dope  (Echelon Biosciences)


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    Echelon Biosciences dope
    Dope, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dope/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
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    dope - by Bioz Stars, 2023-01
    93/100 stars

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    dope  (Echelon Biosciences)


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  • 93

    Structured Review

    Echelon Biosciences dope
    Dope, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dope/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dope - by Bioz Stars, 2023-01
    93/100 stars

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    dope  (Echelon Biosciences)


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  • 93

    Structured Review

    Echelon Biosciences dope
    PI(3)P-dependent lipid binding of hSNX16 CC domain mutants. (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PX-CC dimer (PDB accession number 5GW0 ; ). CC domains are shown in green and glutamates in magenta. (C–F) Liposome cosedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in C and E. (C and D) Liposomes composed of <t>80%</t> <t>DOPC</t> (1,2-dioleoyl-sn-glycero-3-phosphocholine), 15% <t>DOPE</t> (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), 5% DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in DOPC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild-type hSNX16. Y145A mutsation abolishes PI(3)P binding of hSNX16 as previously reported . (E and F) Binding of wild-type hSNX16 is more salt sensitive than hSNX16 3A . Liposomes (70% DOPC, 15% DOPE, 5% DOPS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using a Kruskal–Wallis test followed by a Dunn’s multiple comparisons test. Data are presented as mean ± SEM. *, P < 0.05. ns, not significant.
    Dope, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dope/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dope - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Higher-order assembly of Sorting Nexin 16 controls tubulation and distribution of neuronal endosomes"

    Article Title: Higher-order assembly of Sorting Nexin 16 controls tubulation and distribution of neuronal endosomes

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201811074

    PI(3)P-dependent lipid binding of hSNX16 CC domain mutants. (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PX-CC dimer (PDB accession number 5GW0 ; ). CC domains are shown in green and glutamates in magenta. (C–F) Liposome cosedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in C and E. (C and D) Liposomes composed of 80% DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), 15% DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), 5% DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in DOPC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild-type hSNX16. Y145A mutsation abolishes PI(3)P binding of hSNX16 as previously reported . (E and F) Binding of wild-type hSNX16 is more salt sensitive than hSNX16 3A . Liposomes (70% DOPC, 15% DOPE, 5% DOPS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using a Kruskal–Wallis test followed by a Dunn’s multiple comparisons test. Data are presented as mean ± SEM. *, P < 0.05. ns, not significant.
    Figure Legend Snippet: PI(3)P-dependent lipid binding of hSNX16 CC domain mutants. (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PX-CC dimer (PDB accession number 5GW0 ; ). CC domains are shown in green and glutamates in magenta. (C–F) Liposome cosedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in C and E. (C and D) Liposomes composed of 80% DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), 15% DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), 5% DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in DOPC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild-type hSNX16. Y145A mutsation abolishes PI(3)P binding of hSNX16 as previously reported . (E and F) Binding of wild-type hSNX16 is more salt sensitive than hSNX16 3A . Liposomes (70% DOPC, 15% DOPE, 5% DOPS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using a Kruskal–Wallis test followed by a Dunn’s multiple comparisons test. Data are presented as mean ± SEM. *, P < 0.05. ns, not significant.

    Techniques Used: Binding Assay, Construct, Purification, Incubation, Staining, Concentration Assay

    dope  (Echelon Biosciences)


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    Echelon Biosciences dope
    (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PXCC dimer (PDB# 5gw0; . CC domains are shown in green, and glutamates in magenta. (C-F) Liposome co-sedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in (C) and (E). (C, D) Liposomes composed of <t>80%</t> <t>DOPC,</t> 15% <t>DOPE,</t> 5% DOPS, and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in PC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild type hSNX16. Y145A mutation abolishes PI(3)P binding of hSNX16 as previously reported . (E, F) Binding of wild type hSNX16 is more salt-sensitive than hSNX16 3A . Liposomes (70% PC, 15% PE, 5% PS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using Kruskal-Wallis test followed by Dunn’s multiple comparisons test. Data are represented as mean ± SEM. *p < 0.05.
    Dope, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dope/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dope - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Higher order assembly of Sorting Nexin 16 controls tubulation and distribution of neuronal endosomes"

    Article Title: Higher order assembly of Sorting Nexin 16 controls tubulation and distribution of neuronal endosomes

    Journal: bioRxiv

    doi: 10.1101/469932

    (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PXCC dimer (PDB# 5gw0; . CC domains are shown in green, and glutamates in magenta. (C-F) Liposome co-sedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in (C) and (E). (C, D) Liposomes composed of 80% DOPC, 15% DOPE, 5% DOPS, and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in PC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild type hSNX16. Y145A mutation abolishes PI(3)P binding of hSNX16 as previously reported . (E, F) Binding of wild type hSNX16 is more salt-sensitive than hSNX16 3A . Liposomes (70% PC, 15% PE, 5% PS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using Kruskal-Wallis test followed by Dunn’s multiple comparisons test. Data are represented as mean ± SEM. *p < 0.05.
    Figure Legend Snippet: (A) Schematic of hSNX16 constructs. (B) Location of hSNX16 CC mutations on hSNX16 PXCC dimer (PDB# 5gw0; . CC domains are shown in green, and glutamates in magenta. (C-F) Liposome co-sedimentation assays. Purified hSNX16 variants were incubated with liposomes of the indicated composition and pelleted. Representative Coomassie staining of supernatant (S) and pellet (P) fractions is shown in (C) and (E). (C, D) Liposomes composed of 80% DOPC, 15% DOPE, 5% DOPS, and PI(3)P (0.5%, 1%, 2.5%, 5%, and 10%; with a corresponding decrease in PC). PI(3)P-dependent lipid binding of purified hSNX16 CC domain mutants is not significantly different from wild type hSNX16. Y145A mutation abolishes PI(3)P binding of hSNX16 as previously reported . (E, F) Binding of wild type hSNX16 is more salt-sensitive than hSNX16 3A . Liposomes (70% PC, 15% PE, 5% PS, and 10% PI(3)P) were incubated for 45 min with purified hSNX16 and the indicated NaCl concentrations before pelleting. In the last condition, hSNX16 and liposomes were incubated in 100 mM NaCl for 30 min, and then NaCl was added to a final concentration of 400 mM for 15 min before pelleting. Quantification is a result of three independent experiments, analyzed using Kruskal-Wallis test followed by Dunn’s multiple comparisons test. Data are represented as mean ± SEM. *p < 0.05.

    Techniques Used: Construct, Sedimentation, Purification, Incubation, Staining, Binding Assay, Mutagenesis, Concentration Assay

    dioleoyl sn glycero 3 phosphoethanolamine dope  (Echelon Biosciences)


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    Echelon Biosciences dioleoyl sn glycero 3 phosphoethanolamine dope
    Dioleoyl Sn Glycero 3 Phosphoethanolamine Dope, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dioleoyl sn glycero 3 phosphoethanolamine dope/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
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    dioleoyl sn glycero 3 phosphoethanolamine dope - by Bioz Stars, 2023-01
    93/100 stars

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    l 2182 pi  (Echelon Biosciences)


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    Echelon Biosciences l 2182 pi
    L 2182 Pi, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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