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TaKaRa la pcr buffer
<t>PCR</t> products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, <t>DNA</t> marker DL 2000; lane M 2 , λ-Hind III DNA marker
La Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems"

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems

Journal: Archives of Medical Science : AMS

doi: 10.5114/aoms.2018.79005

PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker
Figure Legend Snippet: PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

Techniques Used: Polymerase Chain Reaction, Recombinant, Marker

2) Product Images from "Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems"

Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems

Journal: Archives of Medical Science : AMS

doi: 10.5114/aoms.2018.79005

PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker
Figure Legend Snippet: PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

Techniques Used: Polymerase Chain Reaction, Recombinant, Marker

Related Articles

DNA Extraction:

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Amplification:

Article Title: Effect of Nano-SiO2 on Expression and Aberrant Methylation of Imprinted Genes in Lung and Testis
Article Snippet: .. Each DNA sample was amplified by PCR as follows: 2.5 μl 10 × PCR buffer PCR reaction mix with 500 ng the bisulfite-treated DNA, 0.5 μl each of forward and reverse primers, 0.5 μl dNTP Mix, 0.5 μl rTaq (500 U, dNTP, Mg2+ ) (Takara Bio, Tokyo, Japan), addition of ddH2 O up to a volume of 25 μl. .. A549 cells were purchased from ATCC (Manassas, VA, USA) and were cultured in 1640 supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2 in a humidified incubator.

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Transferring:

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Polymerase Chain Reaction:

Article Title: Expression and Molecular Evolution of Two DREB1 Genes in Black Poplar (Populus nigra)
Article Snippet: .. To amplify genomic DNA, reactions were carried out in a volume of 50 µl containing 50 ng of template DNA, 0.2 mM of each dNTP, 0.5 µM of each primer, 1× PCR buffer plus MgCl2 , and 0.5 U LA-Taq polymerase (TaKaRa, Dalian, China). .. All PCR reactions were run on a PE 9700 Thermal Cycler (Applied Biosystems, Foster City, CA, USA).

Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
Article Snippet: .. 10 μM forward primer (F1 or F2, if desired) 10 μM reverse primer (R1 or R2, if desired) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57 [ ] .. Use reagents in section 2.4, but use the heterozygous yfg1::dpl200/YFG1 strain as the transformation recipient instead of YFG1/YFG1 strain BWP17.

Article Title: Identification of novel SNPs of ABCD1, ABCD2, ABCD3, and ABCD4 genes in patients with X-linked adrenoleukodystrophy (ALD) based on comprehensive resequencing and association studies with ALD phenotypes
Article Snippet: .. The PCR conditions were as follows: 94°C for 1 min, followed by five cycles consisting of 94°C for 30 s, 62°C for 30 s, and 68°C for 2 min; five cycles consisting of 94°C for 30 s, 60°C for 30 s, and 68°C for 2 min; and 25 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 68°C for 2 min, followed by a final extension at 68°C for 7 min, using the LA Taq with GC Buffer PCR system (Takara Bio, Otsu, Shiga, Japan). ..

Article Title: Effect of Nano-SiO2 on Expression and Aberrant Methylation of Imprinted Genes in Lung and Testis
Article Snippet: .. Each DNA sample was amplified by PCR as follows: 2.5 μl 10 × PCR buffer PCR reaction mix with 500 ng the bisulfite-treated DNA, 0.5 μl each of forward and reverse primers, 0.5 μl dNTP Mix, 0.5 μl rTaq (500 U, dNTP, Mg2+ ) (Takara Bio, Tokyo, Japan), addition of ddH2 O up to a volume of 25 μl. .. A549 cells were purchased from ATCC (Manassas, VA, USA) and were cultured in 1640 supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2 in a humidified incubator.

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
Article Snippet: .. 10 μM forward primer (F1) 10 μM reverse primer (R1) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57, Pubmed accession number: [ ] .. C. albicans strain BWP17 [ ] YPD+Uri (80 μg/μL) plates: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 2% bacto-agar, 80 μg/μL of uridine YPD+Uri (80 μg/μL) liquid medium: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 80 μg/μL of uridine Ura-blister cassette PCR product LATE (0.1M LiOAc in 1X TE buffer): 0.372g/L 1mM EDTA disodium salt, 1.21g/L Tris, 10.2g/L Lithium acetate, pH 7.5 Calf thymus DNA (~10 mg/mL) (Sigma D8661-1ML) PLATE (8 mL 50% w/v PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc): For 50% w/v PEG3350 dissolve 50g PEG3350 in 100 mL (final volume) of distilled water and filter sterilize after mixing; For 10x TE 3.72g/L 1mM EDTA disodium salt, 12.1g 10mM TRIS, pH 7.5; For 1M LiOAc 102g/L Lithium acetate pH 7.5.

Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
Article Snippet: .. 10 μM Comp forward primer (CF) 10 μM comp reverse primer (CR) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Genomic DNA: Reference strain ( see ) .. S. cerevisiae BY4741Δ trp strain [ ] YPD plates (see item 2, section 2.4) YPD liquid medium (see item 3, section 2.4) pDDB78 plasmid, Pubmed accession number pending [ ] PCR product: wild type gene amplified using complementation primers Restriction Enzymes NotI and EcoRI (New England Biolabs) 10X NE Buffer 3 (New England Biolabs) 100X BSA (New England Biolabs) Calf thymus DNA (~10 mg/mL) (Sigma) PLATE (8 mL 50% PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc) CSM-TRP plates: 2% glucose, 0.67% yeast nitrogen base (without amino acids), 0.074% of CSM-TRP dropout medium (MP Biomedicals, LLC), 2% bacto-agar CSM-TRP liquid medium: 2% glucose, 0.67% yeast nitrogen base (without amino acids), 0.074% of CSM-URA dropout medium (MP Biomedicals, LLC) Plasmid DNA Miniprep kit (Fermentas GeneJET™ Plasmid Miniprep Kit) Acid washed glass beads (size:425–600μm)

Incubation:

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Sequencing:

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Lysis:

Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

Plasmid Preparation:

Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
Article Snippet: .. 10 μM forward primer (F1 or F2, if desired) 10 μM reverse primer (R1 or R2, if desired) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57 [ ] .. Use reagents in section 2.4, but use the heterozygous yfg1::dpl200/YFG1 strain as the transformation recipient instead of YFG1/YFG1 strain BWP17.

Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
Article Snippet: .. 10 μM forward primer (F1) 10 μM reverse primer (R1) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57, Pubmed accession number: [ ] .. C. albicans strain BWP17 [ ] YPD+Uri (80 μg/μL) plates: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 2% bacto-agar, 80 μg/μL of uridine YPD+Uri (80 μg/μL) liquid medium: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 80 μg/μL of uridine Ura-blister cassette PCR product LATE (0.1M LiOAc in 1X TE buffer): 0.372g/L 1mM EDTA disodium salt, 1.21g/L Tris, 10.2g/L Lithium acetate, pH 7.5 Calf thymus DNA (~10 mg/mL) (Sigma D8661-1ML) PLATE (8 mL 50% w/v PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc): For 50% w/v PEG3350 dissolve 50g PEG3350 in 100 mL (final volume) of distilled water and filter sterilize after mixing; For 10x TE 3.72g/L 1mM EDTA disodium salt, 12.1g 10mM TRIS, pH 7.5; For 1M LiOAc 102g/L Lithium acetate pH 7.5.

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    TaKaRa la pcr buffer ii
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Pcr Buffer Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr buffer ii/product/TaKaRa
    Average 91 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    la pcr buffer ii - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    86
    TaKaRa la pcr reaction buffer ii
    <t>PCR</t> amplicons from mitochondrial <t>DNA</t> of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .
    La Pcr Reaction Buffer Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr reaction buffer ii/product/TaKaRa
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    la pcr reaction buffer ii - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    90
    TaKaRa la pcr buffer
    <t>PCR</t> products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, <t>DNA</t> marker DL 2000; lane M 2 , λ-Hind III DNA marker
    La Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr buffer/product/TaKaRa
    Average 90 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    la pcr buffer - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Journal: International Journal of Molecular Sciences

    Article Title: Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

    doi: 10.3390/ijms14036436

    Figure Lengend Snippet: Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Article Snippet: PCR was performed with 0.06 units/μL LA Taq Hot-Start Version (Takara, Shiga, Japan), 0.03 units/μL KOD polymerase (Toyobo, Tokyo, Japan) or 40 nM A. aeolicus DnaE, in 1× Takara LA PCR buffer II (Takara, Shiga, Japan) containing 20 nM template DNA; 400 nM primers; 400 μM dATP, dTTP, dCTP and dGTP and various concentrations of Tth MutS or Aae MutS.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay

    Cytogenetic, FISH, and PCR analyses of the metastasis from the endometrial stromal sarcoma. A) Partial karyotype showing chromosome aberrations der(1)t(1;6)(p34;p21) and der(6)t(1;6)(p34;p21) together with the corresponding normal chromosomes; breakpoint position are indicated by arrows. B) FISH using BAC RP11-508M23 (green signal) from 1p34 containing the MEAF6 gene and a pool of the RP11-600P03 and RP11-436J22 BACs (red signal) from 6p21 containing the PHF1 gene. A part of the probe from 6p21 (red signal) has moved to the derivative chromosome 1, while the entire probe containing MEAF6 has moved to the derivative chromosome 6. The data suggest that the functional fusion gene is generated on the der(6). C) G-banding of the metaphase spread shown in (B). D) Amplification of a 1 kb cDNA in the 5′-RACE analysis (R) using reverse PHF1-721R and PHF1-526R primers and the universal forward primers. E) Partial sequence chromatograms of the 1 kb cDNA fragment showing the junctions (arrow) of MEAF6-PHF1 chimeric transcript (upper) and genomic hybrid DNA fragment (lower). F) RT-PCR and genomic PCR using specific MEAF6 and PHF1 primers. Lane 1: Amplification of MEAF6-PHF1 cDNA fragment with MEAF6-322F/PHF1-380R primers, lane 2: Amplification of MEAF6 transcript with MEAF6-15F/MEAF6-700R, lane 3: PHF1-18F/MEAF6-729R primer set did not amplify the reciprocal PHF1-MEAF6 cDNA, lane 4: Amplification of PHF1 transcript with PHF1-18F/PHF1-327R primers, lane 5: Amplification of MEAF6-PHF1 genomic hybrid DNA fragment with MEAF6-371F/PHF1-302R primer combination. M, 1 kb DNA ladder.

    Journal: PLoS ONE

    Article Title: Novel Fusion of MYST/Esa1-Associated Factor 6 and PHF1 in Endometrial Stromal Sarcoma

    doi: 10.1371/journal.pone.0039354

    Figure Lengend Snippet: Cytogenetic, FISH, and PCR analyses of the metastasis from the endometrial stromal sarcoma. A) Partial karyotype showing chromosome aberrations der(1)t(1;6)(p34;p21) and der(6)t(1;6)(p34;p21) together with the corresponding normal chromosomes; breakpoint position are indicated by arrows. B) FISH using BAC RP11-508M23 (green signal) from 1p34 containing the MEAF6 gene and a pool of the RP11-600P03 and RP11-436J22 BACs (red signal) from 6p21 containing the PHF1 gene. A part of the probe from 6p21 (red signal) has moved to the derivative chromosome 1, while the entire probe containing MEAF6 has moved to the derivative chromosome 6. The data suggest that the functional fusion gene is generated on the der(6). C) G-banding of the metaphase spread shown in (B). D) Amplification of a 1 kb cDNA in the 5′-RACE analysis (R) using reverse PHF1-721R and PHF1-526R primers and the universal forward primers. E) Partial sequence chromatograms of the 1 kb cDNA fragment showing the junctions (arrow) of MEAF6-PHF1 chimeric transcript (upper) and genomic hybrid DNA fragment (lower). F) RT-PCR and genomic PCR using specific MEAF6 and PHF1 primers. Lane 1: Amplification of MEAF6-PHF1 cDNA fragment with MEAF6-322F/PHF1-380R primers, lane 2: Amplification of MEAF6 transcript with MEAF6-15F/MEAF6-700R, lane 3: PHF1-18F/MEAF6-729R primer set did not amplify the reciprocal PHF1-MEAF6 cDNA, lane 4: Amplification of PHF1 transcript with PHF1-18F/PHF1-327R primers, lane 5: Amplification of MEAF6-PHF1 genomic hybrid DNA fragment with MEAF6-371F/PHF1-302R primer combination. M, 1 kb DNA ladder.

    Article Snippet: The 50 µL PCR volume contained 2 µL of cDNA, 1x LA PCR Buffer II (Mg2+ plus), 0.4 mM of each dNTP, 2.5 unit TaKaRa LA Taq (TaKaRA), and 0.6 µM of each of the forward and reverse primers.

    Techniques: Fluorescence In Situ Hybridization, Polymerase Chain Reaction, BAC Assay, Functional Assay, Generated, Amplification, Sequencing, Reverse Transcription Polymerase Chain Reaction

    PCR amplicons from mitochondrial DNA of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .

    Journal: BMC Genomics

    Article Title: Evolution of multipartite mitochondrial genomes in the booklice of the genus Liposcelis (Psocoptera)

    doi: 10.1186/1471-2164-15-861

    Figure Lengend Snippet: PCR amplicons from mitochondrial DNA of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .

    Article Snippet: Each long PCR reaction is 25 μL in volume, containing 1.0 μL each of forward primer (10 μM) and reverse primer (10 μM), 4.0 μL of dNTPs mix (each 2.5 mM), 1.0 μL of template DNA, 2.5 μL MgCl2 (25 mM), 2.5 μL of 10 × LA PCR reaction buffer II, 12.75 μL ddH2 O and 0.25 μL LA Taq DNA polymerase (5 U/μL, Takara).

    Techniques: Polymerase Chain Reaction, Marker

    PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

    Journal: Archives of Medical Science : AMS

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems

    doi: 10.5114/aoms.2018.79005

    Figure Lengend Snippet: PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

    Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl.

    Techniques: Polymerase Chain Reaction, Recombinant, Marker