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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab <t>(ECL)</t> platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.
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Image Search Results


a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab (ECL) platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.

Journal: Nature Communications

Article Title: High-throughput DNA melt measurements enable improved models of DNA folding thermodynamics

doi: 10.1038/s41467-025-60455-4

Figure Lengend Snippet: a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab (ECL) platform. Magenta arrows indicate semi-automatic steps, and teal arrows indicate automatic steps. b Example UV melting curve of the variant WC2318. Pink dots represent measured data points of the cooling curve; the green solid line represents the fitted curve; the green dotted line represents the fitted melting temperature. c Mean absolute error (MAE) of ΔG, ΔH, and T m between Array Melt data and UV melting data before and after systematic error correction. Gray bars represent errors before systematic correction; colored bars after applying correction with a single offset parameter. d Direct comparison of measured ΔG 37 , ΔH, and T m between Array Melt and UV melting data. Each datapoint represents one hairpin variant. Error bars indicate Array Melt bootstrapped standard errors (y-axis). Gray dots are before systematic correction; colored dots are after. e Pearson’s correlation coefficient ( R ) between UV melting data and either Array Melt data or the dna04 model predictions. The colored bars represent the correlation between Array Melt and UV melting data; the black bars represent that between the dna04 model predictions and UV melting data.

Article Snippet: Fig. 2 Cloud lab UV melting validation of the Array Melt method. a Schematic representation of UV melting data collection and analysis using the Emerald Cloud Lab (ECL) platform.

Techniques: Variant Assay, Comparison