Journal: Computational and Structural Biotechnology Journal

Article Title: Resolving the role of podoplanin in the motility of papillary thyroid carcinoma-derived cells using RNA sequencing

doi: 10.1016/j.csbj.2023.07.035

Figure Lengend Snippet: Primary antibodies used in the study for Western blotting.

Article Snippet: tSRC/ 2110 S , Mouse monoclonal (IgG1)/ L4A1 , 1:1000/ 5% skimmed milk , Cell Signaling Technology, Inc..

Techniques: Western Blot

Journal: Cell reports

Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling

doi: 10.1016/j.celrep.2023.112539

Figure Lengend Snippet: (A) Domain structure of human c-Src protein (Uniprot: P12931) with key regulatory sites. From the N (amino) to the C (carboxyl) terminus, domains include the N-terminal SRC homology SH4 with the unique (U) (brown), SH3 (blue), SH2 (orange), kinase domain (KD; SH1) (green), and C-terminal regulatory (R) (red) domains. Domain range is indicated with residue numbers, shown below each domain (above for SH3). (B) Schematic representation of C-terminal c-Src truncated constructs (untagged) used in (C). Red asterisks: proteins detected in conditioned media (CMs). (C) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using an anti-N-term-c-Src (N-term, N-terminal) antibody (mAb3) (see ). (D) Schematic representation of N-term c-Src truncations from (E). Red asterisks: proteins detected in CMs. (E) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody (mAb4). (F) HEK293 cells were transiently transfected with c-Src. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src or anti-phos-Y530-Src. (G) Cell extracts and CMs from transiently transfected HEK293 cells. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src antibody. (H) Schematic diagram displaying how c-Src truncations impact on its secretion. Arrows indicate secretion. Red arrow indicates secretion is hindered. MW, molecular weight; WT, wild type; EV, empty vector; Ab, antibody. Empty vector (EV) was used as a control. GAPDH and Coomassie blue staining were used in all immunoblots as loading controls. See also .

Article Snippet: Mouse anti-Src mAb3 (L4A1) , Cell Signaling Technology , Cat#2110; RRID:AB_10691385.

Techniques: Residue, Construct, Transfection, Expressing, Western Blot, Molecular Weight, Plasmid Preparation, Staining

Journal: Cell reports

Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling

doi: 10.1016/j.celrep.2023.112539

Figure Lengend Snippet:

Article Snippet: Mouse anti-Src mAb3 (L4A1) , Cell Signaling Technology , Cat#2110; RRID:AB_10691385.

Techniques: Recombinant, Virus, Bradford Assay, Staining, Colorimetric Assay, Software

Journal: PLoS ONE

Article Title: Does a rare mutation in PTPRA contribute to the development of Parkinson’s disease in an Australian multi-incident family?

doi: 10.1371/journal.pone.0271499

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Src (L4A1) , Cell Signalling , #2110 , 1:3000.

Techniques:

Journal: Cell reports

Article Title: A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand

doi: 10.1016/j.celrep.2022.111039

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-Src (L4A1) , Cell Signaling Technology , Cat# 2110; RRID:AB_10691385.

Techniques: Recombinant

Journal: eLife

Article Title: EGFR transactivates RON to drive oncogenic crosstalk

doi: 10.7554/eLife.63678

Figure Lengend Snippet:

Article Snippet: antibody , Anti-Src (Mouse monoclonal) , Cell Signaling Technology , Cat# 2110 RRID: AB_10691385 , Clone L4A1 WB (1:2000).

Techniques: Stable Transfection, Transfection, Plasmid Preparation, Construct, Generated, Cytometry, Kinase Assay, Recombinant, Sequencing, Ligation, Mutagenesis, Bicinchoninic Acid Protein Assay, Expressing, Software, Magnetic Beads