la pcr buffer ii  (TaKaRa)

 
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    Name:
    TaKaRa LA Taq DNA Polymerase
    Description:
    TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase with 3 →5 exonuclease activity to enable PCR amplification of very long DNA templates long range PCR This mixture of enzymes allows for long and accurate LA PCR of targets from a variety of templates including genomic DNA LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II with or without Mg2 and dNTPs
    Catalog Number:
    rr002a
    Price:
    None
    Size:
    125 Units
    Category:
    LA Taq DNA polymerase LA Taq products Long range PCR PCR
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    Structured Review

    TaKaRa la pcr buffer ii
    TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase with 3 →5 exonuclease activity to enable PCR amplification of very long DNA templates long range PCR This mixture of enzymes allows for long and accurate LA PCR of targets from a variety of templates including genomic DNA LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II with or without Mg2 and dNTPs
    https://www.bioz.com/result/la pcr buffer ii/product/TaKaRa
    Average 99 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    la pcr buffer ii - by Bioz Stars, 2020-09
    99/100 stars

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    Polymerase Chain Reaction:

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. PCR conditions were investigated including the variation of the annealing temperature from 57 to 64°C, the concentration of primer sets from 0.08 to 0.56 μM, the concentration of La Taq DNA polymerase (TaKaRa, Dalian, China) from 0.75 to 1.75 U, and the concentration of PCR buffer (10×) and the dNTPs (2.5 mM) from 1.5 to 3 μL and 2 to 6 μL, respectively. ..

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. Optimization of the Multiplex PCR After optimization, the optimum multiplex PCR assay was performed in a final volume of 25 μL, containing 2.5 μL of 10× PCR La buffer, 4 μL of dNTPs at 2.5 mM, 1.25 U of La Taq DNA polymerase, 0.32 μM of each primer, and 2 μL of the DNA template. ..

    Article Title: Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)
    Article Snippet: .. The L. garvieae EPS synthesis gene cluster, as described in L. garvieae Lg2 (Miyauchi et al. ), was amplified in isolates A1–A3, A5, A6, A11 and A12 using the TaKaRa LA PCR Kit (TaKaRa Bio Inc. #RR002A, Kyoto, Japan). .. Amplicons were visualised on a 1% agarose gel.

    Concentration Assay:

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. PCR conditions were investigated including the variation of the annealing temperature from 57 to 64°C, the concentration of primer sets from 0.08 to 0.56 μM, the concentration of La Taq DNA polymerase (TaKaRa, Dalian, China) from 0.75 to 1.75 U, and the concentration of PCR buffer (10×) and the dNTPs (2.5 mM) from 1.5 to 3 μL and 2 to 6 μL, respectively. ..

    Multiplex Assay:

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. Optimization of the Multiplex PCR After optimization, the optimum multiplex PCR assay was performed in a final volume of 25 μL, containing 2.5 μL of 10× PCR La buffer, 4 μL of dNTPs at 2.5 mM, 1.25 U of La Taq DNA polymerase, 0.32 μM of each primer, and 2 μL of the DNA template. ..

    Amplification:

    Article Title: Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)
    Article Snippet: .. The L. garvieae EPS synthesis gene cluster, as described in L. garvieae Lg2 (Miyauchi et al. ), was amplified in isolates A1–A3, A5, A6, A11 and A12 using the TaKaRa LA PCR Kit (TaKaRa Bio Inc. #RR002A, Kyoto, Japan). .. Amplicons were visualised on a 1% agarose gel.

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    TaKaRa la pcr buffer ii
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Pcr Buffer Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr buffer ii/product/TaKaRa
    Average 91 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    la pcr buffer ii - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    86
    TaKaRa la pcr reaction buffer ii
    <t>PCR</t> amplicons from mitochondrial <t>DNA</t> of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .
    La Pcr Reaction Buffer Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr reaction buffer ii/product/TaKaRa
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    la pcr reaction buffer ii - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Journal: International Journal of Molecular Sciences

    Article Title: Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

    doi: 10.3390/ijms14036436

    Figure Lengend Snippet: Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Article Snippet: PCR was performed with 0.06 units/μL LA Taq Hot-Start Version (Takara, Shiga, Japan), 0.03 units/μL KOD polymerase (Toyobo, Tokyo, Japan) or 40 nM A. aeolicus DnaE, in 1× Takara LA PCR buffer II (Takara, Shiga, Japan) containing 20 nM template DNA; 400 nM primers; 400 μM dATP, dTTP, dCTP and dGTP and various concentrations of Tth MutS or Aae MutS.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay

    Cytogenetic, FISH, and PCR analyses of the metastasis from the endometrial stromal sarcoma. A) Partial karyotype showing chromosome aberrations der(1)t(1;6)(p34;p21) and der(6)t(1;6)(p34;p21) together with the corresponding normal chromosomes; breakpoint position are indicated by arrows. B) FISH using BAC RP11-508M23 (green signal) from 1p34 containing the MEAF6 gene and a pool of the RP11-600P03 and RP11-436J22 BACs (red signal) from 6p21 containing the PHF1 gene. A part of the probe from 6p21 (red signal) has moved to the derivative chromosome 1, while the entire probe containing MEAF6 has moved to the derivative chromosome 6. The data suggest that the functional fusion gene is generated on the der(6). C) G-banding of the metaphase spread shown in (B). D) Amplification of a 1 kb cDNA in the 5′-RACE analysis (R) using reverse PHF1-721R and PHF1-526R primers and the universal forward primers. E) Partial sequence chromatograms of the 1 kb cDNA fragment showing the junctions (arrow) of MEAF6-PHF1 chimeric transcript (upper) and genomic hybrid DNA fragment (lower). F) RT-PCR and genomic PCR using specific MEAF6 and PHF1 primers. Lane 1: Amplification of MEAF6-PHF1 cDNA fragment with MEAF6-322F/PHF1-380R primers, lane 2: Amplification of MEAF6 transcript with MEAF6-15F/MEAF6-700R, lane 3: PHF1-18F/MEAF6-729R primer set did not amplify the reciprocal PHF1-MEAF6 cDNA, lane 4: Amplification of PHF1 transcript with PHF1-18F/PHF1-327R primers, lane 5: Amplification of MEAF6-PHF1 genomic hybrid DNA fragment with MEAF6-371F/PHF1-302R primer combination. M, 1 kb DNA ladder.

    Journal: PLoS ONE

    Article Title: Novel Fusion of MYST/Esa1-Associated Factor 6 and PHF1 in Endometrial Stromal Sarcoma

    doi: 10.1371/journal.pone.0039354

    Figure Lengend Snippet: Cytogenetic, FISH, and PCR analyses of the metastasis from the endometrial stromal sarcoma. A) Partial karyotype showing chromosome aberrations der(1)t(1;6)(p34;p21) and der(6)t(1;6)(p34;p21) together with the corresponding normal chromosomes; breakpoint position are indicated by arrows. B) FISH using BAC RP11-508M23 (green signal) from 1p34 containing the MEAF6 gene and a pool of the RP11-600P03 and RP11-436J22 BACs (red signal) from 6p21 containing the PHF1 gene. A part of the probe from 6p21 (red signal) has moved to the derivative chromosome 1, while the entire probe containing MEAF6 has moved to the derivative chromosome 6. The data suggest that the functional fusion gene is generated on the der(6). C) G-banding of the metaphase spread shown in (B). D) Amplification of a 1 kb cDNA in the 5′-RACE analysis (R) using reverse PHF1-721R and PHF1-526R primers and the universal forward primers. E) Partial sequence chromatograms of the 1 kb cDNA fragment showing the junctions (arrow) of MEAF6-PHF1 chimeric transcript (upper) and genomic hybrid DNA fragment (lower). F) RT-PCR and genomic PCR using specific MEAF6 and PHF1 primers. Lane 1: Amplification of MEAF6-PHF1 cDNA fragment with MEAF6-322F/PHF1-380R primers, lane 2: Amplification of MEAF6 transcript with MEAF6-15F/MEAF6-700R, lane 3: PHF1-18F/MEAF6-729R primer set did not amplify the reciprocal PHF1-MEAF6 cDNA, lane 4: Amplification of PHF1 transcript with PHF1-18F/PHF1-327R primers, lane 5: Amplification of MEAF6-PHF1 genomic hybrid DNA fragment with MEAF6-371F/PHF1-302R primer combination. M, 1 kb DNA ladder.

    Article Snippet: The 50 µL PCR volume contained 2 µL of cDNA, 1x LA PCR Buffer II (Mg2+ plus), 0.4 mM of each dNTP, 2.5 unit TaKaRa LA Taq (TaKaRA), and 0.6 µM of each of the forward and reverse primers.

    Techniques: Fluorescence In Situ Hybridization, Polymerase Chain Reaction, BAC Assay, Functional Assay, Generated, Amplification, Sequencing, Reverse Transcription Polymerase Chain Reaction

    Genomic divergences of the Revolver gene family offer chromosome tags . ( A ) PCR with the 3'-flanking region of a typical Revolver ( Revolver -2) used as a single primer amplified four DNA fragments (2.3 kb, 2.8 kb, 3.3 kb, and 4.3 kb) from the rye genome, but produced no fragments from the wheat genome. With this primer, rye chromosomes 1R and 5R can be identified in the wheat genome. ( B ) Each DNA fragment derived from the chromosome addition lines of 1R, 5R, and 6R (AB646252-646254) was a nonautonomous element of Revolver , with its second intron and the downstream region, but with considerable structural changes at the 5' end. Of the 440,000 Triticeae EST clones, only two clones, which were from T. aestivum (615 bp) and H. vulgare (713 bp), showed similarity with Revolver cDNA across its entire length. Other EST clones had low homology in the 5' exon 1 regions.

    Journal: BMC Evolutionary Biology

    Article Title: Genomic, RNA, and ecological divergences of the Revolver transposon-like multi-gene family in Triticeae

    doi: 10.1186/1471-2148-11-269

    Figure Lengend Snippet: Genomic divergences of the Revolver gene family offer chromosome tags . ( A ) PCR with the 3'-flanking region of a typical Revolver ( Revolver -2) used as a single primer amplified four DNA fragments (2.3 kb, 2.8 kb, 3.3 kb, and 4.3 kb) from the rye genome, but produced no fragments from the wheat genome. With this primer, rye chromosomes 1R and 5R can be identified in the wheat genome. ( B ) Each DNA fragment derived from the chromosome addition lines of 1R, 5R, and 6R (AB646252-646254) was a nonautonomous element of Revolver , with its second intron and the downstream region, but with considerable structural changes at the 5' end. Of the 440,000 Triticeae EST clones, only two clones, which were from T. aestivum (615 bp) and H. vulgare (713 bp), showed similarity with Revolver cDNA across its entire length. Other EST clones had low homology in the 5' exon 1 regions.

    Article Snippet: Reaction mixtures contained 10 ng of template genomic DNA, 50 pmoles of single primer (5'-GTAGTCGTCAGGAGTCCTCACCA-3'), 0.4 mM dNTPs, 1 × LA PCR buffer II, 2.5 mM MgCl2 , and 0.5 U of LA Taq polymerase (Takara) in a volume of 50 μL.

    Techniques: Polymerase Chain Reaction, Amplification, Produced, Derivative Assay, Clone Assay

    PCR amplicons from mitochondrial DNA of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .

    Journal: BMC Genomics

    Article Title: Evolution of multipartite mitochondrial genomes in the booklice of the genus Liposcelis (Psocoptera)

    doi: 10.1186/1471-2164-15-861

    Figure Lengend Snippet: PCR amplicons from mitochondrial DNA of Liposcelis entomophila (A) and L. paeta (B). Lane M: 1 kb marker (Biomed). “E1-E2”, the product of PCR with primers E1 and E2, etc. Details of primers are in Additional files 1 and 2 .

    Article Snippet: Each long PCR reaction is 25 μL in volume, containing 1.0 μL each of forward primer (10 μM) and reverse primer (10 μM), 4.0 μL of dNTPs mix (each 2.5 mM), 1.0 μL of template DNA, 2.5 μL MgCl2 (25 mM), 2.5 μL of 10 × LA PCR reaction buffer II, 12.75 μL ddH2 O and 0.25 μL LA Taq DNA polymerase (5 U/μL, Takara).

    Techniques: Polymerase Chain Reaction, Marker