Journal: Frontiers in Microbiology
Article Title: Prevalence and detection of Stenotrophomonas maltophilia carrying metallo-β-lactamase blaL1 in Beijing, China
Figure Lengend Snippet: Specificity of the LAMP method for bla L1 gene detection. It has two parts, (A) is the graphic and (B) is the photography of microtubes. The reaction proceeded at 65°C for 65 min. Turbidity was monitored in the Loopamp real-time turbidimeter and the OD (λ650nm) recorded at 6 s intervals. L1, Brucella suis 3572; L2, Bacillus megatherium 4623; L3, Vibrio carchariae 5732; L4, Acinetobacter baumannii B260; L5, Corynebacterium diphtheriae CMCC38001; L6, Acinetobacter baumannii H18; L7, Mycobacterium tuberculosis 8362; L8, Shigella sonnei 2531; L9, Shigella flexneri 4536; L10, Salmonella enteritidis 50326-1; L11, Yersinia enterocolitica 1836; L12, Vibrio parahaemolyticus 5474; L13, Salmonella paratyphi 86423; L14, Neisseria meningitidis group B CMCC29022; L15, Enterotoxigenic E. coli 44824; L16, Beta hemolytic Streptococcus group A CMCC32213; L17, Yersinia pestis 2638; L18, Salmonella aberdeen 9264; L19, Vibrio cholera 3802; L20, Staphylococcus aureus 2740; L21, Bordetella pertussis ATCC 18530 ; L22, positive control ( S. maltophilia - K279a); L23, negative control (distilled water).
Article Snippet: Real-time changes in turbidity were monitored by measuring the optical density (λ650 nm ) at 6 s intervals, for each LAMP reaction in a Loopamp real-time turbidimeter (LA-320c; Eiken Chemical Co., Ltd.).
Techniques: Positive Control, Negative Control