expression Are Key Elements in the Early Phase of Fragility Fracture Healing
Article Snippet: As this method leaves residual chemical contaminants, RNA was cleaned using a commercial kit (RNeasy mini kit, Qiagen, Germany) and genomic DNA contaminants were removed by DNaseI treatment (Qiagen, Germany). .. RNA concentration was determined spectrophotometrically (Nanodrop ND-1000 Spectrophotometer, Thermo Fisher Scientific, USA) and its integrity was assessed by lab-on-a-chip technology (Agilent RNA 6000 Nano Kit, Agilent technologies, USA) according to the manufacturer's instructions. .. RNA was stored at −80°C until further use.

Chromatography:

Article Title: Evidence for Proteomic and Metabolic Adaptations Associated with Alterations of Seed Yield and Quality in Sulfur-limited Brassica napu
Article Snippet: Paragraph title: Protein Identification via Electrospray Ionization–Liquid Chromatography-Tandem Mass Spectrometry ... Peptides were enriched and separated using lab-on-a-chip technology (Agilent) and fragmented using an on-line XCT mass spectrometer (Agilent).

Concentration Assay:

Article Title: The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Article Snippet: Samples were cleaned from salts that may be present using the Qiagen RNA MinElute procedure according to the manufacturer's procedure (Qiagen, Venlo, The Netherlands). .. Total RNA concentration was measured using the Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA) and RNA purity and integrity was verified using lab-on-chip technology (Agilent 2100 Bioanalyzer, Santa Clara, CA, USA). .. Genome-wide transcriptome data were collected from peripheral blood cells of 14 patients prior to the start of RTX using baseline transcript data from 13 patients generated earlier for analysis of the pharmacological changes during RTX [ ], supplemented with baseline data from an additional patient.

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: Microdissection was conducted using the PixCell IIe Laser Capture Microdissection system (Molecular Devices Corporation, Sunnyvale, CA), which directs the low-power infrared laser through the cap to melt the film onto the cells of interest, which were then lifted from the tissues section RNA was extracted from whole tissue specimens as well as LCM treated samples at the Microarray Core Facility of the Texas Medical Center Digestive Disease Center at Baylor College of Medicine using the Qiagen RNeasy Extraction kit (Qiagen, Inc, Valencia, CA, USA). .. The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA). .. Gene expression analysis of whole tissue specimens was performed using the Human Affymetrix HG_U133A GeneChip® (Affymetrix Inc, Santa Clara, CA, USA) while gene expression analysis of LCM obtained specimens was performed using the Human Affymetrix HG_ Hgu133plus2 ® (Affymetrix Inc, Santa Clara, CA, USA).

Article Title: Effect of body fat distribution on the transcription response to dietary fat interventions
Article Snippet: The integrity of RNA obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The isolated RNA samples were sent to ServiceXS BV (Leiden, The Netherlands) where they were processed according to Affymetrix protocols.

Article Title: Upregulation of Inflammatory Genes and Downregulation of Sclerostin Gene Expression Are Key Elements in the Early Phase of Fragility Fracture Healing
Article Snippet: As this method leaves residual chemical contaminants, RNA was cleaned using a commercial kit (RNeasy mini kit, Qiagen, Germany) and genomic DNA contaminants were removed by DNaseI treatment (Qiagen, Germany). .. RNA concentration was determined spectrophotometrically (Nanodrop ND-1000 Spectrophotometer, Thermo Fisher Scientific, USA) and its integrity was assessed by lab-on-a-chip technology (Agilent RNA 6000 Nano Kit, Agilent technologies, USA) according to the manufacturer's instructions. .. RNA was stored at −80°C until further use.

Infection:

Article Title: Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress
Article Snippet: The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA).

Tandem Mass Spectroscopy:

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: Proteins were identified by ESI-LC MS/MS (electrospray ionization-liquid chromatograpy tandem mass spectrometry). .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent).

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Does ear C sink strength contribute to overcoming photosynthetic acclimation of wheat plants exposed to elevated CO2?
Article Snippet: For protein identification by ESI-LC MS/MS, excised spots were washed several times with water and dried for a few minutes. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.)
Article Snippet: Paragraph title: Protein identification by ESI-LC MS/MS ... Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: Paragraph title: Protein identification by LC MS/MS ... Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Polymerase Chain Reaction:

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany). .. The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Article Title: Antagonistic, overlapping and distinct responses to biotic stress in rice (Oryza sativa) and interactions with abiotic stress
Article Snippet: The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. The integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA).

Marker:

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: The molecular mass (Mr ) and isoelectric point (pI) were calculated using Progenesis SameSpots software (Nonlinear Dynamics) calibrated with commercial molecular mass standards (precision protein standards pre-stained, Bio-Rad) run in a separate marker lane on the 2-D gel. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Expression of osteogenic marker genes was calculated relative to 18s rRNA levels by the comparative ΔCT method ( ). .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Fluorescence:

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Data were analyzed by using Light Cycler software version 3.5.3, using the fit point method by setting the noise band to the exponential phase of the reaction to exclude background fluorescence. .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Irradiation:

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany). .. The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Isolation:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: The purity of the isolated monocyte population was on average 90% as determined by CD14+ -staining on flow cytometry analysis. mRNA was isolated from cell collections using Trizol reagent (Invitrogen/Gibco, CA) according to the manufacturer's instruction. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Article Title: The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Article Snippet: Paragraph title: RNA isolation ... Total RNA concentration was measured using the Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA) and RNA purity and integrity was verified using lab-on-chip technology (Agilent 2100 Bioanalyzer, Santa Clara, CA, USA).

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: Paragraph title: Laser Capture Microdissection and RNA Isolation ... The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA).

Article Title: Effect of body fat distribution on the transcription response to dietary fat interventions
Article Snippet: Paragraph title: RNA isolation, labeling and hybridization ... The integrity of RNA obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: RNA was isolated by using an RNeasy midi kit (Qiagen), and 8 μg of total RNA was used for probe labeling according to the manufacturer's protocol (Affymetrix). .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Labeling:

Article Title: Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis
Article Snippet: The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands). .. The integrity of each RNA sample obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: Effect of body fat distribution on the transcription response to dietary fat interventions
Article Snippet: Paragraph title: RNA isolation, labeling and hybridization ... The integrity of RNA obtained was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a Bioanalyzer 2100 (both Agilent Technologies, Amstelveen, The Netherlands).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: RNA was isolated by using an RNeasy midi kit (Qiagen), and 8 μg of total RNA was used for probe labeling according to the manufacturer's protocol (Affymetrix). .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

Purification:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: Subsequently, mRNA was purified with NucleoSpin® RNAII (Macherey-Nagel, Düren, Germany) and samples were sent to ServiceXS (Leiden, The Netherlands) for further microarray processing. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Sequencing:

Article Title: Copper-Deficiency in Brassica napus Induces Copper Remobilization, Molybdenum Accumulation and Modification of the Expression of Chloroplastic Proteins
Article Snippet: Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent). .. Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent).

Article Title: Novel Insights into miRNA in Lung and Heart Inflammatory Diseases
Article Snippet: Among the few possibilities, 2100 Bioanalyzer, a lab on-chip technology from Agilent Technologies, offers both qualitative and quantitative estimation of miRNAs. .. For miRNA discovery, high-throughput deep sequencing (next-generation sequencing) platforms such as HiSeq 2000/Genome Analyzer IIX/Solexa (Illumina), SOLiD (ABI), GS FLX+, or 454 sequencing (Roche) are available [ ].

Article Title: Evidence for Proteomic and Metabolic Adaptations Associated with Alterations of Seed Yield and Quality in Sulfur-limited Brassica napu
Article Snippet: Peptides were enriched and separated using lab-on-a-chip technology (Agilent) and fragmented using an on-line XCT mass spectrometer (Agilent). .. The software used to generate the peak list was Data Analysis for 6300 series Ion Trap LC/MS (v3.4; Bruker Daltonique, Wissembourg, France).

Liquid Chromatography:

Article Title: Copper-Deficiency in Brassica napus Induces Copper Remobilization, Molybdenum Accumulation and Modification of the Expression of Chloroplastic Proteins
Article Snippet: Paragraph title: Protein Identification by ESI LC–MS/MS ... Peptides were enriched and separated using lab–on–a–chip technology (Agilent, Massy, France) and fragmented using an on–line XCT mass spectrometer (Agilent).

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: Paragraph title: Protein identification by LC-MS/MS ... Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Article Title: Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.)
Article Snippet: Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. Tandem mass spectrometry spectra were searched with a mass tolerance of 1.6 Da for precursor ions and 0.8 for MS/MS fragments.

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: Paragraph title: Protein identification by LC MS/MS ... Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent).

Software:

Article Title: Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction
Article Snippet: The molecular mass (Mr ) and isoelectric point (pI) were calculated using Progenesis SameSpots software (Nonlinear Dynamics) calibrated with commercial molecular mass standards (precision protein standards pre-stained, Bio-Rad) run in a separate marker lane on the 2-D gel. .. Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and analysed with an on-line XCT mass spectrometer (Agilent).

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany). .. The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: Data were analyzed by using Light Cycler software version 3.5.3, using the fit point method by setting the noise band to the exponential phase of the reaction to exclude background fluorescence. .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

SYBR Green Assay:

Article Title: cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
Article Snippet: RNA was isolated by using an RNeasy mini kit (Qiagen), and qPCR was performed by using SYBR green (Invitrogen) on a Light Cycler (Roche). .. The probe quality was verified by using lab-on-chip technology (Agilent Technologies), and samples were hybridized to Human Genome Focus arrays according to the manufacturer's protocol (Affymetrix).

RNA Extraction:

Article Title: Upregulation of Inflammatory Genes and Downregulation of Sclerostin Gene Expression Are Key Elements in the Early Phase of Fragility Fracture Healing
Article Snippet: Paragraph title: RNA extraction ... RNA concentration was determined spectrophotometrically (Nanodrop ND-1000 Spectrophotometer, Thermo Fisher Scientific, USA) and its integrity was assessed by lab-on-a-chip technology (Agilent RNA 6000 Nano Kit, Agilent technologies, USA) according to the manufacturer's instructions.

In Vitro:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA). .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Modification:

Article Title: Growth of Acinetobacter baumannii in Pellicle Enhanced the Expression of Potential Virulence Factors
Article Snippet: Peptides were enriched and separated using a lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. For protein identification, MS/MS peak lists were extracted, converted into mgf-format files and compared with the protein database using the MASCOT Daemon (version 2.1.3; Matrix Science, London, UK) search engine.

Article Title: How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)?
Article Snippet: Peptides were enriched and separated using lab-on-a-chip technology (Agilent, Massy, France) and fragmented using an on-line XCT mass spectrometer (Agilent). .. For protein identification, tandem mass spectrometry peak lists were extracted and compared with the protein database using the MASCOT Daemon (version 2.1.3; Matrix Science, London, UK) search engine.

Laser Capture Microdissection:

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: Paragraph title: Laser Capture Microdissection and RNA Isolation ... The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA).

Spectrophotometry:

Article Title: The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Article Snippet: Samples were cleaned from salts that may be present using the Qiagen RNA MinElute procedure according to the manufacturer's procedure (Qiagen, Venlo, The Netherlands). .. Total RNA concentration was measured using the Nanodrop spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA) and RNA purity and integrity was verified using lab-on-chip technology (Agilent 2100 Bioanalyzer, Santa Clara, CA, USA). .. Genome-wide transcriptome data were collected from peripheral blood cells of 14 patients prior to the start of RTX using baseline transcript data from 13 patients generated earlier for analysis of the pharmacological changes during RTX [ ], supplemented with baseline data from an additional patient.

Article Title: Upregulation of Inflammatory Genes and Downregulation of Sclerostin Gene Expression Are Key Elements in the Early Phase of Fragility Fracture Healing
Article Snippet: As this method leaves residual chemical contaminants, RNA was cleaned using a commercial kit (RNeasy mini kit, Qiagen, Germany) and genomic DNA contaminants were removed by DNaseI treatment (Qiagen, Germany). .. RNA concentration was determined spectrophotometrically (Nanodrop ND-1000 Spectrophotometer, Thermo Fisher Scientific, USA) and its integrity was assessed by lab-on-a-chip technology (Agilent RNA 6000 Nano Kit, Agilent technologies, USA) according to the manufacturer's instructions. .. RNA was stored at −80°C until further use.

Activation Assay:

Article Title: Oxidative Damage in Clinical Ischemia/Reperfusion Injury: A Reappraisal
Article Snippet: Nrf2 activation was assessed in whole-cell lysate. .. For expression profiling, the integrity of each RNA sample was examined by Agilent Lab-on-a-chip technology using the RNA 6000 Nano LabChip kit and a bioanalyzer 2100 (Agilent Technologies).

CTG Assay:

Article Title: Interferon-?4 (IFNL4) Transcript Expression in Human Liver Tissue Samples
Article Snippet: Forward and reverse primers were chosen complementary to sequences within exon 3 ( 5′-GAG GGA TGT GGC GGC CTG-3′ ) and exon 5 ( 5′-GAC CAC GCT GGC TTT GCG-3′ ), respectively, and a FAM-labeled minor groove binder (MGB) probe (5′-CCC GGA GAG CGG AC-3′) spanning the exon 4–5 boundary to further enhance signal specificity. .. The expected size of the amplicon of 129 bp was confirmed by electrophoretic separation using lab-on-a-chip technology in an Agilent 2100 bioanalyzer (DNA 1000 LabChip kit, Agilent Technologies, Boeblingen, Germany).

Staining:

Article Title: Short-Term Erythropoietin Treatment Does Not Substantially Modulate Monocyte Transcriptomes of Patients with Combined Heart and Renal Failure
Article Snippet: The purity of the isolated monocyte population was on average 90% as determined by CD14+ -staining on flow cytometry analysis. mRNA was isolated from cell collections using Trizol reagent (Invitrogen/Gibco, CA) according to the manufacturer's instruction. .. In brief, quality and integrity of RNA was checked by lab-on-chip technology (Bioanalyzer Agilent, CA).

Article Title: Gene Expression in Barrett's Esophagus: Laser Capture vs. Whole tissue
Article Snippet: The frozen tissue samples were cut with the Cryotome (Thermo Fisher Scientific, Waltham, MA), stained with Histogene Staining Solution (Molecular Devices Corporation, Sunnyvale, CA) in RNA-free surroundings. .. The RNA integrity, quality, and concentration were measured using the Agilent 2100 Bioanalyzer and Lab-on-a-Chip technology (Agilent Technologies, Palo Alto, CA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Agilent technologies lab on a chip system
    <t>UV-LMD</t> of individual neurons. ( A ) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV-laser-microdissection of the entire area. Right panel: gel electrophoresis results of qualitative reverse transcription (RT) multiplex-nested PCR products for a whole LMD SNpc. RT–PCR signals for all eight different dopaminergic (TH, DAT, Girk2, D2s/l) and nondopaminergic (GAD, Girk1, GFAP, CB) marker-genes, detected in the heterogeneous cellular mixture of dopaminergic, GABAergic and glial cells. (DNA-ladder: 100-bp marker). ( B ) Top: CV-stained coronal midbrain section after UV-laser-microdissection of 15 individual DA neurons. Middle: selection and laser-microdissection of an individual neuron from upper section. Lower: after UV-LMD of individual neuron—section (left) and cap-control (right) demonstrating successful isolation. ( C ) PCR products after gel electrophoresis of qualitative RT multiplex-nested PCR for individual LMD SN DA neurons and small SN DA pools. Upper and second panel: similar gene-expression profile of dopaminergic marker genes (TH, DAT, Girk2, D2; not Girk2, GAD67, GFAP) in pools of 20 and single SN DA neurons illustrate sensitivity and specificity of the protocol. Third panel: expression profile of an individual calbindin-positive CB (+) SN DA neuron ( D and E ) Quantitative real-time RT–PCR analysis of Eno2 gene-expression using 10% of cDNA from pools of 15 mouse SN DA neurons as template. Neurons were UV-LMD-collected from either fresh or stored (1-week) and re-used tissue slices. (D) Representative real-time PCR traces testing for Eno2 expression, relative fluorescence levels on a logarithmic scale are plotted against PCR cycles (ΔRN: relative fluorescence, normalized to internal fluorescence marker ROX); threshold-line for determinations of threshold cycles (Ct) indicated by the green line (Δ RN 0.4). (E) No significant difference n.s. between Eno2 detection levels (Ct) in SN DA cell-pools from fresh and frozen tissue sections (fresh: 34.43 ± 0.51, n = 10; re-used: 34.62 ± 0.48 n = 9; p = 0.785).
    Lab On A Chip System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lab on a chip system/product/Agilent technologies
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lab on a chip system - by Bioz Stars, 2019-10
    91/100 stars
      Buy from Supplier

    99
    Agilent technologies high sensitivity dna assay kit
    Qualitive <t>cf-DNA</t> analysis. Qualitative analysis of plasma cf-DNA in 8 bacteremia survivors (panel A) and 8 non-survivors (panel B) after NucleoSpin® Plasma XS kit extraction. Analyses were performed with <t>Agilent's</t> High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low-weight (35 bp) DNA marker and purple lines the high- weight (10 380 bp) DNA marker. TP 1 = time point 1 (1 to 4 days after blood culture), TP 2 = time point 2 (5 to 17 days after blood culture), TP 3 = time point 3 (recovery, > 25 days after blood culture). The intensity of the low-molecular-weight cf-DNA band was graded as follows: 1 = no visible cf-DNA or weak intensity, 2 = intermediate intensity, 3 = strong intensity. Abbreviations; bp = base pairs, TP = time point.
    High Sensitivity Dna Assay Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna assay kit/product/Agilent technologies
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna assay kit - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies small rna kit
    Specificity of the locked nucleic acid (LNA) probes for the detection of miR and pre-miR using in situ hybridization. Using 2.5 pmol of locked nucleic acid (LNA) probes prelabelled with digoxigenin, we determined in situ hybridization patterns of scramble miR (A; negative control), U6 small nuclear <t>RNA</t> (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 1). In the second lane, Digoxigenin-labeled probes were competed with excessive amount (25 pmol ie 10× more) of unlabeled probes. Most of the signal observed in the panels of the first lane are absent from the panels in the second lane. Scale bars = 10 µm. Images are not scaled to the same intensity range. Positive in situ hybridization signals showed were normalized by scramble <t>mirna</t> signal intensity (negative control).
    Small Rna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/small rna kit/product/Agilent technologies
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    small rna kit - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    UV-LMD of individual neurons. ( A ) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV-laser-microdissection of the entire area. Right panel: gel electrophoresis results of qualitative reverse transcription (RT) multiplex-nested PCR products for a whole LMD SNpc. RT–PCR signals for all eight different dopaminergic (TH, DAT, Girk2, D2s/l) and nondopaminergic (GAD, Girk1, GFAP, CB) marker-genes, detected in the heterogeneous cellular mixture of dopaminergic, GABAergic and glial cells. (DNA-ladder: 100-bp marker). ( B ) Top: CV-stained coronal midbrain section after UV-laser-microdissection of 15 individual DA neurons. Middle: selection and laser-microdissection of an individual neuron from upper section. Lower: after UV-LMD of individual neuron—section (left) and cap-control (right) demonstrating successful isolation. ( C ) PCR products after gel electrophoresis of qualitative RT multiplex-nested PCR for individual LMD SN DA neurons and small SN DA pools. Upper and second panel: similar gene-expression profile of dopaminergic marker genes (TH, DAT, Girk2, D2; not Girk2, GAD67, GFAP) in pools of 20 and single SN DA neurons illustrate sensitivity and specificity of the protocol. Third panel: expression profile of an individual calbindin-positive CB (+) SN DA neuron ( D and E ) Quantitative real-time RT–PCR analysis of Eno2 gene-expression using 10% of cDNA from pools of 15 mouse SN DA neurons as template. Neurons were UV-LMD-collected from either fresh or stored (1-week) and re-used tissue slices. (D) Representative real-time PCR traces testing for Eno2 expression, relative fluorescence levels on a logarithmic scale are plotted against PCR cycles (ΔRN: relative fluorescence, normalized to internal fluorescence marker ROX); threshold-line for determinations of threshold cycles (Ct) indicated by the green line (Δ RN 0.4). (E) No significant difference n.s. between Eno2 detection levels (Ct) in SN DA cell-pools from fresh and frozen tissue sections (fresh: 34.43 ± 0.51, n = 10; re-used: 34.62 ± 0.48 n = 9; p = 0.785).

    Journal: Nucleic Acids Research

    Article Title: Elevated ?-synuclein mRNA levels in individual UV-laser-microdissected dopaminergic substantia nigra neurons in idiopathic Parkinson's disease

    doi: 10.1093/nar/gkn084

    Figure Lengend Snippet: UV-LMD of individual neurons. ( A ) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV-laser-microdissection of the entire area. Right panel: gel electrophoresis results of qualitative reverse transcription (RT) multiplex-nested PCR products for a whole LMD SNpc. RT–PCR signals for all eight different dopaminergic (TH, DAT, Girk2, D2s/l) and nondopaminergic (GAD, Girk1, GFAP, CB) marker-genes, detected in the heterogeneous cellular mixture of dopaminergic, GABAergic and glial cells. (DNA-ladder: 100-bp marker). ( B ) Top: CV-stained coronal midbrain section after UV-laser-microdissection of 15 individual DA neurons. Middle: selection and laser-microdissection of an individual neuron from upper section. Lower: after UV-LMD of individual neuron—section (left) and cap-control (right) demonstrating successful isolation. ( C ) PCR products after gel electrophoresis of qualitative RT multiplex-nested PCR for individual LMD SN DA neurons and small SN DA pools. Upper and second panel: similar gene-expression profile of dopaminergic marker genes (TH, DAT, Girk2, D2; not Girk2, GAD67, GFAP) in pools of 20 and single SN DA neurons illustrate sensitivity and specificity of the protocol. Third panel: expression profile of an individual calbindin-positive CB (+) SN DA neuron ( D and E ) Quantitative real-time RT–PCR analysis of Eno2 gene-expression using 10% of cDNA from pools of 15 mouse SN DA neurons as template. Neurons were UV-LMD-collected from either fresh or stored (1-week) and re-used tissue slices. (D) Representative real-time PCR traces testing for Eno2 expression, relative fluorescence levels on a logarithmic scale are plotted against PCR cycles (ΔRN: relative fluorescence, normalized to internal fluorescence marker ROX); threshold-line for determinations of threshold cycles (Ct) indicated by the green line (Δ RN 0.4). (E) No significant difference n.s. between Eno2 detection levels (Ct) in SN DA cell-pools from fresh and frozen tissue sections (fresh: 34.43 ± 0.51, n = 10; re-used: 34.62 ± 0.48 n = 9; p = 0.785).

    Article Snippet: We analyzed RNA quality of all human midbrain tissues of this study from spare, unfixed, unstained sections and chippings collected during cryosectioning and before mounting of tissue for LMD using the Agilent Lab-on-a-Chip System, which calculates the RIN.

    Techniques: Laser Capture Microdissection, Staining, Nucleic Acid Electrophoresis, Multiplex Assay, Nested PCR, Reverse Transcription Polymerase Chain Reaction, Direct Antiglobulin Test, Marker, Selection, Isolation, Polymerase Chain Reaction, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Fluorescence

    Qualitive cf-DNA analysis. Qualitative analysis of plasma cf-DNA in 8 bacteremia survivors (panel A) and 8 non-survivors (panel B) after NucleoSpin® Plasma XS kit extraction. Analyses were performed with Agilent's High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low-weight (35 bp) DNA marker and purple lines the high- weight (10 380 bp) DNA marker. TP 1 = time point 1 (1 to 4 days after blood culture), TP 2 = time point 2 (5 to 17 days after blood culture), TP 3 = time point 3 (recovery, > 25 days after blood culture). The intensity of the low-molecular-weight cf-DNA band was graded as follows: 1 = no visible cf-DNA or weak intensity, 2 = intermediate intensity, 3 = strong intensity. Abbreviations; bp = base pairs, TP = time point.

    Journal: PLoS ONE

    Article Title: Fatal Outcome in Bacteremia is Characterized by High Plasma Cell Free DNA Concentration and Apoptotic DNA Fragmentation: A Prospective Cohort Study

    doi: 10.1371/journal.pone.0021700

    Figure Lengend Snippet: Qualitive cf-DNA analysis. Qualitative analysis of plasma cf-DNA in 8 bacteremia survivors (panel A) and 8 non-survivors (panel B) after NucleoSpin® Plasma XS kit extraction. Analyses were performed with Agilent's High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low-weight (35 bp) DNA marker and purple lines the high- weight (10 380 bp) DNA marker. TP 1 = time point 1 (1 to 4 days after blood culture), TP 2 = time point 2 (5 to 17 days after blood culture), TP 3 = time point 3 (recovery, > 25 days after blood culture). The intensity of the low-molecular-weight cf-DNA band was graded as follows: 1 = no visible cf-DNA or weak intensity, 2 = intermediate intensity, 3 = strong intensity. Abbreviations; bp = base pairs, TP = time point.

    Article Snippet: Extracted cf-DNA samples were analyzed with the High Sensitivity DNA assay kit and an Agilent 2100 Bioanalyzer equipped with Expert 2100 software according to the manufacturer's instructions (Agilent Technologies Inc., Santa Clara, CA).

    Techniques: Lab-on-a-Chip, Marker, Molecular Weight

    Qualitative analysis of urine cf-DNA after NucleoSpin® Plasma XS kit extraction. Analyses were performed with Agilent's High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low weight (35 base pairs (bp)) DNA marker and purple lines the high weight (10 380 bp) DNA marker. During the acute phase of the disease low-molecular weight (150–200 bp) pattern of cf-DNA was detected only in patients no 5 and 7, while patients no 3 and 20 had random-sized cf-DNA fragments in their control urine samples. Data from patients no 11 and 14 are depicted as examples of the 16 subjects who had no findings in the urinary cf-DNA fragment analyses.

    Journal: PLoS ONE

    Article Title: Plasma Cell-Free DNA Levels Are Elevated in Acute Puumala Hantavirus Infection

    doi: 10.1371/journal.pone.0031455

    Figure Lengend Snippet: Qualitative analysis of urine cf-DNA after NucleoSpin® Plasma XS kit extraction. Analyses were performed with Agilent's High Sensitivity Lab-on-a-chip DNA assay. Green lines indicate the low weight (35 base pairs (bp)) DNA marker and purple lines the high weight (10 380 bp) DNA marker. During the acute phase of the disease low-molecular weight (150–200 bp) pattern of cf-DNA was detected only in patients no 5 and 7, while patients no 3 and 20 had random-sized cf-DNA fragments in their control urine samples. Data from patients no 11 and 14 are depicted as examples of the 16 subjects who had no findings in the urinary cf-DNA fragment analyses.

    Article Snippet: Extracted cf-DNA samples were analyzed with the High Sensitivity DNA assay kit and an Agilent 2100 Bioanalyzer equipped with Expert 2100 software according to the manufacturer's instructions (Agilent Technologies Inc., Santa Clara, CA).

    Techniques: Lab-on-a-Chip, Marker, Molecular Weight

    Specificity of the locked nucleic acid (LNA) probes for the detection of miR and pre-miR using in situ hybridization. Using 2.5 pmol of locked nucleic acid (LNA) probes prelabelled with digoxigenin, we determined in situ hybridization patterns of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 1). In the second lane, Digoxigenin-labeled probes were competed with excessive amount (25 pmol ie 10× more) of unlabeled probes. Most of the signal observed in the panels of the first lane are absent from the panels in the second lane. Scale bars = 10 µm. Images are not scaled to the same intensity range. Positive in situ hybridization signals showed were normalized by scramble mirna signal intensity (negative control).

    Journal: PLoS ONE

    Article Title: Pre-microRNA and Mature microRNA in Human Mitochondria

    doi: 10.1371/journal.pone.0020220

    Figure Lengend Snippet: Specificity of the locked nucleic acid (LNA) probes for the detection of miR and pre-miR using in situ hybridization. Using 2.5 pmol of locked nucleic acid (LNA) probes prelabelled with digoxigenin, we determined in situ hybridization patterns of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 1). In the second lane, Digoxigenin-labeled probes were competed with excessive amount (25 pmol ie 10× more) of unlabeled probes. Most of the signal observed in the panels of the first lane are absent from the panels in the second lane. Scale bars = 10 µm. Images are not scaled to the same intensity range. Positive in situ hybridization signals showed were normalized by scramble mirna signal intensity (negative control).

    Article Snippet: It was a microfluidic lab-on-a-chip technology using electrophoresis and dye to separate and detect RNA of 0 to 150 nt and the percentage of miRNA which was shorter than 40 nt (Small RNA kit, 2100 Bioanalyzer®, Agilent Technologies) .

    Techniques: In Situ Hybridization, Negative Control, Positive Control, Labeling

    In situ hybridization pattern of digoxigenin-labeled Locked Nucleic Acid (LNA) for specific miR and pre-miR in human skeletal muscle myoblasts cells observed in classic optic microscopy. Using locked nucleic acid (LNA) probes digoxigenin labelled, we determined the in situ hybridization pattern of mir and pre-mir. Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miRNA (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 2) and MitoTracker® Red CM-H 2 XRos staining of respiring mitochondria (lane 3) are represented in gray scale. All these images were acquired using an Olympus BX61 straight microscope controlled with Metamorph software (Molecular Devices, Downington, PA19335) using a 100× oil-immersion objective. In the overlays (lane 4) provided by Image J software, positive in situ hybridization signals are visualized in green, respiring mitochondria signal in red and nuclei staining in blue. Yellow staining suggests co-localization of LNA probes (green fluorescence) and MitoTracker® Red CM-H 2 Ros (red fluorescence). Scale bars = 10 µm. Images are not scaled to the same intensity range. Positive in situ hybridization signals were normalized by scramble miR signal intensity (negative control).

    Journal: PLoS ONE

    Article Title: Pre-microRNA and Mature microRNA in Human Mitochondria

    doi: 10.1371/journal.pone.0020220

    Figure Lengend Snippet: In situ hybridization pattern of digoxigenin-labeled Locked Nucleic Acid (LNA) for specific miR and pre-miR in human skeletal muscle myoblasts cells observed in classic optic microscopy. Using locked nucleic acid (LNA) probes digoxigenin labelled, we determined the in situ hybridization pattern of mir and pre-mir. Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miRNA (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) probes (lane 2) and MitoTracker® Red CM-H 2 XRos staining of respiring mitochondria (lane 3) are represented in gray scale. All these images were acquired using an Olympus BX61 straight microscope controlled with Metamorph software (Molecular Devices, Downington, PA19335) using a 100× oil-immersion objective. In the overlays (lane 4) provided by Image J software, positive in situ hybridization signals are visualized in green, respiring mitochondria signal in red and nuclei staining in blue. Yellow staining suggests co-localization of LNA probes (green fluorescence) and MitoTracker® Red CM-H 2 Ros (red fluorescence). Scale bars = 10 µm. Images are not scaled to the same intensity range. Positive in situ hybridization signals were normalized by scramble miR signal intensity (negative control).

    Article Snippet: It was a microfluidic lab-on-a-chip technology using electrophoresis and dye to separate and detect RNA of 0 to 150 nt and the percentage of miRNA which was shorter than 40 nt (Small RNA kit, 2100 Bioanalyzer®, Agilent Technologies) .

    Techniques: In Situ Hybridization, Labeling, Microscopy, Staining, Negative Control, Positive Control, Software, Fluorescence

    In situ signal of mir-365, pre-mir-let7b and pre-mir-302a co-localized with functioning mitochondria in human myoblasts observed in confocal microscopy. Using locked nucleic acid (LNA) probes, we performed in situ hybridization to localized mir-365, mir-let-7b, pre-let-7b and pre-mir-302a within the cell. Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) provided by locked nucleic acid (LNA) probes (lane 2) and MitoTracker® Red CM-H 2 XRos staining of functioning mitochondria (lane 3) are represented in gray scale. All these images were acquired with a Leica TSC-P2 confocal microscope using a 63× oil-immersion objective. A sequential mode for three colour of acquisition (FITC for LNA probes signals, Dapi for Hoechst staining and Cy3 for MitoTracker® Red CM-H 2 XRos) has been used. In the overlays (lane 4) provided by Image J software, positive in situ hybridization signal are visualized in green and respiring mitochondria signal in red. Yellow corresponded to red and green overlay. The pixels with co-localized signals (lane 5) from functioning mitochondria and specific LNA probe for mir or pre-mir are determined using the Image J MBF plugging “co-localization highlighter”. Scale bars = 10 µm. The raw images are showed in the figure. The percentage of pixels with co-localized miRNA and mitochondrial signals were determined using Isodata plugging threshold (Image J software) and indicated on the figure (% in lane 5).

    Journal: PLoS ONE

    Article Title: Pre-microRNA and Mature microRNA in Human Mitochondria

    doi: 10.1371/journal.pone.0020220

    Figure Lengend Snippet: In situ signal of mir-365, pre-mir-let7b and pre-mir-302a co-localized with functioning mitochondria in human myoblasts observed in confocal microscopy. Using locked nucleic acid (LNA) probes, we performed in situ hybridization to localized mir-365, mir-let-7b, pre-let-7b and pre-mir-302a within the cell. Hoechst 33342 staining of nuclei (lane 1), specific signal of scramble miR (A; negative control), U6 small nuclear RNA (B; positive control), let-7b (C), mir-365 (D), pre-let-7b (E) and pre-mir-302a (F) provided by locked nucleic acid (LNA) probes (lane 2) and MitoTracker® Red CM-H 2 XRos staining of functioning mitochondria (lane 3) are represented in gray scale. All these images were acquired with a Leica TSC-P2 confocal microscope using a 63× oil-immersion objective. A sequential mode for three colour of acquisition (FITC for LNA probes signals, Dapi for Hoechst staining and Cy3 for MitoTracker® Red CM-H 2 XRos) has been used. In the overlays (lane 4) provided by Image J software, positive in situ hybridization signal are visualized in green and respiring mitochondria signal in red. Yellow corresponded to red and green overlay. The pixels with co-localized signals (lane 5) from functioning mitochondria and specific LNA probe for mir or pre-mir are determined using the Image J MBF plugging “co-localization highlighter”. Scale bars = 10 µm. The raw images are showed in the figure. The percentage of pixels with co-localized miRNA and mitochondrial signals were determined using Isodata plugging threshold (Image J software) and indicated on the figure (% in lane 5).

    Article Snippet: It was a microfluidic lab-on-a-chip technology using electrophoresis and dye to separate and detect RNA of 0 to 150 nt and the percentage of miRNA which was shorter than 40 nt (Small RNA kit, 2100 Bioanalyzer®, Agilent Technologies) .

    Techniques: In Situ, Confocal Microscopy, In Situ Hybridization, Staining, Negative Control, Positive Control, Microscopy, Software