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Agilent technologies lab on a chip device
Lab On A Chip Device, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lab on a chip device/product/Agilent technologies
Average 88 stars, based on 5 article reviews
Price from $9.99 to $1999.99
lab on a chip device - by Bioz Stars, 2020-05
88/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Genome-wide transcriptome analysis of 150 cell samples †
Article Snippet: .. To estimate the quality of the total RNA isolated from the THP1 cell line using the microfluidic device, we performed gel electrophoresis in the Agilent Bioanalyzer Pico Chip. ..

Lab-on-a-Chip:

Article Title: Microfluidic opportunities in the field of nutrition
Article Snippet: .. To compare the performance of this lab-on-a-chip device with conventional CE, Blazek et al . analyzed the protein content of twenty different soybean cultivars with both conventional CE and the Agilent 2100 Bioanalyzer. .. In addition to increased time-efficiency, the lab-on-a-chip device demonstrated better performance in terms of the repeatability of migration times and peak areas.

Article Title: Synthetic Scrapie Infectivity: Interaction between Recombinant PrP and Scrapie Brain-Derived RNA
Article Snippet: .. Aliquots of the test solutions (1 μl each) from the PrPSc /infectivity elimination procedures (or from the inocula containing recPrP) were added to wells of a lab-on-a-chip device (2100 Bioanalyzer, Small RNA Assay and Large RNA integrity assay, Agilent Technologies) and analyzed according to the manufacturer's recommendations. .. Samples of the inocula (5 μl each) prepared as described above were deposited onto grids and negatively stained as previously described.

Article Title: S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer
Article Snippet: .. A lab-on-a-chip device, RNA labchio, read on a Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA) was used to evaluate the RNA quantity. .. The GMRCL Human 7K set, version 1 chips (InCyte Genomics, Palo Alto, CA, USA) containing 7,334 sequence-verified human genes were used in the present study.

Fluorescence:

Article Title: Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding
Article Snippet: .. Different aliquots of HeLa cells were run through the microfluidic device and counted independently by a commercial fluorescence-activated cell sorter (MoFlo FACS system, DakoCytomation) to test the accuracy of our microfluidic cell counter. .. FACS detection parameters were preset for our HeLa cells by calibration with a test sample of HeLa cells.

Isolation:

Article Title: Genome-wide transcriptome analysis of 150 cell samples †
Article Snippet: .. To estimate the quality of the total RNA isolated from the THP1 cell line using the microfluidic device, we performed gel electrophoresis in the Agilent Bioanalyzer Pico Chip. ..

Infection:

Article Title: Synthetic Scrapie Infectivity: Interaction between Recombinant PrP and Scrapie Brain-Derived RNA
Article Snippet: .. Aliquots of the test solutions (1 μl each) from the PrPSc /infectivity elimination procedures (or from the inocula containing recPrP) were added to wells of a lab-on-a-chip device (2100 Bioanalyzer, Small RNA Assay and Large RNA integrity assay, Agilent Technologies) and analyzed according to the manufacturer's recommendations. .. Samples of the inocula (5 μl each) prepared as described above were deposited onto grids and negatively stained as previously described.

Integrity Assay:

Article Title: Synthetic Scrapie Infectivity: Interaction between Recombinant PrP and Scrapie Brain-Derived RNA
Article Snippet: .. Aliquots of the test solutions (1 μl each) from the PrPSc /infectivity elimination procedures (or from the inocula containing recPrP) were added to wells of a lab-on-a-chip device (2100 Bioanalyzer, Small RNA Assay and Large RNA integrity assay, Agilent Technologies) and analyzed according to the manufacturer's recommendations. .. Samples of the inocula (5 μl each) prepared as described above were deposited onto grids and negatively stained as previously described.

FACS:

Article Title: Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding
Article Snippet: .. Different aliquots of HeLa cells were run through the microfluidic device and counted independently by a commercial fluorescence-activated cell sorter (MoFlo FACS system, DakoCytomation) to test the accuracy of our microfluidic cell counter. .. FACS detection parameters were preset for our HeLa cells by calibration with a test sample of HeLa cells.

Chromatin Immunoprecipitation:

Article Title: Genome-wide transcriptome analysis of 150 cell samples †
Article Snippet: .. To estimate the quality of the total RNA isolated from the THP1 cell line using the microfluidic device, we performed gel electrophoresis in the Agilent Bioanalyzer Pico Chip. ..

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  • 93
    Agilent technologies dna microarray analysis
    Comparison of the gene expressions of SYM-I cells infected with Ni, Ni-CE, and CE(NiN) strains using a <t>DNA</t> <t>microarray.</t> SYM-I cells were infected with Ni, Ni-CE, and CE(NiN) strains at an MOI of 2. After 24 h, the total cellular RNA was extracted and used for DNA microarray analysis. The data were normalized by GeneSpring GX software. (A) Cluster analysis of genes of SYM-I cells infected with each virus. The expression pattern of genes involved in “host-pathogen interaction” is represented as a hierarchical clustering, using Cluster and Java TreeView. Genes shown in red are upregulated, and those shown in green are downregulated relative to mock-infected cells. (B) Expression levels of 10 host immunity-related genes, most of which were differentially expressed in Ni-CE- and CE(NiN)-infected cells. Each bar represents the fold change in expression compared to the expression level of each gene in mock-infected cells.
    Dna Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray analysis/product/Agilent technologies
    Average 93 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    dna microarray analysis - by Bioz Stars, 2020-05
    93/100 stars
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    92
    Agilent technologies microfluidic device
    <t>Microfluidic</t> device for the isolation of RNA from small numbers of cells. A: Overall protocol for high quality RNA isolation. Mammalian cells are captured and lysed on the chip, in the presence of a high concentration of chaotropic agent. Cellular proteins
    Microfluidic Device, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfluidic device/product/Agilent technologies
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microfluidic device - by Bioz Stars, 2020-05
    92/100 stars
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    99
    Agilent technologies microarray analysis
    Effect of low concentrations of Na 2 SeO 3 treatment on E. coli cell growth and selenite reduction. (a) The effect of 0 to 10 mM Na 2 SeO 3 on cell growth rates. The experiment was performed with three biological replicates and each Na 2 SeO 3 treated sample was analyzed in triplicate. Data plotted was the average of growth ± SD. The concentrations (0, 0.01, and 5 mM) that were marked in blue were used to prepare RNA samples for <t>microarray</t> analysis. (b) Color change in cultures after treatments with 0 to 10 mM Na 2 SeO 3 . * P ≤ 0.05.
    Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray analysis/product/Agilent technologies
    Average 99 stars, based on 1221 article reviews
    Price from $9.99 to $1999.99
    microarray analysis - by Bioz Stars, 2020-05
    99/100 stars
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    Comparison of the gene expressions of SYM-I cells infected with Ni, Ni-CE, and CE(NiN) strains using a DNA microarray. SYM-I cells were infected with Ni, Ni-CE, and CE(NiN) strains at an MOI of 2. After 24 h, the total cellular RNA was extracted and used for DNA microarray analysis. The data were normalized by GeneSpring GX software. (A) Cluster analysis of genes of SYM-I cells infected with each virus. The expression pattern of genes involved in “host-pathogen interaction” is represented as a hierarchical clustering, using Cluster and Java TreeView. Genes shown in red are upregulated, and those shown in green are downregulated relative to mock-infected cells. (B) Expression levels of 10 host immunity-related genes, most of which were differentially expressed in Ni-CE- and CE(NiN)-infected cells. Each bar represents the fold change in expression compared to the expression level of each gene in mock-infected cells.

    Journal: Journal of Virology

    Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿

    doi: 10.1128/JVI.02220-09

    Figure Lengend Snippet: Comparison of the gene expressions of SYM-I cells infected with Ni, Ni-CE, and CE(NiN) strains using a DNA microarray. SYM-I cells were infected with Ni, Ni-CE, and CE(NiN) strains at an MOI of 2. After 24 h, the total cellular RNA was extracted and used for DNA microarray analysis. The data were normalized by GeneSpring GX software. (A) Cluster analysis of genes of SYM-I cells infected with each virus. The expression pattern of genes involved in “host-pathogen interaction” is represented as a hierarchical clustering, using Cluster and Java TreeView. Genes shown in red are upregulated, and those shown in green are downregulated relative to mock-infected cells. (B) Expression levels of 10 host immunity-related genes, most of which were differentially expressed in Ni-CE- and CE(NiN)-infected cells. Each bar represents the fold change in expression compared to the expression level of each gene in mock-infected cells.

    Article Snippet: RNA preparations used for DNA microarray analysis were analyzed with a lab-on-a-chip Agilent Bioanalyzer (RNA 6000 LabChip kit; Agilent) to confirm the concentration, integrity, and purity.

    Techniques: Infection, Microarray, Software, Expressing

    Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Journal: Journal of Virology

    Article Title: Rabies Virus Nucleoprotein Functions To Evade Activation of the RIG-I-Mediated Antiviral Response ▿

    doi: 10.1128/JVI.02220-09

    Figure Lengend Snippet: Validation by real-time RT-PCR of DNA microarray results for IFN -β (A), IFN -λ 1 (B), CXCL10 (C), and CCL5 (D). The assay was performed with the same total RNA used in the DNA microarray experiment. Expression levels of genes were normalized to mRNA levels of GAPDH . Each bar represents the mean (± the SD) of three independent replicates. *, Significant difference ( P

    Article Snippet: RNA preparations used for DNA microarray analysis were analyzed with a lab-on-a-chip Agilent Bioanalyzer (RNA 6000 LabChip kit; Agilent) to confirm the concentration, integrity, and purity.

    Techniques: Quantitative RT-PCR, Microarray, Expressing

    Microfluidic device for the isolation of RNA from small numbers of cells. A: Overall protocol for high quality RNA isolation. Mammalian cells are captured and lysed on the chip, in the presence of a high concentration of chaotropic agent. Cellular proteins

    Journal:

    Article Title: Genome-wide transcriptome analysis of 150 cell samples †

    doi: 10.1039/b814329c

    Figure Lengend Snippet: Microfluidic device for the isolation of RNA from small numbers of cells. A: Overall protocol for high quality RNA isolation. Mammalian cells are captured and lysed on the chip, in the presence of a high concentration of chaotropic agent. Cellular proteins

    Article Snippet: To estimate the quality of the total RNA isolated from the THP1 cell line using the microfluidic device, we performed gel electrophoresis in the Agilent Bioanalyzer Pico Chip.

    Techniques: Isolation, Chromatin Immunoprecipitation, Concentration Assay

    Quality of RNA isolated using the microfluidic devices. A: Removal of genomic DNA from the isolated RNA. RNA samples with and without on-chip 5 min DNase treatment, isolated from 50 cell samples using the microfluidic device, are split into two and GAPDH

    Journal:

    Article Title: Genome-wide transcriptome analysis of 150 cell samples †

    doi: 10.1039/b814329c

    Figure Lengend Snippet: Quality of RNA isolated using the microfluidic devices. A: Removal of genomic DNA from the isolated RNA. RNA samples with and without on-chip 5 min DNase treatment, isolated from 50 cell samples using the microfluidic device, are split into two and GAPDH

    Article Snippet: To estimate the quality of the total RNA isolated from the THP1 cell line using the microfluidic device, we performed gel electrophoresis in the Agilent Bioanalyzer Pico Chip.

    Techniques: Isolation, Chromatin Immunoprecipitation

    a) Assembled microfluidic chip, b) a microscopy picture of gold electrode and micro-wells, c) SEM image of one micro-well, d) the working principle of microfluidic device, and e) the computational domain consisting of medium solution, SU-8, and spherical yeast cells. The yeast cells are modeled consisting of an inner cytoplasm covered by a membrane modelled as an interface.

    Journal: Electrophoresis

    Article Title: DIELECTROPHORESIS ASSISTED LOADING AND UNLOADING OF MICRO-WELLS FOR IMPEDANCE SPECTROSCOPY

    doi: 10.1002/elps.201700020

    Figure Lengend Snippet: a) Assembled microfluidic chip, b) a microscopy picture of gold electrode and micro-wells, c) SEM image of one micro-well, d) the working principle of microfluidic device, and e) the computational domain consisting of medium solution, SU-8, and spherical yeast cells. The yeast cells are modeled consisting of an inner cytoplasm covered by a membrane modelled as an interface.

    Article Snippet: Firstly, biological cells are captured inside the micro-wells using pDEP forces and then microfluidic device is connected to a high precision impedance analyzer (HP Agilent 4194A) and the impedance spectra are measured.

    Techniques: Chromatin Immunoprecipitation, Microscopy

    Effect of low concentrations of Na 2 SeO 3 treatment on E. coli cell growth and selenite reduction. (a) The effect of 0 to 10 mM Na 2 SeO 3 on cell growth rates. The experiment was performed with three biological replicates and each Na 2 SeO 3 treated sample was analyzed in triplicate. Data plotted was the average of growth ± SD. The concentrations (0, 0.01, and 5 mM) that were marked in blue were used to prepare RNA samples for microarray analysis. (b) Color change in cultures after treatments with 0 to 10 mM Na 2 SeO 3 . * P ≤ 0.05.

    Journal: International Journal of Microbiology

    Article Title: Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction

    doi: 10.1155/2014/394835

    Figure Lengend Snippet: Effect of low concentrations of Na 2 SeO 3 treatment on E. coli cell growth and selenite reduction. (a) The effect of 0 to 10 mM Na 2 SeO 3 on cell growth rates. The experiment was performed with three biological replicates and each Na 2 SeO 3 treated sample was analyzed in triplicate. Data plotted was the average of growth ± SD. The concentrations (0, 0.01, and 5 mM) that were marked in blue were used to prepare RNA samples for microarray analysis. (b) Color change in cultures after treatments with 0 to 10 mM Na 2 SeO 3 . * P ≤ 0.05.

    Article Snippet: For microarray analysis, RNA quality was further checked using a dual beam spectrophotometer and an Agilent Bioanalyzer 2100 Lab-on-a-Chip system.

    Techniques: Microarray