dsl  (Vector Laboratories)


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    Vector Laboratories dsl
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    dsl  (Vector Laboratories)


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    Vector Laboratories dsl
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    d stramonium dsl  (Vector Laboratories)


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    Vector Laboratories d stramonium dsl
    D Stramonium Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    datura stramonium lectin dsl  (Vector Laboratories)


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    Vector Laboratories datura stramonium lectin dsl
    Datura Stramonium Lectin Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dsl  (Vector Laboratories)


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    Vector Laboratories dsl
    Spores have specific lectin binding properties. Spores and yeast cells were stained <t>with</t> <t>calcofluor</t> white (to stain all cell types) and fluorescently labeled lectins (to show spore-specific binding). Stained cells were visualized by fluorescence microscopy. Bars, 5 μm. (A) Spore-specific staining by <t>DSL</t> (green), which binds N-acetylglucosamine oligomers. The left panel shows a yeast cell (∼5 μm in diameter; blue) and two spores (∼3 μm long; green). The right panel shows a long filament cell with a terminal basidium (club-like structure of ∼5 μm in diameter; blue), with spores from collapsed chains on the end and scattered along the filament (∼3 μm long; green). (B) Spore-specific staining by ConA (visualized in red in the left panel in and green in the right panel), which binds α-mannose residues. The left panel shows a yeast cell (∼5 μm in diameter; blue) and a spore (∼3 μm long; red). The right panel shows a basidium (club-like structure of ∼5 μm in diameter; blue) with collapsed spore chains attached (∼3 μm long; green).
    Dsl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsl/product/Vector Laboratories
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    1) Product Images from "Isolation and Characterization of Cryptococcus neoformans Spores Reveal a Critical Role for Capsule Biosynthesis Genes in Spore Biogenesis"

    Article Title: Isolation and Characterization of Cryptococcus neoformans Spores Reveal a Critical Role for Capsule Biosynthesis Genes in Spore Biogenesis

    Journal:

    doi: 10.1128/EC.00352-08

    Spores have specific lectin binding properties. Spores and yeast cells were stained with calcofluor white (to stain all cell types) and fluorescently labeled lectins (to show spore-specific binding). Stained cells were visualized by fluorescence microscopy. Bars, 5 μm. (A) Spore-specific staining by DSL (green), which binds N-acetylglucosamine oligomers. The left panel shows a yeast cell (∼5 μm in diameter; blue) and two spores (∼3 μm long; green). The right panel shows a long filament cell with a terminal basidium (club-like structure of ∼5 μm in diameter; blue), with spores from collapsed chains on the end and scattered along the filament (∼3 μm long; green). (B) Spore-specific staining by ConA (visualized in red in the left panel in and green in the right panel), which binds α-mannose residues. The left panel shows a yeast cell (∼5 μm in diameter; blue) and a spore (∼3 μm long; red). The right panel shows a basidium (club-like structure of ∼5 μm in diameter; blue) with collapsed spore chains attached (∼3 μm long; green).
    Figure Legend Snippet: Spores have specific lectin binding properties. Spores and yeast cells were stained with calcofluor white (to stain all cell types) and fluorescently labeled lectins (to show spore-specific binding). Stained cells were visualized by fluorescence microscopy. Bars, 5 μm. (A) Spore-specific staining by DSL (green), which binds N-acetylglucosamine oligomers. The left panel shows a yeast cell (∼5 μm in diameter; blue) and two spores (∼3 μm long; green). The right panel shows a long filament cell with a terminal basidium (club-like structure of ∼5 μm in diameter; blue), with spores from collapsed chains on the end and scattered along the filament (∼3 μm long; green). (B) Spore-specific staining by ConA (visualized in red in the left panel in and green in the right panel), which binds α-mannose residues. The left panel shows a yeast cell (∼5 μm in diameter; blue) and a spore (∼3 μm long; red). The right panel shows a basidium (club-like structure of ∼5 μm in diameter; blue) with collapsed spore chains attached (∼3 μm long; green).

    Techniques Used: Binding Assay, Staining, Labeling, Fluorescence, Microscopy

    Capsule mutants have defects in sexual development and spore formation. (A) All panels show the periphery of a cross on V8 medium under a light microscope at low magnification (×200). Insets each show a single basidium under higher magnification (×400). (1) Wild-type a × α cross. (2 and 3) Wild-type a or α strain crossed with corresponding a cap67 or α cap67 strain. (4) a cap67 × α cap67 cross. The few basidia that were produced did not develop proper spore chains and yielded clumps of spores (inset in panel 4). Identical results were obtained for all additional capsule deletion strains tested (cap10Δ, cap60Δ, and cap64Δ strains). (B) Spores were stained with DAPI (blue) and DSL (green) and visualized by fluorescence micros- copy. (Left) Normal morphology of spores derived from a wild-type cross. (Right) Spores derived from a cap67 × cap67 cross forming an aggregated mass of spores. (C) Spores from wild-type and cap67 strains stained with DAPI to reveal nuclei (blue), with anti-GXM antibody (F12D2) to detect capsule (red), and with DSL to identify spores (green) were visualized by immunofluorescence microscopy. All three color channels were merged into a single image for each sample. (1) Spores derived from a wild-type a by α cross (JEC20 × JEC21). (2 and 3) Spores and yeast cells from crosses between wild-type and cap67 strains (CHY1377 × JEC21 and JEC20 × ATCC 52817, respectively). (4) Spores and yeast cells isolated from a cross between two cap67 strains (CHY1377 × ATCC 52817). Bars, 5 μm.
    Figure Legend Snippet: Capsule mutants have defects in sexual development and spore formation. (A) All panels show the periphery of a cross on V8 medium under a light microscope at low magnification (×200). Insets each show a single basidium under higher magnification (×400). (1) Wild-type a × α cross. (2 and 3) Wild-type a or α strain crossed with corresponding a cap67 or α cap67 strain. (4) a cap67 × α cap67 cross. The few basidia that were produced did not develop proper spore chains and yielded clumps of spores (inset in panel 4). Identical results were obtained for all additional capsule deletion strains tested (cap10Δ, cap60Δ, and cap64Δ strains). (B) Spores were stained with DAPI (blue) and DSL (green) and visualized by fluorescence micros- copy. (Left) Normal morphology of spores derived from a wild-type cross. (Right) Spores derived from a cap67 × cap67 cross forming an aggregated mass of spores. (C) Spores from wild-type and cap67 strains stained with DAPI to reveal nuclei (blue), with anti-GXM antibody (F12D2) to detect capsule (red), and with DSL to identify spores (green) were visualized by immunofluorescence microscopy. All three color channels were merged into a single image for each sample. (1) Spores derived from a wild-type a by α cross (JEC20 × JEC21). (2 and 3) Spores and yeast cells from crosses between wild-type and cap67 strains (CHY1377 × JEC21 and JEC20 × ATCC 52817, respectively). (4) Spores and yeast cells isolated from a cross between two cap67 strains (CHY1377 × ATCC 52817). Bars, 5 μm.

    Techniques Used: Light Microscopy, Produced, Staining, Fluorescence, Derivative Assay, Immunofluorescence, Microscopy, Isolation

    Capsule is not efficiently transferred to the surfaces of capsule-deficient spores. Yeast cells and spores were labeled with DAPI (blue) to reveal nuclei, with DSL (green) to identify spores, and with anti-GXM antibody F12D2 (red) to highlight polysaccharide epitopes. The top row of panels shows yeast cells and spores from a wild-type cross. Note that wild-type spores and yeast cells have similar intensities of staining with anti-GXM antibody. The middle row shows yeast cells and spores from an a cap67 × α cap67 cross, in which no anti-GXM antibody staining is detectable. The bottom row of panels shows yeast cells and spores stained after incubation in capsule-conditioned medium. Anti-GXM antibody staining shows brightly stained yeast cells, with only faint staining of the spores. The final panel in each row is a merged image of all three fluorescence channels used.
    Figure Legend Snippet: Capsule is not efficiently transferred to the surfaces of capsule-deficient spores. Yeast cells and spores were labeled with DAPI (blue) to reveal nuclei, with DSL (green) to identify spores, and with anti-GXM antibody F12D2 (red) to highlight polysaccharide epitopes. The top row of panels shows yeast cells and spores from a wild-type cross. Note that wild-type spores and yeast cells have similar intensities of staining with anti-GXM antibody. The middle row shows yeast cells and spores from an a cap67 × α cap67 cross, in which no anti-GXM antibody staining is detectable. The bottom row of panels shows yeast cells and spores stained after incubation in capsule-conditioned medium. Anti-GXM antibody staining shows brightly stained yeast cells, with only faint staining of the spores. The final panel in each row is a merged image of all three fluorescence channels used.

    Techniques Used: Labeling, Staining, Incubation, Fluorescence

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