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TaKaRa la pcr buffer ll
Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
La Pcr Buffer Ll, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
la pcr buffer ll - by Bioz Stars, 2020-01
89/100 stars

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1) Product Images from "Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics"

Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics

Journal: British Journal of Cancer

doi: 10.1038/bjc.2014.176

Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
Figure Legend Snippet: Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.

Techniques Used: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay

Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.
Figure Legend Snippet: Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.

Techniques Used: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay

Related Articles

Polymerase Chain Reaction:

Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics
Article Snippet: .. The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys). .. Results and discussion We tested the 3DPCR error rate using the first PCR amplification corresponding to 235 bp.

Nested PCR:

Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics
Article Snippet: Nested PCR was performed with 1/50 of the first round, conditions were 80–87 °C for 5 min, followed by 35 cycles (80–87 °C for 30 s, 60 °C for 30 s and 72 °C for 10 min) and finally 20 min at 72 °C with the following primers: 5′β catin (5′-ACATGGCCATGGAACCAGACAGA-3′) and 3′β catin (5′-GTTCTTGAGTGAAGGACTGAGAA-3′). .. The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

Amplification:

Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics
Article Snippet: Conditions of amplification of the β -catenin were 95 °C for 5 min, followed by 35 cycles (95 °C for 30 s, 60 °C for 30 s and 72 °C for 10 min) and finally 20 min at 72 °C with the following primers: 5′β catout (5′-AGCTGATTTGATGGAGTTGGACA-3′) and 3′β catout (5′-CCAGCTACTTGTTCTTGAGTGAA-3′). .. The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

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    TaKaRa la pcr buffer ll
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    La Pcr Buffer Ll, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr buffer ll/product/TaKaRa
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    la pcr buffer ll - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    79
    TaKaRa 10x la pcr buffer ll
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    10x La Pcr Buffer Ll, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x la pcr buffer ll/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x la pcr buffer ll - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results


    Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.

    Journal: British Journal of Cancer

    Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics

    doi: 10.1038/bjc.2014.176

    Figure Lengend Snippet: Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.

    Article Snippet: The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay

    Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.

    Journal: British Journal of Cancer

    Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics

    doi: 10.1038/bjc.2014.176

    Figure Lengend Snippet: Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.

    Article Snippet: The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay