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TaKaRa la pcr buffer
<t>PCR</t> products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, <t>DNA</t> marker DL 2000; lane M 2 , λ-Hind III DNA marker
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1) Product Images from "Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems"

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems

Journal: Archives of Medical Science : AMS

doi: 10.5114/aoms.2018.79005

PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker
Figure Legend Snippet: PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

Techniques Used: Polymerase Chain Reaction, Recombinant, Marker

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Clone Assay:

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Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl. .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A).

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Amplification:

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DNA Ligation:

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl. .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A).

Synthesized:

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
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Article Title: Molecular characterization of a porcine teschovirus HuN-1 isolate proliferating in PK-15 cell
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Construct:

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: Construction and identification of the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, pFastBacHT A-Der f 4 Using our previously constructed plasmids pET28a(+)-Der f 1 [ ], pET28a(+)-Der f 2 [ ], and pET28b(+)-Der f 4 [ ] as templates, the gene fragments Der f 1, Der f 2, and Der f 4 were amplified by PCR. .. The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl.

Incubation:

Article Title: A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics
Article Snippet: The total mitochondria population, identified by MTG, was bisected such that half of mitochondria events were gated as small (~0.2–0.5 µm) and half gated as large (~0.5–1 µm), as determined by size calibration particles (Fig. ), and samples were collected into individual wells of a 96-well PCR plate containing 1 µl of mitochondrial lysis buffer (10 mM EDTA, 0.5% SDS), immediately overlaid with mineral oil, and incubated at 37 °C for 1 h. Following lysis, plates were stored at −80 °C. .. Single molecule PCR (smPCR) , was performed using Ex Taq DNA Polymerase, Hot Start Version (Takara) with LA PCR buffer (Takara) to generate 331 base pair amplicons.

Formalin-fixed Paraffin-Embedded:

Article Title: Clinicopathologic features and treatment efficacy of Chinese patients with BRAF-mutated metastatic colorectal cancer: a retrospective observational study
Article Snippet: KRAS , NRAS , and BRAF status analysis Genomic DNA of formalin-fixed, paraffin-embedded (FFPE) sections with ≥ 50% tumor cells (if the content of the tumor cells in sections was lower than 50%, the sections would be microdissected) was extracted using an E.Z.N.A.FFPE DNA Kit (Lot. .. Each PCR reaction system consisted of 10 × LA PCR buffer II 2 µL, 2.5 mmol/L dNTPs 2 µL, LA Taq 0.1 µL (DRR200A, TaKaRa, Kusatsu, Shiga, Japan), genomic DNA 2 µL, 10 µmol/L forward primer 0.5 µL, and 10 µmol/L reverse primer 0.5 µL in a final volume of 20 µL.

Transformation Assay:

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA. .. For candidate gene cloning, the targeting PCR fragments were recovered and cloned into the pEASY-T5 simple vector and transformed into T1 competent cells (TransGen Biotech, Beijing, China).

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water. .. The PCR products were gel-purified and ligated into pMD-18-T vector (TaKaRa, Dalian, China), transformed into the E. coli DH5α competent cell.

Positive Control:

Article Title: A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics
Article Snippet: Single molecule PCR (smPCR) , was performed using Ex Taq DNA Polymerase, Hot Start Version (Takara) with LA PCR buffer (Takara) to generate 331 base pair amplicons. .. Of note, the molecules sequenced from positive control mitochondria from the CD-1 mouse contained a G9348A polymorphism when initially screened for the presence of the C57BL/6-specific polymorphism.

Genomic Sequencing:

Article Title: Molecular characterization of a porcine teschovirus HuN-1 isolate proliferating in PK-15 cell
Article Snippet: According to the reference genomic sequences of porcine teschoviruses from GenBank, Eight pairs of primers (as Table showed) was designed to amplify the teschovirus HuN-1 isolate genome. .. PCR was carried out using 2 μl of cDNA and a master mix containing 5 μl 10х LA PCR buffer, 2 μl of each primer (20 μM), 8 μl dNTP mixture (2.5 mM each), 0.5 μl LA Taq (Takara, Dalian, China), and 30.5 μl of ddH2 O in a total reaction volume of 50 μl.

DNA Sequencing:

Article Title: The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida. The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida
Article Snippet: Paragraph title: 2.2. PCR amplification and DNA sequencing ... The cycling was set up with an initial denature step at 94°C for 5 min, followed by 35 cycles (94°C for 30 s, 45–55°C for 1 min, 72°C 1–3 min), and a final extension was executed at 72°C for 10 min. LA‐PCR was carried out in a 20 μl reaction volume containing 12.6 μl ddH2 O, 2 μl 10 × LA‐PCR buffer (Mg2+ plus, Takara), 3.2 μl dNTP mix (2.5 mM each), 0.5 μl each primer (10 μM), 0.2 μl LA Taq DNA polymerase (5 U/μl, Takara), and 1 μl DNA template (50 ng/μl).

Polymerase Chain Reaction:

Article Title: Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)
Article Snippet: .. PCR was performed in a 10-μL reaction volume containing 30 ng gDNA, 0.4 mM of each dNTP, 1.5 mM of Mg2+ , 0.2 μM of each primer, 1 μL of 10× LA PCR buffer (Mg2+ free) and 0.1 μL (5 U/μL) of LA Taq DNA polymerase (TaKaRa Biotechnology, Dalian, China). .. The amplification conditions were 3 min at 95 °C for initial denaturation, followed by 30 cycles of 30 s at 94 °C for denaturation, 30 s at 55 °C for annealing, and 3 min at 72 °C for extension, and a final elongation at 72 °C for 10 min. PCR products were purified with an AxyPrep DNA Gel Extraction kit (Axygen) and directly cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA), and three clones of each PCR product were sequenced.

Article Title: An EAV-HP Insertion in 5? Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Article Snippet: .. First and second PCR amplifications were carried out in a 50 µL reaction volume containing 20 pmol of each primer, 5 µL of 10× LA PCR buffer (Mg2+ plus), 2.5 U of LA Taq (Takara, Dalian, China), 20 mM of each dNTP and 1–2 µL of cDNA or 1st PCR product. .. RACE products were cloned to pMD-18 vector (Takara, Dalian, China), and then sequenced in both directions.

Article Title: A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics
Article Snippet: .. Single molecule PCR (smPCR) , was performed using Ex Taq DNA Polymerase, Hot Start Version (Takara) with LA PCR buffer (Takara) to generate 331 base pair amplicons. .. PCR products were individually sequenced using a 3720xl DNA Analyzer (Applied Biosystems) and analyzed with CodonCode Aligner software (CodonCode Corporation) to determine the strain identity of each mitochondrion based on strain-specific polymorphisms.

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: .. All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O. .. The PCR amplification conditions were as follows: an initial denaturation at 94 °C for 1 min; 35 cycles of 94 °C for 40 s (denaturation), 47–58 °C for 45 s (annealing) and 68 °C for 1 min (extension); followed by a final extension at 72 °C for 10 min. All PCR fragments were successfully amplified using the extracted DNA template, but the CR was cloned into the vector pMD-19T (TaKaRa) and then amplified due to extensive sequence variations.

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: .. The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA. .. The PCR conditions were 95°C for 5 min, followed by 36 cycles of 95°C for 45 s, annealing (55–62 °C) for 50 s, and extension at 72°C for 1 min, with a final extension at 72°C for 12 min. Amplified PCR fragments were separated on a 6% denaturing polyacrylamide gel or a 1.5% agarose gel.

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: .. The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water. .. The PCR program include denaturation at 94°C for 5 min, followed by 35 cycles of 30 s at 94°C, 30 s at 60°C, 60 s at 72°C, and an extension step of 10 min at 72°C.

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: .. The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl. .. Thermal cycling was performed as follows: 2 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 55°C, and 2 min at 72°C; and a final step at 72°C for 10 min. 5 μl of the PCR product were analyzed by agarose gel electrophoresis (1.0%) and visualized with ImageMaster VDS.

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: .. PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA/cDNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers. .. The amplified products were inserted into the pMD18-T vector (Takara) and sequenced using the Sanger method on an ABI3730XL sequence platform (Applied Biosystems, Foster City, CA).

Article Title: The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida. The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida
Article Snippet: Paragraph title: 2.2. PCR amplification and DNA sequencing ... The cycling was set up with an initial denature step at 94°C for 5 min, followed by 35 cycles (94°C for 30 s, 45–55°C for 1 min, 72°C 1–3 min), and a final extension was executed at 72°C for 10 min. LA‐PCR was carried out in a 20 μl reaction volume containing 12.6 μl ddH2 O, 2 μl 10 × LA‐PCR buffer (Mg2+ plus, Takara), 3.2 μl dNTP mix (2.5 mM each), 0.5 μl each primer (10 μM), 0.2 μl LA Taq DNA polymerase (5 U/μl, Takara), and 1 μl DNA template (50 ng/μl).

Article Title: Similar patterns of clonally expanded somatic mtDNA mutations in the colon of heterozygous mtDNA mutator mice and ageing humans
Article Snippet: .. The DNA lysate was taken to a 1:10 dilution and PCR reactions were implemented in 37.5 μl volumes using a mastermix comprising 1× LA PCR buffer (Mg2+ ) (Takara Bio Inc.), 0.2 mM dNTPs, 0.9 μM primers, 5 U LA Taq DNA Polymerase (Takara Bio Inc.) and 3.75 μl of single cell lysate (1:10 dilution). .. The optimal final extension was at 72 °C for 2 min. PCR products were taken to a 1:2 dilution and purified using TSAP (Promega) to remove excess primers, and samples were sequenced using BigDye v3.1 terminator cycle sequencing chemistries on an ABI3130xl Genetic Analyser (Applied Biosystems).

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: .. PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers. .. The amplified products were inserted into the pMD18-T vector (Takara) and sequenced using the Sanger method on an ABI3730XL sequence platform (Applied Biosystems).

Article Title: Clinicopathologic features and treatment efficacy of Chinese patients with BRAF-mutated metastatic colorectal cancer: a retrospective observational study
Article Snippet: .. Each PCR reaction system consisted of 10 × LA PCR buffer II 2 µL, 2.5 mmol/L dNTPs 2 µL, LA Taq 0.1 µL (DRR200A, TaKaRa, Kusatsu, Shiga, Japan), genomic DNA 2 µL, 10 µmol/L forward primer 0.5 µL, and 10 µmol/L reverse primer 0.5 µL in a final volume of 20 µL. ..

Article Title: An EAV-HP Insertion in 5? Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Article Snippet: .. Long-range PCR A long-range PCR amplification with 1B3_5F & 5R primer pair ( ) was performed in volumes of 50 µL containing 5 µL of 10× LA PCR buffer (Mg2+ plus), 2.5 U of LA Taq (Takara, Dalian, China), 20 mM of each dNTP, 20 pmol of each primer and 50 ng genomic DNA. .. The PCR condition was as follow: 94°C for 3 min followed by 33 cycles of 94°C for 30 s, 58°C for 30 s, 72°C for 5 min, and a final extension at 72°C for 10 min.

Article Title: Molecular characterization of a porcine teschovirus HuN-1 isolate proliferating in PK-15 cell
Article Snippet: .. PCR was carried out using 2 μl of cDNA and a master mix containing 5 μl 10х LA PCR buffer, 2 μl of each primer (20 μM), 8 μl dNTP mixture (2.5 mM each), 0.5 μl LA Taq (Takara, Dalian, China), and 30.5 μl of ddH2 O in a total reaction volume of 50 μl. ..

Recombinant:

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: Paragraph title: Construction and identification of the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, pFastBacHT A-Der f 4 ... The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl.

Cellular Antioxidant Activity Assay:

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: Due to the amplification difficulty of the control region (CR) of Lt. halifaxia and Lt. fuscanus mt genomes, one additional pair of primers (F: 5′-TCA ATT TAC TAT TAT ATT TAT TGG AG-3′ and R: 5′-TAA TTT CAA TAG TTT GTC CAT GTA-3′) was designed with online Primer3 ( http://biotools.umassmed.edu/bioapps/primer3_www.cgi ) according to known Culicidae mt genomes and applied to fill the sequence gap of the CR. .. All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O.

Isolation:

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: Paragraph title: RNA isolation and amplification of cDNA ... The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water.

Purification:

Article Title: Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)
Article Snippet: PCR was performed in a 10-μL reaction volume containing 30 ng gDNA, 0.4 mM of each dNTP, 1.5 mM of Mg2+ , 0.2 μM of each primer, 1 μL of 10× LA PCR buffer (Mg2+ free) and 0.1 μL (5 U/μL) of LA Taq DNA polymerase (TaKaRa Biotechnology, Dalian, China). .. The amplification conditions were 3 min at 95 °C for initial denaturation, followed by 30 cycles of 30 s at 94 °C for denaturation, 30 s at 55 °C for annealing, and 3 min at 72 °C for extension, and a final elongation at 72 °C for 10 min. PCR products were purified with an AxyPrep DNA Gel Extraction kit (Axygen) and directly cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA), and three clones of each PCR product were sequenced.

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O. .. All PCR fragments were subsequently purified with a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) and were sequenced using a DNA Sequencer (ABI3730) at Life Technologies™ Company (Shanghai, China) in both directions.

Article Title: The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida. The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida
Article Snippet: The cycling was set up with an initial denature step at 94°C for 5 min, followed by 35 cycles (94°C for 30 s, 45–55°C for 1 min, 72°C 1–3 min), and a final extension was executed at 72°C for 10 min. LA‐PCR was carried out in a 20 μl reaction volume containing 12.6 μl ddH2 O, 2 μl 10 × LA‐PCR buffer (Mg2+ plus, Takara), 3.2 μl dNTP mix (2.5 mM each), 0.5 μl each primer (10 μM), 0.2 μl LA Taq DNA polymerase (5 U/μl, Takara), and 1 μl DNA template (50 ng/μl). .. PCR products were electrophoresed on a 1.0% agarose gel and purified with gel extraction kit (Omega Bio‐Tek) and sequenced with ABI 3730x1 DNA analyzer (Applied Biosystems Inc.).

Article Title: Similar patterns of clonally expanded somatic mtDNA mutations in the colon of heterozygous mtDNA mutator mice and ageing humans
Article Snippet: The DNA lysate was taken to a 1:10 dilution and PCR reactions were implemented in 37.5 μl volumes using a mastermix comprising 1× LA PCR buffer (Mg2+ ) (Takara Bio Inc.), 0.2 mM dNTPs, 0.9 μM primers, 5 U LA Taq DNA Polymerase (Takara Bio Inc.) and 3.75 μl of single cell lysate (1:10 dilution). .. The optimal final extension was at 72 °C for 2 min. PCR products were taken to a 1:2 dilution and purified using TSAP (Promega) to remove excess primers, and samples were sequenced using BigDye v3.1 terminator cycle sequencing chemistries on an ABI3130xl Genetic Analyser (Applied Biosystems).

Sequencing:

Article Title: Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)
Article Snippet: Determination of the CsuPLE1.1 Copy at the Same Locus in Different Individuals Based on the flanking sequence of CsuPLE1.1 , PCR with the primer pairs Flk-PBF (5′-TAACTAAGGTTCGCTGATGAC-3′) and Flk-PBR (5′-GATGCGCCTATCTATTTCG-3′) was carried out to obtain the CsuPLE1.1 copy in different C. suppressalis individuals ( A). .. PCR was performed in a 10-μL reaction volume containing 30 ng gDNA, 0.4 mM of each dNTP, 1.5 mM of Mg2+ , 0.2 μM of each primer, 1 μL of 10× LA PCR buffer (Mg2+ free) and 0.1 μL (5 U/μL) of LA Taq DNA polymerase (TaKaRa Biotechnology, Dalian, China).

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: Paragraph title: Mt genome sequencing, assembly and annotation ... All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O.

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: Candidate Gene Cloning and Development of CAPS and InDel Markers Eight sets of primer pairs were designed using Primer Premier 5 software based on the sequence of 7AS_4248784 and its transcript Traes_7AS_378A12AA9.1 on 7AS, which were obtained by BLAST and functional annotation and gene/transcript set/pathway enrichment analyses of SLAF65386 at a hot region on chromosome 7A. .. The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA.

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: According to the mRNA sequence of the Gallus gallus Syt1 gene (Genbank accession no. NM_205171.1), a pair of primers (Syt1-F/Syt1-R) was designed to obtain a partial goose Syt1 gene sequence (primers shown in Table ) by using Primer Premier 6.0 software (Primer Biosoft International, Palo Alto, California, USA). .. The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water.

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: The primers (Supplementary Material: Table S1), including Bror-walking-1, Bror-walking-2, and Bror-walking-3, were designed based on part I sequence. .. PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA/cDNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers.

Article Title: Similar patterns of clonally expanded somatic mtDNA mutations in the colon of heterozygous mtDNA mutator mice and ageing humans
Article Snippet: Paragraph title: MtDNA sequencing of individual colonic crypts ... The DNA lysate was taken to a 1:10 dilution and PCR reactions were implemented in 37.5 μl volumes using a mastermix comprising 1× LA PCR buffer (Mg2+ ) (Takara Bio Inc.), 0.2 mM dNTPs, 0.9 μM primers, 5 U LA Taq DNA Polymerase (Takara Bio Inc.) and 3.75 μl of single cell lysate (1:10 dilution).

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: Validation of the SNP (C952 to T952 ) in Different White and Orange Cultivars and F2 Populations To validate the SNP (C952 to T952 ), the DNA sequence that contains this site was PCR amplified in different white and orange cultivars and F2 populations using the specific primer (OR-SNP952 , Supplementary Material: Table S1). .. PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers.

Lysis:

Article Title: A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics
Article Snippet: Lysed samples were diluted across 32 PCR wells to both prevent inhibitory effects of mitochondrial lysis buffer on amplification and to ensure templates were individually segregated into separate wells. .. Single molecule PCR (smPCR) , was performed using Ex Taq DNA Polymerase, Hot Start Version (Takara) with LA PCR buffer (Takara) to generate 331 base pair amplicons.

Nested PCR:

Article Title: An EAV-HP Insertion in 5? Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Article Snippet: 5′ and 3′ UTR of SLCO1B3 gene transcripts were amplified by nested PCR with gene specific ( ) and adaptor primers ( ) for the first and second amplifications of 5′ and 3′ UTR respectively. .. First and second PCR amplifications were carried out in a 50 µL reaction volume containing 20 pmol of each primer, 5 µL of 10× LA PCR buffer (Mg2+ plus), 2.5 U of LA Taq (Takara, Dalian, China), 20 mM of each dNTP and 1–2 µL of cDNA or 1st PCR product.

Chloramphenicol Acetyltransferase Assay:

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: Due to the amplification difficulty of the control region (CR) of Lt. halifaxia and Lt. fuscanus mt genomes, one additional pair of primers (F: 5′-TCA ATT TAC TAT TAT ATT TAT TGG AG-3′ and R: 5′-TAA TTT CAA TAG TTT GTC CAT GTA-3′) was designed with online Primer3 ( http://biotools.umassmed.edu/bioapps/primer3_www.cgi ) according to known Culicidae mt genomes and applied to fill the sequence gap of the CR. .. All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O.

Plasmid Preparation:

Article Title: Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)
Article Snippet: PCR was performed in a 10-μL reaction volume containing 30 ng gDNA, 0.4 mM of each dNTP, 1.5 mM of Mg2+ , 0.2 μM of each primer, 1 μL of 10× LA PCR buffer (Mg2+ free) and 0.1 μL (5 U/μL) of LA Taq DNA polymerase (TaKaRa Biotechnology, Dalian, China). .. The amplification conditions were 3 min at 95 °C for initial denaturation, followed by 30 cycles of 30 s at 94 °C for denaturation, 30 s at 55 °C for annealing, and 3 min at 72 °C for extension, and a final elongation at 72 °C for 10 min. PCR products were purified with an AxyPrep DNA Gel Extraction kit (Axygen) and directly cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA), and three clones of each PCR product were sequenced.

Article Title: An EAV-HP Insertion in 5? Flanking Region of SLCO1B3 Causes Blue Eggshell in the Chicken
Article Snippet: First and second PCR amplifications were carried out in a 50 µL reaction volume containing 20 pmol of each primer, 5 µL of 10× LA PCR buffer (Mg2+ plus), 2.5 U of LA Taq (Takara, Dalian, China), 20 mM of each dNTP and 1–2 µL of cDNA or 1st PCR product. .. RACE products were cloned to pMD-18 vector (Takara, Dalian, China), and then sequenced in both directions.

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O. .. The PCR amplification conditions were as follows: an initial denaturation at 94 °C for 1 min; 35 cycles of 94 °C for 40 s (denaturation), 47–58 °C for 45 s (annealing) and 68 °C for 1 min (extension); followed by a final extension at 72 °C for 10 min. All PCR fragments were successfully amplified using the extracted DNA template, but the CR was cloned into the vector pMD-19T (TaKaRa) and then amplified due to extensive sequence variations.

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA. .. For candidate gene cloning, the targeting PCR fragments were recovered and cloned into the pEASY-T5 simple vector and transformed into T1 competent cells (TransGen Biotech, Beijing, China).

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water. .. The PCR products were gel-purified and ligated into pMD-18-T vector (TaKaRa, Dalian, China), transformed into the E. coli DH5α competent cell.

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA/cDNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers. .. The amplified products were inserted into the pMD18-T vector (Takara) and sequenced using the Sanger method on an ABI3730XL sequence platform (Applied Biosystems, Foster City, CA).

Article Title: Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Article Snippet: PCR reaction was performed in a 50 μ l reaction system containing 20 ng template DNA, 5.0 μ l 10 × LA PCR buffer, 3.0 μ l 2.5 mM dNTPs, 2 U LA Taq polymerase (Takara), and 1 μ l 10 μ M of primers. .. The amplified products were inserted into the pMD18-T vector (Takara) and sequenced using the Sanger method on an ABI3730XL sequence platform (Applied Biosystems).

Software:

Article Title: A nanoscale, multi-parametric flow cytometry-based platform to study mitochondrial heterogeneity and mitochondrial DNA dynamics
Article Snippet: Single molecule PCR (smPCR) , was performed using Ex Taq DNA Polymerase, Hot Start Version (Takara) with LA PCR buffer (Takara) to generate 331 base pair amplicons. .. PCR products were individually sequenced using a 3720xl DNA Analyzer (Applied Biosystems) and analyzed with CodonCode Aligner software (CodonCode Corporation) to determine the strain identity of each mitochondrion based on strain-specific polymorphisms.

Article Title: The complete mt genomes of Lutzia halifaxia, Lt. fuscanus and Culex pallidothorax (Diptera: Culicidae) and comparative analysis of 16 Culex and Lutzia mt genome sequences
Article Snippet: All PCR amplifications were performed in 25 μl reactions containing 4 μl of dNTPs, 1 μl of each primer, 2.5 μl of 10× LA PCR buffer I, 1–2 μl of DNA template, 0.25 μl of LA Taq polymerase (TaKaRa, Dalian, China) and 14.25–15.25 μl ddH2 O. .. The obtained sequences were assembled using DNAMANx software.

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: Candidate Gene Cloning and Development of CAPS and InDel Markers Eight sets of primer pairs were designed using Primer Premier 5 software based on the sequence of 7AS_4248784 and its transcript Traes_7AS_378A12AA9.1 on 7AS, which were obtained by BLAST and functional annotation and gene/transcript set/pathway enrichment analyses of SLAF65386 at a hot region on chromosome 7A. .. The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA.

Article Title: Molecular cloning and expression analysis of the Synaptotagmin-1 gene in the hypothalamus and pituitary of Huoyan goose during different stages of the egg-laying cycle
Article Snippet: According to the mRNA sequence of the Gallus gallus Syt1 gene (Genbank accession no. NM_205171.1), a pair of primers (Syt1-F/Syt1-R) was designed to obtain a partial goose Syt1 gene sequence (primers shown in Table ) by using Primer Premier 6.0 software (Primer Biosoft International, Palo Alto, California, USA). .. The 50 μl reaction consisted of 1 μl of cDNA, 8 μl of deoxynucleoside triphosphate mix (2.5 mmol/L each dATP, dGTP, dCTP and dTTP), 2 μl of each primer (10 μmol/l), 5 μl of 10 × LA PCR Buffer, 0.5 μl of 5U/μl LA Taq™ (TaKaRa, Dalian, China), and 31.5 μl sterile MilliQ water.

Article Title: Similar patterns of clonally expanded somatic mtDNA mutations in the colon of heterozygous mtDNA mutator mice and ageing humans
Article Snippet: The DNA lysate was taken to a 1:10 dilution and PCR reactions were implemented in 37.5 μl volumes using a mastermix comprising 1× LA PCR buffer (Mg2+ ) (Takara Bio Inc.), 0.2 mM dNTPs, 0.9 μM primers, 5 U LA Taq DNA Polymerase (Takara Bio Inc.) and 3.75 μl of single cell lysate (1:10 dilution). .. The sequence for each crypt was aligned to the C57Bl/6J mouse reference sequence (GenBank Accession number NC_005089) and the consensus DNA sequence for that mouse using SeqScape software (Applied Biosystems) to determine the somatic mtDNA point mutations that accumulated in the crypts over time.

Functional Assay:

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: Candidate Gene Cloning and Development of CAPS and InDel Markers Eight sets of primer pairs were designed using Primer Premier 5 software based on the sequence of 7AS_4248784 and its transcript Traes_7AS_378A12AA9.1 on 7AS, which were obtained by BLAST and functional annotation and gene/transcript set/pathway enrichment analyses of SLAF65386 at a hot region on chromosome 7A. .. The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA.

Agarose Gel Electrophoresis:

Article Title: Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA
Article Snippet: The PCR reactions (total volume, 10 μL) included 0.25 μM each primer, 0.25 mM dNTP, 0.5 unit LA Taq , 1 μL 10 × LA PCR buffer (Takara, Dalian, China) and 100 ng of genomic DNA. .. The PCR conditions were 95°C for 5 min, followed by 36 cycles of 95°C for 45 s, annealing (55–62 °C) for 50 s, and extension at 72°C for 1 min, with a final extension at 72°C for 12 min. Amplified PCR fragments were separated on a 6% denaturing polyacrylamide gel or a 1.5% agarose gel.

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl. .. Thermal cycling was performed as follows: 2 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 55°C, and 2 min at 72°C; and a final step at 72°C for 10 min. 5 μl of the PCR product were analyzed by agarose gel electrophoresis (1.0%) and visualized with ImageMaster VDS.

Article Title: The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida. The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida
Article Snippet: The cycling was set up with an initial denature step at 94°C for 5 min, followed by 35 cycles (94°C for 30 s, 45–55°C for 1 min, 72°C 1–3 min), and a final extension was executed at 72°C for 10 min. LA‐PCR was carried out in a 20 μl reaction volume containing 12.6 μl ddH2 O, 2 μl 10 × LA‐PCR buffer (Mg2+ plus, Takara), 3.2 μl dNTP mix (2.5 mM each), 0.5 μl each primer (10 μM), 0.2 μl LA Taq DNA polymerase (5 U/μl, Takara), and 1 μl DNA template (50 ng/μl). .. PCR products were electrophoresed on a 1.0% agarose gel and purified with gel extraction kit (Omega Bio‐Tek) and sequenced with ABI 3730x1 DNA analyzer (Applied Biosystems Inc.).

DNA Purification:

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl. .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A).

Gel Extraction:

Article Title: Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)
Article Snippet: PCR was performed in a 10-μL reaction volume containing 30 ng gDNA, 0.4 mM of each dNTP, 1.5 mM of Mg2+ , 0.2 μM of each primer, 1 μL of 10× LA PCR buffer (Mg2+ free) and 0.1 μL (5 U/μL) of LA Taq DNA polymerase (TaKaRa Biotechnology, Dalian, China). .. The amplification conditions were 3 min at 95 °C for initial denaturation, followed by 30 cycles of 30 s at 94 °C for denaturation, 30 s at 55 °C for annealing, and 3 min at 72 °C for extension, and a final elongation at 72 °C for 10 min. PCR products were purified with an AxyPrep DNA Gel Extraction kit (Axygen) and directly cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA), and three clones of each PCR product were sequenced.

Article Title: The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida. The first complete mitochondrial genome of the Mariana Trench Freyastera benthophila (Asteroidea: Brisingida: Brisingidae) allows insights into the deep‐sea adaptive evolution of Brisingida
Article Snippet: The cycling was set up with an initial denature step at 94°C for 5 min, followed by 35 cycles (94°C for 30 s, 45–55°C for 1 min, 72°C 1–3 min), and a final extension was executed at 72°C for 10 min. LA‐PCR was carried out in a 20 μl reaction volume containing 12.6 μl ddH2 O, 2 μl 10 × LA‐PCR buffer (Mg2+ plus, Takara), 3.2 μl dNTP mix (2.5 mM each), 0.5 μl each primer (10 μM), 0.2 μl LA Taq DNA polymerase (5 U/μl, Takara), and 1 μl DNA template (50 ng/μl). .. PCR products were electrophoresed on a 1.0% agarose gel and purified with gel extraction kit (Omega Bio‐Tek) and sequenced with ABI 3730x1 DNA analyzer (Applied Biosystems Inc.).

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  • 90
    TaKaRa la taq tm kit
    La Taq Tm Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 70 article reviews
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    la taq tm kit - by Bioz Stars, 2020-01
    90/100 stars
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    82
    TaKaRa gc buffer i
    Gc Buffer I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc buffer i/product/TaKaRa
    Average 82 stars, based on 228 article reviews
    Price from $9.99 to $1999.99
    gc buffer i - by Bioz Stars, 2020-01
    82/100 stars
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