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TaKaRa la taq polymerase
La Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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la taq polymerase - by Bioz Stars, 2020-01
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Related Articles

Clone Assay:

Article Title: Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01
Article Snippet: Paragraph title: Cloning of Ferredoxin and Ferredoxin Reductase Genes ... PCR amplification was performed using 1 µM primers and LA Taq polymerase with GC buffer (TaKaRa, Japan).

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: Paragraph title: Cloning of the gene encoding GCR ... The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC.

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: Paragraph title: 3.2. Primers Design, PCR Amplification, Cloning and Sequencing ... PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction.

Amplification:

Article Title: High frequency of Machado-Joseph disease identified in Southeastern Chinese kindreds with spinocerebellar ataxia
Article Snippet: .. The PCR amplification was performed in a total volume of 25 μL containing 0.10 μg of genomic DNA, 0.10 μmol/L of each primer, 50 μmol/L of each dNTP and 1.25 units of LA Taq polymerase with 12.5 μL 2× GC buffer I (TaKaRa, Chiba, Japan). .. PCR products were generated using a Mini-Cycler PCR system (Applied Biosystems, Foster City, CA, USA).

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: SSU rRNA genes were amplified by LA Taq polymerase (Takara Bio, Kusatsu, Japan) using a universal primer set of U530F and U907R ( ). .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa). .. Expression of endogenous FCP was also examined as a positive control using the primer pair FCPExAF2 and FCPExAR2.

Article Title: Speciation in the dark: diversification and biogeography of the deep‐sea gastropod genus Scaphander in the Atlantic Ocean
Article Snippet: DNA was extracted, amplified and sequenced according to the protocol described by Eilertsen & Malaquias , except for some specimens where amplification of 28S failed. .. These were run again using LA Taq polymerase with GC buffer from TaKaRa (TaKaRa Bio, Otsu, Japan).

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: The 2.3-kb fragment containing the 3′ flanking region of ClaPEX6 was amplified by polymerase chain reaction (PCR) with primers PX6AAP (5′-CGCAAGGGGCCCATGGATTGACTGCCACT-3′) and PX6S3 (5′-AGCGACGCGATGCTCAA-3′). .. PCR was performed with LA Taq polymerase with GC-rich buffer (Takara).

Article Title: Molecular Species Identification of Spiny Lobster Phyllosoma Larvae of the Genus Panulirus from the Northwestern Pacific
Article Snippet: .. We observed that LA Taq polymerase with GC buffer (TAKARA Ltd., Kyoto) greatly increased amplification efficiency for the spiny lobster COI compared to the standard Taq DNA polymerase. .. PCR amplification for all specimens was carried out in a 10 μl reaction mixture containing 5 μl of GC buffer, 1 mM of each dNTP, primers (1 μM each for single pair and 0.2μM each for a mixed one), 0.5 unit of LA Taq polymerase, and DNA template.

Article Title: Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01
Article Snippet: .. PCR amplification was performed using 1 µM primers and LA Taq polymerase with GC buffer (TaKaRa, Japan). .. The PCR program was as follows: denaturation at 94°C for 4 min; followed by 30 cycles of 94°C for 1 min, 62°C for 1 min, and 72°C for 2 min; and a final extension of 72°C for 10 min. For cloning FdR genes from ZB01, Primer pairs F1/R1 and F2/R2 were designed based on the known FdR genes in NCBI to amplify the full-length and partial FdR gene fragment respectively in ZB01 in combination with the La Taq DNA polymerase in GCI buffer (TaKaRa, Japan).

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: .. The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Article Title: Characterization of C1-Metabolizing Prokaryotic Communities in Methane Seep Habitats at the Kuroshima Knoll, Southern Ryukyu Arc, by Analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA Genes
Article Snippet: .. Amplification was performed with LA Taq polymerase with GC buffer I (TaKaRa, Tokyo, Japan) for each extracted DNA solution by using the GeneAmp 9600 PCR system (PE Applied Biosystems, Foster City, Calif.). .. The PCR conditions were as follows: denaturation at 96°C for 30 s, annealing at 47°C for 40 s, and extension at 72°C for 50 s for pmoA and mmoX amplification; denaturation at 96°C for 30 s, annealing at 50°C for 40 s, and extension at 72°C for 50 s for mxaF , mcrA , and archaeal 16S rRNA gene amplification; and denaturation at 96°C for 30 s, annealing at 52°C for 30 s, and extension at 72°C for 50 s for bacterial 16S rRNA gene amplification.

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: .. PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. All PCR reactions were performed in an ABI thermal cycler (PE Applied Biosystems, CA, USA).

Article Title: Androgen receptor and monoamine oxidase polymorphism in wild bonobos
Article Snippet: ARQ and ARG were amplified by applying the following primer sets: ARhf (5′-TCCAGAATCTGTTCCAGAGCGTGC-3′) and ARhr (5′-GCTGTGAGGGTTGCTGTTCCTCAT-3′) for ARQ , and ARGF (5′-CAGTGCCGCTATGGGGACCTGGCGA-3′) and ARGR (5′-GGACTGGGATAGGGCACTCTGCTCACC-3′) for ARG ( ). .. A 10 μl PCR reaction contained 2 μl DNA with a concentration of > 150 pg/μl (Step 3 in Table S1 in ), 0.5 μM forward primer, 0.5 μM reverse primer, 0.5 U LA Taq polymerase, 400 μM of each of the dNTP and GC buffer I (TaKaRa, Shiga, Japan).

Article Title: Molecular Phylogenetic Analysis of Archaeal Intron-Containing Genes Coding for rRNA Obtained from a Deep-Subsurface Geothermal Water Pool
Article Snippet: .. SSU rDNAs were amplified by PCR using LA Taq polymerase with GC buffer (TaKaRa, Kyoto, Japan). .. Reaction mixtures in which the concentration of each oligonucleotide primer was 0.1 μM and that of DNA template was 0.1 ng/μl were prepared.

Positive Control:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa). .. Expression of endogenous FCP was also examined as a positive control using the primer pair FCPExAF2 and FCPExAR2.

Synthesized:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: Using 50 ng of total RNA as a template, cDNA was synthesized with the poly-T17 primer and SuperScriptIII reverse transcriptase (Invitrogen). .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa).

DNA Extraction:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: Environmental DNA was extracted from each sediment sample (approximately 5 g) by using a PowerMax Soil DNA Isolation kit (Mo Bio Lab, Carlsbad, CA, United States). .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Construct:

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: Paragraph title: Plasmid Constructs ... PCR was performed with LA Taq polymerase with GC-rich buffer (Takara).

Electrophoresis:

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. PCR products were separated by electrophoresis in a 1.0% agarose gel and purified using a DNA Gel Extraction Kit (Bioteke, Beijing, China).

Incubation:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: After 3 days of incubation, total RNA was extracted from the cells using RNeasy Plant Mini Kit (Qiagen), and treated with TurboDNase Kit (Ambion) to avoid contamination of genomic DNA. .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa).

Article Title: Androgen receptor and monoamine oxidase polymorphism in wild bonobos
Article Snippet: A 10 μl PCR reaction contained 2 μl DNA with a concentration of > 150 pg/μl (Step 3 in Table S1 in ), 0.5 μM forward primer, 0.5 μM reverse primer, 0.5 U LA Taq polymerase, 400 μM of each of the dNTP and GC buffer I (TaKaRa, Shiga, Japan). .. Following the initial incubation at 95 °C for 2 min, PCR amplification was performed for 35 cycles of 95 °C for 30 s, 60 °C (ARQ ) or 65 °C (ARG ) for 1 min and 75 °C for 2 min for AR loci , while 35 cycles (MAin2 ) or 40 cycles (MBin2 ) of 95 °C for 30 s, 50 °C for 1 min and 75 °C for 2 min for MAOA and MAOB loci .

Expressing:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: To examine the expression of PyAph7 , total RNAs were extracted from the ten mutant strains, and cDNAs were prepared from 500 ng of total RNA as described above. .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa).

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: PCR was performed with LA Taq polymerase with GC-rich buffer (Takara). .. Expression of both GFP-PTS1 and green fluorescent protein (GFP) genes was controlled by a 221-bp short promoter region of the melanin gene SCD1 because expression of GFP under the SCD1 short promoter resulted in constitutive GFP fluorescence at all fungal stages examined (Y. Takano, E. Oshiro, and T. Okuno, unpublished data).

Transformation Assay:

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. Purified PCR products were ligated into the T-vector (TaKaRa, Kyoto, Japan) and then transformed into XL-1 blue competent bacteria, according to the method of Wei et al .

Countercurrent Chromatography:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: The Illumina adaptor sequence (5′-ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT-3′; Illumina, San Diego, CA, United States) and Illumina Multiplexing PCR Primer 2.0 (5′-GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC T-3′; Illumina) were added at the 5′ ends of both primers (U530F and U907R). .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: .. The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Ligation:

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Generated:

Article Title: High frequency of Machado-Joseph disease identified in Southeastern Chinese kindreds with spinocerebellar ataxia
Article Snippet: The PCR amplification was performed in a total volume of 25 μL containing 0.10 μg of genomic DNA, 0.10 μmol/L of each primer, 50 μmol/L of each dNTP and 1.25 units of LA Taq polymerase with 12.5 μL 2× GC buffer I (TaKaRa, Chiba, Japan). .. PCR products were generated using a Mini-Cycler PCR system (Applied Biosystems, Foster City, CA, USA).

Article Title: Speciation in the dark: diversification and biogeography of the deep‐sea gastropod genus Scaphander in the Atlantic Ocean
Article Snippet: Molecular phylogenetic analyses and estimation of divergence times Sequences of the mitochondrial genes cytochrome oxidase c subunit I (COI ) and 16S rRNA (16S ) and the nuclear gene 28S rRNA (28S ) of Scaphander were obtained from GenBank (previously generated by Eilertsen & Malaquias, ) and some additional sequences were produced (Table ). .. These were run again using LA Taq polymerase with GC buffer from TaKaRa (TaKaRa Bio, Otsu, Japan).

Polymerase Chain Reaction:

Article Title: High frequency of Machado-Joseph disease identified in Southeastern Chinese kindreds with spinocerebellar ataxia
Article Snippet: .. The PCR amplification was performed in a total volume of 25 μL containing 0.10 μg of genomic DNA, 0.10 μmol/L of each primer, 50 μmol/L of each dNTP and 1.25 units of LA Taq polymerase with 12.5 μL 2× GC buffer I (TaKaRa, Chiba, Japan). .. PCR products were generated using a Mini-Cycler PCR system (Applied Biosystems, Foster City, CA, USA).

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA. ..

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa). .. Expression of endogenous FCP was also examined as a positive control using the primer pair FCPExAF2 and FCPExAR2.

Article Title: Speciation in the dark: diversification and biogeography of the deep‐sea gastropod genus Scaphander in the Atlantic Ocean
Article Snippet: These were run again using LA Taq polymerase with GC buffer from TaKaRa (TaKaRa Bio, Otsu, Japan). .. The PCR reaction volume was 25 μL, comprising 5.35 μL ddH2 O, 12.5 μL GC buffer, 4 μL dNTPs, 1 μL of each primer (10 μM concentration), 0.15 μL Taq and 1 μL DNA template.

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: .. PCR was performed with LA Taq polymerase with GC-rich buffer (Takara). .. The amplified fragment was digested with ApaI and introduced into the ApaI site of pCB5PEX6 to give the plasmid pGDPEX6.

Article Title: Molecular Species Identification of Spiny Lobster Phyllosoma Larvae of the Genus Panulirus from the Northwestern Pacific
Article Snippet: Paragraph title: PCR Amplification ... We observed that LA Taq polymerase with GC buffer (TAKARA Ltd., Kyoto) greatly increased amplification efficiency for the spiny lobster COI compared to the standard Taq DNA polymerase.

Article Title: Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01
Article Snippet: .. PCR amplification was performed using 1 µM primers and LA Taq polymerase with GC buffer (TaKaRa, Japan). .. The PCR program was as follows: denaturation at 94°C for 4 min; followed by 30 cycles of 94°C for 1 min, 62°C for 1 min, and 72°C for 2 min; and a final extension of 72°C for 10 min. For cloning FdR genes from ZB01, Primer pairs F1/R1 and F2/R2 were designed based on the known FdR genes in NCBI to amplify the full-length and partial FdR gene fragment respectively in ZB01 in combination with the La Taq DNA polymerase in GCI buffer (TaKaRa, Japan).

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: .. The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Article Title: Characterization of C1-Metabolizing Prokaryotic Communities in Methane Seep Habitats at the Kuroshima Knoll, Southern Ryukyu Arc, by Analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA Genes
Article Snippet: .. Amplification was performed with LA Taq polymerase with GC buffer I (TaKaRa, Tokyo, Japan) for each extracted DNA solution by using the GeneAmp 9600 PCR system (PE Applied Biosystems, Foster City, Calif.). .. The PCR conditions were as follows: denaturation at 96°C for 30 s, annealing at 47°C for 40 s, and extension at 72°C for 50 s for pmoA and mmoX amplification; denaturation at 96°C for 30 s, annealing at 50°C for 40 s, and extension at 72°C for 50 s for mxaF , mcrA , and archaeal 16S rRNA gene amplification; and denaturation at 96°C for 30 s, annealing at 52°C for 30 s, and extension at 72°C for 50 s for bacterial 16S rRNA gene amplification.

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: .. PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. All PCR reactions were performed in an ABI thermal cycler (PE Applied Biosystems, CA, USA).

Article Title: Androgen receptor and monoamine oxidase polymorphism in wild bonobos
Article Snippet: .. A 10 μl PCR reaction contained 2 μl DNA with a concentration of > 150 pg/μl (Step 3 in Table S1 in ), 0.5 μM forward primer, 0.5 μM reverse primer, 0.5 U LA Taq polymerase, 400 μM of each of the dNTP and GC buffer I (TaKaRa, Shiga, Japan). .. Following the initial incubation at 95 °C for 2 min, PCR amplification was performed for 35 cycles of 95 °C for 30 s, 60 °C (ARQ ) or 65 °C (ARG ) for 1 min and 75 °C for 2 min for AR loci , while 35 cycles (MAin2 ) or 40 cycles (MBin2 ) of 95 °C for 30 s, 50 °C for 1 min and 75 °C for 2 min for MAOA and MAOB loci .

Article Title: Molecular Phylogenetic Analysis of Archaeal Intron-Containing Genes Coding for rRNA Obtained from a Deep-Subsurface Geothermal Water Pool
Article Snippet: .. SSU rDNAs were amplified by PCR using LA Taq polymerase with GC buffer (TaKaRa, Kyoto, Japan). .. Reaction mixtures in which the concentration of each oligonucleotide primer was 0.1 μM and that of DNA template was 0.1 ng/μl were prepared.

Sequencing:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: Paragraph title: Small Subunit (SSU) rRNA Gene Tag Sequencing ... The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Article Title: Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01
Article Snippet: According to the conserved region of the flanking sequence of Fd genes from Streptomyces in NCBI, a pair of primers Fd1 and Rd1 were designed for cloning Fd gene in ZB01. .. PCR amplification was performed using 1 µM primers and LA Taq polymerase with GC buffer (TaKaRa, Japan).

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: Paragraph title: 3.2. Primers Design, PCR Amplification, Cloning and Sequencing ... PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction.

Cellular Antioxidant Activity Assay:

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: .. The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Multiplexing:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: The Illumina adaptor sequence (5′-ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT-3′; Illumina, San Diego, CA, United States) and Illumina Multiplexing PCR Primer 2.0 (5′-GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC T-3′; Illumina) were added at the 5′ ends of both primers (U530F and U907R). .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Fluorescence:

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: PCR was performed with LA Taq polymerase with GC-rich buffer (Takara). .. Expression of both GFP-PTS1 and green fluorescent protein (GFP) genes was controlled by a 221-bp short promoter region of the melanin gene SCD1 because expression of GFP under the SCD1 short promoter resulted in constitutive GFP fluorescence at all fungal stages examined (Y. Takano, E. Oshiro, and T. Okuno, unpublished data).

Mutagenesis:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: To examine the expression of PyAph7 , total RNAs were extracted from the ten mutant strains, and cDNAs were prepared from 500 ng of total RNA as described above. .. The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa).

Labeling:

Article Title: Androgen receptor and monoamine oxidase polymorphism in wild bonobos
Article Snippet: A 10 μl PCR reaction contained 2 μl DNA with a concentration of > 150 pg/μl (Step 3 in Table S1 in ), 0.5 μM forward primer, 0.5 μM reverse primer, 0.5 U LA Taq polymerase, 400 μM of each of the dNTP and GC buffer I (TaKaRa, Shiga, Japan). .. For all loci PCR amplification was followed by a final extension at 74 °C for 10 min. For genotyping we applied the ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, California, USA) according to the manufacturer's instructions by using labeled primers.

Purification:

Article Title: Characterization of C1-Metabolizing Prokaryotic Communities in Methane Seep Habitats at the Kuroshima Knoll, Southern Ryukyu Arc, by Analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA Genes
Article Snippet: Bulk prokaryotic DNA was extracted from 10 g of thawed sediment by using a soil DNA Mega Prep kit (Mo Bio Laboratories, Inc., Solana Beach, Calif.) and then was purified and concentrated as previously described ( ). .. Amplification was performed with LA Taq polymerase with GC buffer I (TaKaRa, Tokyo, Japan) for each extracted DNA solution by using the GeneAmp 9600 PCR system (PE Applied Biosystems, Foster City, Calif.).

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. PCR products were separated by electrophoresis in a 1.0% agarose gel and purified using a DNA Gel Extraction Kit (Bioteke, Beijing, China).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Article Snippet: Paragraph title: RT-PCR and genomic PCR ... The Aph7 mRNA was amplified by PCR with the primers AphExAF1 and AphExAR2 using LA-Taq polymerase with GC buffer 2 (TaKaRa).

Activated Clotting Time Assay:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: The Illumina adaptor sequence (5′-ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT-3′; Illumina, San Diego, CA, United States) and Illumina Multiplexing PCR Primer 2.0 (5′-GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC T-3′; Illumina) were added at the 5′ ends of both primers (U530F and U907R). .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA.

Article Title: The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide-disulfide reductase family
Article Snippet: .. The gene encoding GCR (Accession number, ) was amplified by PCR from Halobacterium sp. NRC-1 genomic DNA with LA Taq™ polymerase in GC-I buffer provided by the manufacturer (Takara Bio, Inc., Otsu, Shiga, Japan) using the following primers: 5′-primer, 5′-GAC GAC GAC AAG ATG ACT ACC GAG CAA CCA CAC-3′; and 3′-primer, 5′-GAG GAG AAG CCC GGT TAC AGC TCG GCC GCG GCG TC. .. The amplified gene was cloned into pET46 (EMD Millipore) by ligation-independent cloning (following the manufacturer’s protocol) under control of an isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible T7 promoter, resulting in incorporation of a His6 tag at the N-terminus of the protein.

Plasmid Preparation:

Article Title: Peroxisomal Metabolic Function Is Required for Appressorium-Mediated Plant Infection by Colletotrichum lagenarium
Article Snippet: Paragraph title: Plasmid Constructs ... PCR was performed with LA Taq polymerase with GC-rich buffer (Takara).

Article Title: Exploring the Electron Transfer Pathway in the Oxidation of Avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01
Article Snippet: PCR amplification was performed using 1 µM primers and LA Taq polymerase with GC buffer (TaKaRa, Japan). .. The resulting 1.3-kb PCR product (fdr 18 gene) using F1/R1 and the 0.6-kb PCR product (partial sequence of fdr 28 gene) using F2/R2 were cloned into the pMD19-T vector system.

Negative Control:

Article Title: Characterization of C1-Metabolizing Prokaryotic Communities in Methane Seep Habitats at the Kuroshima Knoll, Southern Ryukyu Arc, by Analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA Genes
Article Snippet: Amplification was performed with LA Taq polymerase with GC buffer I (TaKaRa, Tokyo, Japan) for each extracted DNA solution by using the GeneAmp 9600 PCR system (PE Applied Biosystems, Foster City, Calif.). .. PCR amplification without a DNA solution was used as a negative control to check for contamination.

Agarose Gel Electrophoresis:

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. PCR products were separated by electrophoresis in a 1.0% agarose gel and purified using a DNA Gel Extraction Kit (Bioteke, Beijing, China).

Produced:

Article Title: Speciation in the dark: diversification and biogeography of the deep‐sea gastropod genus Scaphander in the Atlantic Ocean
Article Snippet: Molecular phylogenetic analyses and estimation of divergence times Sequences of the mitochondrial genes cytochrome oxidase c subunit I (COI ) and 16S rRNA (16S ) and the nuclear gene 28S rRNA (28S ) of Scaphander were obtained from GenBank (previously generated by Eilertsen & Malaquias, ) and some additional sequences were produced (Table ). .. These were run again using LA Taq polymerase with GC buffer from TaKaRa (TaKaRa Bio, Otsu, Japan).

Concentration Assay:

Article Title: Quantitative Viral Community DNA Analysis Reveals the Dominance of Single-Stranded DNA Viruses in Offshore Upper Bathyal Sediment from Tohoku, Japan
Article Snippet: .. The PCR mixture contained LA Taq polymerase (final concentration of 0.1 U μl-1 ), 1 × GC Buffer I (Takara Bio), dNTPs (final concentration of 0.25 mM), each primer (final concentration of 0.2 mM), and template DNA. ..

Article Title: Speciation in the dark: diversification and biogeography of the deep‐sea gastropod genus Scaphander in the Atlantic Ocean
Article Snippet: These were run again using LA Taq polymerase with GC buffer from TaKaRa (TaKaRa Bio, Otsu, Japan). .. The PCR reaction volume was 25 μL, comprising 5.35 μL ddH2 O, 12.5 μL GC buffer, 4 μL dNTPs, 1 μL of each primer (10 μM concentration), 0.15 μL Taq and 1 μL DNA template.

Article Title: Androgen receptor and monoamine oxidase polymorphism in wild bonobos
Article Snippet: .. A 10 μl PCR reaction contained 2 μl DNA with a concentration of > 150 pg/μl (Step 3 in Table S1 in ), 0.5 μM forward primer, 0.5 μM reverse primer, 0.5 U LA Taq polymerase, 400 μM of each of the dNTP and GC buffer I (TaKaRa, Shiga, Japan). .. Following the initial incubation at 95 °C for 2 min, PCR amplification was performed for 35 cycles of 95 °C for 30 s, 60 °C (ARQ ) or 65 °C (ARG ) for 1 min and 75 °C for 2 min for AR loci , while 35 cycles (MAin2 ) or 40 cycles (MBin2 ) of 95 °C for 30 s, 50 °C for 1 min and 75 °C for 2 min for MAOA and MAOB loci .

Article Title: Molecular Phylogenetic Analysis of Archaeal Intron-Containing Genes Coding for rRNA Obtained from a Deep-Subsurface Geothermal Water Pool
Article Snippet: SSU rDNAs were amplified by PCR using LA Taq polymerase with GC buffer (TaKaRa, Kyoto, Japan). .. Reaction mixtures in which the concentration of each oligonucleotide primer was 0.1 μM and that of DNA template was 0.1 ng/μl were prepared.

Gel Extraction:

Article Title: The Complete Mitochondrial Genome of the Pink Stem Borer, Sesamia inferens, in Comparison with Four Other Noctuid Moths
Article Snippet: PCR conditions for amplification of other fragments were as follows: An initial denaturation for 5 min at 95 °C, followed by 35 cycles of denaturation for 1 min at 94 °C, annealing for 1 min at 45–50 °C, elongation for 1–3 min (depending on putative length of the fragments) at 68 °C, and a final extension step of 72 °C for 10 min. For most fragments, LA Taq polymerase (TaKaRa, Kyoto, Japan) was used for the PCR amplification, but for fragments less than 1.3 kb, LA Taq polymerase was replaced by Taq polymerase (TaKaRa, Kyoto, Japan) in the PCR reaction. .. PCR products were separated by electrophoresis in a 1.0% agarose gel and purified using a DNA Gel Extraction Kit (Bioteke, Beijing, China).

Epifluorescence Microscopy:

Article Title: Molecular Phylogenetic Analysis of Archaeal Intron-Containing Genes Coding for rRNA Obtained from a Deep-Subsurface Geothermal Water Pool
Article Snippet: This was in good agreement with the population density determined by epifluorescence microscopy direct count (approximately 102 cells/ml) with 4′,6-diamidino-2-phenylindole (DAPI). .. SSU rDNAs were amplified by PCR using LA Taq polymerase with GC buffer (TaKaRa, Kyoto, Japan).

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    TaKaRa la pcr kit
    La Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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